BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0368.p (252 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2534 [IQR] COG1028 Dehydrogenases with different specificitie... 359 3e-99 PA2554 [IQR] COG1028 Dehydrogenases with different specificities... 316 3e-86 BMEII0816 [IQR] COG1028 Dehydrogenases with different specificit... 293 2e-79 mlr2803 [IQR] COG1028 Dehydrogenases with different specificitie... 292 6e-79 SMa1452 [IQR] COG1028 Dehydrogenases with different specificitie... 289 4e-78 AGc2613 [IQR] COG1028 Dehydrogenases with different specificitie... 264 2e-70 Rv1144 [IQR] COG1028 Dehydrogenases with different specificities... 235 9e-62 MT1177 [IQR] COG1028 Dehydrogenases with different specificities... 235 9e-62 CC0124 [IQR] COG1028 Dehydrogenases with different specificities... 224 1e-58 Rv1350 [IQR] COG1028 Dehydrogenases with different specificities... 126 4e-29 >RSc2534 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Rso Ralstonia solanacearum Length = 252 Score = 359 bits (922), Expect = 3e-99, Method: Compositional matrix adjust. Identities = 180/252 (71%), Positives = 206/252 (81%) Query: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGVFVRCDVSS 60 M I VFI+TG ASGLGA T R +A AGGK V+ADLN+ AG LA E+GG FVRCDVSS Sbjct: 1 MEIRDQVFIVTGGASGLGAGTTRALAEAGGKVVIADLNEAAGAALAQEIGGRFVRCDVSS 60 Query: 61 EEDAQAVVRAAEALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFNMAR 120 E D QA V+AA +LG+L GLVNCAGIAPA + VGK+GPH + F + +NINL+G+FNM R Sbjct: 61 EADGQAAVQAATSLGALAGLVNCAGIAPASRTVGKAGPHPLDQFARVININLIGTFNMIR 120 Query: 121 FAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSRSG 180 AA AM +A GERGVII+TASVAA+DGQIGQ AYAASKG +VAMTL +AR+LSR G Sbjct: 121 LAATAMTANAPNAGGERGVIINTASVAAFDGQIGQAAYAASKGGVVAMTLAIARDLSRDG 180 Query: 181 IRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLNGET 240 IRVMTIAPGIFETPM+L MP EV+D+LG+ VPFPPRLG+ AEYAQLAR IIEN MLNGET Sbjct: 181 IRVMTIAPGIFETPMLLGMPPEVQDALGKMVPFPPRLGKPAEYAQLARAIIENPMLNGET 240 Query: 241 IRLDGAIRMQPK 252 IRLDGAIRMQPK Sbjct: 241 IRLDGAIRMQPK 252 >PA2554 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Pae Pseudomonas aeruginosa Length = 255 Score = 316 bits (810), Expect = 3e-86, Method: Compositional matrix adjust. Identities = 171/252 (67%), Positives = 198/252 (78%), Gaps = 3/252 (1%) Query: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELG--GVFVRCDV 58 MRIE VF++TG +SGLGAATA+M+ GGK VLAD+N EAG AAELG FVR D+ Sbjct: 1 MRIENRVFLVTGGSSGLGAATAKMLVEQGGKVVLADINAEAGAAKAAELGAQARFVRADI 60 Query: 59 SSEEDA-QAVVRAAEALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFN 117 +SE D QAV A EA G L GL NCAG+APAEK++G++G H + F + ++INLVGSFN Sbjct: 61 ASEADGRQAVAAALEAFGGLHGLANCAGVAPAEKVLGRNGIHALESFRRVIDINLVGSFN 120 Query: 118 MARFAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELS 177 M R AAEAM++ E GERGVI++TASVAA+DGQIGQ AY+ASK +V MTLP+AREL+ Sbjct: 121 MLRLAAEAMSQGQPDEGGERGVIVNTASVAAFDGQIGQAAYSASKSGVVGMTLPIARELA 180 Query: 178 RSGIRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLN 237 R GIRVMTIAPGIFETPMM MPQEVRDSLG +VPFPPRLGR EYA L R IIEN MLN Sbjct: 181 RFGIRVMTIAPGIFETPMMAGMPQEVRDSLGASVPFPPRLGRPDEYAALVRQIIENSMLN 240 Query: 238 GETIRLDGAIRM 249 GE IRLDGAIRM Sbjct: 241 GEVIRLDGAIRM 252 >BMEII0816 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bme Brucella melitensis Length = 255 Score = 293 bits (750), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 159/255 (62%), Positives = 194/255 (76%), Gaps = 3/255 (1%) Query: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGV--FVRCDV 58 M+IE VF+ITGA SGLGAA ++M AG K VL D+N EAGE A LG F R DV Sbjct: 1 MQIENRVFLITGAGSGLGAAVSKMAVEAGAKVVLLDVNAEAGEAGAKALGASARFQRTDV 60 Query: 59 SSEEDAQAVVRAAEALGS-LRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFN 117 +S+ D +A + AA S + LVNCAG+AP EK++G+ G H + F +T++INL+G+FN Sbjct: 61 ASDTDGKAAIAAAIEAFSRIDVLVNCAGVAPGEKVLGREGAHKLETFTRTISINLIGTFN 120 Query: 118 MARFAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELS 177 M R AAEAMA++ + GERGVII+TASVAA+DGQIGQ AY+ASKG + AMTLP+AREL+ Sbjct: 121 MLRLAAEAMAKNEPGQGGERGVIINTASVAAFDGQIGQAAYSASKGGVAAMTLPVARELA 180 Query: 178 RSGIRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLN 237 R GIRVMTIAPGIF+TPMM MPQEV+D+LG +VPFPPRLG AEYA L R I+EN MLN Sbjct: 181 RHGIRVMTIAPGIFKTPMMAGMPQEVQDALGASVPFPPRLGEPAEYAALVRHIVENQMLN 240 Query: 238 GETIRLDGAIRMQPK 252 GE IRLDGA+RM K Sbjct: 241 GEVIRLDGALRMAAK 255 >mlr2803 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mlo Mesorhizobium loti Length = 253 Score = 292 bits (747), Expect = 6e-79, Method: Compositional matrix adjust. Identities = 150/253 (59%), Positives = 183/253 (72%), Gaps = 1/253 (0%) Query: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGVFVRCDVSS 60 M G + I+TG SGLG ATAR +AA G + + D+ E +AA++GG+ V+CDVSS Sbjct: 1 MNPNGQIAIVTGGGSGLGEATARALAAKGARVAIFDVGIERAAKVAADIGGISVQCDVSS 60 Query: 61 EEDAQAVV-RAAEALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFNMA 119 + A + A LG R LVNCAGIA K +GK GPH + + K + +NL+G+FNM Sbjct: 61 ADSGTAALAETASKLGEPRILVNCAGIAIGVKTIGKDGPHPLDQYRKVIEVNLIGTFNMI 120 Query: 120 RFAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSRS 179 R A+ A ++ GERGVI++TASVAAYDGQIGQ AY+ASKG +V MTLP+AR+L+RS Sbjct: 121 RLVADRAASLEPLQGGERGVIVNTASVAAYDGQIGQAAYSASKGGVVGMTLPVARDLARS 180 Query: 180 GIRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLNGE 239 GIRV TIAPGIF+TPMM MPQEV+DSLG AVPFP RLG +EYA LA IIEN MLNGE Sbjct: 181 GIRVCTIAPGIFKTPMMAGMPQEVQDSLGAAVPFPSRLGEPSEYAALALHIIENQMLNGE 240 Query: 240 TIRLDGAIRMQPK 252 TIRLDGAIRM PK Sbjct: 241 TIRLDGAIRMAPK 253 >SMa1452 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 255 Score = 289 bits (740), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 150/255 (58%), Positives = 190/255 (74%), Gaps = 3/255 (1%) Query: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGV--FVRCDV 58 M+++ VFI+TGA+SGLGAA RM+A G + DL AGE AAELG F DV Sbjct: 1 MQLKSRVFIVTGASSGLGAAVTRMLAQEGATVLGLDLKPPAGEEPAAELGAAVRFRNADV 60 Query: 59 SSEEDAQAVVRAA-EALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFN 117 ++E DA A + A + G + GLVNCAG AP EK++G+SGPH + F +T+ +NL+G+FN Sbjct: 61 TNEADATAALAFAKQEFGHVHGLVNCAGTAPGEKILGRSGPHALDSFARTVAVNLIGTFN 120 Query: 118 MARFAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELS 177 M R AAE M++ +GERGVI++TAS+AA+DGQIGQ AYAASKG + A+TLP AREL+ Sbjct: 121 MIRLAAEVMSQGEPDADGERGVIVNTASIAAFDGQIGQAAYAASKGGVAALTLPAARELA 180 Query: 178 RSGIRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLN 237 R GIRV+TIAPGIF+TPMM MPQ+V+D+L +VPFPPRLGRA EYA L + I EN MLN Sbjct: 181 RFGIRVVTIAPGIFDTPMMAGMPQDVQDALAASVPFPPRLGRAEEYAALVKHICENTMLN 240 Query: 238 GETIRLDGAIRMQPK 252 GE IRLDGA+RM P+ Sbjct: 241 GEVIRLDGALRMAPR 255 >AGc2613 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 257 Score = 264 bits (674), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 143/258 (55%), Positives = 184/258 (71%), Gaps = 7/258 (2%) Query: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGVFVRCDVSS 60 M IEG ++TGAASGLGAA ARM+AA G + D N EAG+ LAAE+GG V+ DV+S Sbjct: 1 MNIEGAGALVTGAASGLGAAVARMLAARGAAVTIFDRNGEAGKKLAAEIGGKAVQGDVTS 60 Query: 61 EEDAQAVVR-AAEALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFNMA 119 + DAQA ++ AA A G LR LVNCAGI A +++G+ GP + F + + +NL+G+FNM Sbjct: 61 DADAQAAIKVAASAKGGLRILVNCAGIGTAGRILGREGPQPLGDFEQVIRVNLIGTFNMM 120 Query: 120 RFAAEAMARSAAVEEG-----ERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMAR 174 R AA MA +EG + GVI++TASVAA++GQIGQ AYAASKG IV++ LP AR Sbjct: 121 RLAAAHMAEREQ-DEGQDSRQDNGVIVNTASVAAFEGQIGQAAYAASKGGIVSLALPAAR 179 Query: 175 ELSRSGIRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENV 234 EL+R IRV T+APGIF TP++ +PQEV++SL +P P RLG AE+A R +IEN Sbjct: 180 ELARFRIRVNTVAPGIFLTPLLQGLPQEVQESLAGQIPHPSRLGDPAEFADTVRFLIEND 239 Query: 235 MLNGETIRLDGAIRMQPK 252 +NGE IRLDGAIRMQP+ Sbjct: 240 YMNGEVIRLDGAIRMQPR 257 >Rv1144 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mtu Mycobacterium tuberculosis H37Rv Length = 250 Score = 235 bits (599), Expect = 9e-62, Method: Compositional matrix adjust. Identities = 126/254 (49%), Positives = 172/254 (67%), Gaps = 6/254 (2%) Query: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELG--GVFVRCDV 58 M+ + V ++TG ASGLG AT + + AG + V+ DL G+ + LG F + DV Sbjct: 1 MKTKDAVAVVTGGASGLGLATTKRLLDAGAQVVVVDLR---GDDVVGGLGDRARFAQADV 57 Query: 59 SSEEDAQAVVRAAEALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFNM 118 + E + A++LG +R +VNCAG A +++ + G +A F K ++INLVG+FN+ Sbjct: 58 TDEAAVSNALELADSLGPVRVVVNCAGTGNAIRVLSRDGVFPLAAFRKIVDINLVGTFNV 117 Query: 119 ARFAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSR 178 R AE +A++ + E ERGVII+TASVAA+DGQIGQ AY+ASKG +V MTLP+AR+L+ Sbjct: 118 LRLGAERIAKTEPIGE-ERGVIINTASVAAFDGQIGQAAYSASKGGVVGMTLPIARDLAS 176 Query: 179 SGIRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLNG 238 IRV+TIAPG+F+TP++ ++P E + SLGQ VP P RLG EY L IIEN MLNG Sbjct: 177 KLIRVVTIAPGLFDTPLLASLPAEAKASLGQQVPHPSRLGNPDEYGALVLHIIENPMLNG 236 Query: 239 ETIRLDGAIRMQPK 252 E IRLDGAIRM P+ Sbjct: 237 EVIRLDGAIRMAPR 250 >MT1177 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) MtC Mycobacterium tuberculosis CDC1551 Length = 250 Score = 235 bits (599), Expect = 9e-62, Method: Compositional matrix adjust. Identities = 126/254 (49%), Positives = 172/254 (67%), Gaps = 6/254 (2%) Query: 1 MRIEGHVFIITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELG--GVFVRCDV 58 M+ + V ++TG ASGLG AT + + AG + V+ DL G+ + LG F + DV Sbjct: 1 MKTKDAVAVVTGGASGLGLATTKRLLDAGAQVVVVDLR---GDDVVGGLGDRARFAQADV 57 Query: 59 SSEEDAQAVVRAAEALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFNM 118 + E + A++LG +R +VNCAG A +++ + G +A F K ++INLVG+FN+ Sbjct: 58 TDEAAVSNALELADSLGPVRVVVNCAGTGNAIRVLSRDGVFPLAAFRKIVDINLVGTFNV 117 Query: 119 ARFAAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSR 178 R AE +A++ + E ERGVII+TASVAA+DGQIGQ AY+ASKG +V MTLP+AR+L+ Sbjct: 118 LRLGAERIAKTEPIGE-ERGVIINTASVAAFDGQIGQAAYSASKGGVVGMTLPIARDLAS 176 Query: 179 SGIRVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLNG 238 IRV+TIAPG+F+TP++ ++P E + SLGQ VP P RLG EY L IIEN MLNG Sbjct: 177 KLIRVVTIAPGLFDTPLLASLPAEAKASLGQQVPHPSRLGNPDEYGALVLHIIENPMLNG 236 Query: 239 ETIRLDGAIRMQPK 252 E IRLDGAIRM P+ Sbjct: 237 EVIRLDGAIRMAPR 250 >CC0124 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Ccr Caulobacter vibrioides Length = 260 Score = 224 bits (572), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 125/251 (49%), Positives = 162/251 (64%), Gaps = 7/251 (2%) Query: 9 IITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGGVFVRCDVSSEEDAQA-V 67 ++TG ASGLG ATAR +AA G K L D+N+E G +A E+GGVF + +V+S+ D A Sbjct: 10 VVTGGASGLGEATARALAAQGVKVALFDMNEERGLQVAKEIGGVFCKVNVTSDADVDAGF 69 Query: 68 VRAAEALGSLRGLVNCAGIAPA------EKLVGKSGPHTMALFMKTLNINLVGSFNMARF 121 +A A G R LVNCAG A +K G++ + F + + INLVG+F Sbjct: 70 EKARAAHGQERILVNCAGTGNAAKTASRDKATGETKHFPLDAFDRIIQINLVGTFRCIAK 129 Query: 122 AAEAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSRSGI 181 +A+ M +E+GERG I++TASVAA DGQ+GQ AY+ASKG +V MTLP+AR+L GI Sbjct: 130 SAKGMLDLEPLEDGERGAIVNTASVAAEDGQMGQAAYSASKGGVVGMTLPIARDLMGEGI 189 Query: 182 RVMTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLARTIIENVMLNGETI 241 RV TI PGIF TP+M P+ V+ L +VPFP RLG EYAQLA T+I NGE + Sbjct: 190 RVNTILPGIFNTPLMNNAPEAVKAGLAASVPFPKRLGHPEEYAQLALTMITCGYFNGEDV 249 Query: 242 RLDGAIRMQPK 252 RLDG IRM P+ Sbjct: 250 RLDGGIRMAPR 260 >Rv1350 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mtu Mycobacterium tuberculosis H37Rv Length = 247 Score = 126 bits (317), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 87/224 (38%), Positives = 121/224 (54%), Gaps = 19/224 (8%) Query: 9 IITGAASGLGAATARMIAAAGGKPVLADLNQEAGEPLAAELGG----VFVRCDVSSEEDA 64 +ITG A GLG A + A G + VL D+N EA E A LGG + VRCDV+ +D Sbjct: 11 VITGGAQGLGLAIGQRFVAEGARVVLGDVNLEATEVAAKRLGGDDVALAVRCDVTQADDV 70 Query: 65 QAVVRAA-EALGSLRGLVNCAGIAPAEKLVGKSGPHTMALFMKTLNINLVGSFNMARFAA 123 ++R A E G L +VN AGI + T F + + ++L G++N R AA Sbjct: 71 DILIRTAVERFGGLDVMVNNAGITRDATMR----TMTEEQFDQVIAVHLKGTWNGTRLAA 126 Query: 124 EAMARSAAVEEGERGVIIHTASVAAYDGQIGQTAYAASKGAIVAMTLPMARELSRSGIRV 183 M E +RG I++ +SV+ G +GQT Y+A+K IV MT A+EL+ GIRV Sbjct: 127 AIM------RERKRGAIVNMSSVSGKVGMVGQTNYSAAKAGIVGMTKAAAKELAHLGIRV 180 Query: 184 MTIAPGIFETPMMLAMPQEVRDSLGQAVPFPPRLGRAAEYAQLA 227 IAPG+ + M AMPQ + D VP +GRA E +++A Sbjct: 181 NAIAPGLIRSAMTEAMPQRIWDQKLAEVP----MGRAGEPSEVA 220