BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0007.p (90 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc1369 [L] COG1573 Uracil-DNA glycosylase Rso Ralstonia solanac... 102 2e-22 aq_1693 [L] COG1573 Uracil-DNA glycosylase Aae Aquifex aeolicus 61 8e-10 BMEI1321 [L] COG1573 Uracil-DNA glycosylase Bme Brucella melitensis 60 2e-09 >RSc1369 [L] COG1573 Uracil-DNA glycosylase Rso Ralstonia solanacearum Length = 292 Score = 102 bits (255), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 51/85 (60%), Positives = 61/85 (71%), Gaps = 2/85 (2%) Query: 1 VASCLPYLQRQIELIQPTVLVALGKTAAISLLGLDPTTPVARLRGTVHRYQELPLVVTYH 60 VA C PYL+RQI LI+P V+V LG+ AA SLL TPV +LRG VH +P+VVTYH Sbjct: 204 VAMCDPYLKRQIALIKPRVIVVLGRFAAQSLL--QTQTPVGKLRGKVHEVDGVPVVVTYH 261 Query: 61 PAYLLRSLQDKSKTWADLCLAMSTY 85 PAYLLR+L DK++ W DLCLA Y Sbjct: 262 PAYLLRTLTDKARAWEDLCLARKVY 286 >aq_1693 [L] COG1573 Uracil-DNA glycosylase Aae Aquifex aeolicus Length = 195 Score = 60.8 bits (146), Expect = 8e-10, Method: Compositional matrix adjust. Identities = 30/79 (37%), Positives = 49/79 (62%), Gaps = 5/79 (6%) Query: 3 SCLPYLQRQIELIQPTVLVALGKTAAISLLGLDPTTPVARLRGTVHRYQELPLV---VTY 59 +C PYL+++IE+IQP V+ LG TA +LG + + ++RG V Y P + +TY Sbjct: 109 ACFPYLKKEIEIIQPKVICCLGATAGEGILG--KSLRITKVRGQVFPYPYNPRIKVFLTY 166 Query: 60 HPAYLLRSLQDKSKTWADL 78 HPAY+LR+ ++++ D Sbjct: 167 HPAYVLRNPKEETTIIKDF 185 >BMEI1321 [L] COG1573 Uracil-DNA glycosylase Bme Brucella melitensis Length = 290 Score = 59.7 bits (143), Expect = 2e-09, Method: Composition-based stats. Identities = 40/92 (43%), Positives = 52/92 (56%), Gaps = 8/92 (8%) Query: 4 CLPYLQRQIELIQPTVLVALGKTAAISLLGLDPTTPVARLRGT--VHRY---QELPLVVT 58 C P+++RQIEL P VLVALG A +L G + RLRG +HR E+P++ T Sbjct: 201 CRPFIERQIELAAPKVLVALGGPAGKALTG--AAEGILRLRGNWKIHRTPTGMEIPVMPT 258 Query: 59 YHPAYLLRSLQDKSKTWADLCLAMSTYQAQAR 90 HPAYLLR+ K W D LA+ A+ R Sbjct: 259 LHPAYLLRTPAQKRFAWRDF-LAVKLKLAELR 289