BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0100.p (241 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA4098 [IQR] COG1028 Dehydrogenases with different specificities... 205 7e-53 SMc02336 [IQR] COG1028 Dehydrogenases with different specificiti... 196 3e-50 BS_kduD [IQR] COG1028 Dehydrogenases with different specificitie... 181 1e-45 CAC0361 [IQR] COG1028 Dehydrogenases with different specificitie... 167 1e-41 SMb21348 [IQR] COG1028 Dehydrogenases with different specificiti... 162 7e-40 BH2167 [IQR] COG1028 Dehydrogenases with different specificities... 161 1e-39 AGl3337 [IQR] COG1028 Dehydrogenases with different specificitie... 160 2e-39 TM0441 [IQR] COG1028 Dehydrogenases with different specificities... 157 2e-38 mll4054 [IQR] COG1028 Dehydrogenases with different specificitie... 154 1e-37 aq_1716 [IQR] COG1028 Dehydrogenases with different specificitie... 154 2e-37 >PA4098 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Pae Pseudomonas aeruginosa Length = 241 Score = 205 bits (522), Expect = 7e-53, Method: Compositional matrix adjust. Identities = 112/241 (46%), Positives = 145/241 (60%) Query: 1 MSFIPDLLKGKTALVTGGTQGIGAAVARQLAALGARVIAAGLPPFAHEEFAGSAVTLVEM 60 M F D+ G+ LVTGG+ GIGAA+A Q A LGA V+A GL + E+ Sbjct: 1 MVFQHDIYAGQQVLVTGGSSGIGAAIAMQFAELGAEVVALGLDADGVHAPRHPRIRREEL 60 Query: 61 NVASEESVRAALAGLTQLDIVVNCAGIIKRGAELETEVFEQVIAVNLTGTMRVCAAAREL 120 ++ + ++ L +LD++VN AGI + E + FE+V+ +NL+ M AR L Sbjct: 61 DITDSQRLQRLFEALPRLDVLVNNAGISRDREEYDLATFERVLRLNLSAAMLASQLARPL 120 Query: 121 LRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGWIA 180 L Q G I+N ASM S FG P YSASKG + QLT+SLA YAA+ IRVNA+APGWI Sbjct: 121 LAQRGGSILNIASMYSTFGSADRPAYSASKGAIVQLTRSLACEYAAERIRVNAIAPGWID 180 Query: 181 TPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGGYLV 240 TPL L+ D I+ RTPL RWG+ +V AFLC P ASF+TGA++ VDGGYL Sbjct: 181 TPLGAGLKADVEATRRIMQRTPLARWGEAPEVASAAAFLCGPGASFVTGAVLAVDGGYLC 240 Query: 241 A 241 A Sbjct: 241 A 241 >SMc02336 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 252 Score = 196 bits (499), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 112/243 (46%), Positives = 147/243 (60%), Gaps = 4/243 (1%) Query: 2 SFIPDLLKGKTALVTGGTQGIGAAVARQLAALGARVIAAGLPPF----AHEEFAGSAVTL 57 +F PDL GK LVTGG+ GIG A+A+ A LG VIA G A + +A+ Sbjct: 7 TFRPDLFAGKDILVTGGSSGIGLAIAQGFARLGGSVIALGSSETKLKAAAADPENTAIRF 66 Query: 58 VEMNVASEESVRAALAGLTQLDIVVNCAGIIKRGAELETEVFEQVIAVNLTGTMRVCAAA 117 ++V E++ A L +LD++VN AGI + AE E + +V+ VNL MR A Sbjct: 67 ERVDVRDPEAINAFARTLRKLDVLVNGAGIARPEAEFEDATYMEVMDVNLNSQMRFAMAV 126 Query: 118 RELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPG 177 LL+ SRG I+NTASMLS+ LVP Y ASK GV LT+ LA A+ DGIRVNA++PG Sbjct: 127 LPLLKASRGVILNTASMLSYVSDPLVPAYGASKSGVLGLTRHLAHAFGPDGIRVNAISPG 186 Query: 178 WIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDGG 237 + T +T+ L DPV A I R+ L RWG +D+ FL SPAA F+TGA +PVDGG Sbjct: 187 YHKTDMTKGLWTDPVPAEKIAQRSALKRWGTVDDLVGTALFLASPAAKFITGADLPVDGG 246 Query: 238 YLV 240 ++V Sbjct: 247 FVV 249 >BS_kduD [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bsu Bacillus subtilis Length = 254 Score = 181 bits (459), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 108/252 (42%), Positives = 149/252 (59%), Gaps = 13/252 (5%) Query: 1 MSFIPDL--LKGKTALVTGGTQGIGAAVARQLAALGARVIAAGLPPFAHE-----EFAGS 53 M ++ D LKGKTALVTG GIG +A+ LA GA +I E E G Sbjct: 1 MGYLHDAFSLKGKTALVTGPGTGIGQGIAKALAGAGADIIGTSHTSSLSETQQLVEQEGR 60 Query: 54 AVTLVEMNVASEESVRAALAGL---TQLDIVVNCAGIIKR--GAELETEVFEQVIAVNLT 108 T ++++ E+++ + A L Q+DI+VN AGII R + E ++ V+ VNL Sbjct: 61 IFTSFTLDMSKPEAIKDSAAELFENRQIDILVNNAGIIHREKAEDFPEENWQHVLNVNLN 120 Query: 109 GTMRVCA-AARELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAAD 167 + A R +L++ G I+N AS+LSF GG LVP Y+ASK VA LTKS A +AA Sbjct: 121 SLFILTQLAGRHMLKRGHGKIINIASLLSFQGGILVPAYTASKHAVAGLTKSFANEWAAS 180 Query: 168 GIRVNAVAPGWIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFM 227 GI+VNA+APG+I+T T+ ++DD R IL R P GRWGQ +D+G FL S A+ ++ Sbjct: 181 GIQVNAIAPGYISTANTKPIRDDEKRNEDILKRIPAGRWGQADDIGGTAVFLASRASDYV 240 Query: 228 TGAIVPVDGGYL 239 G I+ VDGG+L Sbjct: 241 NGHILAVDGGWL 252 >CAC0361 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Cac Clostridium acetobutylicum Length = 260 Score = 167 bits (424), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 102/252 (40%), Positives = 140/252 (55%), Gaps = 14/252 (5%) Query: 1 MSFIPDLLKGKTALVTGGTQGIGAAVARQLAALGARVIAAGLPPFAHE-----EFAGSAV 55 M F LKGK A+VTGG G+G A LA GA + E E G V Sbjct: 10 MDFFS--LKGKVAIVTGGNTGLGQGYALALAKAGADLFIVTHGTNWDETRKLIEDEGRKV 67 Query: 56 TLVEMNVASEESV----RAALAGLTQLDIVVNCAGIIKRG--AELETEVFEQVIAVNLTG 109 + +++ +ES+ + +LDI+VN AG I+R E E +E +I NL Sbjct: 68 EFYKADLSKKESIPGIFDKCMELYGRLDILVNNAGTIRRAPITEYRDEDWEYIINTNLNS 127 Query: 110 TMRVCA-AARELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADG 168 + AA+ +++Q G I+N ASMLSF GG VP Y+ASK GVA LTK+ A A Sbjct: 128 IYHLSQRAAKIMIKQGSGKIINIASMLSFQGGKFVPPYTASKHGVAGLTKAFANELADKN 187 Query: 169 IRVNAVAPGWIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMT 228 I++NA+APG+I T T+A+++D R IL+R P G+WG P DV V FL S A+ ++ Sbjct: 188 IQINAIAPGYIVTKNTEAIRNDESRNNEILSRIPAGKWGYPFDVMGTVVFLASRASDYIN 247 Query: 229 GAIVPVDGGYLV 240 G I+ VDGG+LV Sbjct: 248 GHILAVDGGWLV 259 >SMb21348 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Sme Sinorhizobium meliloti Length = 254 Score = 162 bits (410), Expect = 7e-40, Method: Compositional matrix adjust. Identities = 103/244 (42%), Positives = 138/244 (56%), Gaps = 12/244 (4%) Query: 8 LKGKTALVTGGTQGIGAAVARQLAALGARVIAAGLPPFAHEEF----AGSAVTLVEMNVA 63 L G+ +VTG GIG +A +A G VI G E AG ++A Sbjct: 9 LAGRRVVVTGANTGIGQGIAVSIARAGGTVIGVGRSAMDETEKKVTDAGGTFIPAHADLA 68 Query: 64 SEESVRAALAGLTQ----LDIVVNCAGIIKRGAELE-TEV-FEQVIAVNLTGTMRVCAA- 116 E+ R+ + GL L +VN AGII+R ++ TE ++ VI VNL + A Sbjct: 69 DREATRSLIDGLWNAHGPLQGLVNNAGIIRRADAVDFTEADWDDVIDVNLRSLFFLSQAF 128 Query: 117 -ARELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVA 175 R + +SRG IVN AS+LSF GG V Y+ASK G +T+ LA +AA GI VNAVA Sbjct: 129 GRRIIAEESRGKIVNIASLLSFQGGIRVASYTASKHGALGITRLLACEWAAKGINVNAVA 188 Query: 176 PGWIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVD 235 PG++ T T+AL+ D R+ IL R P GRWG P+D+G FL +PA+ +M GA+VPVD Sbjct: 189 PGYVVTNNTEALRADEKRSASILERIPAGRWGTPDDIGDAAVFLLAPASDYMHGAVVPVD 248 Query: 236 GGYL 239 GG+L Sbjct: 249 GGWL 252 >BH2167 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Bha Bacillus halodurans Length = 250 Score = 161 bits (407), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 100/243 (41%), Positives = 142/243 (58%), Gaps = 11/243 (4%) Query: 8 LKGKTALVTGGTQGIGAAVARQLAALGARVIAAGLPPFAH--EEFAGSAVTLVEMNV--- 62 L+GK ALVTG ++G+G +A LA GA VI AG+ + ++ T E+ Sbjct: 6 LEGKIALVTGASRGLGQGMAVGLAEAGADVIGAGISDMSETRQQIESLGRTFYELKADLF 65 Query: 63 ---ASEESVRAALAGLTQLDIVVNCAGIIKRG-AELETEV-FEQVIAVNLTGTMRVC-AA 116 A ++ + A+ ++DI+VN AGII+R AE T+ + VI VNL ++C A Sbjct: 66 EEGAGKQLIADAIEKAGRIDILVNNAGIIRRNEAEAFTDKDWNDVINVNLNAVFQMCREA 125 Query: 117 ARELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAP 176 ++ G I+N ASMLSF GG VP Y+ASK +A LTKSLA ++ G+ VNA+AP Sbjct: 126 GNHMIENGFGRIINIASMLSFQGGLRVPAYTASKHAIAGLTKSLANEWSKKGVNVNAIAP 185 Query: 177 GWIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDG 236 G++ T T AL+ D VR I +R P G WG P D+ V FL S A+S++ G I+ VDG Sbjct: 186 GYMVTDNTAALRADEVRNAYITSRIPKGEWGTPADLKGPVVFLASEASSYVNGHILCVDG 245 Query: 237 GYL 239 G++ Sbjct: 246 GWM 248 >AGl3337 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 247 Score = 160 bits (406), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 110/247 (44%), Positives = 148/247 (59%), Gaps = 17/247 (6%) Query: 5 PDLLKGKTALVTGGTQGIGAAVARQLAALGARVIAAG-------LPPFAHEEFAGSAVTL 57 P L+G+ ALVTG G+G A+A LAA GA V+ A L A + SA+ + Sbjct: 4 PFSLEGRKALVTGANTGLGQAIAVGLAAAGAEVVCAARRAPDETLDIIAKDGGNASALLI 63 Query: 58 VEMN-VASEESVRAALAGLTQLDIVVNCAGIIKRGAELE-TEV-FEQVIAVNLTGTMRVC 114 + +A+++S A DI+VN AGII+R +E +E+ +++V+ VNL Sbjct: 64 DFADPLAAKDSFTDA-----GFDILVNNAGIIRRADSVEFSELDWDEVMDVNLKALFFTT 118 Query: 115 AA-ARELLRQSR-GCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVN 172 A A+ELL + R G +VN AS+LSF GG VP Y+A+K GVA LTK LA +AA GI VN Sbjct: 119 QAFAKELLAKGRSGKVVNIASLLSFQGGIRVPSYTAAKHGVAGLTKLLANEWAAKGINVN 178 Query: 173 AVAPGWIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIV 232 A+APG+I T T+AL+ D R IL R P GRWG ED+ FL S AA ++ GAI+ Sbjct: 179 AIAPGYIETNNTEALRADAARNKAILERIPAGRWGHSEDIAGAAVFLSSAAADYVHGAIL 238 Query: 233 PVDGGYL 239 VDGG+L Sbjct: 239 NVDGGWL 245 >TM0441 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Tma Thermotoga maritima Length = 255 Score = 157 bits (397), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 103/249 (41%), Positives = 140/249 (56%), Gaps = 21/249 (8%) Query: 8 LKGKTALVTGGTQGIGAAVARQLAALGARVIAAGLPPFAHEEFAGSAVTLVEM------- 60 L+G+ ALVTGG++G+G +A+ LA G V+ A EE + +A L E Sbjct: 7 LRGRVALVTGGSRGLGFGIAQGLAEAGCSVVVASRN---LEEASEAAQKLTEKYGVETMA 63 Query: 61 ---NVASEESVRAALAGLTQ----LDIVVNCAGIIKR--GAELETEVFEQVIAVNLTGTM 111 +V++ E V+ L + + LD VVN AGI +R E + F QVI VNL GT Sbjct: 64 FRCDVSNYEEVKKLLEAVKEKFGKLDTVVNAAGINRRHPAEEFPLDEFRQVIEVNLFGTY 123 Query: 112 RVCAAARELLRQSRG-CIVNTASM-LSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGI 169 VC A LLR+S I+N S+ + + Y+ASKGGVA LTK+LA + GI Sbjct: 124 YVCREAFSLLRESDNPSIINIGSLTVEEVTMPNISAYAASKGGVASLTKALAKEWGRYGI 183 Query: 170 RVNAVAPGWIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTG 229 RVN +APGW T +T+A+ DP + +L R PLGR G PED+ V FL S A ++TG Sbjct: 184 RVNVIAPGWYRTKMTEAVFSDPEKLDYMLKRIPLGRTGVPEDLKGVAVFLASEEAKYVTG 243 Query: 230 AIVPVDGGY 238 I+ VDGG+ Sbjct: 244 QIIFVDGGW 252 >mll4054 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Mlo Mesorhizobium loti Length = 258 Score = 154 bits (390), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 104/245 (42%), Positives = 132/245 (53%), Gaps = 14/245 (5%) Query: 8 LKGKTALVTGGTQGIGAAVARQLAALGARVIAAGLPPFAHEEFAGSAVTLVEMNVASEES 67 L GK LVTG GIG + +A G VI G E + A S + Sbjct: 13 LAGKRILVTGANTGIGQGIVLSIARAGGVVIGVGRSSM-DETASKVAAIGARFETVSADL 71 Query: 68 VRAALAG--LTQ-------LDIVVNCAGIIKRG--AELETEVFEQVIAVNLTGTMRVCA- 115 A AG L + LD +VN AGII+R +L ++ V+ VNL +C Sbjct: 72 ADTATAGPMLDRVWDESGPLDGLVNNAGIIRRADAVDLTETDWDDVMDVNLKTVFLLCQS 131 Query: 116 -AARELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAV 174 A R L RG IVN AS+LSF GG V Y+ASK GV +T+ LA +AA GI VNA+ Sbjct: 132 FARRALADGRRGKIVNIASVLSFQGGIRVASYTASKHGVLGITRLLACEWAAKGINVNAI 191 Query: 175 APGWIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVPV 234 APG++ T TQAL+ DP R+ IL R P GRWG+P D+G FL + A+ +M GA VPV Sbjct: 192 APGYVETNNTQALRADPDRSAAILGRIPAGRWGEPSDIGDAAVFLLARASDYMHGAAVPV 251 Query: 235 DGGYL 239 DGG+L Sbjct: 252 DGGWL 256 >aq_1716 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Aae Aquifex aeolicus Length = 248 Score = 154 bits (389), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 101/244 (41%), Positives = 141/244 (57%), Gaps = 16/244 (6%) Query: 8 LKGKTALVTGGTQGIGAAVARQLAALGARVIAAGLP----PFAHEEFA---GSAVTLVEM 60 L+GK +LVTG T+GIG A+A +LA+ G+ VI G EE A G VEM Sbjct: 5 LQGKVSLVTGSTRGIGRAIAEKLASAGSTVIITGTSGERAKAVAEEIANKYGVKAHGVEM 64 Query: 61 NVASEESVRAALAGLTQL----DIVVNCAGIIKRGAELETEV--FEQVIAVNLTGTMRVC 114 N+ SEES+ A + L DI+VN AGI + L + +E+V+ VNLTGT V Sbjct: 65 NLLSEESINKAFEEIYNLVDGIDILVNNAGITRDKLFLRMSLLDWEEVLKVNLTGTFLVT 124 Query: 115 AAA-RELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNA 173 + R++++Q G IVN +S++ F G YS +K G+ TKSLA A + VNA Sbjct: 125 QNSLRKMIKQRWGRIVNISSVVGFTGNVGQVNYSTTKAGLIGFTKSLAKELAPRNVLVNA 184 Query: 174 VAPGWIATPLTQALQDDPVRAGPILARTPLGRWGQPEDVGQVVAFLCSPAASFMTGAIVP 233 VAPG+I T +T L ++ + + PLGR+G PE+V VV FLCS AS++TG ++ Sbjct: 185 VAPGFIETDMTAVLSEEIKQKYK--EQIPLGRFGSPEEVANVVLFLCSELASYITGEVIH 242 Query: 234 VDGG 237 V+GG Sbjct: 243 VNGG 246