BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0156.p (408 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AGc24 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Atu Ag... 402 e-112 SMc02770 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Sme... 390 e-108 mll4552 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Mlo ... 376 e-104 CC0250 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Ccr C... 365 e-100 RSc1008 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Rso ... 348 1e-95 RP681 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Rpr Ri... 329 8e-90 RC1043 Non COG 191 4e-48 >AGc24 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 418 Score = 402 bits (1034), Expect = e-112, Method: Compositional matrix adjust. Identities = 200/406 (49%), Positives = 262/406 (64%), Gaps = 3/406 (0%) Query: 5 YQLYQNYADATDPLRACARMMAQALGATWPGIPVHPYWRKMASACEVFARTQLTHARPPF 64 Y LY+ A PLRA A MM QA + + R + + EVF R + +P F Sbjct: 11 YHLYEMNHAAMAPLRAGADMMRQACNNPLNPLSSTAFGRSLDAGFEVFERLTRRYVKPEF 70 Query: 65 GIATVEEEGRTISVHEEEIHATPFCGLLHFRKN---SATVQPKVLVVAPMSGHFATLLRG 121 + + +G+T+ V +E + + PFC L+HF ++ + PKVL+VAPMSGH+ATLLRG Sbjct: 71 DLGSTSVDGQTVVVTDETVWSRPFCSLVHFNRDLEPTRKPDPKVLLVAPMSGHYATLLRG 130 Query: 122 TVRTLLRDHDVYITDWRNARDVATAHGRFGLDEYVSHIIDFLGVLGPGAHLLAVCQPTVA 181 TV LL D+YITDW +AR V G F LD+Y+ ++I+ L +GPGAH++AVCQP+V Sbjct: 131 TVEALLPSADIYITDWADARIVPATEGTFDLDDYIDYVIEMLRHIGPGAHVVAVCQPSVP 190 Query: 182 ALTAAAVMAADGHPAQPRSMTLMAGPIDTRVNPTAVNALAKSKPIAWFEKNMISTVPARH 241 L A ++M ADG P SMTLM GPIDTR+NPTAVN LA +KPI WF N++ VP Sbjct: 191 VLAAVSLMEADGDTFAPASMTLMGGPIDTRINPTAVNGLATAKPIEWFRDNVVMQVPWPQ 250 Query: 242 AGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKLYHHLVDGEHARADQIKDFYEEYFAMADL 301 GR VYPGF+QL+ FM+MNL +H+ A Y +LV + A++ ++FY+EY A+ DL Sbjct: 251 PAFGRYVYPGFLQLSGFMSMNLDKHMTAHKDFYLNLVKNDGDSAEKHREFYDEYLAVMDL 310 Query: 302 PAEFYLETVRTVFQEHALPLGKLSYAGRPVEPRAIRRTALFTIEGEKDDICAVGQTLAAQ 361 AEFYL+TV TVF +HALP G + + GR V+P AI ALFT+EGE DDI VGQT AA Sbjct: 311 TAEFYLQTVETVFIDHALPKGNMLHRGRAVDPTAIHNVALFTVEGENDDISGVGQTKAAH 370 Query: 362 ELCSGIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMHH 407 +LC I R H++Q VGHYGVFNG R+ EI PR+ DFI H Sbjct: 371 DLCRNIPEDKRAHYMQPDVGHYGVFNGSRFRKEIVPRMLDFIGKHQ 416 >SMc02770 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Sme Sinorhizobium meliloti Length = 424 Score = 390 bits (1003), Expect = e-108, Method: Compositional matrix adjust. Identities = 203/409 (49%), Positives = 260/409 (63%), Gaps = 7/409 (1%) Query: 5 YQLYQNYADATDPLRACARMMAQALGATWPGIPV-HPYW-RKMASACEVFARTQLTHARP 62 YQLY+ P R A M A P PV H Y+ R A+ EVF R + +P Sbjct: 3 YQLYEMNHAMMAPWRTAADAMRLAFSN--PMNPVSHTYFGRAAAAGLEVFERATRRYGKP 60 Query: 63 PFGIATVEEEGRTISVHEEEIHATPFCGLLHFRKN---SATVQPKVLVVAPMSGHFATLL 119 FG+ E +G+ + V E + PFC L+HF ++ PKVL+VAPMSGH+ATLL Sbjct: 61 EFGLPETEIDGQPVPVRERIVWREPFCNLIHFERSLPRDRAPDPKVLIVAPMSGHYATLL 120 Query: 120 RGTVRTLLRDHDVYITDWRNARDVATAHGRFGLDEYVSHIIDFLGVLGPGAHLLAVCQPT 179 RGTV LL DVYITDW +AR V + G F LD+Y+ ++I L LGP AH++ VCQP Sbjct: 121 RGTVEALLPHSDVYITDWIDARMVPLSEGTFDLDDYIDYVIQMLHFLGPDAHVIGVCQPA 180 Query: 180 VAALTAAAVMAADGHPAQPRSMTLMAGPIDTRVNPTAVNALAKSKPIAWFEKNMISTVPA 239 V L A A+M A P P +MTLM GPIDTR+NPTAVN LAK +PI WF N++ VP Sbjct: 181 VPVLAAVALMEAAEDPLAPSTMTLMGGPIDTRINPTAVNELAKERPIEWFRDNVVMPVPW 240 Query: 240 RHAGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKLYHHLVDGEHARADQIKDFYEEYFAMA 299 G R VYPGF+QL+ FM+MNL RH+ A + + HLV + A++ +DFY+EY A+ Sbjct: 241 PQPGFMRMVYPGFLQLSGFMSMNLDRHLIAHKEFFAHLVKNDGDAAEKHRDFYDEYLAVM 300 Query: 300 DLPAEFYLETVRTVFQEHALPLGKLSYAGRPVEPRAIRRTALFTIEGEKDDICAVGQTLA 359 DL AEFYL+TV+ VF +HALP G++ + G+ V+P AIRR AL T+EGE DDI +GQT A Sbjct: 301 DLTAEFYLQTVQVVFMQHALPKGEMIHRGKRVDPTAIRRVALLTVEGENDDISGLGQTKA 360 Query: 360 AQELCSGIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMHHR 408 AQ +C+GI MR H++Q VGHYGVFNG R+ EI PR+ F H R Sbjct: 361 AQTICTGIPDSMRQHYMQPDVGHYGVFNGSRFRREIAPRIVAFQRRHSR 409 >mll4552 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Mlo Mesorhizobium loti Length = 425 Score = 376 bits (965), Expect = e-104, Method: Compositional matrix adjust. Identities = 196/411 (47%), Positives = 262/411 (63%), Gaps = 11/411 (2%) Query: 5 YQLYQNYADATDPLRACARMMAQALGATW--PGIPV-HPYW-RKMASACEVFARTQLTHA 60 YQLY+ A P AR+ A A+ + P P+ H +W R +A+ E+F RT + Sbjct: 3 YQLYELNHAALQP----ARLYADAVRMFYSNPLNPIAHTHWGRSVAAGAELFERTTRRYG 58 Query: 61 RPPFGIATVEEEGRTISVHEEEIHATPFCGLLHFRKN---SATVQPKVLVVAPMSGHFAT 117 +P FG+ + +++ V E+ + + PFC L+ F + PK+L+VAPMSGH+AT Sbjct: 59 KPAFGLDKTVVDWKSVEVTEKTVWSKPFCNLVRFERAMPAGRKPDPKLLIVAPMSGHYAT 118 Query: 118 LLRGTVRTLLRDHDVYITDWRNARDVATAHGRFGLDEYVSHIIDFLGVLGPGAHLLAVCQ 177 LLRGTV +L DV+ITDW +AR V G F LD+Y+ +II+ LGP H++AVCQ Sbjct: 119 LLRGTVEAMLPYADVHITDWVDARMVPLVDGSFDLDDYIDYIIEMFHALGPDTHVMAVCQ 178 Query: 178 PTVAALTAAAVMAADGHPAQPRSMTLMAGPIDTRVNPTAVNALAKSKPIAWFEKNMISTV 237 P+V L A A+M A G P P +MTLM GPIDTR NPTAVN LA+ K I WF N++ Sbjct: 179 PSVPVLAAVALMEAKGDPFVPSTMTLMGGPIDTRRNPTAVNLLAEEKGIDWFRDNVVMRA 238 Query: 238 PARHAGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKLYHHLVDGEHARADQIKDFYEEYFA 297 P G GR VYPGF+QL+ FM+MNL RH+ A + HLV + A++ +DFY+EY A Sbjct: 239 PWPVPGFGREVYPGFLQLSGFMSMNLDRHIIAHKDFFMHLVKNDGDNAEKHRDFYDEYMA 298 Query: 298 MADLPAEFYLETVRTVFQEHALPLGKLSYAGRPVEPRAIRRTALFTIEGEKDDICAVGQT 357 + DL AEFYL+TV TVF HALP G++ + G ++ AIR ALFT+EGE DDI +GQT Sbjct: 299 VMDLTAEFYLQTVDTVFVRHALPKGEMKHRGAAIDTSAIRNVALFTVEGENDDISGLGQT 358 Query: 358 LAAQELCSGIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMHHR 408 AAQ+LC I + H++Q AVGHYGVFNG R+ +EI PR+ DFI + R Sbjct: 359 KAAQDLCINIPADKKAHYMQPAVGHYGVFNGSRFRSEIVPRIVDFITSYGR 409 >CC0250 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Ccr Caulobacter vibrioides Length = 427 Score = 365 bits (936), Expect = e-100, Method: Compositional matrix adjust. Identities = 188/412 (45%), Positives = 258/412 (62%), Gaps = 18/412 (4%) Query: 5 YQLYQNYADATDPLRACARMMAQALGATWPGIPVHP-----YWRKMASACEVFARTQLTH 59 Y L++ A+ P+R + A A W G P++P R+M + ++FA + Sbjct: 10 YALHEAAYYASTPMR----LAALAARDFW-GSPLNPAADSAVGRRMHAGADLFANVTRRY 64 Query: 60 ARPPFGIATVEEEGRTISVHEEEIHATPFCGLLHFRKNSA--------TVQPKVLVVAPM 111 +P + I V+ + V + +P+ L+ F ++ A ++ P VL+VAP+ Sbjct: 65 GKPKWNIDKVKVGQVDVRVRPTVVWESPWARLVQFDRDMADMRRAGKFSLDPAVLIVAPL 124 Query: 112 SGHFATLLRGTVRTLLRDHDVYITDWRNARDVATAHGRFGLDEYVSHIIDFLGVLGPGAH 171 SGH+ATLLRGTV L DH V+I DW NAR+V+ GRF +Y+ H+I L VLGP + Sbjct: 125 SGHYATLLRGTVEAFLPDHAVFIVDWVNAREVSVLEGRFDFHDYIDHVIQMLEVLGPRPN 184 Query: 172 LLAVCQPTVAALTAAAVMAADGHPAQPRSMTLMAGPIDTRVNPTAVNALAKSKPIAWFEK 231 ++AVCQP L AAA+MA HP++P SMT M PID R++PT N LA+ KP WF+ Sbjct: 185 VVAVCQPGPPVLAAAALMAEQNHPSRPASMTFMGSPIDARLSPTVTNQLAEEKPFTWFQS 244 Query: 232 NMISTVPARHAGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKLYHHLVDGEHARADQIKDF 291 NMI VP +AGAGRRVYPGFVQLA+FM+MN RH EA + ++HLV G+ AD+ +F Sbjct: 245 NMIYNVPPPYAGAGRRVYPGFVQLASFMSMNADRHQEAHRRYFNHLVSGDGDSADKHLEF 304 Query: 292 YEEYFAMADLPAEFYLETVRTVFQEHALPLGKLSYAGRPVEPRAIRRTALFTIEGEKDDI 351 Y+EY ++ DL EFYL+T+ VFQ++ LP G+L + G V+P I L T+EGE DDI Sbjct: 305 YDEYLSVLDLTEEFYLQTIDIVFQQYLLPKGELVHRGTRVKPEVITDIGLMTVEGENDDI 364 Query: 352 CAVGQTLAAQELCSGIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFI 403 +GQT AA LC+ I M+L +VQ +VGHYGVFNGRR+ EIYPR+R FI Sbjct: 365 SGIGQTQAAHGLCANIPEDMKLDYVQPSVGHYGVFNGRRFREEIYPRVRKFI 416 >RSc1008 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Rso Ralstonia solanacearum Length = 422 Score = 348 bits (894), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 185/408 (45%), Positives = 255/408 (62%), Gaps = 10/408 (2%) Query: 5 YQLYQNYADATDPLRACARMMAQALGATWPGIPVHPYWRKMASACEVFARTQLTHARPPF 64 YQL++ P+ A A+ A+ + + P ++ A+ E+ R + +P F Sbjct: 3 YQLHEFQRAMLSPMTAWAQATAKTFTNPLSPLSLLPGAQRYAAGYELLYRLGKEYEKPEF 62 Query: 65 GIATVEEEGRTISVHEEEIHATPFCGLLHFRK---NSATV-----QPKVLVVAPMSGHFA 116 I +V GR I + E+ I PFC L+ F++ ++ T+ +P VLV AP+SGH + Sbjct: 63 NIRSVRSNGRDIPIVEQTIIEKPFCRLVRFKRYADDAETIRMLKDEPVVLVCAPLSGHHS 122 Query: 117 TLLRGTVRTLLRDHDVYITDWRNARDVATAHGRFGLDEYVSHIIDFLGVLGPGA-HLLAV 175 TLLR TVRTLL+DH VY+TDW +AR V G F L +Y+++I +F+ +G H+++V Sbjct: 123 TLLRDTVRTLLQDHKVYVTDWLDARMVPVGAGPFHLSDYIAYIREFIAHIGADKLHVISV 182 Query: 176 CQPTVAALTAAAVMAADGHPAQPRSMTLMAGPIDTRVNPTAVNALAKSKPIAWFEKNMIS 235 CQPTV L A ++MA+DG P +MT+M GPID R +PTAVN+LA +K AWFE N+I Sbjct: 183 CQPTVPVLAAISLMASDGEKT-PLTMTMMGGPIDARKSPTAVNSLATNKSHAWFENNVIY 241 Query: 236 TVPARHAGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKLYHHLVDGEHARADQIKDFYEEY 295 TVPA + G GRRVYPGF+Q A F+ MN RHV + Y LV+G+ A+ FY+EY Sbjct: 242 TVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVEGDKEDAEAHVRFYDEY 301 Query: 296 FAMADLPAEFYLETVRTVFQEHALPLGKLSYAGRPVEPRAIRRTALFTIEGEKDDICAVG 355 A+ D+ AE+YL+T+R VFQE L G G+PV P+ I+ TALFTIEGE DDI G Sbjct: 302 NAVLDMAAEYYLDTIRDVFQEFHLANGTWVVDGKPVRPQDIKGTALFTIEGELDDISGSG 361 Query: 356 QTLAAQELCSGIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFI 403 QT AA +LCS I + H + GHYG+F+GRRW +YP+LRDFI Sbjct: 362 QTEAAHDLCSSIPKTRKQHLTASNCGHYGIFSGRRWREFVYPQLRDFI 409 >RP681 [I] COG4553 Poly-beta-hydroxyalkanoate depolymerase Rpr Rickettsia prowazekii Length = 423 Score = 329 bits (843), Expect = 8e-90, Method: Compositional matrix adjust. Identities = 157/358 (43%), Positives = 225/358 (62%), Gaps = 4/358 (1%) Query: 47 SACEVFARTQLTHARPPFGIATVEEEGRTISVHEEEIHATPFCGLLHFRKNSATVQ-PKV 105 + CE R + +P F I +T +++E+ I PFC L HF+K + PK+ Sbjct: 55 TLCERMTRK---YTKPEFNILETIINDKTYNINEQVIFKKPFCELRHFQKIGFKKELPKL 111 Query: 106 LVVAPMSGHFATLLRGTVRTLLRDHDVYITDWRNARDVATAHGRFGLDEYVSHIIDFLGV 165 L+VAPM+GH ATLLR TV LL D+YITDW A V G F +D+Y+ ++I+F+ Sbjct: 112 LIVAPMAGHHATLLRSTVHALLPYTDIYITDWTEANYVPLEAGHFDMDDYIDYLIEFINF 171 Query: 166 LGPGAHLLAVCQPTVAALTAAAVMAADGHPAQPRSMTLMAGPIDTRVNPTAVNALAKSKP 225 +G H +AVCQPTV L A ++M+ + P P SM L+ GPID R NPT VN A SK Sbjct: 172 MGQNIHTMAVCQPTVPLLAAISLMSENNSPNVPSSMILIGGPIDARKNPTVVNEFALSKS 231 Query: 226 IAWFEKNMISTVPARHAGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKLYHHLVDGEHARA 285 + WF + + VP + G GR+VYPGF+QL F+++NL RH+++ +L+ L++ ++ +A Sbjct: 232 LEWFCEMLTMQVPPNYPGYGRKVYPGFLQLTGFISLNLLRHIDSHLELWQSLLNSDYQKA 291 Query: 286 DQIKDFYEEYFAMADLPAEFYLETVRTVFQEHALPLGKLSYAGRPVEPRAIRRTALFTIE 345 D I FY+EY + D+PAEFYL+T+ VFQ+ +L GK RP++ + I + AL IE Sbjct: 292 DYIIKFYDEYLSGMDMPAEFYLQTIDEVFQQFSLARGKFISEKRPIDLKHITKCALLGIE 351 Query: 346 GEKDDICAVGQTLAAQELCSGIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFI 403 GE DDI AVGQT AA +LC I M+ +H+Q VGHYGVF+G ++ I P +++FI Sbjct: 352 GELDDIAAVGQTKAALKLCVNISESMKRYHLQNGVGHYGVFSGSKFKQFIVPLIKEFI 409 >RC1043 Non COG Length = 197 Score = 191 bits (484), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 92/204 (45%), Positives = 128/204 (62%), Gaps = 9/204 (4%) Query: 111 MSGHFATLLRGTVRTLLRDHDVYITDWRNARDVATAHGRFGLDEYVSHIIDFLGVLGPGA 170 M+GH ATLLR TV+ LL +YITDW A V G F +D+Y+ ++++ + +GP Sbjct: 3 MAGHHATLLRTTVQELLLYTGIYITDWTEASYVPLEAGHFDMDDYIDYVMECINFIGPNV 62 Query: 171 HLLAVCQPTVAALTAAAVMAADGHPAQPRSMTLMAGPIDTRVNPTAVNALAKSKPIAWFE 230 H +AVCQPTV L A ++M+ P P SM LM GPID R NPTAVN A+SK + WF Sbjct: 63 HTMAVCQPTVPLLAAISLMSESNSPNVPSSMILMGGPIDARKNPTAVNEFAQSKSLEWFC 122 Query: 231 KNMISTVPARHAGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKLYHHLVDGEHARADQIKD 290 + M+ VP YPGF+QLA FM +NL RH+++ +L+ L++ ++ +AD Sbjct: 123 Q-MVMQVPPN--------YPGFLQLAVFMGLNLCRHIDSHLELWQSLLNSDYKKADHTIK 173 Query: 291 FYEEYFAMADLPAEFYLETVRTVF 314 FY EY A ++PAEFYL+T+ VF Sbjct: 174 FYYEYLAGMNMPAEFYLQTIDEVF 197