BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0493.p (243 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGl1076 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 432 e-121 BMEII0980 Non COG 275 5e-74 SA2266 [R] COG4221 Short-chain alcohol dehydrogenase of unknown ... 106 5e-23 AGpA564 [IQR] COG1028 Dehydrogenases with different specificitie... 105 9e-23 TM1724 [IQR] COG1028 Dehydrogenases with different specificities... 103 2e-22 L179660 [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 100 4e-21 YMR226c [R] COG4221 Short-chain alcohol dehydrogenase of unknown... 100 4e-21 PAB1085 [IQR] COG1028 Dehydrogenases with different specificitie... 97 4e-20 PA0658 [R] COG0300 Short-chain dehydrogenases of various substra... 97 5e-20 BH3896 [IQR] COG1028 Dehydrogenases with different specificities... 96 7e-20 >AGl1076 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 243 Score = 432 bits (1112), Expect = e-121, Method: Compositional matrix adjust. Identities = 207/243 (85%), Positives = 222/243 (91%) Query: 1 MTQSLAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARLGPQAIPQVT 60 M++SL GKIAVITGAASGIGLATTE LL G TVVMVDWN KAL++LAA+LG +AIPQVT Sbjct: 1 MSESLQGKIAVITGAASGIGLATTEALLEQGATVVMVDWNEKALNDLAAKLGERAIPQVT 60 Query: 61 NLLDAESCAAMAPEILKKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVMKNV 120 NLLDA+SC AM PEIL KVDHIDILYCNAGTYIGG+L +TTPEAIDKMLNLNVNAVMKNV Sbjct: 61 NLLDADSCNAMIPEILGKVDHIDILYCNAGTYIGGDLTETTPEAIDKMLNLNVNAVMKNV 120 Query: 121 HAVAPHMMQRKSGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQ 180 AV PHM +RK+GDIIVTCS+AGHFPTYWEPVY+GSKWAIT FVQ MRRQMIPHGVRV Q Sbjct: 121 QAVVPHMSERKTGDIIVTCSIAGHFPTYWEPVYSGSKWAITSFVQGMRRQMIPHGVRVAQ 180 Query: 181 VSPGPVISALLADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNVTIRDMLVLPTNF 240 VSPGPV+SALLADWPEENLRKAKESGSLI+ SEVADAV YMLTR R VTIRDMLVLPTNF Sbjct: 181 VSPGPVVSALLADWPEENLRKAKESGSLIDASEVADAVVYMLTRKRTVTIRDMLVLPTNF 240 Query: 241 DRV 243 DRV Sbjct: 241 DRV 243 >BMEII0980 Non COG Length = 242 Score = 275 bits (704), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 128/241 (53%), Positives = 174/241 (72%) Query: 1 MTQSLAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARLGPQAIPQVT 60 M Q L G++A +TGAASGIG + ++ G+TV +VD + AL E LG +A P V Sbjct: 1 MAQDLNGRVAAVTGAASGIGFECAKHMIGAGITVFLVDRDENALAEKCVELGDKAKPLVV 60 Query: 61 NLLDAESCAAMAPEILKKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVMKNV 120 +LLD S A M P+IL++ +DI + NAG+YIGG L + P+A D+MLNLN+NA + V Sbjct: 61 DLLDPASVATMMPQILEQAGQLDIFHANAGSYIGGPLWEGDPDAWDRMLNLNINAAFRTV 120 Query: 121 HAVAPHMMQRKSGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQ 180 HAV PHMM+RK+GDII+T SVAG P WEP+Y SK A+ FV T+RRQ+ PHG+RVG Sbjct: 121 HAVLPHMMERKTGDIIMTSSVAGVVPVIWEPIYTASKHAVQAFVHTVRRQVAPHGIRVGG 180 Query: 181 VSPGPVISALLADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNVTIRDMLVLPTNF 240 V PGPV++AL++DWP+ L +A +GSL+EP EVA++V +MLTR RN+TIRD+++LP + Sbjct: 181 VLPGPVVTALISDWPQAKLDEALAAGSLMEPKEVAESVMFMLTRPRNITIRDLVILPQSL 240 Query: 241 D 241 D Sbjct: 241 D 241 >SA2266 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Sau Staphylococcus aureus N315 Length = 231 Score = 106 bits (264), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 65/234 (27%), Positives = 120/234 (51%), Gaps = 8/234 (3%) Query: 5 LAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARLGPQAIPQV-TNLL 63 L KIAV+TGA SGIG A L G VV+ N L +A +L ++ V T++ Sbjct: 4 LTDKIAVVTGAGSGIGEAIATLLHEEGAKVVLAGRNKDKLQNVANQLAQDSVKVVPTDVT 63 Query: 64 DAESCAAMAPEILKKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVMKNVHAV 123 + E + + +DI+ +AG + ++ D + D M+++N+ + A Sbjct: 64 NKEEVDELMKIAQQTFGGLDIVINSAGQMLSSKITDYQVDEWDSMIDVNIKGTLYTAQAA 123 Query: 124 APHMMQRKSGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQVSP 183 P M+++ SG +I S++G T +Y+ +K A+ Q + +++ GV+V +SP Sbjct: 124 LPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAVHTITQGLEKELAKTGVKVTSISP 183 Query: 184 GPVISALLADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNVTIRDMLVLP 237 G V +A+ A + + +K ++P ++A+AV Y LT+ ++V + ++ V P Sbjct: 184 GMVDTAITAAYNPSDRKK-------LDPQDIAEAVLYALTQPKHVNVNEITVRP 230 >AGpA564 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 286 Score = 105 bits (262), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 70/235 (29%), Positives = 113/235 (48%), Gaps = 11/235 (4%) Query: 5 LAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARLGPQAIPQVTNLLD 64 L K A+ITG SGIGL T + ++ G V + N + LD LGP A+ + D Sbjct: 41 LKSKRALITGGTSGIGLETARQFISEGARVAVTGTNPETLDAARRELGPSALVIKADAAD 100 Query: 65 AESCAAMAPEILKKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVMKNVHAVA 124 + +A I + +D+++ NAG + L + D++ N+NV + A+ Sbjct: 101 VDGQKVVAETIREAFGGLDVVFINAGVALLKPLEAWDEKGFDRVFNINVKGPYFLLQALL 160 Query: 125 PHMMQRKSGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQVSPG 184 P + S I++ SV H VYA SK A+ F++T+ ++I G+R+ VSPG Sbjct: 161 PILANPAS--IVLNTSVNAHIGMPNSSVYAASKAALQSFIRTLSGELIARGIRLNAVSPG 218 Query: 185 PVISALLAD--WPEENLRKAKES-------GSLIEPSEVADAVEYMLTRNRNVTI 230 P+ + L + E +L+ ES G EPSE+A AV ++ + T+ Sbjct: 219 PISTPLYGKLGFTETDLKNVAESIQNLVPAGRFGEPSEIAKAVVFLASDEAAYTV 273 >TM1724 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Tma Thermotoga maritima Length = 246 Score = 103 bits (258), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 70/227 (30%), Positives = 113/227 (49%), Gaps = 14/227 (6%) Query: 5 LAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDEL---AARLGPQAIPQVTN 61 L GK+ +ITGAASGIG ATT G TV+ D + + LD L A L + P V N Sbjct: 3 LEGKVCLITGAASGIGKATTLLFAQEGATVIAGDISKENLDSLVKEAEGLPGKVDPYVLN 62 Query: 62 LLDAESCAAMAPEILKKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVMKNVH 121 + D + + ++++K ID+L NAG LV E D ++N+N+ V Sbjct: 63 VTDRDQIKEVVEKVVQKYGRIDVLVNNAGITRDALLVRMKEEDWDAVINVNLKGVFNVTQ 122 Query: 122 AVAPHMMQRKSGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQV 181 V P+M+++++G I+ SV G + + YA SK + +T +++ +RV V Sbjct: 123 MVVPYMIKQRNGSIVNVSSVVGIYGNPGQTNYAASKAGVIGMTKTWAKELAGRNIRVNAV 182 Query: 182 SPGPVISALLADWPEENLRKAKES-------GSLIEPSEVADAVEYM 221 +PG + + + PE KA+E+ G +P EVA + ++ Sbjct: 183 APGFIETPMTEKLPE----KARETALSRIPLGRFGKPEEVAQVILFL 225 >L179660 [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Lla Lactococcus lactis Length = 250 Score = 100 bits (248), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 73/248 (29%), Positives = 117/248 (47%), Gaps = 12/248 (4%) Query: 1 MTQSLAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARL---GPQAIP 57 M+ ++ K+ +ITGA+SGIG AT E L G +V+ L ELA ++ G QAI Sbjct: 1 MSNNIENKVVLITGASSGIGQATAELLAKKGAKIVLAARRENRLQELADKIKKAGGQAIY 60 Query: 58 QVTNLLDAESCAAMAPEILKKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVM 117 QVT++ + E + + ID ++ NAG L E + M+++N+ V+ Sbjct: 61 QVTDVTNPEESKKLVQYAKENFGKIDAIFLNAGIMPSSPLSALHVEEWENMVDINLKGVL 120 Query: 118 KNVHAVAPHMMQRKSGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHG-- 175 + AV P +KSG +I T SVAG VY +KWA+ ++ +R + G Sbjct: 121 NGLAAVLPEFTAQKSGHVITTSSVAGLKAYPNGGVYGATKWAVRELMEVLRMESAQEGTH 180 Query: 176 VRVGQVSPGPVISALL-----ADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNVTI 230 +R + P + + LL D E ++ G I P +A V + L + +V + Sbjct: 181 IRTATIYPAAINTELLNTITDKDAAEGMTALYEQYG--ISPEAIARIVAFALEQPEDVNV 238 Query: 231 RDMLVLPT 238 + V PT Sbjct: 239 SEFTVGPT 246 >YMR226c [R] COG4221 Short-chain alcohol dehydrogenase of unknown specificity Sce Saccharomyces cerevisiae Length = 267 Score = 100 bits (248), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 77/251 (30%), Positives = 124/251 (49%), Gaps = 16/251 (6%) Query: 3 QSLAGKIAVITGAASGIGLATTEKLL--ANG-VTVVMVDWNAKALDELAARLG---PQAI 56 + LA K +ITGA++GIG AT + L +NG + +++ + L+EL + P A Sbjct: 9 ERLAKKTVLITGASAGIGKATALEYLEASNGDMKLILAARRLEKLEELKKTIDQEFPNAK 68 Query: 57 PQVTNL--LDAESCAAMAPEILKKVDHIDILYCNAGTYIGGELV-DTTPEAIDKMLNLNV 113 V L AE + ++ IDIL NAG +G + V E I + + NV Sbjct: 69 VHVAQLDITQAEKIKPFIENLPQEFKDIDILVNNAGKALGSDRVGQIATEDIQDVFDTNV 128 Query: 114 NAVMKNVHAVAPHMMQRKSGDIIVTCSVAGH--FPTYWEPVYAGSKWAITCFVQTMRRQM 171 A++ AV P + SGDI+ S+AG +PT +Y SK+A+ F ++R+++ Sbjct: 129 TALINITQAVLPIFQAKNSGDIVNLGSIAGRDAYPT--GSIYCASKFAVGAFTDSLRKEL 186 Query: 172 IPHGVRVGQVSPGPV---ISALLADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNV 228 I +RV ++PG V S + EE + + + + +VAD + Y +R +N Sbjct: 187 INTKIRVILIAPGLVETEFSLVRYRGNEEQAKNVYKDTTPLMADDVADLIVYATSRKQNT 246 Query: 229 TIRDMLVLPTN 239 I D L+ PTN Sbjct: 247 VIADTLIFPTN 257 >PAB1085 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Pab Pyrococcus abyssi Length = 240 Score = 97.1 bits (240), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 73/226 (32%), Positives = 115/226 (50%), Gaps = 13/226 (5%) Query: 4 SLAGKIAVITGAASGIGLATTEKLLANGVTVVMV----DWNAKALDELAARLGPQAIPQV 59 L GK+A+ITGA+ GIG A +L GV VV+ + AK +EL + G + + Sbjct: 2 ELKGKVALITGASRGIGRAIAIELAKRGVNVVINYRSNEEEAKKTEELCRQYGVETLLVK 61 Query: 60 TNLLDAESCAAMAPEILKKVDHIDILYCNAGTYIGGELVD---TTPEAIDKMLNLNVNAV 116 ++ + E M +++ K IDIL NAG I G+ D T E D+++++N+ Sbjct: 62 ADVSNREEVREMVKKVIDKFGRIDILINNAG--ILGKTKDPLEVTDEEWDRVISVNLKGA 119 Query: 117 MKNVHAVAPHMMQRKSGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGV 176 V +M K G I+ S+AG P YA SK + + R + P+ + Sbjct: 120 FIVTQEVLRYM---KKGKIVNIASIAGKDGGTVGPHYAASKGGLIALTFNLARHLAPN-I 175 Query: 177 RVGQVSPGPVISALLADWPEENLRKAKESGSLIEPSEVADAVEYML 222 V V+PGPV + +L+ +E L+K +G + +PSEVA AV ++L Sbjct: 176 LVNAVAPGPVDTDMLSSEMKEMLKKLSLTGDIAKPSEVAHAVIFLL 221 >PA0658 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Pae Pseudomonas aeruginosa Length = 266 Score = 96.7 bits (239), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 65/227 (28%), Positives = 108/227 (47%), Gaps = 5/227 (2%) Query: 8 KIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALD----ELAARLGPQAIPQVTNLL 63 + A+ITGA+SGIGLA E L G +++V AL+ ELA R G + + + +L Sbjct: 3 RYALITGASSGIGLALAEALARRGRALILVARQRDALESIAYELAQRFGVEVLFRTCDLS 62 Query: 64 DAESCAAMAPEILKKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVMKNVHAV 123 + + + E+ + ID+L NAG G +D +++ LN+ A+ + H++ Sbjct: 63 EPLQLSGLLHELEQGERQIDLLVNNAGIGTSGPFIDQEWAREQELIELNILALARLCHSL 122 Query: 124 APHMMQRKSGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQVSP 183 M + G I+ SVA P W Y SK + F + +R ++ GV+V + P Sbjct: 123 GARMAAQGGGQILNIASVAAFQPGAWMSNYYASKAYVLHFSEGLREELKERGVKVSVLCP 182 Query: 184 GPVISALLADWPEENLRKAKESGSLIEPSEVADAVEYMLTRNRNVTI 230 GP +A + L + S ++ P EVA L +NR + + Sbjct: 183 GPTRTAFFRE-AGMRLGRLDRSKLMMTPEEVALLALRGLDKNRALIV 228 >BH3896 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bha Bacillus halodurans Length = 246 Score = 95.9 bits (237), Expect = 7e-20, Method: Compositional matrix adjust. Identities = 54/207 (26%), Positives = 106/207 (51%), Gaps = 3/207 (1%) Query: 5 LAGKIAVITGAASGIGLATTEKLLANGVTVVMVDWNAKALDELAARL---GPQAIPQVTN 61 L GK+A+ITGA GIG AT +K G V++ D + + + A + G +A+ V + Sbjct: 3 LNGKVAMITGAGRGIGAATAKKFAREGAKVIVCDVREEEVAKTVAEIQDGGGEALGSVVD 62 Query: 62 LLDAESCAAMAPEILKKVDHIDILYCNAGTYIGGELVDTTPEAIDKMLNLNVNAVMKNVH 121 + + + +++++ + +D++ NAG +L + T D ++++N+ V Sbjct: 63 VTQRKDVKNVINQVIERFETLDVVVNNAGITADAQLTNMTDAQWDDVIDVNLKGVFIVTQ 122 Query: 122 AVAPHMMQRKSGDIIVTCSVAGHFPTYWEPVYAGSKWAITCFVQTMRRQMIPHGVRVGQV 181 V M ++K G I+ SV G + + + YA SKW + +T +++ + +RV V Sbjct: 123 EVTTIMKEQKRGVILNASSVVGSYGNFGQTNYAASKWGVNGMTKTWAKELGRYNIRVNAV 182 Query: 182 SPGPVISALLADWPEENLRKAKESGSL 208 +PG +++ + PE+ L+ +E L Sbjct: 183 APGFILTPMTEKMPEKVLKVMEEKAVL 209