BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0016.p (328 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0147 [R] COG3491 Isopenicillin N synthase and related dioxygen... 247 3e-65 PA4191 [R] COG3491 Isopenicillin N synthase and related dioxygen... 174 2e-43 CC0200 Non COG 152 1e-36 mlr6892 [R] COG3491 Isopenicillin N synthase and related dioxyge... 144 3e-34 YPO1316 [R] COG3491 Isopenicillin N synthase and related dioxyge... 126 9e-29 Cj1199 [R] COG3491 Isopenicillin N synthase and related dioxygen... 113 6e-25 SPAC25B8.13c [R] COG3491 Isopenicillin N synthase and related di... 111 2e-24 mll0111 [R] COG3491 Isopenicillin N synthase and related dioxyge... 100 7e-21 SPCC965.15 [R] COG3491 Isopenicillin N synthase and related diox... 93 8e-19 RSp1529 [R] COG3491 Isopenicillin N synthase and related dioxyge... 65 1e-10 >PA0147 [R] COG3491 Isopenicillin N synthase and related dioxygenases Pae Pseudomonas aeruginosa Length = 320 Score = 247 bits (630), Expect = 3e-65, Method: Compositional matrix adjust. Identities = 127/311 (40%), Positives = 178/311 (57%), Gaps = 1/311 (0%) Query: 12 RIPVIDFADAFSPELARREALAWEIHKISRDVGFFYLVNHGVPQALIDGQFDWARRFFAL 71 R+P+ID A + + +A I + R+ GFFY+ H +P A + AR FFAL Sbjct: 3 RLPIIDIAPLYGADRGAWNDVARRIDQACREWGFFYIAGHPLPAARFEALLAAAREFFAL 62 Query: 72 PQASKSAIDMRHSASGYGYERMGAQALDEGSPADLKEGFQFGFDIAPDHPYVQRGLLRYG 131 P K ID+ S + GY + + LD P+D KE F + +HP V+ G YG Sbjct: 63 PVEEKLKIDITRSRNHRGYGAIATEQLDPALPSDFKETFDMALHLPAEHPDVRAGKSFYG 122 Query: 132 HTLWPQDLPGFETHSRQYYEAVRALSHRLLGVIALSLEMPEDFFTPVLHTPIATQRMLHY 191 P DLPG+E +Y + AL+ +L +A++L + EDFF P++ R++HY Sbjct: 123 PNRHP-DLPGWEALLEGHYADMLALARTVLRALAIALGIEEDFFDRRFEQPVSVFRLIHY 181 Query: 192 PPQPASAQHNQIGAGAHTDWGLVTILAQDAIGGLEICNAEGDWVSAPPIAGSFVVNIGDL 251 PP A +Q GAGAHTD+G VT+L QDA GGL++ N +G+W+ APPI G+FVVNIGD+ Sbjct: 182 PPASARQSADQPGAGAHTDYGCVTLLYQDAAGGLQVQNRQGEWIDAPPIDGTFVVNIGDM 241 Query: 252 LQRWSNDLYHSNAHRVCNVGNAPRYSLPFFQDGDQAAVVACLPTCCSAERPARYAPCTIG 311 + RWSND Y S HRV + RYS+PFF + + CLP C A+ P +Y P T G Sbjct: 242 MARWSNDRYRSTPHRVISPRGVHRYSMPFFAEPHMDTEIRCLPGCFDADNPPKYPPTTCG 301 Query: 312 DYLEMKVKQTF 322 D+L + QT+ Sbjct: 302 DWLTSRFAQTY 312 >PA4191 [R] COG3491 Isopenicillin N synthase and related dioxygenases Pae Pseudomonas aeruginosa Length = 334 Score = 174 bits (441), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 119/330 (36%), Positives = 171/330 (51%), Gaps = 19/330 (5%) Query: 8 MTARRIPVIDFADAFSPELARREALAWEIHKISRDVGFFYLVNHGVPQALIDGQFDWARR 67 M+ +P+ID A + A R ++A I + R+ GFFY+V HGV L++ AR+ Sbjct: 1 MSTPSLPIIDIAALAGSDPAARRSVAVRIDRACREQGFFYVVGHGVEAQLVERLERLARQ 60 Query: 68 FFALPQASKSAIDMRHSASGY-GYERMGAQALDEGSPADLKEGFQFGFDIAPDHPYVQRG 126 FFAL + SK M + GY +G + L P D KEG G ++ +HP V+ G Sbjct: 61 FFALDETSKLRWRMELGGRAWRGYFPLGGE-LTSNRP-DWKEGLYLGSELDAEHPEVRAG 118 Query: 127 LLRYGHTLWPQDLPGFETHSRQYYEAVRALSHRLLGVIALSLEMPEDFFTP-VLHTPIAT 185 +G L+P+ +PG +Y +A + HRL+ IAL L + D+F P+ Sbjct: 119 TPLHGANLFPE-VPGLRETLLEYLDATTRVGHRLMEGIALGLGLEADYFAARYTGDPLIL 177 Query: 186 QRMLHYPPQPASAQHN-QIGAGAHTDWGLVTILAQDAIGGLEICNAEGDWVSAPPIAGSF 244 R+ +YP QP + Q G G HTD+GL+T+L QDAIGGL++ +G W+ APPI GSF Sbjct: 178 FRLFNYPSQPVPEGLDVQWGVGEHTDYGLLTLLHQDAIGGLQVRTPQG-WLEAPPIPGSF 236 Query: 245 VVNIGDLLQRWSNDLYHSNAHRVC-NVGNAPRYSLPFFQDGD---QAAVVACLPTCCSAE 300 V N+GD+L+R + LY S HRV N R S P F D + + + LP + Sbjct: 237 VCNLGDMLERMTGGLYRSTPHRVARNTSGRDRLSFPLFFDPNFHARVQPIEGLPEVPEQD 296 Query: 301 RPA-RYAPCTI-------GDYLEMKVKQTF 322 A R+ + GDYL KV + F Sbjct: 297 DSARRWDQANVHAFHGEYGDYLLNKVAKVF 326 >CC0200 Non COG Length = 311 Score = 152 bits (384), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 92/282 (32%), Positives = 135/282 (47%), Gaps = 12/282 (4%) Query: 44 GFFYLVNHGVPQALIDGQFDWARRFFALPQASKSAIDMRHSASGYGYERMGAQALDEGSP 103 GF L ++ + QA ID D A+ FFALP +K GY G + Sbjct: 33 GFAVLSDYDLDQARIDAAVDSAKAFFALPVETKKQY-AGVKGGARGYIPFGVETAKGADH 91 Query: 104 ADLKEGFQFGFDIAPDHPYVQRGLLRYGHTLWPQDLPGFETHSRQYYEAVRALSHRLLGV 163 DLKE + G D+ P H R +WP ++P F+ Y ++ + ++L Sbjct: 92 YDLKEFWHMGRDLPPGH----RFRAHMADNVWPAEIPAFKHDVSWLYNSLDGMGGKVLEA 147 Query: 164 IALSLEMPEDFFTPVLHTPIATQRMLHYPPQPASAQHNQIGAGAHTDWGLVTILAQDAIG 223 IA L++ DFF P + + R+LHYPP P A + AGAH D +T+L G Sbjct: 148 IATYLKLERDFFKPTVQDGNSVLRLLHYPPIPKDA--TGVRAGAHGDINTITLLLGAEEG 205 Query: 224 GLEICNAEGDWVSAPPIAGSFVVNIGDLLQRWSNDLYHSNAHRVCN----VGNAPRYSLP 279 GLE+ + +G W+ P G V+NIGD+L+R +N++ S HRV N PRYS P Sbjct: 206 GLEVLDRDGQWLPINPPPGCLVINIGDMLERLTNNVLPSTVHRVVNPPPERRGVPRYSTP 265 Query: 280 FFQDGDQAAVVACLPTCCSAERPARYAPCTIGD-YLEMKVKQ 320 FF + L C +AE P RY D +L+ ++++ Sbjct: 266 FFLHFASDYEIKTLQNCVTAENPDRYPESITADEFLQQRLRE 307 >mlr6892 [R] COG3491 Isopenicillin N synthase and related dioxygenases Mlo Mesorhizobium loti Length = 366 Score = 144 bits (363), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 98/279 (35%), Positives = 141/279 (50%), Gaps = 18/279 (6%) Query: 11 RRIPVIDFADAFSPELARREALAWEIHKISRDVGFFYLVNHGVPQALIDGQFDWARRFFA 70 R +PV+D + RR LA ++ SRD+GFFYL HG+ A I +R+FFA Sbjct: 30 RIVPVLDLSRLEQGASERRTFLA-DLRSASRDIGFFYLAGHGISWAEISEVLTASRQFFA 88 Query: 71 LPQASKSAIDMRHSASGYGYERMGAQALDEGSPADLKEGFQFGFDIAPDHPYVQRG---- 126 LP+A K AI+M S+ GY R G + L +G +E ++ DI + + +G Sbjct: 89 LPEADKLAIEMVKSSQFRGYTRAGGE-LTKG-----REDWREQLDIGVERQAIAQGPGTP 142 Query: 127 --LLRYGHTLWPQDLPGFETHSRQYYEAVRALSHRLLGVIALSLEMPEDFFTPVLHT-PI 183 G WP LP + + V A++ RLL A SL+ PED F P+ + P Sbjct: 143 AWTRLQGPNQWPAALPDLKPALLAWQSKVTAVAIRLLKAFAQSLDQPEDAFDPIYSSEPN 202 Query: 184 ATQRMLHYPPQPASAQHNQIGAGAHTDWGLVTILAQDAIGGLEICNAEGDWVSAPPIAGS 243 +++ YP + + G GAH D G +T+L QD GL++ + +G WV PI G+ Sbjct: 203 HRMKIVRYPGRDTTGGDQ--GVGAHKDGGFLTLLLQDDNKGLQV-DYDGSWVDVDPIPGT 259 Query: 244 FVVNIGDLLQRWSNDLYHSNAHRVCN-VGNAPRYSLPFF 281 VVNIG+LL+ SN + HRV R S+PFF Sbjct: 260 LVVNIGELLELASNGYLRATVHRVVTPPAGVERISVPFF 298 >YPO1316 [R] COG3491 Isopenicillin N synthase and related dioxygenases Ype Yersinia pestis Length = 355 Score = 126 bits (316), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 97/283 (34%), Positives = 132/283 (46%), Gaps = 14/283 (4%) Query: 29 REALAWEIHKISRDVGFFYLVNHGVPQALIDGQFDWARRFFALPQASKSAIDMRHSASGY 88 R+A + +RDVGFFYL HG+ +L+ +R+FFAL K +I M S Sbjct: 40 RQAFLTALRHAARDVGFFYLTGHGIDTSLLRQIQILSRQFFALSDEEKLSIAMIRSPHFR 99 Query: 89 GYERMGAQALDEGSPADLKEGFQFGFDIAP-----DHPYVQRGLLRYGHTLWPQDLPGFE 143 GY R A G P D +E F G + P P R G WP LP + Sbjct: 100 GYNR-AASEFTRGQP-DWREQFDIGAERTPLPQIAGAPSWTR---LQGPNQWPTALPELK 154 Query: 144 THSRQYYEAVRALSHRLLGVIALSLEMPEDFFTPVL-HTPIATQRMLHYPPQPASAQHNQ 202 Q+ E + +S RLL AL+L++ E F + P +++ YP + A+ Q Q Sbjct: 155 PVLLQWQEEMTRMSLRLLRAFALALDLDEQAFDALYGDKPNEHIKLIRYPGREAT-QSGQ 213 Query: 203 IGAGAHTDWGLVTILAQDAIGGLEICNAEGDWVSAPPIAGSFVVNIGDLLQRWSNDLYHS 262 G GAH D G ++ L QD GL++ EG W+ A P G+FVVNIG+LL+ SN + Sbjct: 214 -GVGAHKDSGFLSFLLQDTQRGLQVEVEEGQWIDAVPREGTFVVNIGELLELASNGYLRA 272 Query: 263 NAHRVCN-VGNAPRYSLPFFQDGDQAAVVACLPTCCSAERPAR 304 HRV R S+ FF AVV P + AR Sbjct: 273 TVHRVETPPAGIDRLSIAFFLGARLDAVVPLYPLSAALAAEAR 315 >Cj1199 [R] COG3491 Isopenicillin N synthase and related dioxygenases Cje Campylobacter jejuni Length = 330 Score = 113 bits (283), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 75/276 (27%), Positives = 130/276 (47%), Gaps = 20/276 (7%) Query: 12 RIPVIDFADAFSPELARREALAWEIHKISRDVGFFYLVNHGVPQALIDGQFDWARRFFAL 71 +P++D A+ + + + +I+ +GFFYL+N + + L + F + FF L Sbjct: 2 NLPILDL-QAYEKD---KSTFLKNLREIASKIGFFYLINTSIDKNLNEKLFKLGKEFFNL 57 Query: 72 PQASKSAIDMRHSASGYGYERMGAQALDEGSPADLKEGFQFG-----FDIAPDHPYVQRG 126 ++SK I M HS GY G + D +E G + + P QR Sbjct: 58 SRSSKELISMVHSPQFRGYTSEGFEY--TAGSKDYREQLDIGTERDALNWNLNSPLWQR- 114 Query: 127 LLRYGHTLWPQDLPGFETHSRQYYEAVRALSHRLLGVIALSLEMPEDFFTPVL-HTPIAT 185 G LWP ++P + +++ + +LL A +L++P + F + Sbjct: 115 --LEGPNLWPSEIPELKKTFLTWHKQTKKACLKLLKAFAQALDLPNNAFDKLYGENSYEH 172 Query: 186 QRMLHYPPQPASAQHNQIGAGAHTDWGLVTILAQDAIGGLEICNAEGDWVSAPPIAGSFV 245 +++HY P S+++ G G+H D GL+T + Q+ G E +G W+S PP+ S V Sbjct: 173 CKIIHY---PKSSKNITQGVGSHKDGGLITFVFQEKQSGFEAF-IDGKWLSIPPLENSVV 228 Query: 246 VNIGDLLQRWSNDLYHSNAHRVCNVGNAPRYSLPFF 281 VNIG+ L+ +N + HRV N+ R+S+ +F Sbjct: 229 VNIGEFLELATNGYLKATIHRV-NLSPKERFSIAYF 263 >SPAC25B8.13c [R] COG3491 Isopenicillin N synthase and related dioxygenases Spo Schizosaccharomyces pombe Length = 397 Score = 111 bits (278), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 70/231 (30%), Positives = 116/231 (50%), Gaps = 20/231 (8%) Query: 99 DEGSPADLKEGFQFGFD-IAPDHPYVQRGLLR--YGHTLWPQDL-PGFETHSRQYYEAVR 154 DE + +E F FG D ++ D LLR G WP F +Y++ + Sbjct: 174 DESISNEFRESFYFGNDNLSKDR------LLRPFQGPNKWPSTAGSSFRKALVKYHDQML 227 Query: 155 ALSHRLLGVIALSLEMPEDFFTPVLHTPIATQRMLHYPPQPASAQHNQIGAGAHTDWGLV 214 A ++ ++ ++A SLE+ D F P + R+L YP P N++G HTD + Sbjct: 228 AFANHVMSLLAESLELSPDAFDEFCSDPTTSIRLLRYPSSP-----NRLGVQEHTDADAL 282 Query: 215 TILAQDAIGGLEICNAEGD-WVSAPPIAGSFVVNIGDLLQRWSNDLYHSNAHRVCNVGNA 273 T+++QD + GLEI + + ++S P G+ + N+GD++ +N+ Y S+ HRVCN + Sbjct: 283 TLMSQDNVKGLEILDPVSNCFLSVSPAPGALIANLGDIMAILTNNRYKSSMHRVCNNSGS 342 Query: 274 PRYSLPFFQDGDQAAVVACLPTCCSAERPARYAPCTIGDYLEMKVKQTFGA 324 RY++PFF G+ VVA LP P+ P + D L + ++ + Sbjct: 343 DRYTIPFFLQGNIDYVVAPLPGLG----PSTAEPIAVEDLLRDHFQNSYTS 389 >mll0111 [R] COG3491 Isopenicillin N synthase and related dioxygenases Mlo Mesorhizobium loti Length = 342 Score = 99.8 bits (247), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 95/331 (28%), Positives = 136/331 (41%), Gaps = 47/331 (14%) Query: 13 IPVIDFADAFSPELARREALAWEIHKISRDVGFFYLVNHGVPQALIDGQFDWARRFFALP 72 I I AD F P R+ I + +GF + + L+ + FALP Sbjct: 5 IETIGIADLFGPPSPGRDRADISIMAAASGIGFMAVRDFPGDAWLMPERRAQLLHIFALP 64 Query: 73 QASKSAI-----DMRHSASGYGYERMGAQALDEGSPADLKEGFQFGFDIA-------PDH 120 K + D G+ + A+ KEG G DIA D Sbjct: 65 DTEKQKLLRWNFDRTRKNVYRGWFSLQPTAVS------YKEGIDMGPDIADAAGVSASDD 118 Query: 121 PYVQRGLLRYGHTLWPQD--LPGFETHSRQYYEAVRALSHRLLGVIALSLEMPEDFFTPV 178 P + T P + LPG+ + YY A+ +S+ L+ IA L++PE F Sbjct: 119 PLCE-------PTPLPTEDALPGWRAAAADYYRAMETVSNALMRSIARGLDLPETIFDSY 171 Query: 179 LHTPIATQRMLHYPPQPASA---------------QHNQIGAGAHTDWGLVTILAQDAIG 223 I+T R++ YP + A+A + I H D G VT+LAQD + Sbjct: 172 FEHGISTLRLIRYPLRDANAGIDISGPEFSVVHKGETRTIIGREHADSGFVTLLAQDGVE 231 Query: 224 GLEICNAEGDWVSAPPIAGSFVVNIGDLLQRWSNDLYHSNAHRVCNVGNAPRYSLPFFQD 283 GL+ N G+W+ PP + VN G LL+RW+ + HRV A R+S+PFF + Sbjct: 232 GLQAKNLAGEWIDVPPANSTLAVNFGQLLERWTGGRVRATRHRVIAPKTA-RFSIPFFYE 290 Query: 284 GDQAAVVACLPTCCSAERPARYAPCTIGDYL 314 A +A LP S P + P GDYL Sbjct: 291 PRVDAEIAPLP--LSGAEP--FEPFLYGDYL 317 >SPCC965.15 [R] COG3491 Isopenicillin N synthase and related dioxygenases Spo Schizosaccharomyces pombe Length = 321 Score = 93.2 bits (230), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 88/331 (26%), Positives = 142/331 (42%), Gaps = 37/331 (11%) Query: 8 MTARRIPVIDFADAFSPELARREALAWEIHKISRDVGFFYLVNHGVPQALIDGQFDWARR 67 M + +P ID ++ + + + E+ ++ GF L NHG+P ID F A + Sbjct: 1 MGSLEVPCIDLSENDTSIVVK------ELLDACKNWGFVSLKNHGIPLDEIDRTFKLADK 54 Query: 68 FFALPQASKSAIDMRHSASGYGYERMGAQALD--EGSPADLKEGFQF-GFDIAPDHPYVQ 124 FF +P K + GY + LD S DLKE + GF PD P ++ Sbjct: 55 FFDIPVEEKQKYLFKGGRLHSGYTGHFGEKLDMEHQSRGDLKESYDLAGF---PD-PKLE 110 Query: 125 RGLLRYGHTLWP---QDLPGFETHSRQYYEAVRALSHRLLGVIALSLEMPEDFFTPVLHT 181 L P + + F R Y+ L+ RLL A+ +P DFF+ + Sbjct: 111 --------NLCPFIAEHMDEFLQFQRHCYK----LTLRLLDFFAIGFGIPPDFFSKSHSS 158 Query: 182 PIATQRMLHYP-PQPASAQHNQIGAGAHTDWGLVTILAQDAIGGLEI----CNAEGDWVS 236 R+L Y P+ + + AGAH+D+G +T+L Q GLEI + DW+ Sbjct: 159 EEDVLRLLKYSIPEGVERREDDEDAGAHSDYGSITLLFQRDAAGLEIRPPNFVKDMDWIK 218 Query: 237 APPIAGSFVVNIGDLLQRWSNDLYHSNAHRV-CNVGNAPRYSLPFFQDGDQAAVVACLPT 295 +VNI D+LQ W++ S HRV + G R ++ +F D ++ L Sbjct: 219 VNVQPDVVLVNIADMLQFWTSGKLRSTVHRVRIDPGVKTRQTIAYFVTPDPETPLSPL-- 276 Query: 296 CCSAERPARYAPCTIGDYLEMKVKQTFGAVA 326 + T G++++ ++ T+G A Sbjct: 277 -FEEKTGKDIETVTAGEWIDGRINFTYGYSA 306 >RSp1529 [R] COG3491 Isopenicillin N synthase and related dioxygenases Rso Ralstonia solanacearum Length = 345 Score = 65.5 bits (158), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 77/290 (26%), Positives = 117/290 (40%), Gaps = 33/290 (11%) Query: 27 ARREALAWEIHKISRDVGFFYLVNHGVPQALIDGQFDWARRFFALPQASKSAIDMRHSAS 86 A RE L + K R G F + + D F +R+FF+ +KS + S Sbjct: 18 AHRE-LGQAMVKAWRTDGIFQITLSKPQEQTTDEAFAESRQFFSQDFETKSRHVSALTYS 76 Query: 87 GYGYERMGAQALDEGSPADLKEGFQFGFDIAPDHPYVQRGLLRYGHTLWPQDLPGFETHS 146 GY R A AD E F DI + V+ L +G WP + Sbjct: 77 GYIASREEVTA----GEADYSEIFTICPDIGLEDARVRENLPCHGPVPWPG--AAYRDRM 130 Query: 147 RQYYEAVRALSHRLLGVIALSLEMPE-DFFTPVLHTPIATQRMLHYPPQPASAQHNQIGA 205 + + + RLL +IAL L++ + D FT + R+L +P +S N G Sbjct: 131 KAFMGMLGTFGERLLQLIALGLDLDDMDTFTRLTQDGWHHMRVLRFPTVQSS--ENARGI 188 Query: 206 GAHTDWGLVTILAQDAIGGL------EICNAEGDWVSAPPIAGSF--------------- 244 GAHTD+G++ I AQD +GGL E +W+ + AG + Sbjct: 189 GAHTDYGMLVIAAQDDVGGLYVRPPIEGERRNRNWLPSESTAGVYEHDDGWNFIKPMPAV 248 Query: 245 -VVNIGDLLQRWSNDLYHSNAHRVCNVGNAPRYSLPFFQDGDQAAVVACL 293 V GD LQ + S H+V + R+++ +F + + A V L Sbjct: 249 LTVFPGDFLQFLTGGHLLSTPHKV-RLNTRERFAMAYFHEPNFDAWVEPL 297