BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0018.0069.p (265 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1981 [E] COG0159 Tryptophan synthase alpha chain Rso Ralstoni... 365 e-101 NMB0678 [E] COG0159 Tryptophan synthase alpha chain Nme Neisseri... 281 1e-75 NMA0879 [E] COG0159 Tryptophan synthase alpha chain NmA Neisseri... 277 2e-74 XF1376 [E] COG0159 Tryptophan synthase alpha chain Xfa Xylella f... 257 2e-68 PA0035 [E] COG0159 Tryptophan synthase alpha chain Pae Pseudomon... 236 3e-62 SMc02765 [E] COG0159 Tryptophan synthase alpha chain Sme Sinorhi... 211 2e-54 AGc30 [E] COG0159 Tryptophan synthase alpha chain Atu Agrobacter... 210 2e-54 mlr5073 [E] COG0159 Tryptophan synthase alpha chain Mlo Mesorhiz... 209 5e-54 BMEI2019 [E] COG0159 Tryptophan synthase alpha chain Bme Brucell... 197 2e-50 YGL026c_1 [E] COG0159 Tryptophan synthase alpha chain Sce Saccha... 197 2e-50 >RSc1981 [E] COG0159 Tryptophan synthase alpha chain Rso Ralstonia solanacearum Length = 265 Score = 365 bits (938), Expect = e-101, Method: Compositional matrix adjust. Identities = 180/265 (67%), Positives = 207/265 (78%) Query: 1 MSRIQTTFSQLAEQKRKALITFITAGDPAPELTVPLLHALVAGGVDILELGVPFSDPMAE 60 MSRI TFSQL+ Q RK LI FITAGDP PELTV L+HALV GG +++ELGVPFSDPMA+ Sbjct: 1 MSRIAQTFSQLSAQGRKGLIPFITAGDPYPELTVDLMHALVKGGANVIELGVPFSDPMAD 60 Query: 61 GPVIQRACERALKFGISTHDVLGYVREFRKTNTHTPIVLMGYANPIERFGVDAFIAAASE 120 GPVIQRA ERAL I VL YVR FR T+ TP+VLMGYANPIER G+DAF AASE Sbjct: 61 GPVIQRASERALAKKIGLRTVLDYVRAFRATDKTTPVVLMGYANPIERMGIDAFAKAASE 120 Query: 121 AGVDGTIVVDYPPEECEEFAQKMQAKGMDPIFLLAPTSSEERIRQVAAVSSGFCYYVSLK 180 AGVDG +VVDYPPEECE FA+ M+A G+DPIFLLAPTS+E R+ Q+A V+SG+ YYVSLK Sbjct: 121 AGVDGVLVVDYPPEECEAFAKTMRAAGIDPIFLLAPTSTEARMAQIARVASGYIYYVSLK 180 Query: 181 GVTGAGHIDTAEVAERIAAIRQHVKLPIAVGFGIRDGATARTVGSVADAVVIGSRIIAEL 240 GVTGA +D VA RI IRQH +LP+ VGFGIRD ATAR +G VADAVVIGSRI+ L Sbjct: 181 GVTGAATLDLDSVAARIPQIRQHARLPVGVGFGIRDAATARAIGGVADAVVIGSRIVQLL 240 Query: 241 ENTPQDQACDAVQAFVTDIRQALDA 265 E ++QA + F+ DIRQALDA Sbjct: 241 EEASREQAVQCLTDFIADIRQALDA 265 >NMB0678 [E] COG0159 Tryptophan synthase alpha chain Nme Neisseria meningitidis MC58 Length = 261 Score = 281 bits (719), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 138/263 (52%), Positives = 188/263 (71%), Gaps = 3/263 (1%) Query: 1 MSRIQTTFSQLAEQKRKALITFITAGDPAPELTVPLLHALVAGGVDILELGVPFSDPMAE 60 MSRI+ F+ L KALI +IT GDP T+ L+H +VA G DILELGVPFSDPMA+ Sbjct: 1 MSRIRQAFAAL--DGGKALIAYITVGDPDIRTTLALMHGMVANGADILELGVPFSDPMAD 58 Query: 61 GPVIQRACERALKFGISTHDVLGYVREFRKTNTHTPIVLMGYANPIERFGVDAFIAAASE 120 GPVIQRA ERAL GIS DVL VR+FR+T+T TP+VLMGY NP+ + G F A++ Sbjct: 59 GPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLNPVHKMGYREFAQEAAK 118 Query: 121 AGVDGTIVVDYPPEECEEFAQKMQAKGMDPIFLLAPTSSEERIRQVAAVSSGFCYYVSLK 180 AGVDG + VD P E + ++++ G+D IFL+APT++E+RI+ +A ++ GF YYVSLK Sbjct: 119 AGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDRIKTIAELAGGFVYYVSLK 178 Query: 181 GVTGAGHIDTAEVAERIAAIRQHVKLPIAVGFGIRDGATARTVGSVADAVVIGSRIIAEL 240 GVTGA +DT EV+ +I + Q++ +PI VGFGI + +AR +G VADAV++GSRI+ E+ Sbjct: 179 GVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESARKIGRVADAVIVGSRIVKEI 238 Query: 241 ENTPQDQACDAVQAFVTDIRQAL 263 EN ++A AV A V +++ A+ Sbjct: 239 ENNTGNEAA-AVGALVKELKDAV 260 >NMA0879 [E] COG0159 Tryptophan synthase alpha chain NmA Neisseria meningitidis Z2491 Length = 261 Score = 277 bits (708), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 136/263 (51%), Positives = 187/263 (71%), Gaps = 3/263 (1%) Query: 1 MSRIQTTFSQLAEQKRKALITFITAGDPAPELTVPLLHALVAGGVDILELGVPFSDPMAE 60 MSRI+ F+ L KALI +IT GDP T+ L+H +VA G DILELGVPFSDPMA+ Sbjct: 1 MSRIRQAFAAL--DGGKALIPYITVGDPDIRTTLALMHGMVANGADILELGVPFSDPMAD 58 Query: 61 GPVIQRACERALKFGISTHDVLGYVREFRKTNTHTPIVLMGYANPIERFGVDAFIAAASE 120 GPVIQRA ERAL GIS DVL VR+FR+T+T TP+VLMGY NP+ + G F A++ Sbjct: 59 GPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLNPVHKMGYREFAQEAAK 118 Query: 121 AGVDGTIVVDYPPEECEEFAQKMQAKGMDPIFLLAPTSSEERIRQVAAVSSGFCYYVSLK 180 AGVDG + VD P E + ++++ G+D IFL+APT++E+RI+ +A ++ GF YYVSLK Sbjct: 119 AGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDRIKTIAELAGGFVYYVSLK 178 Query: 181 GVTGAGHIDTAEVAERIAAIRQHVKLPIAVGFGIRDGATARTVGSVADAVVIGSRIIAEL 240 GVTGA +DT EV+ +I + Q++ +PI VGFGI + +AR +G VA+AV++GSRI+ E+ Sbjct: 179 GVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESARKIGRVAEAVIVGSRIVKEI 238 Query: 241 ENTPQDQACDAVQAFVTDIRQAL 263 EN ++A V A V +++ A+ Sbjct: 239 ENNAGNEAA-VVGALVKELKDAV 260 >XF1376 [E] COG0159 Tryptophan synthase alpha chain Xfa Xylella fastidiosa 9a5c Length = 268 Score = 257 bits (656), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 129/266 (48%), Positives = 173/266 (65%), Gaps = 1/266 (0%) Query: 1 MSRIQTTFSQLAEQKRKALITFITAGDPAPELTVPLLHALVAGGVDILELGVPFSDPMAE 60 M RI TF +L Q RKALI FITAGDP+ E VP++HALV G D++ELGVPFSDPMA+ Sbjct: 1 MHRIDETFRRLRAQSRKALIPFITAGDPSLEAAVPVMHALVRAGADVIELGVPFSDPMAD 60 Query: 61 GPVIQRACERALKFGISTHDVLGYVREFRKTNTHTPIVLMGYANPIERFGVDAFIAAASE 120 GPVIQ + ERAL+ G+ VL V FR+++ TP+VLMGY NP+E +G+ F A Sbjct: 61 GPVIQHSSERALQRGVGLAYVLQTVDVFRQSDAVTPVVLMGYLNPLEIYGIARFTQHAVA 120 Query: 121 AGVDGTIVVDYPPEECEEFAQKMQAKGMDPIFLLAPTSSEERIRQVAAVSSGFCYYVSLK 180 GVDG ++VD PPEE +E A G+ I L +PT+S R+ +++ V+ G+ YYVS Sbjct: 121 CGVDGVLLVDLPPEEADEIRPIFSAAGLALIVLASPTTSASRLARLSGVAQGYLYYVSFS 180 Query: 181 GVTGAGHIDTAEVAERIAAIRQHVKLPIAVGFGIRDGATARTVGSVADAVVIGSRIIAEL 240 GVTGA +D +R+ +R ++P+ VGFGIRD A+A + AD VV+GS ++ L Sbjct: 181 GVTGADRLDAQSAGDRLRGLRAQTQVPVVVGFGIRDAASAAVMAVDADGVVVGSALVTAL 240 Query: 241 ENTPQ-DQACDAVQAFVTDIRQALDA 265 + P D AC AF+ +RQALDA Sbjct: 241 SDAPDVDTACRRADAFLAPLRQALDA 266 >PA0035 [E] COG0159 Tryptophan synthase alpha chain Pae Pseudomonas aeruginosa Length = 268 Score = 236 bits (603), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 121/237 (51%), Positives = 155/237 (65%) Query: 1 MSRIQTTFSQLAEQKRKALITFITAGDPAPELTVPLLHALVAGGVDILELGVPFSDPMAE 60 MSR+QT F+QL ++ R AL+TF+TAGDP ++ +L L A G D++ELG+PF+DPMA+ Sbjct: 1 MSRLQTRFAQLKQENRAALVTFVTAGDPDYASSLEILKGLPAAGADVIELGMPFTDPMAD 60 Query: 61 GPVIQRACERALKFGISTHDVLGYVREFRKTNTHTPIVLMGYANPIERFGVDAFIAAASE 120 GP IQ A RAL G + L VREFR ++ TP+VLMGY NPI +GV+ FIA A E Sbjct: 61 GPAIQLANIRALDGGQTLARTLQMVREFRSGDSETPLVLMGYFNPIHHYGVERFIAEAKE 120 Query: 121 AGVDGTIVVDYPPEECEEFAQKMQAKGMDPIFLLAPTSSEERIRQVAAVSSGFCYYVSLK 180 GVDG IVVD PPE E+ QA G+D I L PT+ ++R+ V SSGF YYVS+ Sbjct: 121 VGVDGLIVVDLPPEHNEDLCHPAQAAGIDFIRLTTPTTGDQRLPTVLEGSSGFVYYVSVA 180 Query: 181 GVTGAGHIDTAEVAERIAAIRQHVKLPIAVGFGIRDGATARTVGSVADAVVIGSRII 237 GVTGA V E +A +R+H LPI +GFGIR A V +AD VV+GS +I Sbjct: 181 GVTGANAATLEHVEEAVARLRRHTDLPIGIGFGIRSAEHAAAVARLADGVVVGSALI 237 >SMc02765 [E] COG0159 Tryptophan synthase alpha chain Sme Sinorhizobium meliloti Length = 279 Score = 211 bits (536), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 108/242 (44%), Positives = 154/242 (63%) Query: 2 SRIQTTFSQLAEQKRKALITFITAGDPAPELTVPLLHALVAGGVDILELGVPFSDPMAEG 61 +R++ F+ +A + R L+T+ GDP + ++ ++ AL G DI+ELGVPFSDPMA+G Sbjct: 3 ARMEQRFADVAAEGRPVLVTYFMGGDPDFDTSLAIMKALRQAGADIIELGVPFSDPMADG 62 Query: 62 PVIQRACERALKFGISTHDVLGYVREFRKTNTHTPIVLMGYANPIERFGVDAFIAAASEA 121 P IQ A +RALK G S L R FR + TPIVLMGY NPI +GV+ F+A A EA Sbjct: 63 PAIQLAGQRALKAGQSLAKTLELARLFRAEDQRTPIVLMGYYNPIYIYGVERFLADALEA 122 Query: 122 GVDGTIVVDYPPEECEEFAQKMQAKGMDPIFLLAPTSSEERIRQVAAVSSGFCYYVSLKG 181 GVDG IVVD PPE +E KG+ I L PT+ + R+ +V +SGF YYVS+ G Sbjct: 123 GVDGLIVVDLPPEMDDELCIPALEKGISFIRLATPTTDDRRLPKVLENTSGFVYYVSMTG 182 Query: 182 VTGAGHIDTAEVAERIAAIRQHVKLPIAVGFGIRDGATARTVGSVADAVVIGSRIIAELE 241 +TG+ D + +A +A I+ H LP+ VGFG++ AR +G+ AD VV+G+ I+ ++ Sbjct: 183 ITGSALPDPSLIAGAVARIKAHTPLPVCVGFGVKTADHARAIGASADGVVVGTAIVNQIA 242 Query: 242 NT 243 ++ Sbjct: 243 SS 244 >AGc30 [E] COG0159 Tryptophan synthase alpha chain Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 298 Score = 210 bits (535), Expect = 2e-54, Method: Compositional matrix adjust. Identities = 110/261 (42%), Positives = 161/261 (61%), Gaps = 3/261 (1%) Query: 3 RIQTTFSQLAEQKRKALITFITAGDPAPELTVPLLHALVAGGVDILELGVPFSDPMAEGP 62 R+ F+ L + R ALIT+ GDP + ++ ++ AL G D++ELG+PFSDPMA+GP Sbjct: 23 RMDKRFADLRAENRPALITYFMGGDPDFQTSLGIMKALPEAGADVIELGMPFSDPMADGP 82 Query: 63 VIQRACERALKFGISTHDVLGYVREFRKTNTHTPIVLMGYANPIERFGVDAFIAAASEAG 122 IQ A +RALK G + L REFRK + TPIV+MGY NPI +GV+ F+ A +G Sbjct: 83 AIQLAGQRALKGGQTLKTTLDLAREFRKQDNATPIVMMGYYNPIYIYGVEKFLDDAIASG 142 Query: 123 VDGTIVVDYPPEECEEFAQKMQAKGMDPIFLLAPTSSEERIRQVAAVSSGFCYYVSLKGV 182 +DG IVVD PPE +E A+G++ I L PT+ +R+ V +SGF YYVS+ G+ Sbjct: 143 IDGLIVVDLPPEMDDELCIPALARGINFIRLATPTTDGKRLPAVLKNTSGFVYYVSMNGI 202 Query: 183 TGAGHIDTAEVAERIAAIRQHVKLPIAVGFGIRDGATARTVGSVADAVVIGSRIIAELEN 242 TG+ D + ++ + I+ H +LP+ VGFG++ A+ +G+VAD VV+GS I+ ++ Sbjct: 203 TGSALPDPSLISGAVGRIKAHTELPVCVGFGVKTADHAKAIGAVADGVVVGSAIVNQIAG 262 Query: 243 --TPQDQA-CDAVQAFVTDIR 260 T QA D V A T ++ Sbjct: 263 SLTKDGQATADTVPAVTTLVK 283 >mlr5073 [E] COG0159 Tryptophan synthase alpha chain Mlo Mesorhizobium loti Length = 279 Score = 209 bits (532), Expect = 5e-54, Method: Compositional matrix adjust. Identities = 106/241 (43%), Positives = 149/241 (61%) Query: 2 SRIQTTFSQLAEQKRKALITFITAGDPAPELTVPLLHALVAGGVDILELGVPFSDPMAEG 61 +RI ++L + R AL+T+ GDP + ++ ++ AL G DI+ELG+PFSDPMA+G Sbjct: 3 TRIDRRMAKLKTEGRPALVTYFMGGDPDYDTSLSIMKALPGAGSDIIELGMPFSDPMADG 62 Query: 62 PVIQRACERALKFGISTHDVLGYVREFRKTNTHTPIVLMGYANPIERFGVDAFIAAASEA 121 P IQ A RALK G + L EFR + TPIVLMGY NPI +GVD F+ A + Sbjct: 63 PAIQAAGLRALKGGQTLVKTLKMASEFRAGDNETPIVLMGYYNPIYIYGVDRFLKDALAS 122 Query: 122 GVDGTIVVDYPPEECEEFAQKMQAKGMDPIFLLAPTSSEERIRQVAAVSSGFCYYVSLKG 181 G+DG IVVD PPE EE G++ I L PT+ ++R+ +V +SGF YYVS+ G Sbjct: 123 GIDGLIVVDLPPEMDEELCIPALKAGINFIRLATPTTDDKRLPKVLQNTSGFVYYVSMTG 182 Query: 182 VTGAGHIDTAEVAERIAAIRQHVKLPIAVGFGIRDGATARTVGSVADAVVIGSRIIAELE 241 +TG+ DT +VA + I+ H LP+ VGFG++ AR +G+ AD VV+G+ I+ + Sbjct: 183 ITGSALADTGKVAAAVNRIKGHTDLPVCVGFGVKTAEQARVIGANADGVVVGTAIVNAVA 242 Query: 242 N 242 N Sbjct: 243 N 243 >BMEI2019 [E] COG0159 Tryptophan synthase alpha chain Bme Brucella melitensis Length = 303 Score = 197 bits (502), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 104/236 (44%), Positives = 144/236 (61%) Query: 2 SRIQTTFSQLAEQKRKALITFITAGDPAPELTVPLLHALVAGGVDILELGVPFSDPMAEG 61 +RI T F++L + R AL+T+ GDP E + ++ AL G D++ELG+PFSDPMA+G Sbjct: 27 TRIDTKFAELKAEGRPALVTYFMGGDPDLETALKVMKALPKAGADVIELGMPFSDPMADG 86 Query: 62 PVIQRACERALKFGISTHDVLGYVREFRKTNTHTPIVLMGYANPIERFGVDAFIAAASEA 121 P IQ A RAL G + L EFRK + TPIV+MGY N I +GV+ F+ A + Sbjct: 87 PAIQAAGLRALNAGQTLAKTLYMAAEFRKEDDTTPIVMMGYYNLIYIYGVERFLTDAKAS 146 Query: 122 GVDGTIVVDYPPEECEEFAQKMQAKGMDPIFLLAPTSSEERIRQVAAVSSGFCYYVSLKG 181 GVDG IVVD P E E G++ I L PT+ ++R+ +V SSGF YYVS+ G Sbjct: 147 GVDGLIVVDLPSEMDAELCIPAMKAGINFIRLTTPTTDDKRLPKVLHNSSGFVYYVSMNG 206 Query: 182 VTGAGHIDTAEVAERIAAIRQHVKLPIAVGFGIRDGATARTVGSVADAVVIGSRII 237 +TGA DTA+V E + I++ LPI VGFG++ A + + AD VV+G+ I+ Sbjct: 207 ITGAAIADTAKVGEAVRHIKKSTDLPICVGFGVKTPEQAAAIATHADGVVVGTAIV 262 >YGL026c_1 [E] COG0159 Tryptophan synthase alpha chain Sce Saccharomyces cerevisiae Length = 296 Score = 197 bits (502), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 102/258 (39%), Positives = 159/258 (61%), Gaps = 1/258 (0%) Query: 3 RIQTTFSQLAEQKRKALITFITAGDPAPELTVPLLHALVAGGVDILELGVPFSDPMAEGP 62 +++ TF+ ++ R AL+TF+TAG P + TVP+L GGVDI+ELG+PFSDP+A+GP Sbjct: 4 QLRQTFANAKKENRNALVTFMTAGYPTVKDTVPILKGFQDGGVDIIELGMPFSDPIADGP 63 Query: 63 VIQRACERALKFGISTHDVLGYVREFRKTNTHTPIVLMGYANPIERFGVDAFIAAASEAG 122 IQ + AL+ G++ L V + R PI+LMGY NPI +G + FI A++AG Sbjct: 64 TIQLSNTVALQNGVTLPQTLEMVSQARNEGVTVPIILMGYYNPILNYGEERFIQDAAKAG 123 Query: 123 VDGTIVVDYPPEECEEFAQKMQAKGMDPIFLLAPTSSEERIRQVAAVSSGFCYYVSLKGV 182 +G I+VD PPEE + + G+ I L+AP++++ER+ ++ ++ F Y VS G Sbjct: 124 ANGFIIVDLPPEEALKVRNYINDNGLSLIPLVAPSTTDERLELLSHIADSFVYVVSRMGT 183 Query: 183 TGAGHIDTAEVAERIAAIRQHVK-LPIAVGFGIRDGATARTVGSVADAVVIGSRIIAELE 241 TG +++ E I+ +R++ K P+AVGFG+ ++VGSVAD VVIGS+I+ Sbjct: 184 TGVQSSVASDLDELISRVRKYTKDTPLAVGFGVSTREHFQSVGSVADGVVIGSKIVTLCG 243 Query: 242 NTPQDQACDAVQAFVTDI 259 + P+ + D + +V I Sbjct: 244 DAPEGKRYDVAKEYVQGI 261