BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0045.p (210 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value XF0378 [H] COG0352 Thiamine monophosphate synthase Xfa Xylella f... 171 1e-42 PA3976 [H] COG0352 Thiamine monophosphate synthase Pae Pseudomon... 137 2e-32 BS_thiC [H] COG0352 Thiamine monophosphate synthase Bsu Bacillus... 128 1e-29 sll0635 [H] COG0352 Thiamine monophosphate synthase Syn Synechoc... 126 4e-29 Ta1277 [H] COG0352 Thiamine monophosphate synthase Tac Thermopla... 121 9e-28 MA2722 [H] COG0352 Thiamine monophosphate synthase Mac Methanosa... 121 1e-27 CAC0495 [H] COG0352 Thiamine monophosphate synthase Cac Clostrid... 120 2e-27 BH1431 [H] COG0352 Thiamine monophosphate synthase Bha Bacillus ... 113 4e-25 SP0725 [H] COG0352 Thiamine monophosphate synthase Spn Streptoco... 110 2e-24 TVN0508 [H] COG0352 Thiamine monophosphate synthase Tvo Thermopl... 109 5e-24 >XF0378 [H] COG0352 Thiamine monophosphate synthase Xfa Xylella fastidiosa 9a5c Length = 234 Score = 171 bits (433), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 86/184 (46%), Positives = 121/184 (65%), Gaps = 2/184 (1%) Query: 10 RGLYIVTPDWDDTAQLLAATELALQQGAALVQYRHKTADAQQRQAQASALLALCRQYQVP 69 RG+Y++TPD DTA+L+A T L G +QYR+K A+ R QA ALLALCRQ +P Sbjct: 35 RGIYLITPDETDTARLIARTA-PLLNGIVWLQYRNKLANTALRTEQAQALLALCRQTGIP 93 Query: 70 LIINDHVDLCLDIDADGIHVGGTDASIAEARKAVGPDRIVGASCYGTLELAHAAYRDGAS 129 L+IND ++L I ADG+H+G D++ + AR +GP I+G SCY +E A A + GAS Sbjct: 94 LLINDDLELAQTIGADGVHLGMHDSNPSIARAQLGPHAIIGVSCYNQIERAKQAIKAGAS 153 Query: 130 YVAFGGFYPSRVKKYDFRTAPEIIAHSKREIPLPVVVIGGITLENAPPLVEQGADMVAVI 189 YV FG FYPS K +R PE++ + + +P V IGG+T +N P++E GA+++AVI Sbjct: 154 YVGFGTFYPSHTKTTPYRATPELLRQTT-HLGVPRVAIGGLTPKNIAPIIEAGAELLAVI 212 Query: 190 SSVY 193 S +Y Sbjct: 213 SGIY 216 >PA3976 [H] COG0352 Thiamine monophosphate synthase Pae Pseudomonas aeruginosa Length = 209 Score = 137 bits (345), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 80/205 (39%), Positives = 122/205 (59%), Gaps = 5/205 (2%) Query: 9 LRGLYIVT-PDWDDTAQLLAATELALQQGAALVQYRHKTADAQQRQAQASALLALCRQYQ 67 LRGLY +T D +LL E AL+ GA L+QYR K++D +R +A +L LC ++ Sbjct: 3 LRGLYAITDSQLLDDGRLLPYVEAALRGGARLLQYRDKSSDQARRLREAESLRELCERHG 62 Query: 68 VPLIINDHVDLCLDIDADGIHVGGTDASIAEARKAVGPDRIVGASCYGTLELAHAAYRDG 127 LI+ND +L + G+H+G TD S++ AR +G I+GA+C+ LELA A +G Sbjct: 63 AQLIVNDDAELAARLGV-GLHLGQTDGSLSAARALLGRQAIIGATCHAQLELAEQAVAEG 121 Query: 128 ASYVAFGGFYPSRVKKYDFRTAPEIIAHSKREIPLPVVVIGGITLENAPPLVEQGADMVA 187 ASYVAFG F+ S K + E++ ++ + LP+ IGGI+L+ AP L+ +G D+VA Sbjct: 122 ASYVAFGRFFNSSTKPGAPAASVELLDQARPRLHLPITAIGGISLDTAPGLIARGVDLVA 181 Query: 188 VISSVYLVPVE---ERKTRELADLY 209 VI +++ ER+ R + L+ Sbjct: 182 VIHALFAAASAAEVERRARAFSALF 206 >BS_thiC [H] COG0352 Thiamine monophosphate synthase Bsu Bacillus subtilis Length = 222 Score = 128 bits (321), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 83/216 (38%), Positives = 119/216 (55%), Gaps = 12/216 (5%) Query: 4 EYAKHLRGLYIVTPDWDDTAQLLAATELALQQGAALVQYRHKTADAQQRQAQ---ASALL 60 E K L +Y + + A + + AL+ GA L Q+R K DA +A+ A Sbjct: 7 EMMKELLSVYFIMGSNNTKADPVTVVQKALKGGATLYQFREKGGDALTGEARIKFAEKAQ 66 Query: 61 ALCRQYQVPLIINDHVDLCLDIDADGIHVGGTDASIAEARKAVGPDRIVGASCYGTLELA 120 A CR+ VP I+ND V+L L++ ADGIH+G DA+ E R A+G D I+G S + T+ Sbjct: 67 AACREAGVPFIVNDDVELALNLKADGIHIGQEDANAKEVRAAIG-DMILGVSAH-TMSEV 124 Query: 121 HAAYRDGASYVAFGGFYPSRVKKYDFRTAP--EIIAHSKRE-IPLPVVVIGGITLENAPP 177 A DGA YV G YP+ KK D R +I +R+ I +P+V IGGIT++NA P Sbjct: 125 KQAEEDGADYVGLGPIYPTETKK-DTRAVQGVSLIEAVRRQGISIPIVGIGGITIDNAAP 183 Query: 178 LVEQGADMVAVISSVYLVPVEE---RKTRELADLYR 210 +++ GAD V++IS++ E RK RE Y+ Sbjct: 184 VIQAGADGVSMISAISQAEDPESAARKFREEIQTYK 219 >sll0635 [H] COG0352 Thiamine monophosphate synthase Syn Synechocystis Length = 343 Score = 126 bits (316), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 74/181 (40%), Positives = 103/181 (56%), Gaps = 4/181 (2%) Query: 12 LYIVTPDWDDTAQLLAATELALQQGAALVQYRHKTADAQQRQAQASALLALCRQYQVPLI 71 LY+VT ++ LLA E ALQ G LVQYR K A+ Q R +A L LCRQY+ + Sbjct: 145 LYLVTSPQEN---LLATVEAALQGGLKLVQYRDKDAEDQLRWQRAKDLRELCRQYEALFL 201 Query: 72 INDHVDLCLDIDADGIHVGGTDASIAEARKAVGPDRIVGASCYGTLELAHAAYRDGASYV 131 +ND VDL L +DADG+H+G D IA AR+ +GPD+I+G S E+A A +GA Y+ Sbjct: 202 VNDRVDLALAVDADGVHLGQQDLPIAVARQLLGPDKIIGRSTTNPEEMAK-AIAEGADYI 260 Query: 132 AFGGFYPSRVKKYDFRTAPEIIAHSKREIPLPVVVIGGITLENAPPLVEQGADMVAVISS 191 G Y + K E + ++ P+P IGGI EN ++E GA VA++ + Sbjct: 261 GVGPVYATPTKAGKKPAGLEYVQYAVTNSPVPWFAIGGIDGENLGEVMEAGATQVAIVRA 320 Query: 192 V 192 + Sbjct: 321 I 321 >Ta1277 [H] COG0352 Thiamine monophosphate synthase Tac Thermoplasma acidophilum Length = 213 Score = 121 bits (304), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 75/209 (35%), Positives = 110/209 (52%), Gaps = 12/209 (5%) Query: 1 MKPEYAKHLRGLYIVTPDWDDTAQLLAATELALQQGAALVQYRHKTADAQQRQAQASALL 60 MK E + ++GLY+VT D+ E A+ G +VQYR K+ R A + Sbjct: 1 MKLE-GRRIKGLYLVTEDYS-RKNFFNIIEEAIIGGVDIVQYRDKSNPRSVRLDVARKVK 58 Query: 61 ALCRQYQVPLIINDHVDLCLDIDADGIHVGGTDASIAEARKAVGPDRIVGASCYGTLELA 120 +C +Y + I+D V L +++ ADG+H+G D + +AR+ D ++G S YG E+A Sbjct: 59 QICNRYDILFFIDDDVQLAIEVQADGVHIGKDDMPLPDARRIF--DGLIGYSTYGDREMA 116 Query: 121 HAAYRDGASYVAFGGFYPSRVKK----YDFRTAPEIIAHSKREIPLPVVVIGGITLENAP 176 A ++GA YVAFG F+ + KK YD I + I +PV VIGGI + N Sbjct: 117 IFAEKNGADYVAFGPFFHTDTKKDADVYDIHVLEGI----HKYIRIPVFVIGGINISNIR 172 Query: 177 PLVEQGADMVAVISSVYLVPVEERKTREL 205 G D VAV+S+++ P ER REL Sbjct: 173 TFSGYGIDGVAVVSAIFSDPDPERAAREL 201 >MA2722 [H] COG0352 Thiamine monophosphate synthase Mac Methanosarcina acetivorans str.C2A Length = 228 Score = 121 bits (303), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 69/200 (34%), Positives = 106/200 (53%), Gaps = 3/200 (1%) Query: 12 LYIVTPDWDDTAQLLAATELALQQGAALVQYRHKTADAQQRQAQASALLALCRQYQVPLI 71 Y+VT L+ A+ G +VQYR K ++ +AS + +C + + Sbjct: 23 FYLVTDSGLSKKGTLSDVREAVDAGCKIVQYREKNKSTKEMIDEASEIKRICGDRAI-FL 81 Query: 72 INDHVDLCLDIDADGIHVGGTDASIAEARKAVGPDRIVGASCYGTLELAHAAYRDGASYV 131 +ND +D+ L +DADG+H+G D I A+K +GP++I+G + + +E A A R+GA YV Sbjct: 82 VNDRIDVALAVDADGVHIGQDDMPIEIAKKLLGPEKIIGLTVH-NVEEALEAERNGADYV 140 Query: 132 AFGGFYPSRVKKYDFR-TAPEIIAHSKREIPLPVVVIGGITLENAPPLVEQGADMVAVIS 190 G + + KK + P I K I +PVV IGGI EN P+VE GAD IS Sbjct: 141 GLGSIFDTSTKKDAGKGIGPASIKEVKNAIKIPVVAIGGINRENCIPVVENGADSFVAIS 200 Query: 191 SVYLVPVEERKTRELADLYR 210 +V +R+TR+ ++ R Sbjct: 201 AVVCSDDVKRETRKFIEIIR 220 >CAC0495 [H] COG0352 Thiamine monophosphate synthase Cac Clostridium acetobutylicum Length = 211 Score = 120 bits (301), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 65/186 (34%), Positives = 104/186 (55%), Gaps = 1/186 (0%) Query: 25 LLAATELALQQGAALVQYRHKTADAQQRQAQASALLALCRQYQVPLIINDHVDLCLDIDA 84 L + E A++ G LVQ R K A L + Y++PLIIND +D+ L I+A Sbjct: 22 LYKSIEEAIKGGVTLVQLREKEMSTLDFYESALKLKKITETYKIPLIINDRIDIALAINA 81 Query: 85 DGIHVGGTDASIAEARKAVGPDRIVGASCYGTLELAHAAYRDGASYVAFGGFYPSRVKKY 144 DG+H+G +D + +AR+ +G D+I+G S + ++E A A R+GA+Y+ G Y + K Sbjct: 82 DGVHIGQSDMPLIKARELLGKDKIIGVSAH-SIEEALEAERNGATYLGVGAIYNTSTKGD 140 Query: 145 DFRTAPEIIAHSKREIPLPVVVIGGITLENAPPLVEQGADMVAVISSVYLVPVEERKTRE 204 + E + + K + +PVV IGGI ENA ++E G D ++VIS + + K R Sbjct: 141 AQAVSLEELKNIKNSVKIPVVGIGGINEENANKVIETGVDGISVISGILSAQKIKDKARV 200 Query: 205 LADLYR 210 + D+ + Sbjct: 201 MFDIVK 206 >BH1431 [H] COG0352 Thiamine monophosphate synthase Bha Bacillus halodurans Length = 211 Score = 113 bits (282), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 69/186 (37%), Positives = 107/186 (57%), Gaps = 9/186 (4%) Query: 12 LYIVT-PDWDDTAQLLAATELALQQGAALVQYRHKTADAQQRQAQASALLALCRQYQVPL 70 LY +T ++ L A E A+ G ++Q R K + ++ +A L AL ++Y +PL Sbjct: 6 LYAITGEEFHPDRSLQAVMEEAILGGVDIIQLRDKKSHKREVLEKARVLQALAKKYDIPL 65 Query: 71 IINDHVDLCLDIDADGIHVGGTDASIAEARKAVGPDRIVGASCYGTLELAHAAYRDGASY 130 IINDH+D+ L +DADGIH+G D ++EARK +G D+I+G S + +E A A + GA Y Sbjct: 66 IINDHIDVALAVDADGIHLGQDDLPLSEARKIMGRDKIIGISTH-KIEEAREAEKGGADY 124 Query: 131 VAFGGFYPSRVKK--YDFRTAPEI--IAHSKREIPLPVVVIGGITLENAPPLVEQGADMV 186 + G + ++ K+ D T I +AH EI +P V IGGI L N +++ GA + Sbjct: 125 IGVGPIFETKSKEDVVDPVTTAYIQQVAH---EISIPFVAIGGIKLHNVEQVLDAGATRI 181 Query: 187 AVISSV 192 +IS + Sbjct: 182 CMISEI 187 >SP0725 [H] COG0352 Thiamine monophosphate synthase Spn Streptococcus pneumoniae TIGR4 Length = 210 Score = 110 bits (275), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 66/193 (34%), Positives = 104/193 (53%), Gaps = 2/193 (1%) Query: 12 LYIVTPDWDDTAQ-LLAATELALQQGAALVQYRHKTADAQQRQAQASALLALCRQYQVPL 70 LY+VT + D+ + LA E A + G +VQ R K Q A + + YQVPL Sbjct: 8 LYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNLTTNQYYQLAKQVKEITDAYQVPL 67 Query: 71 IINDHVDLCLDIDADGIHVGGTDASIAEARKAVGPDRIVGASCYGTLELAHAAYRDGASY 130 II+D +D+CL +DA G+H+G + ++ ARK +GP++I+G + T++ A A + GA Y Sbjct: 68 IIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGPEKILGVTAK-TVKRALEAEKSGADY 126 Query: 131 VAFGGFYPSRVKKYDFRTAPEIIAHSKREIPLPVVVIGGITLENAPPLVEQGADMVAVIS 190 + G +P+ K+ T + + + +PVV IGG+T EN L+ G VAV+ Sbjct: 127 LGTGAIFPTTTKENAPITLISTLKTICQTVAIPVVAIGGLTSENIDQLMGTGIAGVAVVR 186 Query: 191 SVYLVPVEERKTR 203 + E KT+ Sbjct: 187 DLMQAEDIEAKTQ 199 >TVN0508 [H] COG0352 Thiamine monophosphate synthase Tvo Thermoplasma volcanium Length = 211 Score = 109 bits (272), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 66/190 (34%), Positives = 100/190 (52%), Gaps = 11/190 (5%) Query: 7 KHLRGLYIVTPDWDDTAQLLAATELALQQGAALVQYRHKTADAQQRQAQASALLALCRQY 66 K LRGLY+VT D+ A+ A++ G ++QYR KT + A L ++ Y Sbjct: 4 KKLRGLYLVTYDYY-GAEFYEKIIDAIEGGVDILQYRDKTNAYSVKLQVARKLTSIAADY 62 Query: 67 QVPLIINDHVDLCLDIDADGIHVGGTDASIAEARKAVGPDRIVGASCYGTLELAHAAYRD 126 +P ++D L + ADG+H+G D I + R + ++G S YG LELA A + Sbjct: 63 GIPFFVDDDPKLAKEAGADGVHIGRDDPPIEQVRDVF--NGLIGVSTYGNLELATEAEKK 120 Query: 127 GASYVAFGGFYPSRVKK----YDFRTAPEIIAHSKREIPLPVVVIGGITLENAPPLVEQG 182 GASYVAFG F+ + KK YD + I+ ++ + +P+ VIGGI LE Sbjct: 121 GASYVAFGSFFHTDTKKDAGIYDIK----ILEKARHFVKIPIFVIGGINLETITKFCGYS 176 Query: 183 ADMVAVISSV 192 D +AV+S++ Sbjct: 177 IDGIAVVSAI 186