BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0212.p (368 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc2723 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouri... 581 e-165 NMB1556 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouri... 437 e-122 NMA1744 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouri... 437 e-122 YPO1638 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouri... 434 e-121 PA2626 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiourid... 422 e-118 STM1234 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouri... 421 e-117 ZycfB [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridy... 416 e-116 ECs1605 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouri... 416 e-116 trmU [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridyl... 416 e-116 VC1128 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiourid... 411 e-114 >RSc2723 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridylate) methyltransferase, contains the PP-loop ATPase domain Rso Ralstonia solanacearum Length = 370 Score = 581 bits (1497), Expect = e-165, Method: Compositional matrix adjust. Identities = 277/365 (75%), Positives = 315/365 (86%), Gaps = 8/365 (2%) Query: 1 MSKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVA 60 MS KRVV+GMSGGVDSSV+AWLLK+QGYEVIGLFMKNWEDDDDSEYCSTR+DWID VSVA Sbjct: 1 MSGKRVVVGMSGGVDSSVTAWLLKQQGYEVIGLFMKNWEDDDDSEYCSTRQDWIDVVSVA 60 Query: 61 DVVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADL 120 D++GVD+EAVNFA+EYKDRVFA+FLREY AGRTPNPDVLCNAEIKFKAFLDHAM LGAD Sbjct: 61 DLIGVDVEAVNFAAEYKDRVFADFLREYSAGRTPNPDVLCNAEIKFKAFLDHAMALGADT 120 Query: 121 IATGHYARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRRIA 180 IATGHYARVR+ G F+LLKA D +KDQSYFLHRLNQ QLSRT+FPLGE+ KT VR IA Sbjct: 121 IATGHYARVREA-GGRFELLKAFDHTKDQSYFLHRLNQAQLSRTLFPLGEMPKTRVREIA 179 Query: 181 EQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQ 240 ++ LPNA+KKDSTGICFIGERPFR+FLNRYL KPGPI+T DG+T+G+H+GL+FYTLGQ Sbjct: 180 AEIGLPNARKKDSTGICFIGERPFRDFLNRYLPTKPGPIRTPDGKTIGQHIGLAFYTLGQ 239 Query: 241 RKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQVSWVAG 300 RKGIGIGG + DG+GDAWYVARKD+ NTLY+ QGHDHPWLL+ T+ A +SWVAG Sbjct: 240 RKGIGIGGSR-----DGNGDAWYVARKDMAANTLYVAQGHDHPWLLAHTVHADDLSWVAG 294 Query: 301 HAP-QMAALSAKTRYRQADVPCGFSDA-GASFSLAFDTPQWAVTPGQSAVLYDGDVCLGG 358 H P + L+AKTRYRQAD PC + A G + +L F QWAVTPGQSAVLYDGD+CLGG Sbjct: 295 HPPAEGTQLAAKTRYRQADAPCAVTRATGDALTLTFQQAQWAVTPGQSAVLYDGDICLGG 354 Query: 359 GIIET 363 GII + Sbjct: 355 GIIAS 359 >NMB1556 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridylate) methyltransferase, contains the PP-loop ATPase domain Nme Neisseria meningitidis MC58 Length = 367 Score = 437 bits (1125), Expect = e-122, Method: Compositional matrix adjust. Identities = 221/365 (60%), Positives = 271/365 (74%), Gaps = 15/365 (4%) Query: 6 VVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVADVVGV 65 +++G+SGGVDSSV+A LLK+QGY+V G+FM+NWEDDD+ EYCS ++D DA++VAD++G+ Sbjct: 10 IIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWEDDDNDEYCSIKQDSFDAIAVADIIGI 69 Query: 66 DIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADLIATGH 125 DI+ VNFA++YKD+VFA FL+EY AGRTPNPDVLCNAEIKFK FLD+A+ GAD IATGH Sbjct: 70 DIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDVLCNAEIKFKCFLDYAVGQGADTIATGH 129 Query: 126 YARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRRIAEQLQL 185 YAR ++ G LLK LD +KDQSYFL+RL QL R +FPLG + K EVRR+A + +L Sbjct: 130 YAR-KEVRNGVHYLLKGLDRNKDQSYFLYRLKPFQLERAIFPLGGLEKPEVRRLAAEFKL 188 Query: 186 PNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQRKGIG 245 P A KKDSTGICFIGERPFREFL +YL G + T +G+TVGEHVGL FYTLGQRKG+G Sbjct: 189 PTAAKKDSTGICFIGERPFREFLQKYLPTDNGKMVTPEGKTVGEHVGLMFYTLGQRKGLG 248 Query: 246 IGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQVSWVAGHAPQM 305 IG G G+ W+VA KD+ N L +VQGHDHP L + +L +S+ P+ Sbjct: 249 IG---------GAGEPWFVAAKDLTKNELIVVQGHDHPLLYTRSLVMNDLSFTLPERPKA 299 Query: 306 AALSAKTRYRQADVPCG---FSDAGASFSLAFDTPQWAVTPGQSAVLYDGDVCLGGGIIE 362 + KTRYR AD PC D A L FD PQWAVTPGQSAVLYDGD+CLGGGII+ Sbjct: 300 GRYTCKTRYRMADAPCELRYLDDETA--ELVFDEPQWAVTPGQSAVLYDGDICLGGGIIQ 357 Query: 363 TGALP 367 T P Sbjct: 358 TTDKP 362 >NMA1744 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridylate) methyltransferase, contains the PP-loop ATPase domain NmA Neisseria meningitidis Z2491 Length = 383 Score = 437 bits (1125), Expect = e-122, Method: Compositional matrix adjust. Identities = 215/363 (59%), Positives = 264/363 (72%), Gaps = 11/363 (3%) Query: 6 VVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVADVVGV 65 +++G+SGGVDSSV+A LLK+QGY+V G+FM+NWEDDD+ EYCS ++D DA++VAD+VG+ Sbjct: 26 IIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWEDDDNDEYCSIKQDSFDAIAVADIVGI 85 Query: 66 DIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADLIATGH 125 DI+ VNFA++YKD+VFA FL+EY AGRTPNPDVLCNAEIKFK FLD+A+ GAD IATGH Sbjct: 86 DIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDVLCNAEIKFKCFLDYAVGQGADTIATGH 145 Query: 126 YARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRRIAEQLQL 185 YAR ++ G LLK LD +KDQSYFL+RL QL R +FPLG + K EVRR+A + L Sbjct: 146 YAR-KEVRNGVHYLLKGLDQNKDQSYFLYRLKPFQLERAIFPLGGLEKPEVRRLAAEFNL 204 Query: 186 PNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQRKGIG 245 P A KKDSTGICFIGERPFREFL +YL G + T +G+TVGEHVGL FYTLGQRK Sbjct: 205 PTAAKKDSTGICFIGERPFREFLQKYLPTDNGKMVTPEGKTVGEHVGLMFYTLGQRK--- 261 Query: 246 IGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQVSWVAGHAPQM 305 G G+ W+VA KD+ N L +VQGHDHP L + +L +S+ P+ Sbjct: 262 ------GLGIGGGGEPWFVAAKDLTKNELMVVQGHDHPLLYTRSLVMNDLSFTLPERPKE 315 Query: 306 AALSAKTRYRQADVPCGFSDA-GASFSLAFDTPQWAVTPGQSAVLYDGDVCLGGGIIETG 364 + KTRYR AD PC + L FD PQWAVTPGQSAVLYDGD+CLGGGII++ Sbjct: 316 GHYTCKTRYRMADAPCELRYLDDETVELVFDEPQWAVTPGQSAVLYDGDICLGGGIIQST 375 Query: 365 ALP 367 P Sbjct: 376 DKP 378 >YPO1638 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridylate) methyltransferase, contains the PP-loop ATPase domain Ype Yersinia pestis Length = 371 Score = 434 bits (1117), Expect = e-121, Method: Compositional matrix adjust. Identities = 220/365 (60%), Positives = 270/365 (73%), Gaps = 11/365 (3%) Query: 2 SKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVAD 61 S+K+V++GMSGGVDSSVSA+LL++QGY+V GLFMKNWE+DDD EYCS D DA +V D Sbjct: 5 SQKKVIVGMSGGVDSSVSAYLLQQQGYQVAGLFMKNWEEDDDEEYCSAATDLADAQAVCD 64 Query: 62 VVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMK-LGADL 120 +G+++ VNFA+EY D VF FL EY+AGRTPNPD+LCN EIKFKAFL+ A + LGAD Sbjct: 65 KLGMELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILCNKEIKFKAFLEFAAEDLGADY 124 Query: 121 IATGHYARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRRIA 180 IATGHY R RQ G +LL+ LD +KDQSYFL+ L+ EQ+++++FP+GE+ K EVRRIA Sbjct: 125 IATGHYVR-RQDVDGKSRLLRGLDGNKDQSYFLYTLSHEQIAQSLFPVGELEKPEVRRIA 183 Query: 181 EQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQ 240 EQL L AKKKDSTGICFIGER FR+FL RYL +PGPI T DGQ VG+H GL ++TLGQ Sbjct: 184 EQLDLVTAKKKDSTGICFIGERKFRDFLGRYLPAQPGPIMTVDGQLVGKHQGLMYHTLGQ 243 Query: 241 RKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQVSWVAG 300 RKG+GIGG K +G D WYV KD+++NTL + QGH+HP L+S L A Q+ WV Sbjct: 244 RKGLGIGGTK-----EGGDDPWYVVDKDLDSNTLLVAQGHEHPRLMSVGLVAQQLHWV-D 297 Query: 301 HAPQMAALSA--KTRYRQADVPCGFSDA-GASFSLAFDTPQWAVTPGQSAVLYDGDVCLG 357 P A KTRYRQ D+PC + + FD P AVTPGQSAV Y G++CLG Sbjct: 298 RQPVTAPFRCVVKTRYRQQDIPCTVTPLDDERVDVRFDDPVAAVTPGQSAVFYQGEICLG 357 Query: 358 GGIIE 362 GGIIE Sbjct: 358 GGIIE 362 >PA2626 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridylate) methyltransferase, contains the PP-loop ATPase domain Pae Pseudomonas aeruginosa Length = 375 Score = 422 bits (1085), Expect = e-118, Method: Compositional matrix adjust. Identities = 208/362 (57%), Positives = 261/362 (72%), Gaps = 7/362 (1%) Query: 3 KKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVADV 62 ++RV++GMSGGVDSSVSA LL +QGY+V GLFMKNW++DD +EYC+ RED DA +V D Sbjct: 10 RERVIVGMSGGVDSSVSALLLLQQGYQVEGLFMKNWDEDDGTEYCTAREDLADAQAVCDR 69 Query: 63 VGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMKLGADLIA 122 +G+ + NFA+EY D VF FL EY+AGRTPNPD+LCN EIKFKAFLD+A+ LGADLIA Sbjct: 70 IGIKLHTANFAAEYWDNVFEHFLAEYKAGRTPNPDILCNREIKFKAFLDYALMLGADLIA 129 Query: 123 TGHYARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRRIAEQ 182 TGHY R R+ G +LLK LD +KDQSYFLH + EQ++R++FP+GE+ K EVR IAE+ Sbjct: 130 TGHYVR-RRDRDGRTELLKGLDPNKDQSYFLHAVGGEQIARSLFPVGELEKPEVRAIAEK 188 Query: 183 LQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQRK 242 L AKKKDSTGICFIGER F +FL +YL +PG I+T +G+ +G H GL ++T+GQR+ Sbjct: 189 HGLATAKKKDSTGICFIGERRFTDFLKQYLPAQPGDIETTEGKVIGRHSGLMYHTIGQRQ 248 Query: 243 GIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQVSWVAG-H 301 G+GIGGLK + D WYV KD++ N L + QG+DHP L S L A ++ WV Sbjct: 249 GLGIGGLK-----EAGDDPWYVLGKDLQRNVLLVGQGNDHPLLFSRALLASRIYWVNPVE 303 Query: 302 APQMAALSAKTRYRQADVPCGFSDAGASFSLAFDTPQWAVTPGQSAVLYDGDVCLGGGII 361 + L AK RYRQ+D C + FD PQ AVTPGQS V YDGDVCLGGG+I Sbjct: 304 LERPRRLRAKVRYRQSDQDCVLEKTAEGYRAVFDEPQRAVTPGQSVVFYDGDVCLGGGVI 363 Query: 362 ET 363 ET Sbjct: 364 ET 365 >STM1234 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridylate) methyltransferase, contains the PP-loop ATPase domain Sty Salmonella typhimurium LT2 Length = 383 Score = 421 bits (1081), Expect = e-117, Method: Compositional matrix adjust. Identities = 217/365 (59%), Positives = 263/365 (72%), Gaps = 11/365 (3%) Query: 2 SKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVAD 61 S K+V++GMSGGVDSSVSAWLL++QGY+V GLFMKNWE+DD EYC+ D DA +V D Sbjct: 19 SPKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDDGEEYCTAAADLADAQAVCD 78 Query: 62 VVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMK-LGADL 120 +G+++ VNFA+EY D VF FL EY+AGRTPNPD+LCN EIKFKAFL+ A + LGAD Sbjct: 79 KLGIELHTVNFAAEYWDNVFELFLEEYKAGRTPNPDILCNKEIKFKAFLEFAAEDLGADY 138 Query: 121 IATGHYARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRRIA 180 IATGHY R R G +LL+ LD +KDQSYFL+ L EQ+++++FP+GE+ K +VR+IA Sbjct: 139 IATGHYVR-RADVNGKSRLLRGLDGNKDQSYFLYTLGHEQIAQSLFPVGELEKPQVRKIA 197 Query: 181 EQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQ 240 E L L AKKKDSTGICFIGER FR+FL RYL +PG I T DG +GEH GL ++TLGQ Sbjct: 198 EDLGLVTAKKKDSTGICFIGERKFRDFLGRYLPAQPGKIITVDGDEIGEHQGLMYHTLGQ 257 Query: 241 RKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQVSWVAG 300 RKG+GIGG K DG D WYV KDVENN L + QGH+HP L+S L A Q+ WV Sbjct: 258 RKGLGIGGTK-----DGSEDPWYVVDKDVENNVLIVAQGHEHPRLMSVGLIAQQLHWV-D 311 Query: 301 HAPQMAAL--SAKTRYRQADVPCGFSDAGAS-FSLAFDTPQWAVTPGQSAVLYDGDVCLG 357 P L + KTRYRQ D+PC + + FD P AVTPGQSAV Y G+VCLG Sbjct: 312 REPFTGTLRCTVKTRYRQTDIPCTINALNDDRIEVIFDEPVAAVTPGQSAVFYSGEVCLG 371 Query: 358 GGIIE 362 GGIIE Sbjct: 372 GGIIE 376 >ZycfB [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridylate) methyltransferase, contains the PP-loop ATPase domain EcZ Escherichia coli O157:H7 EDL933 Length = 383 Score = 416 bits (1070), Expect = e-116, Method: Compositional matrix adjust. Identities = 214/365 (58%), Positives = 265/365 (72%), Gaps = 11/365 (3%) Query: 2 SKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVAD 61 + K+V++GMSGGVDSSVSAWLL++QGY+V GLFMKNWE+DD EYC+ D DA +V D Sbjct: 19 TAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDDGEEYCTAAADLADAQAVCD 78 Query: 62 VVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMK-LGADL 120 +G+++ VNFA+EY D VF FL EY+AGRTPNPD+LCN EIKFKAFL+ A + LGAD Sbjct: 79 KLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILCNKEIKFKAFLEFAAEDLGADY 138 Query: 121 IATGHYARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRRIA 180 IATGHY R R G +LL+ LD++KDQSYFL+ L+ EQ+++++FP+GE+ K +VR+IA Sbjct: 139 IATGHYVR-RADVDGKSRLLRGLDSNKDQSYFLYTLSHEQIAQSLFPVGELEKPQVRKIA 197 Query: 181 EQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQ 240 E L L AKKKDSTGICFIGER FREFL RYL +PG I T DG +GEH GL ++TLGQ Sbjct: 198 EDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQPGKIITVDGDEIGEHQGLMYHTLGQ 257 Query: 241 RKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQVSWVAG 300 RKG+GIGG K +G + WYV KDVENN L + QGH+HP L+S L A Q+ WV Sbjct: 258 RKGLGIGGTK-----EGTEEPWYVVDKDVENNILIVAQGHEHPRLMSVGLIAQQLHWV-D 311 Query: 301 HAPQMAAL--SAKTRYRQADVPCGFSDAGAS-FSLAFDTPQWAVTPGQSAVLYDGDVCLG 357 P + + KTRYRQ D+PC + FD P AVTPGQSAV Y+G+VCLG Sbjct: 312 REPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFDEPVAAVTPGQSAVFYNGEVCLG 371 Query: 358 GGIIE 362 GGIIE Sbjct: 372 GGIIE 376 >ECs1605 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridylate) methyltransferase, contains the PP-loop ATPase domain Ecs Escherichia coli O157:H7 Length = 383 Score = 416 bits (1070), Expect = e-116, Method: Compositional matrix adjust. Identities = 214/365 (58%), Positives = 265/365 (72%), Gaps = 11/365 (3%) Query: 2 SKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVAD 61 + K+V++GMSGGVDSSVSAWLL++QGY+V GLFMKNWE+DD EYC+ D DA +V D Sbjct: 19 TAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDDGEEYCTAAADLADAQAVCD 78 Query: 62 VVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMK-LGADL 120 +G+++ VNFA+EY D VF FL EY+AGRTPNPD+LCN EIKFKAFL+ A + LGAD Sbjct: 79 KLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILCNKEIKFKAFLEFAAEDLGADY 138 Query: 121 IATGHYARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRRIA 180 IATGHY R R G +LL+ LD++KDQSYFL+ L+ EQ+++++FP+GE+ K +VR+IA Sbjct: 139 IATGHYVR-RADVDGKSRLLRGLDSNKDQSYFLYTLSHEQIAQSLFPVGELEKPQVRKIA 197 Query: 181 EQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQ 240 E L L AKKKDSTGICFIGER FREFL RYL +PG I T DG +GEH GL ++TLGQ Sbjct: 198 EDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQPGKIITVDGDEIGEHQGLMYHTLGQ 257 Query: 241 RKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQVSWVAG 300 RKG+GIGG K +G + WYV KDVENN L + QGH+HP L+S L A Q+ WV Sbjct: 258 RKGLGIGGTK-----EGTEEPWYVVDKDVENNILIVAQGHEHPRLMSVGLIAQQLHWV-D 311 Query: 301 HAPQMAAL--SAKTRYRQADVPCGFSDAGAS-FSLAFDTPQWAVTPGQSAVLYDGDVCLG 357 P + + KTRYRQ D+PC + FD P AVTPGQSAV Y+G+VCLG Sbjct: 312 REPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFDEPVAAVTPGQSAVFYNGEVCLG 371 Query: 358 GGIIE 362 GGIIE Sbjct: 372 GGIIE 376 >trmU [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridylate) methyltransferase, contains the PP-loop ATPase domain Eco Escherichia coli K12 Length = 383 Score = 416 bits (1070), Expect = e-116, Method: Compositional matrix adjust. Identities = 214/365 (58%), Positives = 265/365 (72%), Gaps = 11/365 (3%) Query: 2 SKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVAD 61 + K+V++GMSGGVDSSVSAWLL++QGY+V GLFMKNWE+DD EYC+ D DA +V D Sbjct: 19 TAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDDGEEYCTAAADLADAQAVCD 78 Query: 62 VVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMK-LGADL 120 +G+++ VNFA+EY D VF FL EY+AGRTPNPD+LCN EIKFKAFL+ A + LGAD Sbjct: 79 KLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILCNKEIKFKAFLEFAAEDLGADY 138 Query: 121 IATGHYARVRQGPQGDFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRRIA 180 IATGHY R R G +LL+ LD++KDQSYFL+ L+ EQ+++++FP+GE+ K +VR+IA Sbjct: 139 IATGHYVR-RADVDGKSRLLRGLDSNKDQSYFLYTLSHEQIAQSLFPVGELEKPQVRKIA 197 Query: 181 EQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTLGQ 240 E L L AKKKDSTGICFIGER FREFL RYL +PG I T DG +GEH GL ++TLGQ Sbjct: 198 EDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQPGKIITVDGDEIGEHQGLMYHTLGQ 257 Query: 241 RKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQVSWVAG 300 RKG+GIGG K +G + WYV KDVENN L + QGH+HP L+S L A Q+ WV Sbjct: 258 RKGLGIGGTK-----EGTEEPWYVVDKDVENNILVVAQGHEHPRLMSVGLIAQQLHWV-D 311 Query: 301 HAPQMAAL--SAKTRYRQADVPCGFSDAGAS-FSLAFDTPQWAVTPGQSAVLYDGDVCLG 357 P + + KTRYRQ D+PC + FD P AVTPGQSAV Y+G+VCLG Sbjct: 312 REPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFDEPVAAVTPGQSAVFYNGEVCLG 371 Query: 358 GGIIE 362 GGIIE Sbjct: 372 GGIIE 376 >VC1128 [J] COG0482 Predicted tRNA(5-methylaminomethyl-2-thiouridylate) methyltransferase, contains the PP-loop ATPase domain Vch Vibrio cholerae Length = 372 Score = 411 bits (1057), Expect = e-114, Method: Compositional matrix adjust. Identities = 211/367 (57%), Positives = 264/367 (71%), Gaps = 13/367 (3%) Query: 2 SKKRVVIGMSGGVDSSVSAWLLKEQGYEVIGLFMKNWEDDDDSEYCSTREDWIDAVSVAD 61 S+K+V++GMSGGVDSSVSA+LLK+QGY+V GLFMKNWE+DD+ EYC+ ED DA +V D Sbjct: 10 SQKKVIVGMSGGVDSSVSAYLLKQQGYQVEGLFMKNWEEDDNEEYCTAAEDLADAQAVCD 69 Query: 62 VVGVDIEAVNFASEYKDRVFAEFLREYQAGRTPNPDVLCNAEIKFKAFLDHAMK-LGADL 120 +G+ + +NFA+EY D VF FL EY+AGRTPNPD+LCN EIKFKAFL+ A + L AD Sbjct: 70 KLGIPLHTINFAAEYWDNVFEYFLAEYKAGRTPNPDILCNKEIKFKAFLEFADEVLDADY 129 Query: 121 IATGHYARVRQGPQG--DFQLLKALDASKDQSYFLHRLNQEQLSRTVFPLGEIHKTEVRR 178 IA GHY R R PQ Q+L+ LD +KDQSYFL+ L+ EQ++RT+FP+GE+ K EVRR Sbjct: 130 IAMGHYVR-RTFPQNGEKPQMLRGLDGNKDQSYFLYTLSHEQVARTLFPVGELEKPEVRR 188 Query: 179 IAEQLQLPNAKKKDSTGICFIGERPFREFLNRYLSYKPGPIKTADGQTVGEHVGLSFYTL 238 IAE+ L AKKKDSTGICFIGER F +FL+RYL +PG I+T +G+ +GEH GL ++TL Sbjct: 189 IAEEQGLITAKKKDSTGICFIGERKFTDFLSRYLPAQPGKIETPEGKVIGEHQGLMYHTL 248 Query: 239 GQRKGIGIGGLKAHQNADGHGDAWYVARKDVENNTLYIVQGHDHPWLLSPTLSAGQVSWV 298 GQRKG+ IGG+K + WYVA KD++ N L VQG DHP L S L A Q+ WV Sbjct: 249 GQRKGLHIGGMK-----ESSEQPWYVADKDLKRNVLIAVQGADHPLLKSHGLVAAQLHWV 303 Query: 299 AGHAP--QMAALSAKTRYRQADVPCGFSD-AGASFSLAFDTPQWAVTPGQSAVLYDGDVC 355 P + S KTRYRQ+D+ C + + FD PQ AVTPGQSAV Y G++C Sbjct: 304 -DRTPITEPVRCSVKTRYRQSDIACTIIPLSDDRIKVMFDEPQVAVTPGQSAVFYQGEIC 362 Query: 356 LGGGIIE 362 LGGGIIE Sbjct: 363 LGGGIIE 369