BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0164.p (287 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp1111 [I] COG0657 Esterase/lipase Rso Ralstonia solanacearum 202 5e-52 SSO2521 [I] COG0657 Esterase/lipase Sso Sulfolobus solfataricus 152 1e-36 BH2248 [I] COG0657 Esterase/lipase Bha Bacillus halodurans 148 2e-35 TVN0727 [I] COG0657 Esterase/lipase Tvo Thermoplasma volcanium 147 2e-35 lin2194 [I] COG0657 Esterase/lipase Lin Listeria innocua 147 3e-35 AF1716 [I] COG0657 Esterase/lipase Afu Archaeoglobus fulgidus 142 6e-34 Rv1400c [I] COG0657 Esterase/lipase Mtu Mycobacterium tuberculos... 135 1e-31 MT1444 [I] COG0657 Esterase/lipase MtC Mycobacterium tuberculosi... 134 2e-31 Rv1399c [I] COG0657 Esterase/lipase Mtu Mycobacterium tuberculos... 129 7e-30 MT1443 [I] COG0657 Esterase/lipase MtC Mycobacterium tuberculosi... 129 7e-30 >RSp1111 [I] COG0657 Esterase/lipase Rso Ralstonia solanacearum Length = 357 Score = 202 bits (515), Expect = 5e-52, Method: Compositional matrix adjust. Identities = 125/254 (49%), Positives = 163/254 (64%), Gaps = 3/254 (1%) Query: 11 DFFVPGPLGRIPVRLYLPATPLARGQ---KRPLVMYFHGGGFVSGSLEDADTPARFIAEH 67 D++ G RI +R++ P G ++P V+Y HGGGFV G ++DAD AR +A Sbjct: 74 DYWTQGYASRIRLRVFRPERRADDGAVPLRQPGVLYLHGGGFVGGCIDDADVSARHLAAT 133 Query: 68 GQLAVLSVGYALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAAS 127 V++VGY+LAPA PFPAAPED +AA ++A +D R+ VAG DAGG L+A+ Sbjct: 134 LPAVVVTVGYSLAPAAPFPAAPEDVYAALCCMAEHAATWGIDRRRLAVAGHDAGGNLSAA 193 Query: 128 LTLMTRDRCGKPLAAQVLISPMLDPSMRLLGDAARLQSDLTAETCAASYRQYLPQTMQRL 187 L ++ RDR G L AQVLI+PMLDP+M + +D +AE CAA YRQYLP+ +R+ Sbjct: 194 LAMIARDRGGPGLRAQVLIAPMLDPTMARVRPDRLADADNSAEACAACYRQYLPRPTERV 253 Query: 188 HPYAAPLEVSRLAGLPPAFIATAECDVLCPEAEKYASSLIRAGVPTQVSRFAGITHAALR 247 HPYAAP+E RL GLPPA + TA D+L EAE+YAS+LI AGV TQV R A H ++ Sbjct: 254 HPYAAPVESRRLHGLPPALLLTAPQDLLRTEAERYASALIEAGVATQVLRVADACHDSIA 313 Query: 248 THLPLLTEIVNFLR 261 T L EI FLR Sbjct: 314 TDRAALDEITCFLR 327 >SSO2521 [I] COG0657 Esterase/lipase Sso Sulfolobus solfataricus Length = 311 Score = 152 bits (383), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 87/242 (35%), Positives = 126/242 (52%), Gaps = 14/242 (5%) Query: 8 DIRDFFVPGPLGRIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTPARFIAEH 67 +IRD +P G I VR+Y P + + P V+Y+HGGGFV G+L+ D+ R I++ Sbjct: 49 NIRDMTIPVRNGDIKVRVYFPN----QRENLPAVVYYHGGGFVYGNLDTHDSVCRLISKL 104 Query: 68 GQLAVLSVGYALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAAS 127 ++SV Y LAP FP DA+ W N +L +D ++ VAGD AGG L+ Sbjct: 105 SNTIIVSVDYRLAPEHKFPTQVYDAYDVVKWLANNGGKLSIDTSKIAVAGDSAGGNLSTV 164 Query: 128 LTLMTRDRCGKPLAAQVLISP---MLD--PSMRLLGDAARLQSDLTAETCAASYRQYLPQ 182 ++++ RD + Q++I P MLD PSM GD LT E +QY+ + Sbjct: 165 VSILDRDNGENVVKYQIMIYPVVNMLDSSPSMYNYGDGYF----LTYERILWYNKQYVKE 220 Query: 183 TMQRLHPYAAPLEVSRLAGLPPAFIATAECDVLCPEAEKYASSLIRAGVPTQVSRFAGIT 242 +P A+P+ ++ LPPA I TAE D L + E YA L +GV T R+ G+ Sbjct: 221 DSDYYNPLASPI-LAESHNLPPALIITAEYDPLRDQGEMYAHKLKVSGVKTISLRYNGMI 279 Query: 243 HA 244 H Sbjct: 280 HG 281 >BH2248 [I] COG0657 Esterase/lipase Bha Bacillus halodurans Length = 385 Score = 148 bits (373), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 89/231 (38%), Positives = 124/231 (53%), Gaps = 14/231 (6%) Query: 21 IPVRLYLPATPLARGQKRPLVMYFHGGGFVSG--SLEDADTPARFIAEHGQLAVLSVGYA 78 IP R+Y P A G P+++Y+HGG F+ G + D R +A V++VGY Sbjct: 88 IPARVYQPK---ASG-PHPIILYYHGGAFLEGYGDINTHDNIVRALAVRTGSIVVAVGYR 143 Query: 79 LAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAASLTLMTRDRCGK 138 +AP PFP A ED++ A W KNA D + V GD AGG +A ++LM RDR G Sbjct: 144 VAPGHPFPTAIEDSYDALDWISKNAELFNGDPANVAVVGDSAGGNIATVVSLMARDREGP 203 Query: 139 PLAAQVLISPMLDPSMRLLGDAARLQSD-----LTAETCAASYRQYLPQTMQRLHPYAAP 193 ++AQVL+ P+ + + + +R D L+ + +Y PQ L+PY +P Sbjct: 204 EISAQVLLYPLT--TFQDVAFPSRDHYDSGYYLLSRAVMVQAREKYTPQQESWLNPYTSP 261 Query: 194 LEVSRLAGLPPAFIATAECDVLCPEAEKYASSLIRAGVPTQVSRFAGITHA 244 L + L G+PPAF+ TAE D L E E YA SL AGVP Q R+ G+ H Sbjct: 262 LN-ADLEGVPPAFVVTAEFDPLRDEGEMYAQSLADAGVPVQAIRYNGVMHG 311 >TVN0727 [I] COG0657 Esterase/lipase Tvo Thermoplasma volcanium Length = 305 Score = 147 bits (372), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 91/243 (37%), Positives = 123/243 (50%), Gaps = 8/243 (3%) Query: 9 IRDFFVPGPLGRIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTPARFIAEHG 68 + D + ++P RLY LV+YFHGGGFV GS+E D+ R IA++ Sbjct: 49 VEDMILDTDAAKLPARLY------DDSNSDSLVLYFHGGGFVFGSIETHDSLCRRIAKYS 102 Query: 69 QLAVLSVGYALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAASL 128 V+SVGY L P FP A DAF + + K + EL VD D++ + GD AGG LAA Sbjct: 103 GSKVISVGYRLGPENKFPVAQIDAFNSYLYVLKRSEELGVDSDKIALMGDSAGGNLAAVA 162 Query: 129 TLMTRDRCGKPLAAQVLISPMLDPSMRLLGDAARLQSDLTAETCAASYRQYLPQTMQRLH 188 +LM RD+ K QVL+ P P M LT E QYL L+ Sbjct: 163 SLMARDKGIKLPRLQVLVYPSTGPDMTSKSYLEYSSIYLTKELLNWFSSQYLNSIEDALN 222 Query: 189 PYAAPLEVSRLAGLPPAFIATAECDVLCPEAEKYASSLIRAGVPTQVSRFAGITHAALRT 248 PY +P+ ++ L+GLP A + T E D L + E Y+++L +GVP R G+ H L Sbjct: 223 PYFSPI-LADLSGLPEAIVVTDEYDPLLEQGETYSATLRDSGVPVTSIRVNGMIHGFLSN 281 Query: 249 -HL 250 HL Sbjct: 282 FHL 284 >lin2194 [I] COG0657 Esterase/lipase Lin Listeria innocua Length = 347 Score = 147 bits (371), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 94/253 (37%), Positives = 132/253 (52%), Gaps = 21/253 (8%) Query: 8 DIRDFFVPGPLGRIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTPARFIAEH 67 D+ + + GP G+IP+R+Y TP G +++Y+HGGGFV G L+ D AR + + Sbjct: 85 DVENKKIDGPAGKIPIRIY---TPKEDGPFE-IIVYYHGGGFVLGGLQTHDAIARKLVQT 140 Query: 68 GQLAVLSVGYALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAAS 127 V++V Y LAP PFPAA EDA+AA W + T L+ ++VAGD GG LA Sbjct: 141 TGARVVTVDYRLAPENPFPAAVEDAYAALLWVQNHRTSLRAKSSDIIVAGDSVGGNLATV 200 Query: 128 LTLMTRDRCGKP-LAAQVLISPMLD----------PSMRLLGDAARLQSDLTAETCAASY 176 +T + + + GKP + AQ+L+ P D PSM + LT E+ + Sbjct: 201 VTQIAKAK-GKPNVTAQILLYPATDIFSRDASVLYPSMDEFAEGYV----LTKESLDKFF 255 Query: 177 RQYLPQTMQRLH-PYAAPLEVSRLAGLPPAFIATAECDVLCPEAEKYASSLIRAGVPTQV 235 + Y+ + R + P AP+ LAGLP FIATAE D L + E YA L AGV Sbjct: 256 KLYIANSSDRKYDPLIAPIRSKDLAGLPKTFIATAEFDPLRDQGEAYAKKLKDAGVEVFA 315 Query: 236 SRFAGITHAALRT 248 RF + H + T Sbjct: 316 KRFEKVPHGFMTT 328 >AF1716 [I] COG0657 Esterase/lipase Afu Archaeoglobus fulgidus Length = 311 Score = 142 bits (359), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 87/250 (34%), Positives = 130/250 (52%), Gaps = 16/250 (6%) Query: 9 IRDFFVPGPLGRIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTPARFIAEHG 68 + D + G G I VR+Y + P+++Y+HGGGFV S+E D R IA Sbjct: 56 VEDRTIKGRNGDIRVRVY------QQKPDSPVLVYYHGGGFVICSIESHDALCRRIARLS 109 Query: 69 QLAVLSVGYALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAASL 128 V+SV Y LAP FPAA D + A W +NA EL++D ++ V GD AGG LAA++ Sbjct: 110 NSTVVSVDYRLAPEHKFPAAVYDCYDATKWVAENAEELRIDPSKIFVGGDSAGGNLAAAV 169 Query: 129 TLMTRDRCGKPLAAQVLISPMLD-----PSMRLLGDAARLQSDLTAETCAASYRQYLPQT 183 ++M RD + Q+LI P+++ PS+ G+ + L + + QY + Sbjct: 170 SIMARDSGEDFIKHQILIYPVVNFVAPTPSLLEFGEGLWI---LDQKIMSWFSEQYFSRE 226 Query: 184 MQRLHPYAAPLEVSRLAGLPPAFIATAECDVLCPEAEKYASSLIRAGVPTQVSRFAGITH 243 + +P A+ + + L LPPA I TAE D L E E + L RAGV + R+ G+ H Sbjct: 227 EDKFNPLASVI-FADLENLPPALIITAEYDPLRDEGEVFGQMLRRAGVEASIVRYRGVLH 285 Query: 244 AALRTHLPLL 253 + + P+L Sbjct: 286 GFI-NYYPVL 294 >Rv1400c [I] COG0657 Esterase/lipase Mtu Mycobacterium tuberculosis H37Rv Length = 320 Score = 135 bits (340), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 98/260 (37%), Positives = 131/260 (50%), Gaps = 11/260 (4%) Query: 6 SLDIRDFFVPGPLGRIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTPARFIA 65 L + D + GP G I R+Y P T + + P+V+YFHGGGFV G L+ D R A Sbjct: 53 ELRVVDLAIDGPAGPIGTRIYWPPTCPDQAEA-PVVLYFHGGGFVMGDLDTHDGTCRQHA 111 Query: 66 EHGQLAVLSVGYALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLA 125 V+SV Y LAP P+PAA EDA+AA W ++ ++ DL R+ VAGD AGG +A Sbjct: 112 VGADAIVVSVDYRLAPEHPYPAAIEDAWAATRWVAEHGRQVGADLGRIAVAGDSAGGTIA 171 Query: 126 ASLTLMTRDRCGKPLAAQVLISP--MLDPSMRLLGDAARLQSDLTAETCAASYRQYLPQT 183 A + RD G P+ Q+L P + D S+ L + A L + AA R Y + Sbjct: 172 AVIAQRARDMGGPPIVFQLLWYPSTLWDQSLPSLAENADAPI-LDVKAIAAFSRWYAGEI 230 Query: 184 MQRLHPYAAPLEVSR---LAGLPPAFIATAECDVLCPEAEKYASSLIRAGVPTQVSRFAG 240 LH AP+ R LA LPPA+IA A D L + +Y L AGVP +V Sbjct: 231 --DLHNPPAPMAPGRAENLADLPPAYIAVAGYDPLRDDGIRYGELLAAAGVPVEVHNAQT 288 Query: 241 ITHAALRTH--LPLLTEIVN 258 + H + +P TE N Sbjct: 289 LVHGYVGYAGVVPAATEATN 308 >MT1444 [I] COG0657 Esterase/lipase MtC Mycobacterium tuberculosis CDC1551 Length = 320 Score = 134 bits (337), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 98/260 (37%), Positives = 130/260 (50%), Gaps = 11/260 (4%) Query: 6 SLDIRDFFVPGPLGRIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTPARFIA 65 L + D GP G I R+Y P T + + P+V+YFHGGGFV G L+ D R A Sbjct: 53 ELRVVDLAXDGPAGPIGTRIYWPPTCPDQAEA-PVVLYFHGGGFVMGDLDTHDGTCRQHA 111 Query: 66 EHGQLAVLSVGYALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLA 125 V+SV Y LAP P+PAA EDA+AA W ++ ++ DL R+ VAGD AGG +A Sbjct: 112 VGADAIVVSVDYRLAPEHPYPAAIEDAWAATRWVAEHGRQVGADLGRIAVAGDSAGGTIA 171 Query: 126 ASLTLMTRDRCGKPLAAQVLISP--MLDPSMRLLGDAARLQSDLTAETCAASYRQYLPQT 183 A + RD G P+ Q+L P + D S+ L + A L + AA R Y + Sbjct: 172 AVIAQRARDMGGPPIVFQLLWYPSTLWDQSLPSLAENADAPI-LDVKAIAAFSRWYAGEI 230 Query: 184 MQRLHPYAAPLEVSR---LAGLPPAFIATAECDVLCPEAEKYASSLIRAGVPTQVSRFAG 240 LH AP+ R LA LPPA+IA A D L + +Y L AGVP +V Sbjct: 231 --DLHNPPAPMAPGRAENLADLPPAYIAVAGYDPLRDDGIRYGELLAAAGVPVEVHNAQT 288 Query: 241 ITHAALRTH--LPLLTEIVN 258 + H + +P TE N Sbjct: 289 LVHGYVGYAGVVPAATEATN 308 >Rv1399c [I] COG0657 Esterase/lipase Mtu Mycobacterium tuberculosis H37Rv Length = 319 Score = 129 bits (325), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 92/232 (39%), Positives = 121/232 (52%), Gaps = 9/232 (3%) Query: 18 LGRIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTPARFIAEHGQLAVLSVGY 77 L IPVR+Y P P+ R P+V+Y+HGGG+ G L+ D AR A Q V+SV Y Sbjct: 64 LTDIPVRVYWP--PVVR-DNLPVVVYYHGGGWSLGGLDTHDPVARAHAVGAQAIVVSVDY 120 Query: 78 ALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAASLTLMTRDRCG 137 LAP P+PA +D++AA W +NA EL D R+ VAGD AGG ++A + + RD G Sbjct: 121 RLAPEHPYPAGIDDSWAALRWVGENAAELGGDPSRIAVAGDSAGGNISAVMAQLARDVGG 180 Query: 138 KPLAAQVLISP--MLD---PSMRLLGDAARLQSDLTAETCAASYRQYLPQTMQRLHPYAA 192 PL Q+L P M D PS DA L D+ + A Y L + + P Sbjct: 181 PPLVFQLLWYPTTMADLSLPSFTENADAPILDRDVI-DAFLAWYVPGLDISDHTMLPTTL 239 Query: 193 PLEVSRLAGLPPAFIATAECDVLCPEAEKYASSLIRAGVPTQVSRFAGITHA 244 + L+GLPPAFI TAE D L + YA L AGV ++S + H Sbjct: 240 APGNADLSGLPPAFIGTAEHDPLRDDGACYAELLTAAGVSVELSNEPTMVHG 291 >MT1443 [I] COG0657 Esterase/lipase MtC Mycobacterium tuberculosis CDC1551 Length = 319 Score = 129 bits (325), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 92/232 (39%), Positives = 121/232 (52%), Gaps = 9/232 (3%) Query: 18 LGRIPVRLYLPATPLARGQKRPLVMYFHGGGFVSGSLEDADTPARFIAEHGQLAVLSVGY 77 L IPVR+Y P P+ R P+V+Y+HGGG+ G L+ D AR A Q V+SV Y Sbjct: 64 LTDIPVRVYWP--PVVR-DNLPVVVYYHGGGWSLGGLDTHDPVARAHAVGAQAIVVSVDY 120 Query: 78 ALAPAKPFPAAPEDAFAAATWACKNATELKVDLDRMVVAGDDAGGCLAASLTLMTRDRCG 137 LAP P+PA +D++AA W +NA EL D R+ VAGD AGG ++A + + RD G Sbjct: 121 RLAPEHPYPAGIDDSWAALRWVGENAAELGGDPSRIAVAGDSAGGNISAVMAQLARDVGG 180 Query: 138 KPLAAQVLISP--MLD---PSMRLLGDAARLQSDLTAETCAASYRQYLPQTMQRLHPYAA 192 PL Q+L P M D PS DA L D+ + A Y L + + P Sbjct: 181 PPLVFQLLWYPTTMADLSLPSFTENADAPILDRDVI-DAFLAWYVPGLDISDHTMLPTTL 239 Query: 193 PLEVSRLAGLPPAFIATAECDVLCPEAEKYASSLIRAGVPTQVSRFAGITHA 244 + L+GLPPAFI TAE D L + YA L AGV ++S + H Sbjct: 240 APGNADLSGLPPAFIGTAEHDPLRDDGACYAELLTAAGVSVELSNEPTMVHG 291