BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0040.p (168 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0322 [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 182 2e-46 PA3792 [E] COG0119 Isopropylmalate/homocitrate/citramalate synth... 177 1e-44 AGc4114 [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 155 4e-38 YOR108w [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 150 2e-36 SMc02717 [E] COG0119 Isopropylmalate/homocitrate/citramalate syn... 149 3e-36 Rv3710 [E] COG0119 Isopropylmalate/homocitrate/citramalate synth... 148 6e-36 MT3813 [E] COG0119 Isopropylmalate/homocitrate/citramalate synth... 148 7e-36 Cgl0250 [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 147 1e-35 mlr2792 [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 147 1e-35 YNL104c [E] COG0119 Isopropylmalate/homocitrate/citramalate synt... 144 1e-34 >RSp0322 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Rso Ralstonia solanacearum Length = 576 Score = 182 bits (463), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 86/142 (60%), Positives = 108/142 (76%) Query: 1 MLTDPSQKYRAIAPVDLPERRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHE 60 ML P+ KYR P+ LP+R WPS+TL +AP W+STDLRDGNQALFEPMN ERK+R+F Sbjct: 1 MLKHPATKYRPFPPIALPDRTWPSKTLTRAPIWMSTDLRDGNQALFEPMNAERKMRMFKM 60 Query: 61 LVRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGA 120 LV+IGFKEIE FPAAS+ D++ VR LI+ IPDDVT V+TQ R DLI T++S+ GA Sbjct: 61 LVQIGFKEIEAAFPAASQTDYDFVRELIEGGHIPDDVTIEVLTQAREDLIRRTMESLRGA 120 Query: 121 RRVIVHFYNAIAPAWREIVFSS 142 RR I+H YNA +P +R VF++ Sbjct: 121 RRAIIHVYNATSPVFRRTVFNT 142 >PA3792 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Pae Pseudomonas aeruginosa Length = 592 Score = 177 bits (448), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 81/157 (51%), Positives = 111/157 (70%) Query: 1 MLTDPSQKYRAIAPVDLPERRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHE 60 ML DPSQKYR + ++LP+R WPS+T+ + P W S+DLRDGNQ+L EPM+ +K+R F Sbjct: 39 MLKDPSQKYRPFSAINLPDRTWPSKTITEVPIWCSSDLRDGNQSLIEPMDAAKKMRFFKT 98 Query: 61 LVRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGA 120 LV++G K+IEV FP+AS DF VR LI+ + IPDDVT V+TQ R DLIT T +S+ GA Sbjct: 99 LVQVGLKQIEVAFPSASDTDFNFVRELIEGNHIPDDVTIQVLTQAREDLITRTFESLRGA 158 Query: 121 RRVIVHFYNAIAPAWREIVFSSRIASILNSRVYRARF 157 ++ IVH YNA AP++R IVF+ +++ A+ Sbjct: 159 KKAIVHVYNATAPSFRRIVFNQDKQGVVDIATNAAKL 195 >AGc4114 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 583 Score = 155 bits (392), Expect = 4e-38, Method: Composition-based stats. Identities = 67/146 (45%), Positives = 99/146 (67%) Query: 2 LTDPSQKYRAIAPVDLPERRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHEL 61 +TD S KYR +++P+R WP +T+ +AP W S DLRDGNQ+L PM +RK R+F L Sbjct: 26 MTDASVKYRPYPTINIPDRTWPGKTIDKAPIWCSVDLRDGNQSLVNPMGHDRKARMFKLL 85 Query: 62 VRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGAR 121 + +GFKEIE+GFP+AS+ DF+ R ++E +PDDV+ V+ Q R +LIT T +++ GA Sbjct: 86 LEMGFKEIEIGFPSASQTDFDFARWCVEEGNVPDDVSLQVLVQCRPELITRTFEALEGAN 145 Query: 122 RVIVHFYNAIAPAWREIVFSSRIASI 147 + I+HFYN+ + R +VF + I Sbjct: 146 KPIIHFYNSTSELQRRVVFGKDVHGI 171 >YOR108w [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Sce Saccharomyces cerevisiae Length = 604 Score = 150 bits (378), Expect = 2e-36, Method: Composition-based stats. Identities = 71/141 (50%), Positives = 98/141 (69%), Gaps = 2/141 (1%) Query: 1 MLTDPSQKYRAIAPVDLPERRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHE 60 ML DPS KYRA AP + +R WP +T+++AP WLSTDLRDGNQ+L +PM+ +K FH+ Sbjct: 30 MLRDPSVKYRAFAPPKMVKRIWPDKTIQKAPRWLSTDLRDGNQSLPDPMSVAQKKEYFHK 89 Query: 61 LVRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGA 120 L+ IGFKEIEV FP+AS+ DF+ R+ ++ PDDV + Q R LI TV+++ GA Sbjct: 90 LINIGFKEIEVSFPSASQTDFDFTRYAVEN--APDDVGIQCLVQSREHLIKRTVEALTGA 147 Query: 121 RRVIVHFYNAIAPAWREIVFS 141 +R +H Y A + +REIVF+ Sbjct: 148 KRATIHTYLATSDMFREIVFN 168 >SMc02717 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Sme Sinorhizobium meliloti Length = 569 Score = 149 bits (375), Expect = 3e-36, Method: Composition-based stats. Identities = 65/146 (44%), Positives = 98/146 (67%) Query: 2 LTDPSQKYRAIAPVDLPERRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHEL 61 + + + KY+ + LP+R WPS+ + +AP W S DLRDGNQAL +PM +RK R+FH L Sbjct: 12 MPEAAVKYQPYPQIVLPDRTWPSKAITEAPIWCSVDLRDGNQALVDPMGHDRKARMFHLL 71 Query: 62 VRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGAR 121 + +GFKEIE+GFP+AS+ D++ R ++E + +DV+ V+ Q R +LI T +++ GA Sbjct: 72 LDMGFKEIEIGFPSASQTDYDFARWCVEEGNVSEDVSLQVLVQCRPELIARTFEALEGAH 131 Query: 122 RVIVHFYNAIAPAWREIVFSSRIASI 147 R IVHFYN+ + R +VF +A I Sbjct: 132 RPIVHFYNSTSELQRRVVFGKDVAGI 157 >Rv3710 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Mtu Mycobacterium tuberculosis H37Rv Length = 644 Score = 148 bits (373), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 73/147 (49%), Positives = 99/147 (67%), Gaps = 4/147 (2%) Query: 5 PSQKYRAIA----PVDLPERRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHE 60 P +YR A P+ L R WP R + +AP W + DLRDGNQAL +PM+ RK R+F Sbjct: 42 PVNRYRPFAEEVEPIRLRNRTWPDRVIDRAPLWCAVDLRDGNQALIDPMSPARKRRMFDL 101 Query: 61 LVRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGA 120 LVR+G+KEIEVGFP+AS+ DF+ VR +I++ IPDDVT V+TQ R +LI T ++ +GA Sbjct: 102 LVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSGA 161 Query: 121 RRVIVHFYNAIAPAWREIVFSSRIASI 147 R IVHFYN+ + R +VF + A + Sbjct: 162 PRAIVHFYNSTSILQRRVVFRANRAEV 188 >MT3813 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases MtC Mycobacterium tuberculosis CDC1551 Length = 659 Score = 148 bits (373), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 73/147 (49%), Positives = 99/147 (67%), Gaps = 4/147 (2%) Query: 5 PSQKYRAIA----PVDLPERRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHE 60 P +YR A P+ L R WP R + +AP W + DLRDGNQAL +PM+ RK R+F Sbjct: 57 PVNRYRPFAEEVEPIRLRNRTWPDRVIDRAPLWCAVDLRDGNQALIDPMSPARKRRMFDL 116 Query: 61 LVRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGA 120 LVR+G+KEIEVGFP+AS+ DF+ VR +I++ IPDDVT V+TQ R +LI T ++ +GA Sbjct: 117 LVRMGYKEIEVGFPSASQTDFDFVREIIEQGAIPDDVTIQVLTQCRPELIERTFQACSGA 176 Query: 121 RRVIVHFYNAIAPAWREIVFSSRIASI 147 R IVHFYN+ + R +VF + A + Sbjct: 177 PRAIVHFYNSTSILQRRVVFRANRAEV 203 >Cgl0250 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Cgl Corynebacterium glutamicum Length = 616 Score = 147 bits (370), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 67/129 (51%), Positives = 92/129 (71%) Query: 12 IAPVDLPERRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHELVRIGFKEIEV 71 + + LP+R WP + + AP W + DLRDGNQAL +PM+ ERK R+F LV++GFKEIEV Sbjct: 48 VEDISLPDRTWPDKKITVAPQWCAVDLRDGNQALIDPMSPERKRRMFELLVQMGFKEIEV 107 Query: 72 GFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGARRVIVHFYNAI 131 GFP+AS+ DF+ VR +I++ +IPDDVT V+ Q R LI T ++ GA+ VIVHFYN+ Sbjct: 108 GFPSASQTDFDFVREIIEKGMIPDDVTIQVLVQAREHLIRRTFEACEGAKNVIVHFYNST 167 Query: 132 APAWREIVF 140 + R +VF Sbjct: 168 SILQRNVVF 176 >mlr2792 [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Mlo Mesorhizobium loti Length = 558 Score = 147 bits (370), Expect = 1e-35, Method: Composition-based stats. Identities = 67/146 (45%), Positives = 96/146 (65%) Query: 2 LTDPSQKYRAIAPVDLPERRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHEL 61 + D ++KY+ V L +R WP++ + +AP W S DLRDGNQAL +PM ERK R+F L Sbjct: 1 MPDAARKYQPYPTVGLTDRTWPNKVIDKAPIWCSVDLRDGNQALIDPMGHERKARMFGLL 60 Query: 62 VRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGAR 121 + +GFKEIE+GFP+AS+ DF+ R I+E +P DV+ V+ Q R +LIT T +++ GA Sbjct: 61 LDMGFKEIEIGFPSASQTDFDFARWCIEEGNVPADVSLQVLVQCRPELITRTFEALKGAT 120 Query: 122 RVIVHFYNAIAPAWREIVFSSRIASI 147 IVHFYN+ + R +VF + I Sbjct: 121 NPIVHFYNSTSELQRRVVFEKDVGGI 146 >YNL104c [E] COG0119 Isopropylmalate/homocitrate/citramalate synthases Sce Saccharomyces cerevisiae Length = 619 Score = 144 bits (362), Expect = 1e-34, Method: Composition-based stats. Identities = 68/141 (48%), Positives = 94/141 (66%), Gaps = 2/141 (1%) Query: 1 MLTDPSQKYRAIAPVDLPERRWPSRTLRQAPTWLSTDLRDGNQALFEPMNRERKLRLFHE 60 ML DPS KY+ L R+WP + +AP WLSTDLRDGNQ+L +PM+ E+K FH+ Sbjct: 31 MLKDPSSKYKPFNAPKLSNRKWPDNRITRAPRWLSTDLRDGNQSLPDPMSVEQKKEYFHK 90 Query: 61 LVRIGFKEIEVGFPAASRIDFETVRHLIDEHLIPDDVTPMVMTQLRADLITETVKSVAGA 120 LV IGFKEIEV FP+AS+ DF+ R+ ++ PDDV+ + Q R LI TV+++ GA Sbjct: 91 LVNIGFKEIEVSFPSASQTDFDFTRYAVEN--APDDVSIQCLVQSREHLIKRTVEALTGA 148 Query: 121 RRVIVHFYNAIAPAWREIVFS 141 ++ +H Y A + +REIVF+ Sbjct: 149 KKATIHTYLATSDMFREIVFN 169