BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0507.p (289 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the T... 156 7e-38 mll5767 Non COG 91 4e-18 mlr1708 Non COG 87 4e-17 AGl2610 [R] COG3618 Predicted metal-dependent hydrolase of the T... 75 1e-13 BMEI1718 [R] COG3618 Predicted metal-dependent hydrolase of the ... 74 3e-13 PA2211 [R] COG3618 Predicted metal-dependent hydrolase of the TI... 69 9e-12 SMb21101 [R] COG3618 Predicted metal-dependent hydrolase of the ... 62 1e-09 mlr7156 Non COG 61 3e-09 >AGc5105 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 287 Score = 156 bits (394), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 102/285 (35%), Positives = 141/285 (49%), Gaps = 17/285 (5%) Query: 6 IDAHQHFWFYQPQAYPWIGAGMELLACDRTPGQLQPLLDAEGLGQSIAVQARAGREETGF 65 IDAHQHFW + +A W + + D P L PLL A G+ ++ VQ +T F Sbjct: 17 IDAHQHFWLMKDRAGQWPPPSLAAIYRDFLPTDLSPLLTAAGVSGTVLVQTMETAADTDF 76 Query: 66 LLELARGNRRIAGVVGWEDLASPHLAANVERWGRDK-LVGFRHQLQDEADVGGCVDAPAF 124 +LELA I GVVGW D+ S + A + R + G R LQ DV DA A Sbjct: 77 MLELAENTDFIKGVVGWVDMKSAEVTAEIARRAKHPAFKGVRPMLQGMEDVAWIDDA-AL 135 Query: 125 NAGVAWLQRQGMVYDVLVYARQMAEVQAFCARHDRHWLVLDHLGKPALTEFGRGDTAFEG 184 NA V L + G+V+D LV + + +F ++H +V+DH KP + + Sbjct: 136 NAAVEALVKHGLVFDALVLPPNLPHLLSFASKHPELPVVIDHGAKPLI-----ATGHYRD 190 Query: 185 WQRQLRALAALPHVACKLSGLVTEADWQRGLRQKDFDNISLCLDTALEAFGPQRLMFGSD 244 W+R + ALAA P+V CKLSGL+TE QR + + +T LE FG R++FGSD Sbjct: 191 WRRNMAALAARPNVHCKLSGLLTERGEQRP------EAVRPYAETILELFGGDRVIFGSD 244 Query: 245 WPVCLLAASYAKVAGVVREWAATRLSAAEQELLWGATAARCYGLA 289 WPV LA + R+ + AA ++G A R Y L Sbjct: 245 WPVLRLAGDFQGWLDFCRDIVPAKDHAA----VFGGNAIRFYSLT 285 >mll5767 Non COG Length = 297 Score = 90.5 bits (223), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 86/306 (28%), Positives = 129/306 (42%), Gaps = 34/306 (11%) Query: 4 PRIDAHQHFWFYQPQAYPWIGAGMELLA-----CDRTPGQLQPLL-DAEGLGQSIAVQAR 57 P ID H H W + YPW+ G++ A RT +L L DA +V Sbjct: 5 PFIDPHHHLWDLEVNYYPWLTDGVKPSAFGDYEAIRTNYRLDDYLEDARNQNLVKSVHLD 64 Query: 58 AGRE------ETGFLLELARGNRRIAGVVGWEDLASPHLAANVERWGR-DKLVGFRHQLQ 110 G + ET +L E+A G+VG+ DL+ P + ++R G R + Sbjct: 65 VGFDPSDPVGETRWLQEIANKRGFPHGIVGYADLSKPDVGELLDRHMEYPNFRGIRQSMN 124 Query: 111 DEADVGGCVDA-------PAFNAGVAWLQRQGMVYDVLVYARQMAEVQAFCARHDRHWLV 163 AD A P + G L ++ + +D+ +Y QM E ++ Sbjct: 125 YHADPAKTYQARPEVSRTPEWRRGFRELAKRRLSFDLQLYYPQMEEFLQLAHDFPDVQII 184 Query: 164 LDHLGKPALTEFGRGDTAFEGWQRQLRALAALPHVACKLSGLVTEADWQRGLRQKDFDNI 223 L+H G G F W++ + LA P+VACK+SGL DW +I Sbjct: 185 LNHTGMQV-----DGPKHFNAWKKGMHRLAQAPNVACKISGLGM-GDWSWTTA-----SI 233 Query: 224 SLCLDTALEAFGPQRLMFGSDWPVCLLAASYAKVAGVVREWAATRLSAAEQEL-LWGATA 282 ++ A+ AFG R MF +++PV L +S+ V +E TR E+ L L+ A Sbjct: 234 RPYVEEAIAAFGVGRSMFATNFPVDKLFSSFDAVVNAFKE--ITRYYPHEERLALFHDNA 291 Query: 283 ARCYGL 288 AR Y L Sbjct: 292 ARHYRL 297 >mlr1708 Non COG Length = 295 Score = 87.0 bits (214), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 83/302 (27%), Positives = 133/302 (44%), Gaps = 35/302 (11%) Query: 6 IDAHQHFWFYQPQAYPWIGAGM--------ELLACDRTPGQLQPLLDAEGLGQSIAVQAR 57 IDAH H W + Q PW+ M E + D G+ G+ +++ VQ Sbjct: 5 IDAHFHIWRQKDQ--PWLVGPMVPRIFGPYEPIRRDYPIGEFLQDQKGSGVEKAVYVQTN 62 Query: 58 AGRE----ETGFLLELARGNRRIAGVVGWEDLASPHLAANVERWGRDKLV-GFRHQL--- 109 +E E FL + A +VG+ D+ + ++R + +L+ G R QL Sbjct: 63 WAKEDFEKEVAFLQQTADETGWPHAIVGYADMTVDDVRPQIDRLMKYRLLRGVRMQLHWH 122 Query: 110 -----QDEADVGGCVDAPAFNAGVAWLQRQGMVYDVLVYARQMAEVQAFCARHDRHWLVL 164 + A +D P A VA L+ G+ +D+ ++ QM + + +L Sbjct: 123 ETPAFRFAASADQVID-PKVRANVARLKDYGLSFDLQLFPAQMKDGVTLVGENPETNFIL 181 Query: 165 DHLGKPALTEFGRGDTAFEGWQRQLRALAALPHVACKLSGLVTEADWQRGLRQKDFDNIS 224 H G LT G E W+ LR L+A P+ KLSGL T + + D I+ Sbjct: 182 THAG--MLT--GMEPEVTETWKAGLRTLSAAPNFYAKLSGLGT------FVHRNDPALIA 231 Query: 225 LCLDTALEAFGPQRLMFGSDWPVCLLAASYAKVAGVVREWAATRLSAAEQELLWGATAAR 284 +D A++ G LMFGS++P+ L S+A++ R+ A +AAE ++ W TA + Sbjct: 232 YIVDNAIDILGADHLMFGSNFPIEKLWTSHAELIKAHRDAVARHGAAAEADIFWN-TAEK 290 Query: 285 CY 286 Y Sbjct: 291 VY 292 >AGl2610 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 297 Score = 75.5 bits (184), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 78/293 (26%), Positives = 124/293 (42%), Gaps = 31/293 (10%) Query: 6 IDAHQHFWFYQPQAYPWIGAGMELLACDRTPGQLQPLLDAEGLGQSIAVQARAGREETGF 65 ID HQH Y W G LA T L++ G+ +I ++ G ++ + Sbjct: 21 IDTHQHLILRGKLGYAWT-RGEPSLAGSFTRADYAALVEGSGVIGTIFMET--GVDDGDY 77 Query: 66 LLELARGNRRIAGVVG------WEDLAS--PHLAANVERWGRD----KLVGFRHQLQDEA 113 E R +AG+VG + +AS P + W + K+VGFR LQ Sbjct: 78 RAE----ARLVAGMVGEGTLPMFGQIASCRPETDQGFDAWLEECRHLKVVGFRRILQVMP 133 Query: 114 DVGGCVDAPAFNAGVAWLQRQGMVYDVLVYARQMAEVQAFCARHDRHWLVLDHLGKPALT 173 D A + V + + G D+ ++RQ+A +LDH G P + Sbjct: 134 D--DTSRAENYRRNVRKIGKAGWPVDLCFHSRQLAIAAELVRACPDVRFMLDHCGTPDI- 190 Query: 174 EFGRGDTAFEGWQRQLRALAALPHVACKLSGLVTEADWQRGLRQKDFDNISLCLDTALEA 233 F W ++ LA+LP++ KLSG+ Q +++ ++T L+ Sbjct: 191 ----AGNDFPAWSERMADLASLPNLFVKLSGVTAYC----APGQVTLESVRPYVETVLKL 242 Query: 234 FGPQRLMFGSDWPVCLLAASYAKVAGVVREWAATRLSAAEQELLWGATAARCY 286 FGP R+++G DWPV L + + + RE+ +T LS EQ + A R Y Sbjct: 243 FGPARMVWGGDWPVVDLGSGLPEWIAMTREFLST-LSPDEQASIGHGNARRFY 294 >BMEI1718 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Bme Brucella melitensis Length = 282 Score = 73.9 bits (180), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 78/296 (26%), Positives = 122/296 (41%), Gaps = 29/296 (9%) Query: 6 IDAHQHFWFYQPQAYPWIGAGMEL---LACDRTPGQLQPLLDAEGLGQSIAVQARAGREE 62 ID H H +YPW+ + L D Q + L + V A + E Sbjct: 3 IDTHLHLIDKNALSYPWLESAPALNRDFLFDSYRLQAERAGIGGALHIEVDVAPHAMQAE 62 Query: 63 TGFLLELAR-GNRRIAGVVGWEDLASPHLAANVERWGRDKLV-GFR---HQLQDEADVGG 117 T + ++AR I G + P AA +ER D V G R H + DE G Sbjct: 63 TDHIRQIARRSGGFIKGAIASCRPEEPGFAAYLERQLSDPFVKGLRRVLHVVPDEVSEGA 122 Query: 118 CVDAPAFNAGVAWLQRQGMVYDVLVYARQMAEVQAFCARHDRHWLVLDHLGKPALTEFGR 177 F + L+ G+ +D+ V Q+ + A ++DH G P + Sbjct: 123 L-----FRENIKRLEGTGLTFDLCVQPHQIGKAIALADLAPDLCFIVDHCGAPDIKA--- 174 Query: 178 GDTAFEGWQRQLRALAALPHVACKLSGLVTEADWQRGLRQKDFDNISLCLDTALEAFGPQ 237 A +GW+ + ++ P+V K+SGLV D + + + + +++FG Sbjct: 175 --GALDGWREGMAEISRRPNVIAKISGLVVYCDAE----SWTVETLRPYAEQVIQSFGWD 228 Query: 238 RLMFGSDWPVCLLAASYAKVAGVVREWAATR-----LSAAEQELLWGATAARCYGL 288 R+++GSDWPVC LA+S AG+ AAT S E+ L+ A R + L Sbjct: 229 RVIWGSDWPVCTLASS--PDAGLSTWVAATHALLEGCSTTEKSRLFCENARRIWNL 282 >PA2211 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Pae Pseudomonas aeruginosa Length = 319 Score = 69.3 bits (168), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 77/303 (25%), Positives = 126/303 (41%), Gaps = 31/303 (10%) Query: 4 PRIDAHQHFWFYQPQAYPWIGAGMEL---------LACDRTPGQLQPLLDAEGLGQSIAV 54 P +DAH HFW Q +PW+ ++ + P + A + +++ V Sbjct: 8 PIVDAHHHFWDPQANYHPWLAEDAKIPFRYGDYSAIKRRYLPADYFGDVGAHRVVETVYV 67 Query: 55 QA----RAGREETGFLLELARGNRRIAGVVGWEDLASPHLAANV-ERWGRDKLVGFRHQ- 108 + R ET F+ LA + VV L +P A + + G ++ RH+ Sbjct: 68 ETEWDPRDPLGETRFVHHLAERHGAPHAVVAQAWLDAPDAAEVLAAQAGFARVRSVRHKP 127 Query: 109 -----LQDEADVGGCVDAPAFNAGVAWLQRQGMVYDVLVYARQMAEVQAFCARHDRHWLV 163 Q ++ + + G A L R G+ +D+ +AE +V Sbjct: 128 GGPQRPQQVGELRSLMSDERWRRGYAELARHGLHFDLQTPWWNLAEAACLARDFPDTLIV 187 Query: 164 LDHLGKPALTEFGRGDTAFEGWQRQLRALAALPHVACKLSGLVTEADWQRGLRQKDFDNI 223 L+H G P+ R + WQR + A P+VA K+SG+ Q G R DN Sbjct: 188 LNHAGLPS----DRSEEGLAAWQRAMARFAECPNVALKISGIG-----QAGRRWSVEDNA 238 Query: 224 SLCLDTALEAFGPQRLMFGSDWPVCLLAASYAKVAGVVREWAATRLSAAEQELLWGATAA 283 + ++ + FG +R MF S++PV L S+ + G + A L A+QE L+ + A Sbjct: 239 WIVRES-IALFGVERAMFASNFPVDSLCGSFDDIYGGFKRIVAD-LPYADQERLFHSNAR 296 Query: 284 RCY 286 R Y Sbjct: 297 RIY 299 >SMb21101 [R] COG3618 Predicted metal-dependent hydrolase of the TIM-barrel fold Sme Sinorhizobium meliloti Length = 278 Score = 62.4 bits (150), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 69/264 (26%), Positives = 103/264 (39%), Gaps = 35/264 (13%) Query: 6 IDAHQHFWFYQPQAYPWIGAGMELLACDRTPGQLQPLLDAEGLGQSIAVQARAGREETGF 65 ID H H YPW+ + A DR +A+ +G + ++ + Sbjct: 3 IDTHLHLIDKSALNYPWLAG---VPALDRDFLYATYAAEAKRVGVAASLHMEVDVDPAEI 59 Query: 66 LLELARGNRRIAGVVGW-----------EDLASPHLAANVERWGRDKLV-GFRHQLQDEA 113 LE R R+AG G ED AA +ER + V GFR L Sbjct: 60 ELE-TREVARLAGEPGSLLKGAIAACRPEDEG---FAAYLERQEENAFVKGFRRVLHVVT 115 Query: 114 DVGGCVDAPAFNAGVAWLQRQGMVYDVLVYARQMAEVQAFCARHDRHWLVLDHLGKPALT 173 D + P F V L +D+ V Q+ + A +LDH G P + Sbjct: 116 D--DLSEQPLFRENVKRLSGTRFTFDLCVLPHQIPKAIALADLAPDVQFILDHCGVPDIK 173 Query: 174 EFGRGDTAFEGWQRQLRALAALPHVACKLSGLVT--EADWQRGLRQKDFDNISLCLDTAL 231 A W+ + +A P+V K+SG+V E DW D+I ++ + Sbjct: 174 GH-----AEHPWRDHMTEIARHPNVVAKISGVVAYAEEDWA-------LDSIRPYVEHTI 221 Query: 232 EAFGPQRLMFGSDWPVCLLAASYA 255 FG R+++GSDWPVC L + + Sbjct: 222 SVFGWDRVVWGSDWPVCTLGGNLS 245 >mlr7156 Non COG Length = 297 Score = 61.2 bits (147), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 80/305 (26%), Positives = 123/305 (40%), Gaps = 45/305 (14%) Query: 3 PPRIDAHQHFWFYQPQAYPWIGAGMELLACDRTPGQLQPLLDAEGLGQSIAVQARAGREE 62 P ID H H YPW+ AG+ L D + +A+ +G + + Sbjct: 18 PVIIDTHLHLIDRSALRYPWL-AGVPQLNRDFSYEDYA--TEAQRVGVERVLHMEVDVDP 74 Query: 63 TGFLLELAR--GNRRIAGVVGWEDLAS-----PHLAANVERWGRDKLV-GFRHQLQDEAD 114 +E AR G R AG + +AS A +ER + V GFR L D Sbjct: 75 ADIEVETARVEGLSRQAGSMLVGAIASCRPEDADFPAYLERQRTNPFVKGFRRVLHVVPD 134 Query: 115 VGGCVDAPAFNAGVAWLQRQGMVYDVLVYARQMAEVQAFCARHDRHWLVLDHLGKPALTE 174 + F + L G+ +D++V Q+ + A VLDH G P + Sbjct: 135 --DLSEGAVFRDNIKRLGGIGLTFDLVVLPHQIPKAIALADLAPDVQFVLDHCGVPDIK- 191 Query: 175 FGRGDTAFEGWQRQLRALAALPHVACKLSGLVTEAD---WQRGLRQKDFDNISLCLDTAL 231 A W+ + +A P+V K+SG+V AD W G + ++ + Sbjct: 192 ----GGAEHPWRAHMSEIARRPNVMAKISGVVAYADPVNWTVG-------TLRPYVEHTI 240 Query: 232 EAFGPQRLMFGSDWPVCLL--------AASYAKVAGVVREWAATRLSAAEQELLWGATAA 283 AFG R+++GSDWPVC L AA++A ++G S AE++ L A Sbjct: 241 AAFGWDRIVWGSDWPVCTLGGGLSTWVAATHALLSGC---------SIAERDRLLSGNAR 291 Query: 284 RCYGL 288 + + L Sbjct: 292 KLWRL 296