BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0157.p (250 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ZyhaF [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolas... 134 2e-31 ECs4004 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldol... 134 2e-31 yhaF [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase... 132 6e-31 STM3249 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldol... 130 3e-30 RSc1591 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldol... 127 2e-29 mlr0073 Non COG 127 3e-29 BMEI1120 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldo... 126 4e-29 STM1106 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldol... 124 2e-28 STM2289 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldol... 124 2e-28 AGc1872 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldol... 121 1e-27 >ZyhaF [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase EcZ Escherichia coli O157:H7 EDL933 Length = 256 Score = 134 bits (337), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 79/235 (33%), Positives = 123/235 (52%), Gaps = 5/235 (2%) Query: 4 NRLRTLFAQQQLALSGWVSMGNALLAEVIAHSGYDAVTVDLQHGPFSIDAALPMLQAISS 63 N+ + A +Q+ + W ++ N + EV+ +G+D + +D +H P I +P L A+ Sbjct: 8 NKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWLVLDGEHAPNDISTFIPQLMALKG 67 Query: 64 TPAVPLVRCSENSLGEINKLLDAGAYGVICPLINTAADAERFVRACHYSPRGGRSYGPA- 122 + + P+VR N I +LLD G Y + P + T +AE+ V + Y P G R + Sbjct: 68 SASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVETKEEAEQAVASTRYPPEGIRGVSVSH 127 Query: 123 RGFLYGG-ADYLTHANATLLTLAMIETREGYANAEAILQTPDLDGIFIGPSDLAIELG-L 180 R ++G ADY +N + L IE+++G N +AI T +DGIF+GPSDLA LG L Sbjct: 128 RANMFGTVADYFAQSNKNITILVQIESQQGVDNVDAIAATEGVDGIFVGPSDLAAALGHL 187 Query: 181 APDAYDDVRLNEAIDHLLALARKLGKYVGIFAGTMEMAQRMKGIGMDLVVPGTDI 235 ++ DV+ +AI H+ A GK GI A A+R G V G+D+ Sbjct: 188 GNASHPDVQ--KAIQHIFNRASAHGKPSGILAPVEADARRYLEWGATFVAVGSDL 240 >ECs4004 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase Ecs Escherichia coli O157:H7 Length = 256 Score = 134 bits (337), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 79/235 (33%), Positives = 123/235 (52%), Gaps = 5/235 (2%) Query: 4 NRLRTLFAQQQLALSGWVSMGNALLAEVIAHSGYDAVTVDLQHGPFSIDAALPMLQAISS 63 N+ + A +Q+ + W ++ N + EV+ +G+D + +D +H P I +P L A+ Sbjct: 8 NKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWLVLDGEHAPNDISTFIPQLMALKG 67 Query: 64 TPAVPLVRCSENSLGEINKLLDAGAYGVICPLINTAADAERFVRACHYSPRGGRSYGPA- 122 + + P+VR N I +LLD G Y + P + T +AE+ V + Y P G R + Sbjct: 68 SASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVETKEEAEQAVASTRYPPEGIRGVSVSH 127 Query: 123 RGFLYGG-ADYLTHANATLLTLAMIETREGYANAEAILQTPDLDGIFIGPSDLAIELG-L 180 R ++G ADY +N + L IE+++G N +AI T +DGIF+GPSDLA LG L Sbjct: 128 RANMFGTVADYFAQSNKNITILVQIESQQGVDNVDAIAATEGVDGIFVGPSDLAAALGHL 187 Query: 181 APDAYDDVRLNEAIDHLLALARKLGKYVGIFAGTMEMAQRMKGIGMDLVVPGTDI 235 ++ DV+ +AI H+ A GK GI A A+R G V G+D+ Sbjct: 188 GNASHPDVQ--KAIQHIFNRASAHGKPSGILAPVEADARRYLEWGATFVAVGSDL 240 >yhaF [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase Eco Escherichia coli K12 Length = 256 Score = 132 bits (333), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 79/235 (33%), Positives = 122/235 (51%), Gaps = 5/235 (2%) Query: 4 NRLRTLFAQQQLALSGWVSMGNALLAEVIAHSGYDAVTVDLQHGPFSIDAALPMLQAISS 63 N+ + A +Q+ + W ++ N + EV+ +G+D + +D +H P I +P L A+ Sbjct: 8 NKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWLVLDGEHAPNDISTFIPQLMALKG 67 Query: 64 TPAVPLVRCSENSLGEINKLLDAGAYGVICPLINTAADAERFVRACHYSPRGGRSYGPA- 122 + + P+VR N I +LLD G Y + P + T +AE V + Y P G R + Sbjct: 68 SASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVETKEEAELAVASTRYPPEGIRGVSVSH 127 Query: 123 RGFLYGG-ADYLTHANATLLTLAMIETREGYANAEAILQTPDLDGIFIGPSDLAIELG-L 180 R ++G ADY +N + L IE+++G N +AI T +DGIF+GPSDLA LG L Sbjct: 128 RANMFGTVADYFAQSNKNITILVQIESQQGVDNVDAIAATEGVDGIFVGPSDLAAALGHL 187 Query: 181 APDAYDDVRLNEAIDHLLALARKLGKYVGIFAGTMEMAQRMKGIGMDLVVPGTDI 235 ++ DV+ +AI H+ A GK GI A A+R G V G+D+ Sbjct: 188 GNASHPDVQ--KAIQHIFNRASAHGKPSGILAPVEADARRYLEWGATFVAVGSDL 240 >STM3249 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase Sty Salmonella typhimurium LT2 Length = 256 Score = 130 bits (326), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 76/244 (31%), Positives = 124/244 (50%), Gaps = 5/244 (2%) Query: 4 NRLRTLFAQQQLALSGWVSMGNALLAEVIAHSGYDAVTVDLQHGPFSIDAALPMLQAISS 63 N+ + A QQ+ + W ++ + + EV+ +G+D + +D +H P + +P L A+ Sbjct: 8 NKFKAALAAQQVQIGCWSALASPITTEVLGLAGFDWLVLDGEHAPNDVTTLIPQLMALKG 67 Query: 64 TPAVPLVRCSENSLGEINKLLDAGAYGVICPLINTAADAERFVRACHYSPRGGRSYGPA- 122 + + P+VR N I ++LD G Y + P + T +A R V + Y P G R + Sbjct: 68 SASAPVVRVPTNEPVIIKRMLDIGFYNFLIPFVETQEEAARAVASTRYPPEGIRGVSVSH 127 Query: 123 RGFLYGGA-DYLTHANATLLTLAMIETREGYANAEAILQTPDLDGIFIGPSDLAIELG-L 180 R ++G DY +N + + IE++ G N +AI T +DGIF+GPSDLA LG L Sbjct: 128 RANMFGTVPDYFAQSNKNITIIVQIESQLGVDNVDAIAATEGVDGIFVGPSDLAAALGHL 187 Query: 181 APDAYDDVRLNEAIDHLLALARKLGKYVGIFAGTMEMAQRMKGIGMDLVVPGTDIQQIKA 240 ++ DV+ + I H+ A A+ GK GI A A+R G V G+D+ +A Sbjct: 188 GNASHPDVQ--QTIQHIFARAKAHGKPCGILAPVEADARRYLEWGATFVAVGSDLGAFRA 245 Query: 241 EGAR 244 + Sbjct: 246 STQK 249 >RSc1591 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase Rso Ralstonia solanacearum Length = 272 Score = 127 bits (320), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 76/236 (32%), Positives = 125/236 (52%), Gaps = 6/236 (2%) Query: 4 NRLRTLFAQQQLALSGWVSMGNALLAEVIAHSGYDAVTVDLQHGPFSIDAALPMLQAISS 63 N + A+++ + W++M + AE++A +G+D + +D +H P + + L LQA++ Sbjct: 6 NPFKRALAERRPQIGLWLAMAHPYPAEIVAGAGFDWLLIDGEHAPNQLQSTLAQLQAVAP 65 Query: 64 TPAVPLVRCSENSLGEINKLLDAGAYGVICPLINTAADAERFVRACHYSPRGGRSYGP-- 121 PA P+VR + N + EI +LLD GA ++ P++ +A +A V Y P G R G Sbjct: 66 YPAHPIVRPAWNDVVEIKRLLDIGAQTLLIPMVQSAEEARAAVATMRYPPHGNRGVGSAL 125 Query: 122 ARGFLYGGA-DYLTHANATLLTLAMIETREGYANAEAILQTPDLDGIFIGPSDLAIELG- 179 AR + DY+ A+A + TL +ET G + +AIL +DG+FIGP DLA +G Sbjct: 126 ARASRWNRVDDYVRRADAEMCTLVQVETPRGVEHLDAILAVAGVDGVFIGPGDLAASMGH 185 Query: 180 LAPDAYDDVRLNEAIDHLLALARKLGKYVGIFAGTMEMAQRMKGIGMDLVVPGTDI 235 L + +VR + ID + GK GI + +A+ +G V G+D+ Sbjct: 186 LGEPGHPEVR--QTIDRTIRRIVAAGKAAGILSADPALARHYLSLGATFVAVGSDV 239 >mlr0073 Non COG Length = 257 Score = 127 bits (318), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 75/234 (32%), Positives = 118/234 (50%), Gaps = 5/234 (2%) Query: 6 LRTLFAQQQLALSGWVSMGNALLAEVIAHSGYDAVTVDLQHGPFSIDAALPMLQAISSTP 65 L++ A + ++ W + +AL E++A G+DA+T+D+QHG D+ L L + + Sbjct: 3 LKSRLAADETLVTAWSGVPDALTVEILAKQGFDAITLDMQHGGHHEDSVLRGLMPVLAAN 62 Query: 66 AVPLVRCSENSLGEINKLLDAGAYGVICPLINTAADAERFVRACHYSPRGGRSYGPARGF 125 LVR ++ LD GA VI P++N+ ADA+ F A Y P G RS+GP F Sbjct: 63 KPALVRIPVGRFDMASRALDFGAEAVIAPMVNSVADAKLFAAAMKYPPLGERSWGPTYAF 122 Query: 126 LYGG----ADYLTHANATLLTLAMIETREGYANAEAILQTPDLDGIFIGPSDLAIELGLA 181 G A++L N + AM+ETR + IL TP +DGIF+GPSD +I Sbjct: 123 PRHGKGDHAEWLRDTNQRTMAFAMVETRAALDALDGILDTPGIDGIFLGPSDFSIAWTNG 182 Query: 182 PDAYDDVR-LNEAIDHLLALARKLGKYVGIFAGTMEMAQRMKGIGMDLVVPGTD 234 + + E + + RK GK+ I+ +A R+ +G L+ G++ Sbjct: 183 ATVSSTLESMMETVASVAERTRKAGKHAAIYVVEPAIAGRVVSMGYRLLATGSE 236 >BMEI1120 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase Bme Brucella melitensis Length = 251 Score = 126 bits (317), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 88/243 (36%), Positives = 124/243 (51%), Gaps = 7/243 (2%) Query: 14 QLALSGWVSMGNALLAEVIAHSGYDAVTVDLQHGPFSIDAALPMLQAISSTPAVPLVRCS 73 + LS W S+ L E++AHS +DAVT+D+QHG + L L + + P+VR Sbjct: 9 ETVLSAWSSLPEPLTVEILAHSAFDAVTLDMQHGGHDEASILRSLGLVLNAGKPPVVRIP 68 Query: 74 ENSLGEINKLLDAGAYGVICPLINTAADAERFVRACHYSPRGGRSYGPARGFLYGGA--- 130 ++ LD GA+ VI P+IN+ DA RF A Y P G RS+G R GA Sbjct: 69 VGRFDMASRALDFGAHAVIAPMINSVDDARRFAAAMKYPPVGERSWGVFRANADYGAPGS 128 Query: 131 -DYLTHANATLLTLAMIETREGYANAEAILQTPDLDGIFIGPSDLAIEL--GLAPDAYDD 187 DYLT AN L AMIETR+ Y +AIL +DG+F+GPSD +I G + + D Sbjct: 129 NDYLTTANHDTLAFAMIETRDAYDALDAILDVRGIDGVFVGPSDFSIAWSNGREANPHSD 188 Query: 188 VRLNEAIDHLLALARKLGKYVGIFAGTMEMAQRMKGIGMDLVVPGTDIQQIKAEGARRVG 247 + E I ++ A GK GI++ + E A+R +G + D I+ V Sbjct: 189 A-IVEPISNIAHKAAAAGKIAGIYSPSPEFARRYISLGFRFLTISNDSGYIRLAAETLVK 247 Query: 248 ALR 250 A+R Sbjct: 248 AVR 250 >STM1106 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase Sty Salmonella typhimurium LT2 Length = 263 Score = 124 bits (311), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 75/218 (34%), Positives = 116/218 (53%), Gaps = 4/218 (1%) Query: 20 WVSMGNALLAEVIAHSGYDAVTVDLQHGPFSIDAALPMLQAISSTPAVPLVRCSENSLGE 79 W+ + N+ AE++A +G+D + +D +H P ++ L LQAI+ P+ P+VR S N + Sbjct: 19 WLGLANSYSAELLAGAGFDWLLIDGEHAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQ 78 Query: 80 INKLLDAGAYGVICPLINTAADAERFVRACHYSPRGGRSYGP--ARGFLYGG-ADYLTHA 136 I +LLD GA ++ P++ A +A V A Y P G R G AR + +YL A Sbjct: 79 IKQLLDVGAQTLLIPMVQNADEARNAVAATRYPPAGIRGVGSALARASRWNRIPEYLHLA 138 Query: 137 NATLLTLAMIETREGYANAEAILQTPDLDGIFIGPSDLAIELGLAPDAYDDVRLNEAIDH 196 N + L IETRE +N +IL +DG+FIGP+DL+ ++G A + + AI++ Sbjct: 139 NDAMCVLVQIETREAMSNLASILDVDGIDGVFIGPADLSADMGFAGNPQHP-EVQAAIEN 197 Query: 197 LLALARKLGKYVGIFAGTMEMAQRMKGIGMDLVVPGTD 234 + R GK GI +A+R +G V G D Sbjct: 198 AIVQIRAAGKAPGILMANEALAKRYLELGALFVAVGVD 235 >STM2289 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase Sty Salmonella typhimurium LT2 Length = 267 Score = 124 bits (311), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 75/218 (34%), Positives = 113/218 (51%), Gaps = 4/218 (1%) Query: 20 WVSMGNALLAEVIAHSGYDAVTVDLQHGPFSIDAALPMLQAISSTPAVPLVRCSENSLGE 79 W+S + +AE+ A SGYD + +D +H P ++ LQAI+ + P++R E S Sbjct: 23 WLSSTTSYMAEIAATSGYDWLLIDGEHAPNTVQDLYHQLQAIAPYASQPVIRPIEGSKAL 82 Query: 80 INKLLDAGAYGVICPLINTAADAERFVRACHYSPRGGRSYGP--ARGFLYGGAD-YLTHA 136 I ++LD GA ++ P+++TA A + V A Y P G R G AR +G D Y+ A Sbjct: 83 IKQVLDIGAQTLLIPMVDTAEQARQVVSATRYPPLGQRGVGASVARAARWGRIDNYMAQA 142 Query: 137 NATLLTLAMIETREGYANAEAILQTPDLDGIFIGPSDLAIELGLAPDAYDDVRLNEAIDH 196 N +L L +E++ N +AIL+ +DG+FIGP+DL+ LG PD + I+ Sbjct: 143 NESLCLLVQVESKVALENLDAILEVEGIDGVFIGPADLSASLGY-PDNAGHPEVQRIIEA 201 Query: 197 LLALARKLGKYVGIFAGTMEMAQRMKGIGMDLVVPGTD 234 + R GK G A MAQ+ G + V G D Sbjct: 202 CIYRIRAAGKAAGFLAVDPAMAQKCLAWGANFVAVGVD 239 >AGc1872 [G] COG3836 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 266 Score = 121 bits (304), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 75/239 (31%), Positives = 120/239 (50%), Gaps = 4/239 (1%) Query: 3 ENRLRTLFAQQQLALSGWVSMGNALLAEVIAHSGYDAVTVDLQHGPFSIDAALPMLQAIS 62 ENR +T + + W+ MG A+ AE+ +G+D + +D +HGP + + + L+A++ Sbjct: 5 ENRFKTAIHAGKPQIGLWLDMGEAITAEIAGTAGFDWLVIDGEHGPNDLRSIIDQLRALA 64 Query: 63 STPAVPLVRCSENSLGEINKLLDAGAYGVICPLINTAADAERFVRACHYSPRGGRSYGP- 121 ++PA P+VR I +LLDAGA ++ P++++A A V A HY PRG R G Sbjct: 65 TSPAEPVVRVPVGESWMIKQLLDAGARTLLVPMVDSAEQASDLVSAMHYPPRGIRGMGAA 124 Query: 122 -ARGFLYGG-ADYLTHANATLLTLAMIETREGYANAEAILQTPDLDGIFIGPSDLAIELG 179 AR + ADY A+ + L ETR + + IL +DG+FIGP+DLA ++G Sbjct: 125 VARASAFNTIADYADSASDGVCLLVQAETRAAINDLDNILAVEGVDGVFIGPADLAADMG 184 Query: 180 LAPDAYDDVRLNEAIDHLLALARKLGKYVGIFAGTMEMAQRMKGIGMDLVVPGTDIQQI 238 D+ + I+ + GK GI +R +G V G D+ + Sbjct: 185 YL-GRIDEPEVQAVIEAAIVKIVAAGKAAGILTFNEAYNRRYLELGASFVAVGADVTEF 242