BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0017.0100.p (327 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0301 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 299 6e-81 VC1346 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 291 2e-78 SMc03207 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-... 262 1e-69 mlr8306 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 260 4e-69 CC2531 Non COG 241 1e-63 MT0966_1 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-... 107 5e-23 Rv0939_1 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-... 107 5e-23 AF0091 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-en... 68 3e-11 SSO2520 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-e... 67 7e-11 >RSc0301 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Rso Ralstonia solanacearum Length = 330 Score = 299 bits (765), Expect = 6e-81, Method: Compositional matrix adjust. Identities = 157/325 (48%), Positives = 203/325 (62%), Gaps = 2/325 (0%) Query: 1 MKLATLPDGSLDGALLLVSRDQRHAVPVPQIAASLLDAVQRWDQVQPLLQERYEALNQGG 60 MKLATL DGS DG L +VSRD + A IA +L A+ W P LQ YE LN G Sbjct: 1 MKLATLKDGSRDGQLAVVSRDLKTAHFATHIAGTLQRALDDWSFHAPQLQALYEQLNTGR 60 Query: 61 LTEAIAFDPRHCIAPLPRSPQWLDASAFLNHGRLMEEAFKTPPIPHFDTVPVMYQGASDD 120 F P +C+APLPR+ QW D SA+LNH L+ +A P F T P+MYQG SDD Sbjct: 61 ARHPFPFVPANCMAPLPRAYQWADGSAYLNHVELVRKARGAEMPPEFRTDPLMYQGGSDD 120 Query: 121 FLGPYQDVALPSEEDGIDFEGEFGVIVGPVPMGVQSSQALQAVRLLVQINDWSLRALGPH 180 GP+ + SE GIDFE E VI G VPMG + A +A+RL++ ND SLR L P Sbjct: 121 LAGPHDPIVCRSEAFGIDFEAEVAVITGDVPMGTGPAAAGEAIRLVMLANDVSLRNLIPA 180 Query: 181 EMKTGFGFLQAKPSTAFAPIAVTPDELGPAWRDGRVQMRLQVQWNGQPFGHPHGG-EMNF 239 E+ GFGF Q+KP+TAF+P+AVTPDELG AWR+ +V + + V WN + G P G +M F Sbjct: 181 ELAKGFGFFQSKPATAFSPVAVTPDELGDAWRERKVHLPMTVHWNSKKVGQPDCGIDMVF 240 Query: 240 SFGELIAHAARSRRLTAGTVIGSGTVSNQSRAAGSACIAERRVIEKIDHGDIRTGFMRFG 299 F +LIAH ++R + AG+++GSGT+SN R G CIAE+R++E ID G T FMR+G Sbjct: 241 DFSQLIAHICKTRNVRAGSIVGSGTISNADRGKGYCCIAEKRMLETIDAGAPVTEFMRYG 300 Query: 300 DEVSMQACFDDGRSGPFGLLQQRVV 324 D V ++ FD + FG + Q V+ Sbjct: 301 DTVKIEM-FDGNGASVFGAIDQEVM 324 >VC1346 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Vch Vibrio cholerae Length = 339 Score = 291 bits (744), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 156/335 (46%), Positives = 212/335 (63%), Gaps = 13/335 (3%) Query: 1 MKLATLPDGSLDGALLLVSRDQRHAVPVPQIAASLLDAVQRWDQVQPLLQERYEALNQGG 60 MKLATL +G DG L++VSRD V VP IA++L A+ WD V LQ+ Y ALN+ Sbjct: 1 MKLATLKNGMRDGQLVVVSRDLTLCVAVPDIASTLQQALDNWDSVVDSLQDVYFALNERT 60 Query: 61 LTEAIAFDPRHCIAPLPRSPQWLDASAFLNHGRLMEEAFKTPPIPHFDTVPVMYQGASDD 120 +T + FD HC++PLPR+ W D SA++NH L+ +A F T P+MYQG SD Sbjct: 61 ITARL-FDAEHCLSPLPRAYHWADGSAYVNHVELVRKARGAEMPASFWTDPLMYQGGSDT 119 Query: 121 FLGPYQDVALPSEEDGIDFEGEFGVIVGPVPMGVQSSQALQAVRLLVQINDWSLRALGPH 180 FL P + + E GIDFE E V+ V MG +A QA+RLL+ +ND SLR L P+ Sbjct: 120 FLAPRSPILMADEAWGIDFEAEVAVVTADVAMGSSVQEAEQAIRLLMLVNDVSLRGLIPN 179 Query: 181 EMKTGFGFLQAKPSTAFAPIAVTPDELGPAWRDGRVQMRLQVQWNGQPFGHPHGG-EMNF 239 E+ GFGF Q+KPS+AF+P+AVTPDELG AW R+ + L+ +N + FG P+ G +M F Sbjct: 180 ELAKGFGFYQSKPSSAFSPVAVTPDELGEAWDGARLALPLRTTYNHELFGCPNAGVDMTF 239 Query: 240 SFGELIAHAARSRRLTAGTVIGSGTVSNQSRA----------AGSACIAERRVIEKIDHG 289 SF ELIAHAA++R L AG++IGSGTVSN+ G +CIAE R+IE + G Sbjct: 240 SFPELIAHAAKTRALCAGSIIGSGTVSNKQGTDYGSAIREGGVGYSCIAELRMIETLRDG 299 Query: 290 DIRTGFMRFGDEVSMQACFDDGRSGPFGLLQQRVV 324 + +T FMRFGD V ++ + + FG ++Q+V+ Sbjct: 300 EPKTAFMRFGDTVRIE-MLNPQQQSIFGAIEQQVM 333 >SMc03207 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Sme Sinorhizobium meliloti Length = 338 Score = 262 bits (669), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 148/334 (44%), Positives = 201/334 (60%), Gaps = 13/334 (3%) Query: 1 MKLATLPDGSLDGALLLVSRDQRHAVPVPQIAASLLDAVQRWDQVQPLLQERYEALNQGG 60 MKLATL D + DG L++VS+D V IA +L A+ W P L+ E + G Sbjct: 1 MKLATLKDSTRDGKLVVVSKDLTRCSEVGHIARTLQAALDDWAHAGPRLERVAEGIETGA 60 Query: 61 LTEAIAFDPRHCIAPLPRSPQWLDASAFLNHGRLMEEAFKTPPIPHFDTVPVMYQGASDD 120 + F +PLPR+ QW D SA++NH L+ +A F T P++YQG SD Sbjct: 61 -QPTMRFHEHDAASPLPRAFQWADGSAYVNHVELVRKARNAEMPASFWTDPLIYQGGSDS 119 Query: 121 FLGPYQDVALPSEEDGIDFEGEFGVIVGPVPMGVQSSQALQAVRLLVQINDWSLRALGPH 180 FLGP + + + GID EGE VIV VPMG +A A+RL++ +ND SLR L P Sbjct: 120 FLGPRDPILMADDAWGIDMEGEAAVIVDDVPMGATLDEAKAAIRLVMLVNDVSLRGLIPG 179 Query: 181 EMKTGFGFLQAKPSTAFAPIAVTPDELGPAWRDGRVQMRLQVQWNGQPFGHPHGG-EMNF 239 E+ GFGF Q+KPS+AF+P+AVTP+ELG AW G++ + L V NG+PFG + G +M F Sbjct: 180 ELAKGFGFYQSKPSSAFSPVAVTPEELGEAWDGGKLHLPLHVDLNGEPFGRANAGIDMTF 239 Query: 240 SFGELIAHAARSRRLTAGTVIGSGTVSNQSR----------AAGSACIAERRVIEKIDHG 289 F +LI HAAR+R L+AGT+IGSGTVSN+ AG +CIAE R+IE I+ G Sbjct: 240 DFPQLIVHAARTRPLSAGTIIGSGTVSNKLEGGPGRPVSEGGAGYSCIAELRMIETIEGG 299 Query: 290 DIRTGFMRFGDEVSMQACFDDGRSGPFGLLQQRV 323 +T F++FGD V ++ G S FG ++Q+V Sbjct: 300 APKTQFLKFGDVVRIEMKDRTGHS-IFGAIEQKV 332 >mlr8306 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Mlo Mesorhizobium loti Length = 337 Score = 260 bits (664), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 149/334 (44%), Positives = 199/334 (59%), Gaps = 13/334 (3%) Query: 1 MKLATLPDGSLDGALLLVSRDQRHAVPVPQIAASLLDAVQRWDQVQPLLQERYEALNQGG 60 MKLATL DG+ DG L++VSRD +A +L A+ W +V P L E+L Sbjct: 1 MKLATLKDGTRDGKLVVVSRDLTQYTDASFLARTLQAALDDWRRVSPHLGTIAESLENNA 60 Query: 61 LTEAIAFDPRHCIAPLPRSPQWLDASAFLNHGRLMEEAFKTPPIPHFDTVPVMYQGASDD 120 + A F +PLPR+ QW D SA++NH L+ +A F T P++YQG SD Sbjct: 61 VPSA-RFHEHDAHSPLPRAYQWADGSAYVNHVELVRKARGVEMPASFWTDPLIYQGGSDS 119 Query: 121 FLGPYQDVALPSEEDGIDFEGEFGVIVGPVPMGVQSSQALQAVRLLVQINDWSLRALGPH 180 F+ P + + E GID E E VIV VPMG +A A+RL++ +ND +LRAL Sbjct: 120 FIAPRDPIRMADEAFGIDMEAEVAVIVDDVPMGASLEEARAAIRLVMLVNDVTLRALTGP 179 Query: 181 EMKTGFGFLQAKPSTAFAPIAVTPDELGPAWRDGRVQMRLQVQWNGQPFGHPHGG-EMNF 239 E+ GFGF Q+KPS+AF+P+AVTPDELG AW G+V + L NG+PFG + G +M F Sbjct: 180 ELAKGFGFFQSKPSSAFSPVAVTPDELGDAWDGGKVSLPLLADLNGKPFGRANTGVDMTF 239 Query: 240 SFGELIAHAARSRRLTAGTVIGSGTVSNQ----------SRAAGSACIAERRVIEKIDHG 289 F LI HAAR+R L AGT+IGSGTVSN+ + AG +CIAE R+IE I G Sbjct: 240 DFPALIVHAARTRPLAAGTIIGSGTVSNKLDGGPGKPVSAGGAGYSCIAELRMIETIASG 299 Query: 290 DIRTGFMRFGDEVSMQACFDDGRSGPFGLLQQRV 323 + +T F+RFGD V ++ G S FG ++Q+V Sbjct: 300 EPKTPFLRFGDTVRIEMKDSTGHS-IFGAIEQKV 332 >CC2531 Non COG Length = 335 Score = 241 bits (616), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 139/335 (41%), Positives = 197/335 (58%), Gaps = 15/335 (4%) Query: 1 MKLATLPDGSLDGALLLVSRDQRHAVPVPQIAASLLDAVQRWDQVQPLLQERYEALNQGG 60 MKLA+L G DG L++VS D IA +L A+ W++ P+L E+L G Sbjct: 1 MKLASLKGGR-DGRLVVVSNDLAWFTDAGTIAPTLQAALDNWERCGPMLAGLAESLEHGA 59 Query: 61 LTEAIAFDPRHCIAPLPRSPQWLDASAFLNHGRLMEEAFKTPPIPHFDTVPVMYQGASDD 120 + + F ++PLPR+ QW+D SA++NH +L+ +A F T P+MYQGASD Sbjct: 60 VPKE-RFHEHEALSPLPRAYQWVDGSAYVNHVQLVRKARGAEMPESFWTDPLMYQGASDG 118 Query: 121 FLGPYQDVALPSEEDGIDFEGEFGVIVGPVPMGVQSSQALQAVRLLVQINDWSLRALGPH 180 FL P + L G D EGE VIV VP+G +AL A+RL++ ND SLR L P Sbjct: 119 FLAPRDPIPLADASWGCDLEGEVAVIVDDVPLGATREEALAAIRLVMLCNDVSLRNLIPG 178 Query: 181 EMKTGFGFLQAKPSTAFAPIAVTPDELGPAWRDGRVQMRLQVQWNGQPFGHPHGG-EMNF 239 E+ GFGFLQ+KP++AF+P+AV+PD LG AW+ G++ L V+ +G+ FG G +M F Sbjct: 179 ELAKGFGFLQSKPASAFSPVAVSPDALGEAWKGGKLHGALLVELDGKDFGRADAGVDMTF 238 Query: 240 SFGELIAHAARSRRLTAGTVIGSGTVSNQSR-----------AAGSACIAERRVIEKIDH 288 FG L+AHAA++R L AGT++GSGTVSN+ G +C+AE R +E + H Sbjct: 239 DFGTLVAHAAKTRSLCAGTIVGSGTVSNRDADGGPGKPISEGGLGYSCLAEVRTVETLLH 298 Query: 289 GDIRTGFMRFGDEVSMQACFDDGRSGPFGLLQQRV 323 G +T F+ GD + ++ G S FG ++Q V Sbjct: 299 GAPKTPFLLGGDTIRIEMKDAKGHS-IFGAIEQTV 332 >MT0966_1 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) MtC Mycobacterium tuberculosis CDC1551 Length = 302 Score = 107 bits (266), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 78/245 (31%), Positives = 112/245 (45%), Gaps = 17/245 (6%) Query: 65 IAFDPRHCIAPLPRSPQWLDASAFLNHGRLMEEAFKTPPIPHFDT---VPVMYQGASDDF 121 +A D AP+PR P D+ FL+H R +EA + DT +P Y Sbjct: 57 VALDEVTLAAPIPRPPSIRDSLCFLDHMRNCQEAMGGGRV-LMDTWYRIPAFYFACPSTV 115 Query: 122 LGPYQDVALPSEEDGIDFEGEFGVIVGPVPMGVQSSQALQAVRLLVQINDWSLRALGPHE 181 LGPY D DFE E ++G + QA +++ NDWS R L E Sbjct: 116 LGPYDDAPTAPGSAWQDFELEIAAVIGTSGKDLTVEQAERSIIGYTIFNDWSARDLQMLE 175 Query: 182 MKTGFGFLQAKPS-TAFAPIAVTPDELGPAWRDGRVQMRLQVQWNGQPFGHPHGGEMNFS 240 + G + K S P VTPDEL P R G++ +R+ NG G +M++S Sbjct: 176 GQLRIGQAKGKDSGITLGPYLVTPDELEPYCRGGKLSLRVIALVNGTVIGSGSTAQMDWS 235 Query: 241 FGELIAHAARSRRLTAGTVIGSGTVSNQSRAAGSACIAERRVIEKIDHGDIRTGFMRFGD 300 FGE+IA+A+R LT G V GSGTV + ++E + + G++ GD Sbjct: 236 FGEVIAYASRGVTLTPGDVFGSGTVPTCT------------LVEHLRPPESFPGWLHDGD 283 Query: 301 EVSMQ 305 V++Q Sbjct: 284 VVTLQ 288 >Rv0939_1 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Mtu Mycobacterium tuberculosis H37Rv Length = 300 Score = 107 bits (266), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 78/245 (31%), Positives = 112/245 (45%), Gaps = 17/245 (6%) Query: 65 IAFDPRHCIAPLPRSPQWLDASAFLNHGRLMEEAFKTPPIPHFDT---VPVMYQGASDDF 121 +A D AP+PR P D+ FL+H R +EA + DT +P Y Sbjct: 57 VALDEVTLAAPIPRPPSIRDSLCFLDHMRNCQEAMGGGRV-LMDTWYRIPAFYFACPSTV 115 Query: 122 LGPYQDVALPSEEDGIDFEGEFGVIVGPVPMGVQSSQALQAVRLLVQINDWSLRALGPHE 181 LGPY D DFE E ++G + QA +++ NDWS R L E Sbjct: 116 LGPYDDAPTAPGSAWQDFELEIAAVIGTSGKDLTVEQAERSIIGYTIFNDWSARDLQMLE 175 Query: 182 MKTGFGFLQAKPS-TAFAPIAVTPDELGPAWRDGRVQMRLQVQWNGQPFGHPHGGEMNFS 240 + G + K S P VTPDEL P R G++ +R+ NG G +M++S Sbjct: 176 GQLRIGQAKGKDSGITLGPYLVTPDELEPYCRGGKLSLRVIALVNGTVIGSGSTAQMDWS 235 Query: 241 FGELIAHAARSRRLTAGTVIGSGTVSNQSRAAGSACIAERRVIEKIDHGDIRTGFMRFGD 300 FGE+IA+A+R LT G V GSGTV + ++E + + G++ GD Sbjct: 236 FGEVIAYASRGVTLTPGDVFGSGTVPTCT------------LVEHLRPPESFPGWLHDGD 283 Query: 301 EVSMQ 305 V++Q Sbjct: 284 VVTLQ 288 >AF0091 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Afu Archaeoglobus fulgidus Length = 324 Score = 67.8 bits (164), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 56/193 (29%), Positives = 91/193 (47%), Gaps = 10/193 (5%) Query: 77 PRSPQWL-DASAFLNHGRLMEEAFKTPPIPHFDTVPVMYQGASDDFLGPYQDVALPSEED 135 P P+ + D S F H R+ E + VPV Y+ +GP + V PS Sbjct: 101 PLKPKLMVDFSTFEEHVRMGFEKLGMSIPQEWYQVPVGYKMNPYSVIGPEEPVIWPSFTQ 160 Query: 136 GIDFEGEFGVIVGPVPMGVQSSQALQAVRLLVQINDWSLRALGPHEMKTG-FGFLQAK-- 192 +D+E EFG++VG V A+ V +ND S R + E+K G L++K Sbjct: 161 ILDYELEFGIVVGKRGKNVSKEDAMDYVLGYTIVNDISARDMEFIELKAMPLGPLKSKDF 220 Query: 193 -PSTAFAPIAVTPDELGPAWRDGRVQMRLQVQWNGQPFGHPHGGEMNFSFGELIAHAARS 251 +T P VT DE+ +D +++ + NG+ + +M++ +LIAH ++ Sbjct: 221 DNATPMGPCIVTKDEI----KDPH-NLKMVARVNGEVWSEASTKDMHWKIPDLIAHLSKD 275 Query: 252 RRLTAGTVIGSGT 264 + + GTV+ SGT Sbjct: 276 QTIHPGTVMLSGT 288 >SSO2520 [Q] COG0179 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway) Sso Sulfolobus solfataricus Length = 318 Score = 66.6 bits (161), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 55/197 (27%), Positives = 86/197 (43%), Gaps = 12/197 (6%) Query: 74 APLPRSPQWLDASAFLNHGRLMEEAFKT--PPIPH-FDTVPVMYQGASDDFLGPYQDVAL 130 APL R+ D AF H +E +K PIP + +P+ Y+G F G ++V Sbjct: 94 APLVRANMLRDFLAFRGH---VEATYKRRGQPIPEEWFRIPIYYKGDPAIFYGHLEEVPW 150 Query: 131 PSEEDGIDFEGEFGVIVGPVPMGVQSSQALQAVRLLVQINDWSLRALGPHEMKTGFGFLQ 190 P +D E E I+ + +A + ND+S R + EMK G + Sbjct: 151 PKYSKLVDIELEIAAIIYKRGKDIDKIRARDYILGFTIFNDFSARDIQMAEMKGLLGPAK 210 Query: 191 AKP-STAFAPIAVTPDELGPAWRDGRVQMRLQVQWNGQPFGHPHGGEMNFSFGELIAHAA 249 K + P VT DEL +R+ + N + + +M +SF ++IA+ + Sbjct: 211 GKDFANGLGPWIVTKDELSDI-----KGLRVYAKVNNEIWCDTKAEDMQWSFEDMIAYVS 265 Query: 250 RSRRLTAGTVIGSGTVS 266 + + G V GSGTVS Sbjct: 266 QDEYIRPGDVFGSGTVS 282