BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0026.p (199 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value YER163c [P] COG3703 Uncharacterized protein involved in cation t... 112 5e-25 SPBC31F10.03 [P] COG3703 Uncharacterized protein involved in cat... 110 2e-24 AGl2432 [P] COG3703 Uncharacterized protein involved in cation t... 84 3e-16 SMc00713 [P] COG3703 Uncharacterized protein involved in cation ... 78 2e-14 BMEI0077 [P] COG3703 Uncharacterized protein involved in cation ... 77 2e-14 chaC [P] COG3703 Uncharacterized protein involved in cation tran... 77 3e-14 ZchaC [P] COG3703 Uncharacterized protein involved in cation tra... 76 4e-14 ECs1723 [P] COG3703 Uncharacterized protein involved in cation t... 76 4e-14 mll3532 [P] COG3703 Uncharacterized protein involved in cation t... 74 3e-13 CC2220 Non COG 74 3e-13 >YER163c [P] COG3703 Uncharacterized protein involved in cation transport Sce Saccharomyces cerevisiae Length = 232 Score = 112 bits (280), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 76/222 (34%), Positives = 105/222 (47%), Gaps = 51/222 (22%) Query: 16 DDHASVWLFGYGSIIFKADFPYLEAGPAHIKGWARRFWQGSHDHRGTAEAPGRVVTLVAQ 75 +D++ +W+ GYGS+I+K Y PA I G+ARRFWQ S DHRGT PGRV TL+ Sbjct: 3 NDNSGIWVLGYGSLIYKPPSHYTHRIPAIIHGFARRFWQSSTDHRGTPANPGRVATLIPY 62 Query: 76 EDAVCE-------------------------GMAYLITP----EVFAHLDHREKNGYLRL 106 ED + + G+ Y I P EV +L+ RE+NGY Sbjct: 63 EDIIRQTAFLKNVNLYSESAPIQDPDDLVTIGVVYYIPPEHAQEVREYLNVREQNGYTLH 122 Query: 107 VTPILFGS--------GQSAEGL--------------IYIASEENEAFAGPASELEIARQ 144 + + G++ E L +YI + +NEAF GP + E A+ Sbjct: 123 EVEVHLETNREHEAELGEALEQLPRHNKSGKRVLLTSVYIGTIDNEAFVGPETVDETAKV 182 Query: 145 IAHAAGPSGRNSEYLLKLAQALRELGAQDRHVFEIEHHLLQL 186 IA + GPSG N EYL KL QAL ++ +H+L L Sbjct: 183 IAVSHGPSGSNYEYLAKLEQALAQMPIMKERGRITDHYLTAL 224 >SPBC31F10.03 [P] COG3703 Uncharacterized protein involved in cation transport Spo Schizosaccharomyces pombe Length = 203 Score = 110 bits (275), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 68/192 (35%), Positives = 97/192 (50%), Gaps = 23/192 (11%) Query: 12 MRHFDDHASVWLFGYGSIIFKADFPYLEAGPAHIKGWARRFWQGSHDHRGTAEAPGRVVT 71 M+ S+W+FGYGS+I+ Y + P IKG+ RRFW S DHRGT +PG V+T Sbjct: 1 MKTLSPEGSLWVFGYGSLIWHPPPHYDYSIPCFIKGYVRRFWMRSEDHRGTVNSPGLVLT 60 Query: 72 LVAQE-------------DAVCEGMAYLI----TPEVFAHLDHREKNGYLRLVTPILFGS 114 L+ E D C GMA+ I +V +LD RE NGY P+ + Sbjct: 61 LIPYEEWKQFSDWSFTPFDEGCWGMAFRIPAKYATQVREYLDDREVNGYTAHSVPVYAHT 120 Query: 115 GQSA----EGLIYIASEENEAFAGPASELEIARQIAHAAGPSGRNSEYLLKLAQALRELG 170 G L+Y+ + ++ F ++A+ I+ G SG N YL +LA+ LR L Sbjct: 121 GDEIPVLENCLVYVGTSKSPQFQPSDDLTQMAKIISTRRGKSGDNFVYLFELAKCLRHLS 180 Query: 171 --AQDRHVFEIE 180 ++D HVFE+E Sbjct: 181 PESKDIHVFELE 192 >AGl2432 [P] COG3703 Uncharacterized protein involved in cation transport Atu Agrobacterium tumefaciens strain C58 (Cereon) Length = 219 Score = 83.6 bits (205), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 54/163 (33%), Positives = 81/163 (49%), Gaps = 8/163 (4%) Query: 17 DHASVWLFGYGSIIFKADFPYLEAGPAHIKGWARRFWQGSHDHRGTAEAPGRVVTLVAQE 76 D W+FGYGS+++ F + E A + G+ R S+ +RGT E PG V+ L + Sbjct: 36 DMDDFWVFGYGSLMWNPGFAFEERQQARLHGYRRSLCISSNFYRGTEEKPGLVLGL--ER 93 Query: 77 DAVCEGMAYLITPE----VFAHLDHRE--KNGYLRLVTPILFGSGQSAEGLIYIASEENE 130 C G+A+ + + V A+L RE N Y V I +G+ + Y+A +E Sbjct: 94 GGSCLGVAFRVRGKDHDPVMAYLRERELVTNVYKERVVSIALANGRRGSAVTYVADPAHE 153 Query: 131 AFAGPASELEIARQIAHAAGPSGRNSEYLLKLAQALRELGAQD 173 + G E A IA A G SG N++YL Q L+E+G +D Sbjct: 154 QYIGGLGVAESAVIIAAATGRSGPNTDYLFNTVQHLQEMGIRD 196 >SMc00713 [P] COG3703 Uncharacterized protein involved in cation transport Sme Sinorhizobium meliloti Length = 184 Score = 77.8 bits (190), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 58/173 (33%), Positives = 81/173 (46%), Gaps = 8/173 (4%) Query: 17 DHASVWLFGYGSIIFKADFPYLEAGPAHIKGWARRFWQGSHDHRGTAEAPGRVVTLVAQE 76 D W+FGYGS+++ F + E A G+ R S HRGT PG V+ L Sbjct: 4 DMDEFWVFGYGSLMWNPGFRFEEKLTARAFGYRRSLCVRSWVHRGTERHPGLVLGL--DY 61 Query: 77 DAVCEGMAYLITP----EVFAHLDHRE--KNGYLRLVTPILFGSGQSAEGLIYIASEENE 130 C G A+ + P EV +L RE + Y P+ SGQ L Y+ + Sbjct: 62 GGSCIGTAFRVAPAQKAEVIDYLRERELVTHVYKERTMPVQLSSGQRVSALTYVIDRSHV 121 Query: 131 AFAGPASELEIARQIAHAAGPSGRNSEYLLKLAQALRELGAQDRHVFEIEHHL 183 +AG S E A +A A G SG NSEY+L + LR++G +D + E+ L Sbjct: 122 QYAGALSAEEAAATVAVATGKSGPNSEYVLNTLEHLRDMGIRDHWLEEVAAKL 174 >BMEI0077 [P] COG3703 Uncharacterized protein involved in cation transport Bme Brucella melitensis Length = 190 Score = 77.0 bits (188), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 57/179 (31%), Positives = 85/179 (47%), Gaps = 24/179 (13%) Query: 12 MRHFDDHASVWLFGYGSIIFKADFPYLEAGPAHIKGWARRFWQGSHDHRGTAEAPGRVVT 71 M H +D W+FGYGS++++ F ++E A + G+ R SH HRGT + PG V+ Sbjct: 6 MEHRND---FWVFGYGSLMWRPGFAHVETVRARLHGYRRSLCIYSHVHRGTPDHPGLVLG 62 Query: 72 LVAQEDAVCEGMAYLI----TPEVFAHLDHR--------EKNGYLRLVTPILFGSGQSAE 119 L C G+A+ + T EV +L R EK +LRL G+ E Sbjct: 63 L--DTGGSCLGIAFRVPGDMTDEVMTYLREREMANKVYHEKKLHLRL------ADGRIVE 114 Query: 120 GLIYIASEENEAFAGPASELEIARQIAHAAGPSGRNSEYLLKLAQALRELGAQDRHVFE 178 + Y+A + +AG + + A IA A G SG N +Y+ LR + +D H E Sbjct: 115 AVAYVADRRHRQYAGRMTSEKAAAIIASAQGESGANIDYVTNTLMHLRNMRVRD-HALE 172 >chaC [P] COG3703 Uncharacterized protein involved in cation transport Eco Escherichia coli K12 Length = 238 Score = 76.6 bits (187), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 51/163 (31%), Positives = 75/163 (46%), Gaps = 8/163 (4%) Query: 17 DHASVWLFGYGSIIFKADFPYLEAGPAHIKGWARRFWQGSHDHRGTAEAPGRVVTLVAQE 76 D VW+FGYGS+++ + E+ + GW R F RGTA PGR++ L +E Sbjct: 50 DEGPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLAL--KE 107 Query: 77 DAVCEGMAYLITPEVFAH----LDHRE--KNGYLRLVTPILFGSGQSAEGLIYIASEENE 130 G+AY + E L RE YL + G++ +++I + Sbjct: 108 GGRTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP 167 Query: 131 AFAGPASELEIARQIAHAAGPSGRNSEYLLKLAQALRELGAQD 173 + IA IA A+GP G N++YL L Q L +LG QD Sbjct: 168 EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQD 210 >ZchaC [P] COG3703 Uncharacterized protein involved in cation transport EcZ Escherichia coli O157:H7 EDL933 Length = 231 Score = 76.3 bits (186), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 51/163 (31%), Positives = 75/163 (46%), Gaps = 8/163 (4%) Query: 17 DHASVWLFGYGSIIFKADFPYLEAGPAHIKGWARRFWQGSHDHRGTAEAPGRVVTLVAQE 76 D VW+FGYGS+++ + E+ + GW R F RGTA PGR++ L +E Sbjct: 43 DERPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLAL--KE 100 Query: 77 DAVCEGMAYLITPEVFAH----LDHRE--KNGYLRLVTPILFGSGQSAEGLIYIASEENE 130 G+AY + E L RE YL + G++ +++I + Sbjct: 101 GGRTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP 160 Query: 131 AFAGPASELEIARQIAHAAGPSGRNSEYLLKLAQALRELGAQD 173 + IA IA A+GP G N++YL L Q L +LG QD Sbjct: 161 EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQD 203 >ECs1723 [P] COG3703 Uncharacterized protein involved in cation transport Ecs Escherichia coli O157:H7 Length = 239 Score = 76.3 bits (186), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 51/163 (31%), Positives = 75/163 (46%), Gaps = 8/163 (4%) Query: 17 DHASVWLFGYGSIIFKADFPYLEAGPAHIKGWARRFWQGSHDHRGTAEAPGRVVTLVAQE 76 D VW+FGYGS+++ + E+ + GW R F RGTA PGR++ L +E Sbjct: 51 DERPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLAL--KE 108 Query: 77 DAVCEGMAYLITPEVFAH----LDHRE--KNGYLRLVTPILFGSGQSAEGLIYIASEENE 130 G+AY + E L RE YL + G++ +++I + Sbjct: 109 GGRTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP 168 Query: 131 AFAGPASELEIARQIAHAAGPSGRNSEYLLKLAQALRELGAQD 173 + IA IA A+GP G N++YL L Q L +LG QD Sbjct: 169 EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQD 211 >mll3532 [P] COG3703 Uncharacterized protein involved in cation transport Mlo Mesorhizobium loti Length = 184 Score = 73.6 bits (179), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 55/168 (32%), Positives = 81/168 (48%), Gaps = 9/168 (5%) Query: 12 MRHFDDHASVWLFGYGSIIFKADFPYLEAGPAHIKGWARRFWQGSHDHRGTAEAPGRVVT 71 +RH D W+FGYGS+I++ F ++E AH+ G+ R S HRGT E PG V+ Sbjct: 7 IRH-GDMGDFWVFGYGSLIWRPGFAHVETRRAHLHGYRRSLCVYSFVHRGTRERPGLVLG 65 Query: 72 LVAQEDAVCEGMAYLI----TPEVFAHLDHRE--KNGYLRLVTPILFGSGQSAEGLIYIA 125 L C G+A+ + EV A+L RE + YL + G + E + YI Sbjct: 66 L--DHGGSCVGLAFRVPGELRDEVIAYLRERELVTSVYLERALKVRLDGGGTVEAVAYIV 123 Query: 126 SEENEAFAGPASELEIARQIAHAAGPSGRNSEYLLKLAQALRELGAQD 173 ++E +AG A + A G SG N +Y+ + L LG +D Sbjct: 124 DRKHEQYAGALDAAHAAAVVRGAVGQSGNNEDYVFSTLKHLEALGIRD 171 >CC2220 Non COG Length = 182 Score = 73.6 bits (179), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 52/158 (32%), Positives = 78/158 (49%), Gaps = 8/158 (5%) Query: 22 WLFGYGSIIFKADFPYLEAGPAHIKGWARRFWQGSHDHRGTAEAPGRVVTLVAQEDAVCE 81 W+FGYGS++++ FP+++ A + G R F S HRGT E PG V+ L Sbjct: 11 WVFGYGSLMWRPGFPFIDRRTAVLHGRRRAFCIYSVHHRGTYERPGLVLGLAP--GGSVR 68 Query: 82 GMAYLITPE----VFAHLDHREK--NGYLRLVTPILFGSGQSAEGLIYIASEENEAFAGP 135 GMAY + V+A+L RE+ Y + G A L++++ ++ +AG Sbjct: 69 GMAYRVAAAEWEGVYAYLREREQPTETYFETWREVKVDGGDKAPALVFLSDMKHSQWAGD 128 Query: 136 ASELEIARQIAHAAGPSGRNSEYLLKLAQALRELGAQD 173 + + A IA A G SG N +YL L L E G +D Sbjct: 129 LTLEQQAALIAGAVGLSGPNIDYLRDLVMHLHEDGVRD 166