BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0063.p (319 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0703 [R] COG0491 Zn-dependent hydrolases, including glyoxylas... 567 e-162 RSp1345 [R] COG0491 Zn-dependent hydrolases, including glyoxylas... 506 e-143 RSp1314 [R] COG0491 Zn-dependent hydrolases, including glyoxylas... 506 e-143 aq_1798 [R] COG0491 Zn-dependent hydrolases, including glyoxylas... 137 3e-32 SSO2519 [R] COG0491 Zn-dependent hydrolases, including glyoxylas... 113 6e-25 Rv0939_2 [R] COG0491 Zn-dependent hydrolases, including glyoxyla... 87 4e-17 MT0966_2 [R] COG0491 Zn-dependent hydrolases, including glyoxyla... 87 4e-17 AF0090 [R] COG0491 Zn-dependent hydrolases, including glyoxylase... 81 4e-15 aq_974 [R] COG0491 Zn-dependent hydrolases, including glyoxylase... 79 1e-14 AGpA282 [R] COG0491 Zn-dependent hydrolases, including glyoxylas... 74 3e-13 >RSp0703 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Rso Ralstonia solanacearum Length = 319 Score = 567 bits (1462), Expect = e-162, Method: Compositional matrix adjust. Identities = 267/318 (83%), Positives = 293/318 (92%) Query: 1 MAKAFASQADLEAKKISFVQLSDNAYAYTAEGDPNSGVIIGDDSVMVIDTTATPAMAQDL 60 MAKAFASQADLEAK+++F QL+ NAYAYTAEGDPNSGVIIGDDSV+++DTTATPAMAQDL Sbjct: 1 MAKAFASQADLEAKQVTFTQLAPNAYAYTAEGDPNSGVIIGDDSVLIVDTTATPAMAQDL 60 Query: 61 IARIRAITDKPIKYVVLSHYHAVRVLGASAYLQEGGEQVIASRGTYEMIVERGEADMKSE 120 IARIRAITDKPIK+VVLSHYHAVRVLGASAY EG + +IASRGTYEMIVERGEADMKSE Sbjct: 61 IARIRAITDKPIKHVVLSHYHAVRVLGASAYFAEGAQNIIASRGTYEMIVERGEADMKSE 120 Query: 121 IERFPRLFAGVETVPGLTWPTMVFEKEMTIFMGKLEVKIAHLGAGHTKGDTVVWIPSQKV 180 IERFPRLFAGVETVPGLTWPT+VFE+E+T+F+GKLEVKI HLG GHTKGDTVVWIPSQKV Sbjct: 121 IERFPRLFAGVETVPGLTWPTLVFERELTLFLGKLEVKILHLGPGHTKGDTVVWIPSQKV 180 Query: 181 LFSGDLVEYDAACYCGDAQLEQWPATLDALSALQAQALVPGRGPALVGREQVEQGIAYTR 240 LFSGDLVEYDAACYCGDAQLE+WPATLDAL AL + LVPGRGPAL +V+QG+AYTR Sbjct: 181 LFSGDLVEYDAACYCGDAQLEEWPATLDALRALGPEQLVPGRGPALRNPREVDQGLAYTR 240 Query: 241 DFVSTLLRAGKDAVAGKMSLKEAMAHTRSLMDPKFGQVFIYEHCLPFDVTRAFDEASGIT 300 DFVSTLL GK+AVA KM LK AMAHTR+ MDPKFG VFIYEHCLPFDVTRA+DEASGI Sbjct: 241 DFVSTLLARGKEAVAQKMDLKAAMAHTRAAMDPKFGHVFIYEHCLPFDVTRAYDEASGIK 300 Query: 301 HPRIWTAERDKEMWKALQ 318 HPRIWTA+RD++MW ALQ Sbjct: 301 HPRIWTAQRDQDMWAALQ 318 >RSp1345 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Rso Ralstonia solanacearum Length = 335 Score = 506 bits (1303), Expect = e-143, Method: Compositional matrix adjust. Identities = 232/318 (72%), Positives = 278/318 (87%) Query: 1 MAKAFASQADLEAKKISFVQLSDNAYAYTAEGDPNSGVIIGDDSVMVIDTTATPAMAQDL 60 M+K FAS D + K +F +LS+NAYAYTA GDPN+GVIIGDDSV+V+DTTATPAMAQDL Sbjct: 17 MSKTFASHGDTQVKATTFTRLSENAYAYTAAGDPNAGVIIGDDSVLVVDTTATPAMAQDL 76 Query: 61 IARIRAITDKPIKYVVLSHYHAVRVLGASAYLQEGGEQVIASRGTYEMIVERGEADMKSE 120 IA+IRA+TDKPI+++VLSHYHAVRVLGASA+L +G +Q+IASRGTY++IVERGE DM+SE Sbjct: 77 IAKIRAVTDKPIRHLVLSHYHAVRVLGASAFLAQGCQQIIASRGTYDLIVERGEQDMQSE 136 Query: 121 IERFPRLFAGVETVPGLTWPTMVFEKEMTIFMGKLEVKIAHLGAGHTKGDTVVWIPSQKV 180 IERFPRLFAGVET+PGLTWPT+VFE+ +T+++G LEV+IAHLGAGHTKGDTVVW+PSQKV Sbjct: 137 IERFPRLFAGVETIPGLTWPTLVFEQALTLWLGGLEVRIAHLGAGHTKGDTVVWLPSQKV 196 Query: 181 LFSGDLVEYDAACYCGDAQLEQWPATLDALSALQAQALVPGRGPALVGREQVEQGIAYTR 240 LFSGDLVE++AACYCGDA L+QWPATL+AL AL + LVPGRGPAL+ + I YTR Sbjct: 197 LFSGDLVEHEAACYCGDAHLDQWPATLEALRALGPEKLVPGRGPALLSARAADDAIRYTR 256 Query: 241 DFVSTLLRAGKDAVAGKMSLKEAMAHTRSLMDPKFGQVFIYEHCLPFDVTRAFDEASGIT 300 +FV+TL +AG++A A + LK AM H R+LMD +FG VFIYEHCLPFDV+RAFDEA GI Sbjct: 257 EFVTTLRKAGREAHAQGLDLKGAMRHARALMDARFGSVFIYEHCLPFDVSRAFDEAGGIA 316 Query: 301 HPRIWTAERDKEMWKALQ 318 HPRIWTA+RDKEMW ALQ Sbjct: 317 HPRIWTAQRDKEMWAALQ 334 >RSp1314 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Rso Ralstonia solanacearum Length = 335 Score = 506 bits (1303), Expect = e-143, Method: Compositional matrix adjust. Identities = 232/318 (72%), Positives = 278/318 (87%) Query: 1 MAKAFASQADLEAKKISFVQLSDNAYAYTAEGDPNSGVIIGDDSVMVIDTTATPAMAQDL 60 M+K FAS D + K +F +LS+NAYAYTA GDPN+GVIIGDDSV+V+DTTATPAMAQDL Sbjct: 17 MSKTFASHGDTQVKATTFTRLSENAYAYTAAGDPNAGVIIGDDSVLVVDTTATPAMAQDL 76 Query: 61 IARIRAITDKPIKYVVLSHYHAVRVLGASAYLQEGGEQVIASRGTYEMIVERGEADMKSE 120 IA+IRA+TDKPI+++VLSHYHAVRVLGASA+L +G +Q+IASRGTY++IVERGE DM+SE Sbjct: 77 IAKIRAVTDKPIRHLVLSHYHAVRVLGASAFLAQGCQQIIASRGTYDLIVERGEQDMQSE 136 Query: 121 IERFPRLFAGVETVPGLTWPTMVFEKEMTIFMGKLEVKIAHLGAGHTKGDTVVWIPSQKV 180 IERFPRLFAGVET+PGLTWPT+VFE+ +T+++G LEV+IAHLGAGHTKGDTVVW+PSQKV Sbjct: 137 IERFPRLFAGVETIPGLTWPTLVFEQALTLWLGGLEVRIAHLGAGHTKGDTVVWLPSQKV 196 Query: 181 LFSGDLVEYDAACYCGDAQLEQWPATLDALSALQAQALVPGRGPALVGREQVEQGIAYTR 240 LFSGDLVE++AACYCGDA L+QWPATL+AL AL + LVPGRGPAL+ + I YTR Sbjct: 197 LFSGDLVEHEAACYCGDAHLDQWPATLEALRALGPEKLVPGRGPALLSARAADDAIRYTR 256 Query: 241 DFVSTLLRAGKDAVAGKMSLKEAMAHTRSLMDPKFGQVFIYEHCLPFDVTRAFDEASGIT 300 +FV+TL +AG++A A + LK AM H R+LMD +FG VFIYEHCLPFDV+RAFDEA GI Sbjct: 257 EFVTTLRKAGREAHAQGLDLKGAMRHARALMDARFGSVFIYEHCLPFDVSRAFDEAGGIA 316 Query: 301 HPRIWTAERDKEMWKALQ 318 HPRIWTA+RDKEMW ALQ Sbjct: 317 HPRIWTAQRDKEMWAALQ 334 >aq_1798 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Aae Aquifex aeolicus Length = 326 Score = 137 bits (345), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 78/276 (28%), Positives = 141/276 (51%), Gaps = 27/276 (9%) Query: 35 NSGVIIGDDSVMVIDTTATPAMAQDLIARIRAITDKPIKYVVLSHYHAVRVLGASAYLQE 94 N+ + DD V+V+D +T + ++LI IR++T+KPI+++V++HYH GA A+ + Sbjct: 54 NAYFYVADDGVLVVDALSTYKLGKELIESIRSVTNKPIRFLVVTHYHTDHFYGAKAFREV 113 Query: 95 GGEQV--------IASRGTYEMIVERGEADMKSEIERFPRLFAGVETVPGLTWPTMVFEK 146 G E + I+ +Y + R + +K +E G E P PT+ K Sbjct: 114 GAEVIAHEWAFDYISQPSSYNFFLARKKI-LKEHLE-------GTELTP----PTITLTK 161 Query: 147 EMTIFM--GK--LEVKIAHLGAGHTKGDTVVWIPSQKVLFSGDLVEYDAACYCGDAQLEQ 202 + +++ GK ++ HL HT GD VVWIP +KVLFSGD+V + G Sbjct: 162 NLNVYLQVGKEYKRFEVLHLCRAHTNGDIVVWIPDEKVLFSGDIVFDGRLPFLGSGNSRT 221 Query: 203 WPATLDALSALQAQALVPGRGPALVGREQVEQGIAYTRDFVSTL---LRAGKDAVAGKMS 259 W LD + ++ + L+PG G AL+G +++++ +++TR ++ L +R + Sbjct: 222 WLVCLDEILKMKPRILLPGHGEALIGEKKIKEAVSWTRKYIKDLRETIRKLYEEGCDVEC 281 Query: 260 LKEAMAHTRSLMDPKFGQVFIYEHCLPFDVTRAFDE 295 ++E + +DP + QV ++ + P + + E Sbjct: 282 VRERINEELIKIDPSYAQVPVFFNVNPVNAYYVYFE 317 >SSO2519 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Sso Sulfolobus solfataricus Length = 308 Score = 113 bits (282), Expect = 6e-25, Method: Compositional matrix adjust. Identities = 76/248 (30%), Positives = 123/248 (49%), Gaps = 19/248 (7%) Query: 18 FVQLSDNAYAYT-AEGD---PNSGVIIGDDSVMVIDTTATPAMAQDLIARIRAITDKPIK 73 F ++SDN YA+ GD N+GVIIG + +V+D+ MA+ I+ I+ ITDKPIK Sbjct: 7 FERISDNVYAFIQPNGDWFLSNAGVIIGKNYAVVVDSLTNEKMARQFISEIKKITDKPIK 66 Query: 74 YVVLSHYHAVRVLGASAYLQEGGEQVIASRGTYEMIVERGEADMKSEIERFPRLFAGVET 133 Y++ +H H + + I + E +VE K I + LF ++ Sbjct: 67 YLINTHEHEDHLWTNHLF---PNAITICHKNCREKVVEGA----KRRINPYENLFNAIDF 119 Query: 134 VPGLTWPT-MVFEKEMTIFM-GKLEVKIAHLGAGHTKGDTVVWIPSQKVLFSGDLVEYDA 191 P +V + EM IF+ E+KIA++G HT D V+IP QKV+F+GDL+ + Sbjct: 120 SGYQYTPQEVVIKDEMRIFIDNDEEIKIAYVGYAHTTSDIYVYIPDQKVVFTGDLL-FSP 178 Query: 192 AC--YCGDAQLEQWPATLDALSALQAQALVPGRGPALVGREQVEQG---IAYTRDFVSTL 246 C + ++ + L+ L+ L A+ +PG G GR + + + + RD + Sbjct: 179 PCTPFALMGYIQGYINALENLANLDAEIFIPGHGEVSYGRNALYESRDYLIFVRDEARKI 238 Query: 247 LRAGKDAV 254 +R D + Sbjct: 239 MRTISDPI 246 >Rv0939_2 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Mtu Mycobacterium tuberculosis H37Rv Length = 344 Score = 87.4 bits (215), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 68/268 (25%), Positives = 126/268 (47%), Gaps = 32/268 (11%) Query: 13 AKKISFVQLSDNAYAYTAEGDPNSGVIIGDDSVMVIDTTATPAMAQDLIARIRAITDK-P 71 A ++ L D Y ++ N+G++ GD + +++DT A+ ++++A ++ +T++ P Sbjct: 40 ADRVWAWTLPDGGYGFS-----NAGLVAGDGASLLVDTLFDLALTREMLAAMKPVTERAP 94 Query: 72 IKYVVLSHYHAVRVLGASAYLQEGGEQVIASRGTYEMIVERGEADMKSEIE--------- 122 I +++H + G L + ++IA++GT E I +M + I+ Sbjct: 95 ITDALITHSNGDHTHGTQ--LLDRSVRIIAAKGTSEEIEHGPAPEMLARIQTADLGPVAT 152 Query: 123 -----RFPRL-FAGVETVPGLTWPTMVFEKEMTIFMGKLEVKIAHLGAGHTKGDTVVWIP 176 RF F+G++ L + F++++ I +G V + +LG HT D+VV + Sbjct: 153 RYLRDRFGHFDFSGIK----LRNADLTFDRDLAIELGGRRVDLLNLGPAHTTADSVVHVA 208 Query: 177 SQKVLFSGDLVEYDAACYCGDAQLEQWPATLDALSALQAQALVPGRGPALVGREQVEQGI 236 VLF+GDL+ + W A DA+ AL A +VPG GP GI Sbjct: 209 DAGVLFAGDLLFIGCTPIVWAGPIANWVAACDAMIALDAPTVVPGHGPV-----TGPDGI 263 Query: 237 AYTRDFVSTLLRAGKDAVAGKMSLKEAM 264 R +++ + + A +SL EA+ Sbjct: 264 RAVRGYLAHIAEQAEAAYRKGLSLPEAV 291 >MT0966_2 [R] COG0491 Zn-dependent hydrolases, including glyoxylases MtC Mycobacterium tuberculosis CDC1551 Length = 342 Score = 87.4 bits (215), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 68/268 (25%), Positives = 126/268 (47%), Gaps = 32/268 (11%) Query: 13 AKKISFVQLSDNAYAYTAEGDPNSGVIIGDDSVMVIDTTATPAMAQDLIARIRAITDK-P 71 A ++ L D Y ++ N+G++ GD + +++DT A+ ++++A ++ +T++ P Sbjct: 38 ADRVWAWTLPDGGYGFS-----NAGLVAGDGASLLVDTLFDLALTREMLAAMKPVTERAP 92 Query: 72 IKYVVLSHYHAVRVLGASAYLQEGGEQVIASRGTYEMIVERGEADMKSEIE--------- 122 I +++H + G L + ++IA++GT E I +M + I+ Sbjct: 93 ITDALITHSNGDHTHGTQ--LLDRSVRIIAAKGTSEEIEHGPAPEMLARIQTADLGPVAT 150 Query: 123 -----RFPRL-FAGVETVPGLTWPTMVFEKEMTIFMGKLEVKIAHLGAGHTKGDTVVWIP 176 RF F+G++ L + F++++ I +G V + +LG HT D+VV + Sbjct: 151 RYLRDRFGHFDFSGIK----LRNADLTFDRDLAIELGGRRVDLLNLGPAHTTADSVVHVA 206 Query: 177 SQKVLFSGDLVEYDAACYCGDAQLEQWPATLDALSALQAQALVPGRGPALVGREQVEQGI 236 VLF+GDL+ + W A DA+ AL A +VPG GP GI Sbjct: 207 DAGVLFAGDLLFIGCTPIVWAGPIANWVAACDAMIALDAPTVVPGHGPV-----TGPDGI 261 Query: 237 AYTRDFVSTLLRAGKDAVAGKMSLKEAM 264 R +++ + + A +SL EA+ Sbjct: 262 RAVRGYLAHIAEQAEAAYRKGLSLPEAV 289 >AF0090 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Afu Archaeoglobus fulgidus Length = 293 Score = 80.9 bits (198), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 66/236 (27%), Positives = 110/236 (46%), Gaps = 35/236 (14%) Query: 17 SFVQLSDNAYAYT-AEGD---PNSGVIIGDDSVMVIDTTATPAMAQDLIARIRAITDKPI 72 V+++D YAY G+ N+G+I+G++ +V+D+ A+++I++ R +T K Sbjct: 4 KLVEVADGVYAYIQGRGEWFVNNAGLIVGEEFSVVVDSVCNEQRAKEMISQFREVTAKDF 63 Query: 73 KYVVLSHYHAVRV-----LGASAYLQEGGEQ--VIASRGTYEMIVERGEADMKSEIERFP 125 + ++ +H H V A A E G Q ++A Y+ + FP Sbjct: 64 RILINTHGHPDHVWTNHLFDAVAVAHEMGRQETLMAFVEIYQSL--------------FP 109 Query: 126 RL-FAGVETVPGLTWPTMVFEKEMTIFMGKLEVKIAHLGAGHTKGDTVVWIPSQKVLFSG 184 L F+G + P + FE M ++ E++I H G HT+GD ++IP +KV+F G Sbjct: 110 DLDFSGAKITP----QNITFESRMKLY-NDAEMQIIHPGVAHTRGDCYIYIPQKKVVFCG 164 Query: 185 DLVEYDAAC--YCGDAQLEQWPATLDALSALQAQALVPGRGPALVGREQVEQGIAY 238 DL+ + C + L L L A+ VPG G L G E + + I Y Sbjct: 165 DLL-FAKPCTPLAVGGSVRGSVNALRELLNLDAKIYVPGHG-GLAGEEHLVEAIEY 218 >aq_974 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Aae Aquifex aeolicus Length = 307 Score = 79.3 bits (194), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 63/234 (26%), Positives = 119/234 (50%), Gaps = 12/234 (5%) Query: 35 NSGVIIGDDSVMVIDTTATPAMAQDLIARIRAITDKPIKYVVLSHYHAVRVLGASAYLQE 94 N+ ++ ++ +VIDT TP ++++ + ++ +++KP+ Y +++HYH GA + ++ Sbjct: 52 NAYAVLTEEGWVVIDTLTTPELSEEFLNLLKKVSNKPVIYAIVTHYHLDHWYGAKTF-KD 110 Query: 95 GGEQVIASRGTYEMIVERGEA--DMKSEIERFPRLFAGVETVPGLTWPTMVFEKEMTIFM 152 G +VIA E + GEA ++++ +RF + VE VP P V + + + + Sbjct: 111 KGAKVIAHEKLKEF-YDSGEALQVLEAQKKRFKGVLDSVELVP----PDEVVKDKKVLKV 165 Query: 153 GKLEVKIAHLGAGHTKGDTVVWIPSQKVLFSGDLVEYDAACYCGD--AQLEQWPATLDAL 210 GK +I + HT D V++ ++KVLF GDLV + + GD A + W L+ L Sbjct: 166 GKDVFEIYAMPPAHTNSDLVIYWKNRKVLFVGDLVYINRIPFMGDRNASSKGWLEVLERL 225 Query: 211 SALQAQALVPGRGPALVGREQVEQGIAYTRDFVSTLLRAGKDAVAGKMSLKEAM 264 A+ L+ G + ++ ++ Y + FV ++ KD +KEA Sbjct: 226 KEFDARMLLGGHNYPM-NKDAIDWTYNYIK-FVRDTVKKLKDEGYFIDEIKEAF 277 >AGpA282 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 359 Score = 74.3 bits (181), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 58/243 (23%), Positives = 109/243 (44%), Gaps = 32/243 (13%) Query: 25 AYAYTAEGDPNSGVIIGDDSVMVIDTTATPAMAQDLIARIRAITDKPIKYVVLSHYHAVR 84 + + G ++ ++ D V+ ID A P + ++++A I +TDKP+ +++ SH+H Sbjct: 61 GFYWVTSGGYDAAFVVTSDGVVAID--APPTIGENMLAAIEDVTDKPVTHLIYSHWHTDH 118 Query: 85 VLGASAYLQEGGEQVIASRGTYEMIVERGEADMKSEIERFPRLFAGVETVPGLTWPTMVF 144 + AS + +++A T E++ ERFP P PT+ F Sbjct: 119 IGAASVFGPN--VEIVAHDITKELL------------ERFPD--------PNRPVPTVTF 156 Query: 145 EKEMTIFMGKLEVKIAHLGAGHTKGDTVVWIPSQKVLFSGDLVEYDAACY--CGDAQ-LE 201 +K+ + +G + +++++ G H G+ ++ P+QKVL D+V +A + C +Q + Sbjct: 157 DKDHLLVVGGVTLELSYKGENHCPGNIFIYAPAQKVLTVIDIVSPGSATFMHCDASQNIT 216 Query: 202 QWPATLDALSALQAQALVPGRGPALVGREQVEQGIAYTRDFVSTLLRAGKDAVAGKMSLK 261 W L + LV G E V I Y +D + G A + S Sbjct: 217 GWYEAQKQLMEYEFDFLVAGHHMKYGTPETVAASIEYFQDVLD-----GAQAAVDRFSRS 271 Query: 262 EAM 264 +A+ Sbjct: 272 DAL 274