BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0026.p (426 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value HI0874 [M] COG3307 Lipid A core - O-antigen ligase and related e... 130 7e-30 PM1863 [M] COG3307 Lipid A core - O-antigen ligase and related e... 126 1e-28 RSc2204 [M] COG3307 Lipid A core - O-antigen ligase and related ... 98 3e-20 VC0237 [M] COG3307 Lipid A core - O-antigen ligase and related e... 85 3e-16 XF1995 [M] COG3307 Lipid A core - O-antigen ligase and related e... 80 1e-14 mll7573 [M] COG3307 Lipid A core - O-antigen ligase and related ... 62 2e-09 >HI0874 [M] COG3307 Lipid A core - O-antigen ligase and related enzymes Hin Haemophilus influenzae Length = 399 Score = 130 bits (326), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 106/395 (26%), Positives = 190/395 (48%), Gaps = 4/395 (1%) Query: 19 MPFYSSLAVFLLVALSLVVPSGFSLGSLMLVLGAAVLLRKGKPRAILYREDWLIMLALLS 78 + F +L V L L++P G++ ++L + K + ED ++ + L Sbjct: 7 LTFTINLLVSLFFLTILIIPKGYNYAPIILSAIGLIYFIPLKKKLSFSSEDKKLIFSFLF 66 Query: 79 YFFVNAGLNLVHQAPGREYDAPLRFLLAIPALLLLRAYPPRPGALWTGAAIGAVGAGLLS 138 YFF ++++ RE D P R LL IP LLL + +P + + A+ G ++ Sbjct: 67 YFFTFLLSIIINKDGIREIDNPSRLLLFIPLLLLFKNFPIKRKTILYAIPSSALITGCVA 126 Query: 139 LWQWLADGAARPGGSTNPIQYGNIAALLAILATCGLARAIRLQQPLRWKLMHAAGLILGL 198 L+Q A G +P T IQ GNIA LA + + +++ + L +IL + Sbjct: 127 LFQKFALGYEKPFPETMHIQMGNIAISLATFSIV-ITLHFFIKKQYKSTLFGFVAIILAI 185 Query: 199 TASILSGSRGSWLAFPVCAVLAAWLLLRAGYSRLVLQMSAGSLTMLFCLALLPQSPLSAR 258 S LSG+RG W+ PV + +L +L++ + A L L P+ + R Sbjct: 186 MTSALSGARGGWIGLPVVVGIILFLYKEFINKKLIITLIAIITIGLTALITSPKFGIEKR 245 Query: 259 IRLAVVQTNGYVQRADADSSVGARLEMWRIGLHLAPQHLLAGWGKQGMMQAKHELVRQGL 318 A Y+++ + ++S+GAR +MW L + + G G G + +H+ V+ Sbjct: 246 YNAAKSDIVSYLEKNNRNTSLGARFDMWENALIAIKEAPIFGHGSDGYDEFRHKQVKSKQ 305 Query: 319 ASPSVDEHTHLHNEYLDALVKRGVPGLAAMLVLYLLPLWLFQRRQRLVTGSEARLAAIAG 378 + + LHN+YL++ VKRG+ G A++++ L P++ F + E + I G Sbjct: 306 MAKTTLNFGSLHNQYLESWVKRGLVGFIALILIILTPIFYFIKNLN-THNLETKCICILG 364 Query: 379 VMLVLSYLTFGLTQAFLTHNNGVMLY--AFLCSIL 411 ++ ++S++ + +Q+FL HN+G + Y A LC I+ Sbjct: 365 IIHIVSHIFYFTSQSFLAHNSGNIFYFSAMLCFIV 399 >PM1863 [M] COG3307 Lipid A core - O-antigen ligase and related enzymes Pmu Pasteurella multocida Length = 257 Score = 126 bits (316), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 70/225 (31%), Positives = 122/225 (54%), Gaps = 2/225 (0%) Query: 181 QQPLRWKLMHAAGLILGLTASILSGSRGSWLAFPVCAVLAAWLLLRAGYSRLVLQMSAGS 240 +Q R L + G+ S+LS +RG W+ P+ A + + +++ +S Sbjct: 17 RQQYRQMLWAIIATLFGILGSVLSTARGGWIGLPLLLCFALFAYRQYLSKKILFFVSLFF 76 Query: 241 LTMLFCLALLPQSPLSARIRLAVVQTNGYVQ-RADADSSVGARLEMWRIGLHLAPQHLLA 299 + +L +A P++ L +R+ A +T Y +A+ +S+GAR +W+ L +A + L Sbjct: 77 VFLLAAVAFAPKTKLISRVTEAYTETQQYFDAQAEQSTSIGARFALWKSALLMAKEKPLL 136 Query: 300 GWGKQGMMQAKHELVRQGLASPSVDEHTHLHNEYLDALVKRGVPGLAAMLVLYLLPLWLF 359 GWG G M+ + ++G+ S TH HN+YLD L K G+ GL A+L ++ +P F Sbjct: 137 GWGNAGAMEKRQVQQQEGMISEYAANFTHAHNQYLDNLSKYGLVGLVALLAIFSVPFCYF 196 Query: 360 QRRQRLVTGSEARLAAIAGVMLVLSYLTFGLTQAFLTHNNGVMLY 404 R++ + G E +L A G++ ++S + + LTQAFL HN+G + Y Sbjct: 197 YRKRHAIKGEE-KLVATLGMLHIISVMCYSLTQAFLNHNSGNIFY 240 >RSc2204 [M] COG3307 Lipid A core - O-antigen ligase and related enzymes Rso Ralstonia solanacearum Length = 435 Score = 98.2 bits (243), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 127/423 (30%), Positives = 193/423 (45%), Gaps = 24/423 (5%) Query: 21 FYSSLAVFLLVALSLVVPSGFSLGSLM-----LVLGAAVLLRKG-KPRAILYREDWLIML 74 +Y+ LAVFLL+ L V S +L+ L LGA V +R+ A D L+ L Sbjct: 15 WYAPLAVFLLIYPVLTVLKQGSASALLIAAGVLSLGAGVAVRRALSGPATPEGADRLVRL 74 Query: 75 ALLSYFFVNAGLNLVHQAPGR----EYDAPLRFLLAIPALLLLRAYPPRPGALWTGAAIG 130 + A + L G+ +DAP RFL A+P LLLR P R W + Sbjct: 75 TGWALCMPLAAVILSEVWHGQLRWNAFDAPARFLAAVPLFLLLRRAPLR-ALRWADGSFA 133 Query: 131 AVGAGLLSLWQWLAD--GAARPGGS-TNPIQYGNIAALLAILATCGLARAIRLQQPLRWK 187 A L + W A G R G + NPI +G+IA +L +L+ L+ + PL + Sbjct: 134 AGALAALGVGLWAARDWGEGRMGSAFLNPIHFGDIALILGVLSA--LSINWWRKDPLAVR 191 Query: 188 LMHAAGLILGLTASILSGSRGSWLAFPVCAVLAAWLLLRAGYSRL-VLQMSAGSLTMLFC 246 L+ GL+ GL AS+L+GSRG W+A P +L A R +R VL + L ++ Sbjct: 192 LLKVGGLLAGLVASLLTGSRGGWVALPFILLLVAVARGRDKPARWRVLVPAVAVLGVVVL 251 Query: 247 LALLPQSPLSARIRLAVVQTNGYVQRADADSSVGARLEMWRIGLHLAPQHLLAGWGKQGM 306 A+ + RI + V Y Q D+S+G RL++++ L L P+H + G G G Sbjct: 252 YAV--SGTIHERIHMVWVDLAQYAQ-GQKDTSIGIRLQIYQAALMLIPKHPIFGLGPGGF 308 Query: 307 MQAKHELVRQGLASPSVDE--HTHLHNEYLDALVKRGVPGLAAMLVLYLLPLWLFQRRQR 364 + LV G S + + HN+ L G+ G +++ ++L+P L R + Sbjct: 309 ADSLQALVDAGRMSATAAQLGRGEAHNQLLAYTANFGLVGGLSIVAIHLVPGLLCWRCLK 368 Query: 365 LVTGSEARLAAIAGVMLVLSYLTFGLTQAFLTHNNGVMLY-AFLCSILWAMSQEQPVSPS 423 + R AA+ G + L++ FGLT T Y A + S+ S P S + Sbjct: 369 AAA-APMRRAALMGAVFALAFFVFGLTVEIFTLKMTASFYAAVIASLAGIASHTGPASEA 427 Query: 424 NTA 426 T+ Sbjct: 428 GTS 430 >VC0237 [M] COG3307 Lipid A core - O-antigen ligase and related enzymes Vch Vibrio cholerae Length = 399 Score = 84.7 bits (208), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 97/403 (24%), Positives = 175/403 (43%), Gaps = 30/403 (7%) Query: 23 SSLAVFLLVALSLVVPSGFSLGSL-MLVLGAAVLLRKGKPRAILYREDWLIMLALLSYFF 81 + ++FL ++L L+ P GFS+ ++ +L L ++V L K L + D + ++ L +YF Sbjct: 6 TKTSIFLTISLLLITP-GFSVVAVGLLTLYSSVKLIKNGLN--LNKFDIIPLITLSAYFL 62 Query: 82 VNAGLNLVHQAPGREYDAPLRFLLAIPALLLLRAYPPR----PGALWTGAAIGAVGAGLL 137 N + ++ R DA +R LL IP ++ + L T + + GA Sbjct: 63 SNLPITIIDGDTLRYLDAGIRALLCIPMYFFIKNEISKGANLDNTLCTSTILASFGALAF 122 Query: 138 SLWQWLADGAARPGGSTNPIQYGNIAALLAILATCGLARAIRLQQPLRWKLMHAAGLILG 197 + +Q+ R G I +G +AA LAIL+ GL+ R+K ++ Sbjct: 123 AFYQFFILNMPRVDGFLFSINFGYLAAALAILS-FGLSFT-----QTRFKYYLYLSVVAA 176 Query: 198 LTASILSGSRGSWLAFPVCAVLAAWLLLRAGYSRLVLQMSAGSLTMLFCLALLPQSPLSA 257 A+IL+ +RG+ L +L + +R + L + S +L ++ + Sbjct: 177 TVATILTLTRGAILTLLFVFILFFIVNVRKIKFKQTLVFTLISF-LLVSVSYQFSPRIQE 235 Query: 258 RIRLAVVQTNGYV-QRADADSSVGARLEMWRIGLHLAPQHLLAGWGKQGMMQAKHELVRQ 316 R+ + + + A +S G RL++W + + + G EL ++ Sbjct: 236 RVDFTIFEISSIASNNIHAAASSGGRLQLWYAAVEAFKHNPIWGTTYSERESLNIELFKE 295 Query: 317 GLASPSVDEHT------HLHNEYLDALVKRGVPGLAAMLVLYLLPLWLFQRRQRLVTGSE 370 G VDE T H H++Y +A+ G G+ A+ + +LP +F R TGS Sbjct: 296 G----KVDEWTSTVPRGHAHSQYFEAIASNGTLGILAIFAMLILPFGVFLNDYR-KTGSP 350 Query: 371 ARLAAIAGVMLVLSYLTFGLTQAFLTHNNGVMLYAFLCSILWA 413 + G + ++ F LT+A L N Y F+ +I +A Sbjct: 351 ---ISQTGYLFAFGFIIFCLTEAPLQANLIGTFYGFMVAIFYA 390 >XF1995 [M] COG3307 Lipid A core - O-antigen ligase and related enzymes Xfa Xylella fastidiosa 9a5c Length = 423 Score = 79.7 bits (195), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 92/313 (29%), Positives = 138/313 (44%), Gaps = 13/313 (4%) Query: 95 REYDAPLRFLLAIPALLLLRAYPPRPGALWTGAAIGAVGAGLLSLWQWLADGAARPGGST 154 R+ D RFLL + L + A PR LW GAA+G G LL++ Q L G R G Sbjct: 102 RDLDIRTRFLLLPWSALWVYALRPRKQWLWCGAALGVFGVLLLAVIQIL-RGETRAEGWV 160 Query: 155 NPIQYGNIAALLAILATCGLARAIRLQQPLRWKLMHAAGLILGLTASILSGSRGSWLAFP 214 N I ++ L I+ +P + L+ + L+ +LSGSRG LA Sbjct: 161 NAIVLADVTMALTIMVV--------FARPKQSWLLPSLVLLACSAVIVLSGSRGVLLAMA 212 Query: 215 VCAVLAAWLLLRAGYSRLVLQMSAGSLTMLFCLALLPQSPLSARIRLAVVQTN-GYVQRA 273 V ++ + A +L ++ L +PQ ++ ++RL +Q + ++ Sbjct: 213 VVLLVLTMTVRWASLRVRLLLLAGLVLGGGALALEVPQ--VTEQMRLVELQADMRRLESG 270 Query: 274 DADSSVGARLEMWRIGLHLAPQHLLAGWGKQGMMQAKHEL-VRQGLASPSVDEHTHLHNE 332 D+DSS GARLE ++ L G G +A L V +G H HN+ Sbjct: 271 DSDSSTGARLERLQVAYATFLSKPLMGVGIGQFDRAMTLLPVCRGDEWLVRCHLRHAHND 330 Query: 333 YLDALVKRGVPGLAAMLVLYLLPLWLFQRRQRLVTGSEARLAAIAGVMLVLSYLTFGLTQ 392 + +GVPGL A+L +Y +P L R R A GVMLV +Y+ G+TQ Sbjct: 331 LAEWAATQGVPGLLAILAIYGVPFVLLLRLYAAGGRKSFRGPAATGVMLVAAYMLCGMTQ 390 Query: 393 AFLTHNNGVMLYA 405 + +H YA Sbjct: 391 SMFSHQMSTSFYA 403 >mll7573 [M] COG3307 Lipid A core - O-antigen ligase and related enzymes Mlo Mesorhizobium loti Length = 417 Score = 62.4 bits (150), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 76/296 (25%), Positives = 125/296 (42%), Gaps = 16/296 (5%) Query: 136 LLSLWQ--WLADGAARPGGSTNPIQYGNIAALLAILATCGLARAIRLQQPLRWKLMHAAG 193 LL+++Q WL G GG+ N I + + L ++ G I R L+ AA Sbjct: 102 LLAVFQQHWL--GMRAEGGAGNAIVFAEVLCLAVLVCVAGALSDIERH---RIALLCAA- 155 Query: 194 LILGLTASILSGSRGSWLAFPVCAVLAAWLLLRAGYSRLVLQMSAGSLTMLFCLALLPQS 253 + G A I SG+R WLA + + + R +++ L +A + Sbjct: 156 -LGGTIAIIYSGTRIVWLALLIAGIAVLLINQHRLKGRNAIRLLVLLLAAGAVIAAVGFQ 214 Query: 254 PLSARIRLAVVQTNGYVQRADADSSVGARLEMWRIGLHLAPQHLLAGWGKQGMMQAKHEL 313 +S R+ + D +++G RL +W IGL + L G G + A L Sbjct: 215 TISGRVDFLRSDLDALAVNGDHTTAIGLRLAVWDIGLKAFREMPLFGHG----VGATQAL 270 Query: 314 VRQGLASP-SVDE-HTHLHNEYLDALVKRGVPGLAAMLVLYLLPLWLFQRRQRLVTGSEA 371 ++QG +D H HN +L ALV+ G+ G + ++++ R T Sbjct: 271 IKQGFHDQFGMDAGFNHFHNGFLTALVQAGLLGAVTLAAMFVVAARNAAVVLRNSTDPIE 330 Query: 372 RLAAIAGVMLVLSYLTFGLTQAFLTHNN-GVMLYAFLCSILWAMSQEQPVSPSNTA 426 R A V++V++YLT G+T + H+ +L FL S + S Q P + A Sbjct: 331 RFGATMIVIMVITYLTSGMTGILIGHDILDSVLMVFLVSGTYLASGRQAPLPQDQA 386