BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0430.p (215 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0384 [O] COG0625 Glutathione S-transferase Rso Ralstonia sola... 244 1e-64 VC1347 [O] COG0625 Glutathione S-transferase Vch Vibrio cholerae 218 6e-57 mlr1135 [O] COG0625 Glutathione S-transferase Mlo Mesorhizobium ... 214 1e-55 Z3391 [O] COG0625 Glutathione S-transferase EcZ Escherichia coli... 198 6e-51 ECs3028 [O] COG0625 Glutathione S-transferase Ecs Escherichia co... 198 6e-51 PA2007 [O] COG0625 Glutathione S-transferase Pae Pseudomonas aer... 192 5e-49 STM2176 [O] COG0625 Glutathione S-transferase Sty Salmonella typ... 192 6e-49 PA2473 [O] COG0625 Glutathione S-transferase Pae Pseudomonas aer... 190 2e-48 RSc1085 [O] COG0625 Glutathione S-transferase Rso Ralstonia sola... 188 1e-47 SMc03206 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium... 180 2e-45 >RSc0384 [O] COG0625 Glutathione S-transferase Rso Ralstonia solanacearum Length = 216 Score = 244 bits (622), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 119/202 (58%), Positives = 146/202 (72%), Gaps = 2/202 (0%) Query: 4 LYSYFRSSASYRVRIALHLKGLPYETVPVHLLNQGGEQLLPAFTEINPHALVPVLAEEGH 63 LY+YFRSSAS+RVRIAL +KGLPY+ PVHLL GEQ P F ++NP ALVPVL + Sbjct: 5 LYNYFRSSASFRVRIALEIKGLPYDYAPVHLLK--GEQAAPDFVKLNPEALVPVLCDGTE 62 Query: 64 YVSQSLAMLELLEERHPTPSLLPGDAFQRAHIRALSLAIACDIHPLNNLRVLKYLKRELG 123 ++QSLA++E LEE HP P+LLPG A RAHIRA++LAIAC+IHPLNN RVLKYLK Sbjct: 63 VLNQSLAIVEYLEETHPEPTLLPGSAADRAHIRAIALAIACEIHPLNNPRVLKYLKHTFN 122 Query: 124 MDDVRKNAWIAHWINLGFTALERQLAADTTRGHFCVGDAPTMADCCLVPQIFNARRFEVD 183 + D +N W HW+ LGF ALE +L G CVGD PT+AD CLVPQ+FN +RF+V Sbjct: 123 VGDDARNDWYRHWVRLGFAALETRLTQSPRTGACCVGDTPTLADLCLVPQVFNGKRFDVA 182 Query: 184 MAPYPTLCAIEQACHALPAFQQ 205 + YPTL + + C A PAFQ+ Sbjct: 183 VEDYPTLARVFEHCMAQPAFQR 204 >VC1347 [O] COG0625 Glutathione S-transferase Vch Vibrio cholerae Length = 215 Score = 218 bits (556), Expect = 6e-57, Method: Compositional matrix adjust. Identities = 108/211 (51%), Positives = 144/211 (68%), Gaps = 2/211 (0%) Query: 4 LYSYFRSSASYRVRIALHLKGLPYETVPVHLLNQGGEQLLPAFTEINPHALVPVLAEEGH 63 LY Y+RSSA+YRVRIAL++K L YE+ VHL +GGEQ F +NP L+PVL + Sbjct: 6 LYGYWRSSAAYRVRIALNIKQLVYESRAVHLSREGGEQHHAEFHRLNPSELIPVLIDGEL 65 Query: 64 YVSQSLAMLELLEERHPTPSLLPGDAFQRAHIRALSLAIACDIHPLNNLRVLKYLKRELG 123 ++QSLA++E L+E +P P L+P +R ++AL+L IA DIHP+NNLR+L+YL +LG Sbjct: 66 CLNQSLAIIEYLDETYPAPRLIPERGAERYQVKALALDIAADIHPINNLRILQYLTAKLG 125 Query: 124 MDDVRKNAWIAHWINLGFTALERQLAADTTRGHFCVGDAPTMADCCLVPQIFNARRFEVD 183 + D KN W HWI+ GF LE +L T G +CVG+ ++ D CLVPQ++NA RF++D Sbjct: 126 VADEEKNRWYRHWIDKGFQGLEEKLR--HTAGEYCVGNRLSLVDVCLVPQVYNAERFDLD 183 Query: 184 MAPYPTLCAIEQACHALPAFQQAHPAQQPDA 214 M+ YPTL I ALPAF QA P QPDA Sbjct: 184 MSRYPTLQQIAARLRALPAFAQAAPENQPDA 214 >mlr1135 [O] COG0625 Glutathione S-transferase Mlo Mesorhizobium loti Length = 216 Score = 214 bits (544), Expect = 1e-55, Method: Compositional matrix adjust. Identities = 110/213 (51%), Positives = 138/213 (64%), Gaps = 3/213 (1%) Query: 3 TLYSYFRSSASYRVRIALHLKGLPYETVPVHLLNQGGEQLLPAFTEINPHALVPVLA-EE 61 L++Y+RSS SYRVRIAL +KGL Y+ VP HL + GE L PA+ +NP LVP L ++ Sbjct: 5 VLHNYYRSSTSYRVRIALEMKGLSYDYVPHHL--RHGEHLEPAYLAVNPQGLVPALVLDD 62 Query: 62 GHYVSQSLAMLELLEERHPTPSLLPGDAFQRAHIRALSLAIACDIHPLNNLRVLKYLKRE 121 G ++QSLA++E L+E HP P LLP D RA +R L+ IACDIHP+NNLRVL L+ Sbjct: 63 GTLLTQSLAIIEYLDEIHPAPPLLPKDPLGRARVRMLAQMIACDIHPVNNLRVLTSLRTL 122 Query: 122 LGMDDVRKNAWIAHWINLGFTALERQLAADTTRGHFCVGDAPTMADCCLVPQIFNARRFE 181 G D W HW+N GF LE+ LA+ G FC G+A +AD CL QI + RF Sbjct: 123 FGAGDQDITNWFRHWVNEGFQPLEKILASSPQTGTFCHGEAAGLADICLAAQITSNARFG 182 Query: 182 VDMAPYPTLCAIEQACHALPAFQQAHPAQQPDA 214 VD+ PYPT+ I AC ALPAFQ+A P Q DA Sbjct: 183 VDLTPYPTITRINAACMALPAFQKAAPQNQMDA 215 >Z3391 [O] COG0625 Glutathione S-transferase EcZ Escherichia coli O157:H7 EDL933 Length = 215 Score = 198 bits (504), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 105/214 (49%), Positives = 135/214 (63%), Gaps = 4/214 (1%) Query: 1 MTTLYSYFRSSASYRVRIALHLKGLPYETVPVHLLNQGGEQLLPAFTEINPHALVP-VLA 59 M LYS+F SSASYRVRIAL LKG+ Y+T V++ + G+Q A+ +NP LVP +L Sbjct: 1 MMKLYSFFNSSASYRVRIALALKGINYQTEGVNI--RIGQQNELAYRRMNPVGLVPTLLT 58 Query: 60 EEGHYVSQSLAMLELLEERHPTPSLLPGDAFQRAHIRALSLAIACDIHPLNNLRVLKYLK 119 +EG + QSLA+++ LE +P L+P + R + + AIACDIHPLNNLRVL+YL Sbjct: 59 DEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIACDIHPLNNLRVLRYLT 118 Query: 120 RELGMDDVRKNAWIAHWINLGFTALERQLAADTTRGHFCVGDAPTMADCCLVPQIFNARR 179 EL + + K W AHWI G +A+E QL G FCVG+ PT+ADCCLVPQ NA R Sbjct: 119 EELNVSEEEKKRWYAHWIQQGLSAVE-QLLRQNQSGQFCVGETPTLADCCLVPQWANALR 177 Query: 180 FEVDMAPYPTLCAIEQACHALPAFQQAHPAQQPD 213 D++ YP A+ AC LPAF A P Q D Sbjct: 178 MNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQD 211 >ECs3028 [O] COG0625 Glutathione S-transferase Ecs Escherichia coli O157:H7 Length = 215 Score = 198 bits (504), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 105/214 (49%), Positives = 135/214 (63%), Gaps = 4/214 (1%) Query: 1 MTTLYSYFRSSASYRVRIALHLKGLPYETVPVHLLNQGGEQLLPAFTEINPHALVP-VLA 59 M LYS+F SSASYRVRIAL LKG+ Y+T V++ + G+Q A+ +NP LVP +L Sbjct: 1 MMKLYSFFNSSASYRVRIALALKGINYQTEGVNI--RIGQQNELAYRRMNPVGLVPTLLT 58 Query: 60 EEGHYVSQSLAMLELLEERHPTPSLLPGDAFQRAHIRALSLAIACDIHPLNNLRVLKYLK 119 +EG + QSLA+++ LE +P L+P + R + + AIACDIHPLNNLRVL+YL Sbjct: 59 DEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIACDIHPLNNLRVLRYLT 118 Query: 120 RELGMDDVRKNAWIAHWINLGFTALERQLAADTTRGHFCVGDAPTMADCCLVPQIFNARR 179 EL + + K W AHWI G +A+E QL G FCVG+ PT+ADCCLVPQ NA R Sbjct: 119 EELNVSEEEKKRWYAHWIQQGLSAVE-QLLRQNQSGQFCVGETPTLADCCLVPQWANALR 177 Query: 180 FEVDMAPYPTLCAIEQACHALPAFQQAHPAQQPD 213 D++ YP A+ AC LPAF A P Q D Sbjct: 178 MNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQD 211 >PA2007 [O] COG0625 Glutathione S-transferase Pae Pseudomonas aeruginosa Length = 212 Score = 192 bits (487), Expect = 5e-49, Method: Compositional matrix adjust. Identities = 99/212 (46%), Positives = 135/212 (63%), Gaps = 6/212 (2%) Query: 3 TLYSYFRSSASYRVRIALHLKGLPYETVPVHLLNQGGEQLLPAFTEINPHALVPVL-AEE 61 LY+Y+RS++SYRVRIAL LKGL Y+++PV+L+ GGE PA+ +NP VP L +E Sbjct: 2 KLYTYYRSTSSYRVRIALALKGLDYQSLPVNLIRDGGEHRQPAYLALNPQGRVPALQVDE 61 Query: 62 GHYVSQSLAMLELLEERHPTPSLLPGDAFQRAHIRALSLAIACDIHPLNNLRVLKYLKRE 121 G + QS A++E LEER+P P+LL D +RA R ++ + CDIHPL+N VL L R+ Sbjct: 62 GELLIQSPAIIEYLEERYPQPALLSSDPLRRARERGVAALVGCDIHPLHNASVLNLL-RQ 120 Query: 122 LGMDDVRKNAWIAHWINLGFTALERQLAADTTRGHFCVGDAPTMADCCLVPQIFNARRFE 181 G D+ + WI HW+ G A+E QL D +C GD P +AD LVPQ++ A RF Sbjct: 121 WGHDEEQVRQWIGHWVGQGLAAVE-QLIGDQG---WCFGDRPGLADVYLVPQLYAAERFG 176 Query: 182 VDMAPYPTLCAIEQACHALPAFQQAHPAQQPD 213 V + +P + + A PAF+QAHPA QPD Sbjct: 177 VALDAWPRIRRVADLAAAHPAFRQAHPANQPD 208 >STM2176 [O] COG0625 Glutathione S-transferase Sty Salmonella typhimurium LT2 Length = 214 Score = 192 bits (487), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 102/211 (48%), Positives = 133/211 (63%), Gaps = 4/211 (1%) Query: 4 LYSYFRSSASYRVRIALHLKGLPYETVPVHLLNQGGEQLLPAFTEINPHALVPVL-AEEG 62 LYS+F SSASYRVRIAL LKG+ Y+TV V++ + G+Q A+ +NP LVP L ++G Sbjct: 3 LYSFFNSSASYRVRIALALKGIDYQTVGVNI--RIGQQNALAYRRMNPVGLVPTLVTDDG 60 Query: 63 HYVSQSLAMLELLEERHPTPSLLPGDAFQRAHIRALSLAIACDIHPLNNLRVLKYLKREL 122 + QSLA+++ L+ P LLP R+ + AIACDIHP+NN+RVL+YL EL Sbjct: 61 ESLGQSLAIVDWLDRHFPQTPLLPTSDPARSQALEIVYAIACDIHPINNMRVLRYLTDEL 120 Query: 123 GMDDVRKNAWIAHWINLGFTALERQLAADTTRGHFCVGDAPTMADCCLVPQIFNARRFEV 182 + + K W AHWI G +A+E QL + G FCVGDAP +ADCCL+PQ NA R Sbjct: 121 KVSEEDKKRWYAHWIQQGLSAVE-QLLRKSQSGRFCVGDAPGLADCCLIPQWANALRMGC 179 Query: 183 DMAPYPTLCAIEQACHALPAFQQAHPAQQPD 213 D++ YP A+ AC LPAF A P Q D Sbjct: 180 DLSGYPRCKAVYDACVQLPAFIAAAPENQQD 210 >PA2473 [O] COG0625 Glutathione S-transferase Pae Pseudomonas aeruginosa Length = 214 Score = 190 bits (483), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 108/211 (51%), Positives = 135/211 (63%), Gaps = 4/211 (1%) Query: 4 LYSYFRSSASYRVRIALHLKGLPYETVPVHLLNQGGEQLLPAFTEINPHALVPVLAE-EG 62 LYS+F SS SYRVRIAL LKGL Y+ VPV+L + GEQL PA + NP +P L + +G Sbjct: 3 LYSFFNSSTSYRVRIALALKGLDYQVVPVNL--RQGEQLRPADRQRNPMGALPTLVDADG 60 Query: 63 HYVSQSLAMLELLEERHPTPSLLPGDAFQRAHIRALSLAIACDIHPLNNLRVLKYLKREL 122 SQSLA+++ L+ P P L+P D RA L+L +ACDIHPLNN+RVLKYL + L Sbjct: 61 RRFSQSLAIIDYLDAVQPEPRLIPLDPLHRAQALELALLVACDIHPLNNVRVLKYLTQVL 120 Query: 123 GMDDVRKNAWIAHWINLGFTALERQLAADTTRGHFCVGDAPTMADCCLVPQIFNARRFEV 182 G+D + W AHW+ G A E L RG F G A + +CCLVPQ+ NARR Sbjct: 121 GIDAEDRQRWYAHWVAEGLAAAE-TLLNRHRRGAFFAGAAAGIVECCLVPQLANARRMGC 179 Query: 183 DMAPYPTLCAIEQACHALPAFQQAHPAQQPD 213 D+APYP L +E C AL AFQ+A P +QPD Sbjct: 180 DLAPYPALLELEGRCLALEAFQRASPERQPD 210 >RSc1085 [O] COG0625 Glutathione S-transferase Rso Ralstonia solanacearum Length = 213 Score = 188 bits (477), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 99/210 (47%), Positives = 134/210 (63%), Gaps = 2/210 (0%) Query: 4 LYSYFRSSASYRVRIALHLKGLPYETVPVHLLNQGGEQLLPAFTEINPHALVPVLAEEGH 63 LYS+F SS SYRVRIAL LKGLPY+ +PV++ G + +NP A VP L + Sbjct: 3 LYSFFNSSTSYRVRIALALKGLPYDYLPVNI-RTGEHRAADYVAHVNPSAGVPTLVDGAL 61 Query: 64 YVSQSLAMLELLEERHPTPSLLPGDAFQRAHIRALSLAIACDIHPLNNLRVLKYLKRELG 123 + QSLA+++ L+ RHP P L+P D +RA + L+ AIACDIHP+NNLRVL+YL+ EL Sbjct: 62 RLGQSLAIIDYLDARHPQPRLVPADPLRRARVLELASAIACDIHPVNNLRVLRYLQDELK 121 Query: 124 MDDVRKNAWIAHWINLGFTALERQLAADTTRGHFCVGDAPTMADCCLVPQIFNARRFEVD 183 + +K+AW HWI+ G ++ER L A G +C GDAPT+AD LVPQ+ NA R Sbjct: 122 VTPQQKDAWYRHWIDEGLASVER-LLARHGHGPWCFGDAPTLADVALVPQVANALRMGCP 180 Query: 184 MAPYPTLCAIEQACHALPAFQQAHPAQQPD 213 + + + A+ PAF +A PA+QPD Sbjct: 181 LDRFERVLAVHAHAGTHPAFAEAAPARQPD 210 >SMc03206 [O] COG0625 Glutathione S-transferase Sme Sinorhizobium meliloti Length = 217 Score = 180 bits (457), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 99/207 (47%), Positives = 126/207 (60%), Gaps = 3/207 (1%) Query: 2 TTLYSYFRSSASYRVRIALHLKGLPYETVPVHLLNQGGEQLLPAFTEINPHALVPVLAEE 61 T LY Y+RSSASYRVRIAL+L G Y +VPV LL + P NP LVPVL + Sbjct: 9 TVLYDYWRSSASYRVRIALNLCGEAYRSVPVDLLAKA--HRAPEHLARNPQGLVPVLDID 66 Query: 62 GHYVSQSLAMLELLEERHPTPSLLPGDAFQRAHIRALSLAIACDIHPLNNLRVLKYLKRE 121 G ++QSLA++E L E LLP R +RALS A+A DIHP+ NL V+ + Sbjct: 67 GERLTQSLAIIEYLAETRDGTGLLPAHPIDRQRVRALSYAVAMDIHPVCNLGVVARVMAG 126 Query: 122 LGMDDVRKNAWIAHWINLGFTALERQLAADTTRGHFCVGDAPTMADCCLVPQIFNARRFE 181 G + + W+ +I G A ER L T G FC GD PTMAD CLVPQ++NARR++ Sbjct: 127 AGDGEAARREWMQKFIGEGLAAFERMLDHPAT-GAFCHGDRPTMADLCLVPQVYNARRWD 185 Query: 182 VDMAPYPTLCAIEQACHALPAFQQAHP 208 VD+A P L AI++ C + AFQ+AHP Sbjct: 186 VDLAACPLLVAIDRRCAGIDAFQRAHP 212