BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0033.p (290 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA4108 [T] COG2206 HD-GYP domain Pae Pseudomonas aeruginosa 285 7e-77 RSc2515 [T] COG2206 HD-GYP domain Rso Ralstonia solanacearum 270 2e-72 RSc0592 [T] COG2206 HD-GYP domain Rso Ralstonia solanacearum 230 2e-60 AGc4164 [T] COG2206 HD-GYP domain Atu Agrobacterium tumefaciens ... 174 2e-43 CAC0475 [T] COG2206 HD-GYP domain Cac Clostridium acetobutylicum 161 1e-39 CAC0277 [T] COG2206 HD-GYP domain Cac Clostridium acetobutylicum 158 1e-38 CAC3650 [T] COG2206 HD-GYP domain Cac Clostridium acetobutylicum 142 7e-34 DR0719_2 [T] COG2206 HD-GYP domain Dra Deinococcus radiodurans 125 1e-28 BH3794 [T] COG2206 HD-GYP domain Bha Bacillus halodurans 119 8e-27 CAC3410 [T] COG2206 HD-GYP domain Cac Clostridium acetobutylicum 117 3e-26 >PA4108 [T] COG2206 HD-GYP domain Pae Pseudomonas aeruginosa Length = 414 Score = 285 bits (730), Expect = 7e-77, Method: Compositional matrix adjust. Identities = 135/254 (53%), Positives = 179/254 (70%) Query: 3 NCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLGMDDIACKE 62 + LV+EI+ SV R NALLS++RLK DEYTY+HS+AVC LM+ALAR L + D +E Sbjct: 125 HASDLVDEISASVLRHPNALLSLVRLKTSDEYTYMHSVAVCALMIALARQLELPDPLVRE 184 Query: 63 AGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDVCL 122 AGLAG LHD+GK +P ILNKP +L++ E+ +++RHP G L V V+DVCL Sbjct: 185 AGLAGLLHDIGKMAVPDPILNKPGKLTDPEFGLVRRHPQNGARMLLDCRQVSALVVDVCL 244 Query: 123 HHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLSRMASWQG 182 HHHE+IDG+GYP QIS+ +RM AVCDVYDA+TS+RPYK GW+ AE++ RMA W G Sbjct: 245 HHHERIDGTGYPFGLAQEQISLLARMGAVCDVYDAITSDRPYKKGWNAAEAIRRMAEWNG 304 Query: 183 HFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFVTTPSIEVVES 242 HFD +F AFVKA+GIYP+G++V+L+SGR +V+ Q+ L P VK F + S + Sbjct: 305 HFDPQVFRAFVKAVGIYPVGALVRLESGRLGVVLEQHGRSLLTPRVKVFFSARSKVPIPQ 364 Query: 243 EIIDLSAPGQTDAI 256 +++DL GQTD I Sbjct: 365 QVVDLGRAGQTDRI 378 >RSc2515 [T] COG2206 HD-GYP domain Rso Ralstonia solanacearum Length = 402 Score = 270 bits (691), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 133/263 (50%), Positives = 175/263 (66%), Gaps = 2/263 (0%) Query: 3 NCKSLVEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLGMDDIACKE 62 + + LVE I+ SV R AL+S+ RLK D YT+LHS+AVC LMVAL R LG+ D ++ Sbjct: 128 DAQPLVEAISGSVDRHPGALVSLARLKTKDRYTFLHSVAVCALMVALGRQLGLSDDEVRD 187 Query: 63 AGLAGYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDVCL 122 AGLAG LHD+GK IP E+LNKP L+ E++ + HP GYD L G + D LDVCL Sbjct: 188 AGLAGLLHDIGKIAIPPEVLNKPGALTTDEFRHVIGHPKAGYDILVGDPSMRDIALDVCL 247 Query: 123 HHHEKIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLSRMASWQG 182 HHHE++DG+GYP + G IS+++RM A+CDVYDAVTS+RPYK W+ A ++ RM W+G Sbjct: 248 HHHERMDGTGYPHKLAGENISLFARMGAICDVYDAVTSDRPYKKAWEAAYAIQRMVEWRG 307 Query: 183 -HFDRPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFVTTPSIEVVE 241 HFD +F AFVK++GIYP GS+V+L+SGR A+V+ L P VK F + S + Sbjct: 308 NHFDPMVFQAFVKSVGIYPTGSLVRLRSGRLAVVMEHASGALLSPTVKVFFSISSGTRIT 367 Query: 242 SEIIDLSAPGQTDAIAERSGQDW 264 E+IDL+ G E G DW Sbjct: 368 PEVIDLAQTGDQIVSREEPG-DW 389 >RSc0592 [T] COG2206 HD-GYP domain Rso Ralstonia solanacearum Length = 403 Score = 230 bits (587), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 110/249 (44%), Positives = 164/249 (65%), Gaps = 4/249 (1%) Query: 7 LVEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLGMDDIACKEAGLA 66 LV+ ++ S+AR+S AL+++ RLK D+YTYLHS AVC LMVALARTLG+ + +E GL Sbjct: 133 LVDNVSNSLARNSYALIALARLKNKDDYTYLHSFAVCALMVALARTLGLSEDEIRECGLG 192 Query: 67 GYLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHE 126 G +HD+GK+ +P +L+K L+++E ++Q H G+ L G + +VCLHHHE Sbjct: 193 GLVHDIGKSAMPRTLLDKSTALTKEELALLQTHAVGGHHLLIGTGQFSEIPREVCLHHHE 252 Query: 127 KIDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLS-RMASWQGHFD 185 +IDGSGYP +G++IS++++M A+CDVYD +TS PY W PA++L MA FD Sbjct: 253 RIDGSGYPDGQKGDEISLWAKMGAICDVYDTLTSSSPYHHAWSPAQALKYMMARTDSQFD 312 Query: 186 RPIFLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFVTTPSIEVVESEII 245 R +F AF +++GIYP+G++VKL++ R +V+ QN +P V F + + V E I Sbjct: 313 RTVFQAFTRSVGIYPVGTLVKLRTNRLGVVVHQNESSALRPDVVVFYSGNTKTRVRPERI 372 Query: 246 DLSAPGQTD 254 L G++D Sbjct: 373 SL---GRSD 378 >AGc4164 [T] COG2206 HD-GYP domain Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 315 Score = 174 bits (442), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 82/183 (44%), Positives = 115/183 (62%) Query: 10 EIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLGMDDIACKEAGLAGYL 69 +I+ ++ + + + RLK DE T++HS++V LM+ LG++ G++G L Sbjct: 122 KISDAIQANPAVFIGVTRLKSKDETTFVHSVSVSGLMILFGNYLGLNKETVNLLGVSGLL 181 Query: 70 HDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHEKID 129 HD+GK IP ILNK + LS +E +II HPA G D L +PD VLDVC +HHE+ID Sbjct: 182 HDIGKVEIPSSILNKTEGLSIEEREIINLHPAFGRDILSRDGQMPDMVLDVCFNHHERID 241 Query: 130 GSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLSRMASWQGHFDRPIF 189 G GYPS G +IS+++R+AA+CDVYDAVTS RPYK W ++L+ M GHFD + Sbjct: 242 GGGYPSGRAGGEISLHARIAAICDVYDAVTSVRPYKKPWTANDALTWMLRRDGHFDIQLL 301 Query: 190 LAF 192 F Sbjct: 302 KKF 304 >CAC0475 [T] COG2206 HD-GYP domain Cac Clostridium acetobutylicum Length = 351 Score = 161 bits (408), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 78/224 (34%), Positives = 128/224 (57%), Gaps = 1/224 (0%) Query: 8 VEEIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLGMDDIACKEAGLAG 67 V+E+ + ++ +K D+YTY+H + + L ++G++ K+ +A Sbjct: 99 VDELVDYIVDKGTVNTNLYEVKLYDDYTYIHCLDTGIMAAFLGLSMGLNTARIKDLSIAA 158 Query: 68 YLHDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHEK 127 LHD+GK IP EI+NK ++L++QEY II+ HPA G D L + + V+D +HHE+ Sbjct: 159 MLHDIGKTKIPSEIINKKEKLTDQEYAIIKNHPAYGRDILNSIGMLSEEVIDGVFNHHER 218 Query: 128 IDGSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLSRMASWQGHFDRP 187 DGSGYP +GN+IS + R+ ++CDV+ AV++ R Y+ + P E+ + S G P Sbjct: 219 YDGSGYPRGLKGNEISQFGRIISICDVFTAVSANRSYRLRFKPNEAYELILSGSGSMFDP 278 Query: 188 IFL-AFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKA 230 F+ F IYP+GS V+L +G V++QN +PV++ Sbjct: 279 DFVDEFRNTFFIYPLGSCVRLSNGVEGYVVKQNKSFPDRPVIRV 322 >CAC0277 [T] COG2206 HD-GYP domain Cac Clostridium acetobutylicum Length = 372 Score = 158 bits (400), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 86/235 (36%), Positives = 127/235 (54%), Gaps = 10/235 (4%) Query: 10 EIATSVARDSNALLSIIRLKRVDEYTYLHSIAVCTLMVALARTLGMDDIACKEAGLAGYL 69 EI + N ++ ++ +K D+YTY HS+ V + + + L + + L Sbjct: 115 EIIEEILSQKNVMIGLVDIKSFDDYTYQHSVNVAVISLIIGIKLKFHKYELFDLCIGALL 174 Query: 70 HDVGKAFIPLEILNKPDRLSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHEKID 129 HDVGK FIP EILNK L+E+E+KI++ H GYDYLKG + + L HHE+ Sbjct: 175 HDVGKMFIPSEILNKKGELTEEEFKIMKEHTVRGYDYLKGLYEISVPARIISLQHHERAG 234 Query: 130 GSGYPSRHQGNQISVYSRMAAVCDVYDAVTSERPYKSGWDPAESLSR-MASWQGHFDRPI 188 G GYP G +IS+ SR+ AV DVYDA+TS+R Y+ P E+L MA+ FD + Sbjct: 235 GQGYPEGRSGERISILSRIVAVADVYDAMTSDRVYRRAIAPNEALEYIMANSGSKFDYKV 294 Query: 189 FLAFVKALGIYPIGSIVKLQSGRTALVIRQNHVDLTKPVVKAFVTTPSIEVVESE 243 AF K++ YP GSIV+L + N + + + + F P I+++ SE Sbjct: 295 VQAFSKSVIPYPEGSIVRLSN---------NEIGVVTKIHEDFPLRPEIKIIYSE 340 >CAC3650 [T] COG2206 HD-GYP domain Cac Clostridium acetobutylicum Length = 355 Score = 142 bits (359), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 73/200 (36%), Positives = 119/200 (59%), Gaps = 3/200 (1%) Query: 32 DEYTYLHSIAVCTLMVALARTLGMDDIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQ 91 DEYT+ HS+ V + +A+ + + + +E AG LHD+GK I ILNKP RL+++ Sbjct: 135 DEYTFYHSVNVSFYGMLIAKWMNLSEKQIREVISAGLLHDLGKTKIKNSILNKPGRLTDE 194 Query: 92 EYKIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAV 151 E++ +++HP GY+ +K + D + +V L HHE+I+G+GYP + +++ +YSR+ A+ Sbjct: 195 EFEEMKKHPLYGYEIIKDDPTINDDIKNVILQHHERINGTGYPYGIKADEMGLYSRIIAI 254 Query: 152 CDVYDAVTSERPYKSGWDPAESLSRMASWQG--HFDRPIFLAFVKALGIYPIGSIVKLQS 209 DVYDA+TS+R YK P + M +G FD I F+ + +Y IG+ V L Sbjct: 255 ADVYDAMTSDRVYKKKKSPFRAF-EMFKTEGISIFDTDILRVFLNNINVYFIGAEVLLND 313 Query: 210 GRTALVIRQNHVDLTKPVVK 229 GR ++ +L KP++K Sbjct: 314 GRKGKIVYIPPNNLLKPILK 333 >DR0719_2 [T] COG2206 HD-GYP domain Dra Deinococcus radiodurans Length = 177 Score = 125 bits (314), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 66/163 (40%), Positives = 96/163 (58%), Gaps = 1/163 (0%) Query: 32 DEYTYLHSIAVCTLMVALARTLGMDDIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQ 91 D+ T H+ V L LA+ LG D+ + YLHD+GK IP IL+K L +Q Sbjct: 2 DDETGGHTARVVGLATRLAQRLGWDEEQVRALRWGAYLHDLGKIAIPDRILHKNGALDDQ 61 Query: 92 EYKIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAAV 151 E ++I+ H +GY+ L+ +P LD+ +HHE+ DG+GYPS +G+ I +R+ ++ Sbjct: 62 EKQVIRSHTTVGYEMLQDLHFLPAETLDLVRYHHERWDGTGYPSGLRGDNIPDAARIFSI 121 Query: 152 CDVYDAVTSERPYKSGWDPAESLSRMASWQG-HFDRPIFLAFV 193 DVYDA+TSERPYKS W P +L + + G HFD AF+ Sbjct: 122 VDVYDALTSERPYKSAWSPERALQEIRAGAGAHFDPQYVEAFL 164 >BH3794 [T] COG2206 HD-GYP domain Bha Bacillus halodurans Length = 231 Score = 119 bits (298), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 72/203 (35%), Positives = 109/203 (53%), Gaps = 1/203 (0%) Query: 28 LKRVDEYTYLHSIAVCTLMVALARTLGMDDIACKEAGLAGYLHDVGKAFIPLEILNKPDR 87 +K D+YTY H++ V L + L +D++ KE L LHD+GK IP ILNKP Sbjct: 4 IKAKDDYTYRHTVGVSILSRLIGTWLKLDEVILKELTLGAVLHDIGKVEIPDAILNKPGA 63 Query: 88 LSEQEYKIIQRHPALGYDYLKGFSGVPDYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSR 147 L++ EY+IIQ H GY ++ D V + L HHE+ DG+GYP + N+I + S+ Sbjct: 64 LTKDEYRIIQDHTVKGYRIIQESGIYSDTVALMALEHHEREDGTGYPYQKTRNEIHLLSK 123 Query: 148 MAAVCDVYDAVTSERPYKSGWDPAESLSRMASWQ-GHFDRPIFLAFVKALGIYPIGSIVK 206 + AV DV+ A+TS+R Y+ G + L +M + G + I FV+ IGS + Sbjct: 124 ILAVADVFHAMTSDRIYRKGMALYDVLLQMRQDRFGKLEPRITDFFVRRFMEQCIGSKAR 183 Query: 207 LQSGRTALVIRQNHVDLTKPVVK 229 L G ++ + + P+VK Sbjct: 184 LNDGSRVEIVFIPYENPICPIVK 206 >CAC3410 [T] COG2206 HD-GYP domain Cac Clostridium acetobutylicum Length = 265 Score = 117 bits (293), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 63/167 (37%), Positives = 95/167 (56%), Gaps = 2/167 (1%) Query: 32 DEYTYLHSIAVCTLMVALARTLGMDDIACKEAGLAGYLHDVGKAFIPLEILNKPDRLSEQ 91 D YT +HS +V +A L + C++ + LHD+GK I ILNKP +L+E Sbjct: 83 DNYTAVHSKSVAEYARFIAEKLKYSEEDCEKVFIGALLHDIGKIGISDTILNKPLKLTED 142 Query: 92 EYKIIQRHPALGYDYLKGFSGVP-DYVLDVCLHHHEKIDGSGYPSRHQGNQISVYSRMAA 150 EYK IQ HP +G+ L+ + +LD+ L+HHE+ DG GYPS +GN+I + + A Sbjct: 143 EYKTIQEHPQIGFSILELNEDFKENKILDMILYHHERYDGMGYPSGLKGNEIPQCASIVA 202 Query: 151 VCDVYDAVTSERPYKSGWDPAESLSRMASWQG-HFDRPIFLAFVKAL 196 V D +DA+TS R Y+ G D + ++ + G FD + AF++ L Sbjct: 203 VADAFDAMTSNRVYRKGKDRSFAVKEIIKNSGTQFDPQVVAAFIEYL 249