BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0083.p (376 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mlr5196 [R] COG0693 Putative intracellular protease/amidase Mlo ... 282 1e-75 CC0208 [R] COG0693 Putative intracellular protease/amidase Ccr C... 189 9e-48 CC2960 Non COG 82 2e-15 mll5195 [K] COG4977 Transcriptional regulator containing an amid... 80 7e-15 SMb20215 [K] COG4977 Transcriptional regulator containing an ami... 77 9e-14 mlr2766 [K] COG4977 Transcriptional regulator containing an amid... 75 3e-13 PA1850 [K] COG4977 Transcriptional regulator containing an amida... 70 7e-12 VNG2554H [R] COG0693 Putative intracellular protease/amidase Hbs... 67 9e-11 CC3367 Non COG 66 1e-10 PA5389 [K] COG4977 Transcriptional regulator containing an amida... 65 3e-10 >mlr5196 [R] COG0693 Putative intracellular protease/amidase Mlo Mesorhizobium loti Length = 353 Score = 282 bits (721), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 157/340 (46%), Positives = 215/340 (63%), Gaps = 10/340 (2%) Query: 30 SANADVAPPRPAKRKRPLVAIVADNIGTETTDLMIPHAILTRSGVADVVVVSPLAGSITL 89 A A +A + KR+RPL+AI+ N GTETTD ++P+ IL R+ VADVV ++ G + L Sbjct: 19 EAAATLAALKSPKRQRPLIAIIGINDGTETTDYLMPYGILRRADVADVVALATRPGPMRL 78 Query: 90 MPALSMTSQQTLDAFDAMHPEGADYVIVPAFHGEGDARIIAWIKKQFAGDAFIAGICEGA 149 PAL + T AFDA HP+GADYVIVPA + DA ++ WI+ Q A A + GIC GA Sbjct: 79 HPALQIEPDATTTAFDAEHPDGADYVIVPAMMRDDDADVLEWIRSQAAKGAMVIGICAGA 138 Query: 150 KVLGKAGLLDGRDATTHWYAIDDLRSAHPTLRWIRDRRYVVDRSVMTTTGVTASIPASLA 209 KV+G +GLLDG+ ATTHWY++ +LR HPT+ ++ DRR+VVD+ V TTTG+TAS+P L Sbjct: 139 KVVGASGLLDGKRATTHWYSVGELREKHPTIHYVADRRFVVDQGVATTTGITASMPMMLT 198 Query: 210 IVEAIAGAAAARRLAAQLGLAGYSAQHESAGFQLEARHLWRVIANRAAFYLHEEVRILVE 269 ++EAIAG A + LGLA + A+H+S F+ I N A HE++ I + Sbjct: 199 LIEAIAGRDKAEAVGRDLGLAHWDARHDSGAFKFTRPFALAAIGNTLAVLGHEQLGIPLT 258 Query: 270 PGVDGIALALTADPWSRTYRSQAVLIA-QQSEVMSQDGLLLHAQPVQAD--ADKNSKASG 326 G+D ++LAL AD WSRTYRS AV A + S ++S+DGL + + D D+ A+ Sbjct: 259 SGMDEVSLALVADAWSRTYRSGAVTFAGKDSAIVSRDGLRILPDQIATDWPVDRLVPAT- 317 Query: 327 LTLTLAGDIPPAAHLERSLGAISQRYGRSTADLVALQLEY 366 D PA L+++L IS R+G TAD VA+QLEY Sbjct: 318 ------ADPRPARALDQALQDISARHGARTADFVAMQLEY 351 >CC0208 [R] COG0693 Putative intracellular protease/amidase Ccr Caulobacter vibrioides Length = 329 Score = 189 bits (480), Expect = 9e-48, Method: Compositional matrix adjust. Identities = 128/324 (39%), Positives = 172/324 (53%), Gaps = 22/324 (6%) Query: 45 RPLVAIVADNIGTETTDLMIPHAILTRSGVADVVVVSPLAGSITLMPALSMTSQQTLDAF 104 +PLV I+AD G ETTDL+ P+A+L SG DV VV+P + LMP + QT A Sbjct: 20 KPLVVILADQRGVETTDLIAPYAVLAESGAVDVRVVAPTLAPVRLMPGQAWVRPQTTYAA 79 Query: 105 DAMHPEGADYVIVPAFHGEGDARIIAWIKKQFAGDAFIAGICEGAKVLGKAGLLDGRDAT 164 P D VIVP D AW++ Q A I IC GA+ L +AGLLDGR+AT Sbjct: 80 LTRRP---DVVIVPFLMPAYDPSRDAWLRAQAQAGARIVSICAGAETLARAGLLDGREAT 136 Query: 165 THWYAIDDLRSAHPTLRWIRDRRYVVDRSVMTTTGVTASIPASLAIVEAIAGAAAARRLA 224 THWY ++ L A P +RW RD+R++VD V TT GVTASIPASLA++ +AG R A Sbjct: 137 THWYELNRLAKAFPKVRWRRDQRWIVDGPVTTTAGVTASIPASLALLRDLAGDEVMRETA 196 Query: 225 AQLGLAGYSAQHESAGFQLEARHLWRVIANRAAFYLHEEVRILVEPGVDGIALALTADPW 284 +L L H+ A F++ +A + E+V + + G D +A A D W Sbjct: 197 TRLNLPLPVQAHDGAAFRVGTDAAMSGLAGWLNAWRAEKVSVRIADGFDDLAFATALDAW 256 Query: 285 SRTYRSQAVLIAQQSEVMSQDGLLL--HAQPVQADADKNSKASGLTLTLAGDIPPAAHLE 342 SRT RS+A A +S S+ GL + A+P +A + K T Sbjct: 257 SRTNRSEA--FASRS-ATSRHGLTIIAPAKPPRAARTIDPKDGDPTT------------- 300 Query: 343 RSLGAISQRYGRSTADLVALQLEY 366 +L AI+ YG++TA VAL LE+ Sbjct: 301 -ALKAIAAAYGKATARYVALTLEH 323 >CC2960 Non COG Length = 312 Score = 82.4 bits (202), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 70/224 (31%), Positives = 106/224 (47%), Gaps = 23/224 (10%) Query: 104 FDAMHPEGADYVIVPAFHGEGDARIIAWIKKQFAGDAFIAGICEGAKVLGKAGLLDGRDA 163 D + G D V VPA E +A++++ +A +C G VL +AGLLDG+ A Sbjct: 67 LDTLLIAGGDAVKVPASCPE----TLAFVRQAGRSARRVASVCSGTYVLAEAGLLDGKRA 122 Query: 164 TTHWYAIDDLRSAHPTLRWIRDRRYVVDRSVMTTTGVTASIPASLAIVEAIAGAAAARRL 223 TTHW D + +P +R DR ++ D +V ++ G+TA I SLA++ A G A ARR Sbjct: 123 TTHWSRTKDFQRRYPHVRLEPDRIWIQDGAVWSSAGITAGIDLSLALIGADEGEAVARRT 182 Query: 224 AAQLGLAGYSAQHESAGFQLEARHLWRVIANRAAFYLHEEVRILVEPGVDGIALALTADP 283 A QL H G Q + L + R L + L EP +++ AD Sbjct: 183 AQQL-----VVYHHRPGGQSQHSALIDLRPGRFDGLLSWARQNLSEP----LSVEQLADR 233 Query: 284 WSRTYRSQAVLIAQQSEVMSQDGL----------LLHAQPVQAD 317 + + R+ A L AQ++ V + LL +QP+Q + Sbjct: 234 AAMSPRNFARLFAQETGVTPAKAIERLRVEAARALLDSQPLQVE 277 >mll5195 [K] COG4977 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain Mlo Mesorhizobium loti Length = 325 Score = 80.1 bits (196), Expect = 7e-15, Method: Compositional matrix adjust. Identities = 43/100 (43%), Positives = 64/100 (64%), Gaps = 2/100 (2%) Query: 128 IIAWIKKQFAGDAFIAGICEGAKVLGKAGLLDGRDATTHWYAIDDLRSAHPTLRWIRDRR 187 I+AW+++ G A +C GA +L +AGLLDGR ATTHW++ D +P ++ +R Sbjct: 117 IVAWLRR--TGVRRTASVCTGAYLLAEAGLLDGRRATTHWWSTHDFARRYPAVKLDAERI 174 Query: 188 YVVDRSVMTTTGVTASIPASLAIVEAIAGAAAARRLAAQL 227 +V D SV T+ G++A I +LA++E G A ARR+A QL Sbjct: 175 FVRDGSVWTSAGISAGIDLALALIEDDLGPAIARRVAQQL 214 >SMb20215 [K] COG4977 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain Sme Sinorhizobium meliloti Length = 326 Score = 76.6 bits (187), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 61/170 (35%), Positives = 93/170 (54%), Gaps = 12/170 (7%) Query: 128 IIAWIKKQFAGDAFIAGICEGAKVLGKAGLLDGRDATTHWYAIDDLRSAHPTLRWIRDRR 187 ++ + ++ F +A C GA VL ++GLLDGR ATTHW L+ P ++ DR Sbjct: 94 LVEFARRSFETARRVAAPCTGAFVLAESGLLDGRRATTHWAFARQLQDRFPAVKVEEDRI 153 Query: 188 YVVDRSVMTTTGVTASIPASLAIVEAIAGAAAARRLAAQLGL----AGYSAQHESAGFQL 243 ++VD SV T+ G+TASI +LA+VE G AR +A +L + AG +Q SA +L Sbjct: 154 FIVDGSVWTSAGMTASIDLALAMVEKDYGQGVARSVARKLVVYHRRAGGQSQF-SALLEL 212 Query: 244 EARHLWRVIANRAAFYLHEEVR--ILVEPGVDGIALALTADPWSRTYRSQ 291 E + R+ R+ Y +R + VE D A L+A +SR +R++ Sbjct: 213 EPKS-DRI--QRSIDYAKANLRRGLSVEELAD--AAGLSARQFSRAFRAE 257 >mlr2766 [K] COG4977 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain Mlo Mesorhizobium loti Length = 328 Score = 75.1 bits (183), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 58/194 (29%), Positives = 93/194 (47%), Gaps = 15/194 (7%) Query: 46 PLVAIVADNIGTETTDLMIPHAILTRSGVA------DVVVVSPLAGSITLMPALSMTSQQ 99 P++A++A + G L +P + A + + + AG + L++ + Sbjct: 4 PIIAVLAFD-GISPFHLSVPCLVFGEDRTALGLPRFEFRICAVKAGPVHTEAGLTVAAPH 62 Query: 100 TLDAFDAMHPEGADYVIVPAFHGEGDA---RIIAWIKKQFAGDAFIAGICEGAKVLGKAG 156 L+ FD GAD VI+P++H G+ ++ +++ A I G+C G + AG Sbjct: 63 GLEGFD-----GADIVIIPSWHHLGEPPSPLLVEALRQAHRRGALIVGLCLGTFAIAAAG 117 Query: 157 LLDGRDATTHWYAIDDLRSAHPTLRWIRDRRYVVDRSVMTTTGVTASIPASLAIVEAIAG 216 LL GR A THW + L+ HP + YV + V+T+ GV A + L IV G Sbjct: 118 LLSGRGAATHWAYSERLQKLHPDIAVDATVLYVDNGDVVTSAGVAAGLDCCLHIVRVRYG 177 Query: 217 AAAARRLAAQLGLA 230 A AA RLA Q+ L+ Sbjct: 178 AEAALRLARQIVLS 191 >PA1850 [K] COG4977 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain Pae Pseudomonas aeruginosa Length = 334 Score = 70.1 bits (170), Expect = 7e-12, Method: Compositional matrix adjust. Identities = 51/145 (35%), Positives = 70/145 (48%), Gaps = 15/145 (10%) Query: 109 PEGADYVIVPAFHG----EGDARIIAWIKKQFAGDAFIAGICEGAKVLGKAGLLDGRDAT 164 PE D ++VP G +A ++AW+K + +C GA +L AG+LDGR AT Sbjct: 74 PESLDTLLVPGGLGVEAQTRNADLLAWLKAAEPCIRRLGSVCSGALILAAAGVLDGRPAT 133 Query: 165 THWYAIDDLRSAHPTLRWIRDRRYVVDRS-------VMTTTGVTASIPASLAIVEAIAG- 216 THW + LR P + DR + D + T+ GVTA I +LA+VEA G Sbjct: 134 THWADVATLRQGFPGVHVQGDRLHTYDPRCRDGDAHIFTSAGVTAGIDLALALVEADLGR 193 Query: 217 ---AAAARRLAAQLGLAGYSAQHES 238 + ARRL L G AQ + Sbjct: 194 SLALSVARRLVMFLKRPGGQAQFSA 218 >VNG2554H [R] COG0693 Putative intracellular protease/amidase Hbs Halobacterium sp. NRC-1 Length = 193 Score = 66.6 bits (161), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 41/91 (45%), Positives = 52/91 (57%), Gaps = 3/91 (3%) Query: 137 AGDAFIAGICEGAKVLGKAGLLDGRDATTHWYAIDDLRSAHPTLRWIRDRRYVVDRSVMT 196 G A IA +C GA +L AGLLDGR A TH A DDL +RD RYV D +V+T Sbjct: 100 GGGATIASVCTGAMLLADAGLLDGRPAATHASAADDLAEYDVD---VRDERYVDDGTVLT 156 Query: 197 TTGVTASIPASLAIVEAIAGAAAARRLAAQL 227 GVTA I +L +VE A A ++A ++ Sbjct: 157 AAGVTAGIDLALHVVERECSAGLAEQVATEI 187 >CC3367 Non COG Length = 324 Score = 66.2 bits (160), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 45/121 (37%), Positives = 62/121 (51%), Gaps = 5/121 (4%) Query: 111 GADYVIVPAFHGEGDARI----IAWIKKQFAGDAFIAGICEGAKVLGKAGLLDGRDATTH 166 GA ++ P + G DA + I ++ AG A + IC G VL GLLDGR ATTH Sbjct: 76 GASIIVAPGWRGL-DAPVPRPLIEALRAAHAGGARLMSICSGVFVLAATGLLDGRRATTH 134 Query: 167 WYAIDDLRSAHPTLRWIRDRRYVVDRSVMTTTGVTASIPASLAIVEAIAGAAAARRLAAQ 226 W D LR+ HP ++ D YV + V+T+ G A I L ++ G AA +A + Sbjct: 135 WRYADALRAHHPAIQVQPDVLYVDEGDVLTSAGSAAGIDLGLHLIRRDFGPEAANTVARR 194 Query: 227 L 227 L Sbjct: 195 L 195 >PA5389 [K] COG4977 Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain Pae Pseudomonas aeruginosa Length = 336 Score = 64.7 bits (156), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 44/151 (29%), Positives = 74/151 (49%), Gaps = 4/151 (2%) Query: 128 IIAWIKKQFAGDAFIAGICEGAKVLGKAGLLDGRDATTHWYAIDDLRSAHPTLRWIRDRR 187 ++ W++K + GI G+ VL +AGLLDGR AT HW AID + ++P L + Sbjct: 87 LVQWLRKLDRNGVTLGGIDTGSVVLAEAGLLDGRRATLHWEAIDAFQESYPQLS-VTQEL 145 Query: 188 YVVDRSVMTTTGVTASIPASLAIVEAIAGAAAARRLAAQLGLAGYSAQHESAGFQLEARH 247 + +D +T+ G TASI L ++ G A +++ Q L + + Q+ R+ Sbjct: 146 FEIDGPRITSAGGTASIDLMLDLIAQAHGPQLAVQVSEQFVLGRIRPRQDHQRLQVATRY 205 Query: 248 LWRVIANRAAFYLHEEVRILVEPGVDGIALA 278 ++NR + E+ EP + + LA Sbjct: 206 ---GVSNRKLVQVIGEMERHTEPPLTTLELA 233