BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0016.0070.p (233 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0392 [NU] COG1317 Flagellar biosynthesis/type III secretory p... 122 9e-28 VC2131 [NU] COG1317 Flagellar biosynthesis/type III secretory pa... 96 9e-20 YPO1828 [NU] COG1317 Flagellar biosynthesis/type III secretory p... 90 4e-18 ZfliH [NU] COG1317 Flagellar biosynthesis/type III secretory pat... 89 8e-18 ECs2679 [NU] COG1317 Flagellar biosynthesis/type III secretory p... 89 8e-18 fliH [NU] COG1317 Flagellar biosynthesis/type III secretory path... 89 1e-17 STM1971 [NU] COG1317 Flagellar biosynthesis/type III secretory p... 86 6e-17 PA1103 [NU] COG1317 Flagellar biosynthesis/type III secretory pa... 65 1e-10 >RSp0392 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Rso Ralstonia solanacearum Length = 248 Score = 122 bits (305), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 66/180 (36%), Positives = 105/180 (58%), Gaps = 12/180 (6%) Query: 53 QLDAIREAARQEGFAEGREHGLQAGRSEGYVAGLQQGQQEVNEIIQHFRQIAVNFSTEVS 112 +L R+ R++GFA+G + G + GR +G ++ +++ ++Q R+ + Sbjct: 63 ELANARDEGRRDGFAQGFQDGFEQGRRQG-----EEDSRQIAALMQSVREA-------ID 110 Query: 113 QCSEKMAPELLDLALDLAKSMLKTALKARPELVLPTVSAAIQALPVLQLPASLRLHPEDA 172 Q + +A EL+ LAL LA+ L+ AL+A+PE +LP V + P PA LR+H +DA Sbjct: 111 QMNGSVAEELVGLALQLAQQFLRGALRAQPERILPLVREVLGDAPTAPAPAMLRVHADDA 170 Query: 173 ALVRKHLGEELAQQGWKIEEDALLDRGGCQVETRTNQIDATTATRWKRLGESLSKQLDWL 232 L+R+ LG ELA GW + DA ++RGGC+V+TR + DAT TRW L +L + W+ Sbjct: 171 ELIRQALGTELAAAGWTLVVDAAIERGGCRVQTRFGETDATLQTRWAELTRALGRDTAWI 230 >VC2131 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Vch Vibrio cholerae Length = 270 Score = 95.5 bits (236), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 67/243 (27%), Positives = 123/243 (50%), Gaps = 33/243 (13%) Query: 11 MTPYQRWELASFHDEPEP---------EPEPLPEEEEFLEEPPPV--PQLTAEQLDAIRE 59 +TP QRW L + E +P +P +EP V + + E++ IR Sbjct: 23 VTP-QRWGLPDYGAESNKAAKQTAFNYDPGWIPN----FDEPEQVVEHEFSEEEIALIRT 77 Query: 60 AARQEGF----AEGREHGLQAGRSEGYVAGLQQGQQE------------VNEIIQHFRQI 103 AA+QEGF AEG + G + G++EG+ AG Q+GQ + + E ++ F + Sbjct: 78 AAQQEGFEAGQAEGYQQGFEQGKAEGFQAGHQEGQTQGYQDGVAEGQALIQEQVKTFMAL 137 Query: 104 AVNFSTEVSQCSEKMAPELLDLALDLAKSMLKTALKARPELVLPTVSAAIQALPVLQLPA 163 A F+ + + ++ +L+D+ L L K ++ ++ P+++L TV A+++ALP+ Sbjct: 138 ANQFAQPLDLLNAQVEKQLVDMVLALTKEVVHVEVQTNPQVILDTVKASVEALPIAGHAI 197 Query: 164 SLRLHPEDAALVRKHLGE-ELAQQGWKIEEDALLDRGGCQVETRTNQIDATTATRWKRLG 222 +L+L+PED ++R+ GE E+ + W + + L RG Q+E + + R + + Sbjct: 198 TLKLNPEDVEIIRQAYGEQEIETRNWTLLSEPALSRGDVQIEAGESSVSYRMEERIRSVL 257 Query: 223 ESL 225 +S Sbjct: 258 KSF 260 >YPO1828 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Ype Yersinia pestis Length = 238 Score = 90.1 bits (222), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 66/226 (29%), Positives = 108/226 (47%), Gaps = 22/226 (9%) Query: 13 PYQRWELASFHDEPE-PEPEPLPE-----EEEFLEEPPPVPQ---LTAEQLDAIREAARQ 63 P+Q W L F + E P E +P+ E +E V + L QL+A ++ RQ Sbjct: 9 PWQPWSLKDFASQSEAPLSESMPDISLLFPNEPMEATAAVDEQQVLVNLQLEAEKQG-RQ 67 Query: 64 EGFAEGREHGLQAGRSEGYVAGLQQGQQEVNE--------IIQHFRQIAVNFSTEVSQCS 115 +GFA+G LQ G +GY GL++G Q+ + H++ + +F + Sbjct: 68 QGFAKG----LQEGLDKGYQTGLEEGHQQALADAQQQLAPMTAHWQVMVTDFQNTLDTLD 123 Query: 116 EKMAPELLDLALDLAKSMLKTALKARPELVLPTVSAAIQALPVLQLPASLRLHPEDAALV 175 +A L+ +AL AK ++ +L + IQ P+ LR++P+D A+V Sbjct: 124 SVIASRLVQIALAAAKQIIGQPAICDGTALLAQIQQMIQQEPMFAGKTQLRVNPDDLAIV 183 Query: 176 RKHLGEELAQQGWKIEEDALLDRGGCQVETRTNQIDATTATRWKRL 221 + LG L+ GW++ D+ + GGC+V +DA+ ATRW L Sbjct: 184 EQRLGSTLSLHGWRLLGDSQIHAGGCKVSAEEGDLDASLATRWHEL 229 >ZfliH [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein EcZ Escherichia coli O157:H7 EDL933 Length = 235 Score = 89.0 bits (219), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 69/230 (30%), Positives = 105/230 (45%), Gaps = 13/230 (5%) Query: 1 MRAKIIPKEEMTPYQRW---ELASFHDEPEPEPEPLPEEEEFL--EEPPPVPQLTAEQLD 55 M A IP + P++ W +LA P+ E P+ E EE + E P + Q A+ Sbjct: 1 MAAARIPMSDNLPWKTWTPDDLAP----PQAEFVPMVESEETIIEEAEPSLEQQLAQLQM 56 Query: 56 AIREAARQEGFAEGREHGLQAGRSEGYVAGLQQGQQEVNE----IIQHFRQIAVNFSTEV 111 E Q G AEGR+ G + G EG GL+QG E I +Q+ F T + Sbjct: 57 QAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTL 116 Query: 112 SQCSEKMAPELLDLALDLAKSMLKTALKARPELVLPTVSAAIQALPVLQLPASLRLHPED 171 +A L+ +AL+ A+ ++ ++ + +Q P+ LR+HP+D Sbjct: 117 DALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDD 176 Query: 172 AALVRKHLGEELAQQGWKIEEDALLDRGGCQVETRTNQIDATTATRWKRL 221 V LG L+ GW++ D L GGC+V +DA+ ATRW+ L Sbjct: 177 LQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQEL 226 >ECs2679 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Ecs Escherichia coli O157:H7 Length = 235 Score = 89.0 bits (219), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 69/230 (30%), Positives = 105/230 (45%), Gaps = 13/230 (5%) Query: 1 MRAKIIPKEEMTPYQRW---ELASFHDEPEPEPEPLPEEEEFL--EEPPPVPQLTAEQLD 55 M A IP + P++ W +LA P+ E P+ E EE + E P + Q A+ Sbjct: 1 MAAARIPMSDNLPWKTWTPDDLAP----PQAEFVPMVESEETIIEEAEPSLEQQLAQLQM 56 Query: 56 AIREAARQEGFAEGREHGLQAGRSEGYVAGLQQGQQEVNE----IIQHFRQIAVNFSTEV 111 E Q G AEGR+ G + G EG GL+QG E I +Q+ F T + Sbjct: 57 QAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTL 116 Query: 112 SQCSEKMAPELLDLALDLAKSMLKTALKARPELVLPTVSAAIQALPVLQLPASLRLHPED 171 +A L+ +AL+ A+ ++ ++ + +Q P+ LR+HP+D Sbjct: 117 DALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDD 176 Query: 172 AALVRKHLGEELAQQGWKIEEDALLDRGGCQVETRTNQIDATTATRWKRL 221 V LG L+ GW++ D L GGC+V +DA+ ATRW+ L Sbjct: 177 LQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQEL 226 >fliH [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Eco Escherichia coli K12 Length = 235 Score = 88.6 bits (218), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 70/229 (30%), Positives = 104/229 (45%), Gaps = 11/229 (4%) Query: 1 MRAKIIPKEEMTPYQRW---ELASFHDEPEPEPEPLPEEEEFLEEP-PPVPQLTAEQLDA 56 M A IP + P++ W +LA E P EP EE +EE P + Q A+ Sbjct: 1 MAAARIPMSDNLPWKTWTPDDLAPPQAEFVPIVEP---EETIIEEAEPSLEQQLAQLQMQ 57 Query: 57 IREAARQEGFAEGREHGLQAGRSEGYVAGLQQGQQEVNE----IIQHFRQIAVNFSTEVS 112 E Q G AEGR+ G + G EG GL+QG E I +Q+ F T + Sbjct: 58 AHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLVSEFQTTLD 117 Query: 113 QCSEKMAPELLDLALDLAKSMLKTALKARPELVLPTVSAAIQALPVLQLPASLRLHPEDA 172 +A L+ +AL+ A+ ++ ++ + +Q P+ LR+HP+D Sbjct: 118 ALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDL 177 Query: 173 ALVRKHLGEELAQQGWKIEEDALLDRGGCQVETRTNQIDATTATRWKRL 221 V LG L+ GW++ D L GGC+V +DA+ ATRW+ L Sbjct: 178 QRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQEL 226 >STM1971 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Sty Salmonella typhimurium LT2 Length = 235 Score = 85.9 bits (211), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 64/225 (28%), Positives = 103/225 (45%), Gaps = 20/225 (8%) Query: 13 PYQRWELASFHDEPEPEPE---PLPEEEEFLEEPPPVPQLTAEQ-----LDAIREAARQE 64 P+Q W D+ P PE P+ + L E P P+LTAEQ L ++ A ++ Sbjct: 6 PWQVWT----PDDLAPPPETFVPVEADNVTLTEDTPEPELTAEQQLEQELAQLKIQAHEQ 61 Query: 65 GFAEGREHGLQAGRSEGYVAGLQQGQQEVNE--------IIQHFRQIAVNFSTEVSQCSE 116 G+ G G Q G ++GY GL QG ++ I +Q+ F + Sbjct: 62 GYNAGLAEGRQKGHAQGYQEGLAQGLEQGQAQAQTQQAPIHARMQQLVSEFQNTLDALDS 121 Query: 117 KMAPELLDLALDLAKSMLKTALKARPELVLPTVSAAIQALPVLQLPASLRLHPEDAALVR 176 +A L+ +AL+ A+ ++ ++ + +Q P+ LR+HP+D V Sbjct: 122 VIASRLMQMALEAARQVIGQTPAVDNSALIKQIQQLLQQEPLFSGKPQLRVHPDDLQRVE 181 Query: 177 KHLGEELAQQGWKIEEDALLDRGGCQVETRTNQIDATTATRWKRL 221 + LG L+ GW++ D L GGC+V +DA+ ATRW+ L Sbjct: 182 EMLGATLSLHGWRLRGDPTLHHGGCKVSADEGDLDASVATRWQEL 226 >PA1103 [NU] COG1317 Flagellar biosynthesis/type III secretory pathway protein Pae Pseudomonas aeruginosa Length = 268 Score = 65.1 bits (157), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 64/243 (26%), Positives = 114/243 (46%), Gaps = 29/243 (11%) Query: 3 AKIIPKEEMTPYQRWELASFHDEPEPEP---------------EPLPEEEEFLE--EPPP 45 +++I +++ + W L SF DEP EP P EE LE +PP Sbjct: 12 SELIRGKDVAAFGLWSLPSF-DEPRDEPAVAAPQVPAVAEPAPAPPAVEEVELETVKPP- 69 Query: 46 VPQLTAEQLDAIREAARQEGFAEGREHGLQAGRSEGYVAGLQQGQQEVNEIIQHFRQIAV 105 T E+++AIR+ A EGFA G G AG+ + Q+ ++ + E +Q ++ Sbjct: 70 ----TLEEIEAIRQDAYNEGFATGERDGFHAGQ----LKARQEAEEALKERLQSLERLMT 121 Query: 106 NFSTEVSQCSEKMAPELLDLALDLAKSMLKTALKARPELVLPTVSAAIQALPVLQLPASL 165 +++ + +++L +A+ +++ L V + A++ LP+ A++ Sbjct: 122 QLLEPIAEQDALIEQGMVNLVNHVARQVIQRELHMDSSHVRQVLREALKLLPMG--AANI 179 Query: 166 RLHPEDAALVRKHLGEELAQQGWKIEEDALLDRGGCQVETRTNQIDATTATRWKRLGESL 225 R+H R E ++ W+I ED L GGC++ET ++IDAT TR + + L Sbjct: 180 RIHVNPQDFERVKALRERHEESWRILEDDSLLPGGCRIETEHSRIDATIETRLAQAVKQL 239 Query: 226 SKQ 228 +Q Sbjct: 240 FEQ 242