BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0005.0022.p (282 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc3346 [I] COG2267 Lysophospholipase Rso Ralstonia solanacearum 207 1e-53 AF1753 [I] COG2267 Lysophospholipase Afu Archaeoglobus fulgidus 129 1e-29 TM1350 [I] COG2267 Lysophospholipase Tma Thermotoga maritima 124 3e-28 PAB1050 [I] COG2267 Lysophospholipase Pab Pyrococcus abyssi 116 6e-26 BH3288 [I] COG2267 Lysophospholipase Bha Bacillus halodurans 112 8e-25 Rv0183 [I] COG2267 Lysophospholipase Mtu Mycobacterium tuberculo... 107 3e-23 MT0192 [I] COG2267 Lysophospholipase MtC Mycobacterium tuberculo... 107 3e-23 DR1537 [I] COG2267 Lysophospholipase Dra Deinococcus radiodurans 106 5e-23 BS_ytpA [I] COG2267 Lysophospholipase Bsu Bacillus subtilis 95 2e-19 ML2603 [I] COG2267 Lysophospholipase Mle Mycobacterium leprae 94 3e-19 >RSc3346 [I] COG2267 Lysophospholipase Rso Ralstonia solanacearum Length = 286 Score = 207 bits (528), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 116/266 (43%), Positives = 152/266 (57%), Gaps = 6/266 (2%) Query: 16 DGTRLFCRDWLLPGATA-----AVQMVHGLGEHGGRYFSLARLFNEAGLSVRICDQRGHG 70 DGT LF R WL P A V +VHG+ EH GRY +A++ E GL VR D RGHG Sbjct: 19 DGTELFVRTWL-PAPEAGEPRGTVILVHGMAEHSGRYPHVAQVLCELGLRVRAFDLRGHG 77 Query: 71 NSGGVQGSLIRPDDLLRDLKLSFDDFSRRTECVPVLFGHSMGGLVAARFATGGYSPVRAL 130 SGG + +L PD+ L DL D +P + GHSMGGL+ ARFAT PVR + Sbjct: 78 RSGGPRMALDAPDNYLTDLAEILDAVVAEWNEMPFVLGHSMGGLIVARFATARVRPVRGV 137 Query: 131 ALSSPALALDMKGWQKLLLAVSTAIAPGLALPTALPASRISHDPHEVRAYRKDPLNHGKI 190 LSSPAL L + +L + +A+AP L +P + +R+SHDP AYR DP I Sbjct: 138 LLSSPALRLKLPPGANAVLGLLSALAPKLPVPNPVDPARLSHDPSVGAAYRADPQVQKTI 197 Query: 191 APRMLHFMLDAMRQVEREAGQFTRPVLLQVAGDDAFVAPRGSRAFFDALPSGRKTLHWYE 250 + +L FML+A+ Q +++A + P+LL G D V P GSR F P +TL W+E Sbjct: 198 SASVLAFMLNAITQAQQDAPRLEAPMLLLAGGADTIVDPSGSRDFCAGAPEDLRTLAWFE 257 Query: 251 HAYHEIFNEEASLRAQAMQDLRNWLA 276 AYHE+FNE +R +A +R WLA Sbjct: 258 TAYHELFNEAEPMRGEAFGTMRAWLA 283 >AF1753 [I] COG2267 Lysophospholipase Afu Archaeoglobus fulgidus Length = 266 Score = 129 bits (323), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 90/265 (33%), Positives = 129/265 (48%), Gaps = 8/265 (3%) Query: 16 DGTRLFCRDWLLPGATAAVQMVHGLGEHGGRYFSLARLFNEAGLSVRICDQRGHGNSGGV 75 DG L+ R W + A + +VHGLGEH GRY +AR FNE G+S D RGHG S G Sbjct: 7 DGLTLYTRRWDVESPRAVICLVHGLGEHSGRYEHVARFFNENGISFAAFDLRGHGRSEGK 66 Query: 76 QGSLIRPDDLLRDLKLSFDDFSRRTECVPVLFGHSMGGLVAARFATGGYSPVRALALSSP 135 +G L+ D+ L S +C +L+GHSMGG +A + + +S+P Sbjct: 67 RGH-AEYQQLMDDITLFLQ--SLDYDCPKILYGHSMGGNLALNYILRYDPDIAGGIISAP 123 Query: 136 ALALDMKGWQKL--LLAVSTAIAPGLALPTALPASRISHDPHEVRAYRKDPLNHGKIAPR 193 LAL + + L +L + +AP + L + + IS D V AY DPL H KI+PR Sbjct: 124 FLALPKELPKHLFFILKLLNVVAPSIQLSNGIDPNLISRDREVVEAYVSDPLVHDKISPR 183 Query: 194 MLHFMLDAMRQVEREAGQFTRPVLLQVAGDDAFVAPRGSRAFFDALPSGRKTLHWYEHAY 253 + L+A + A + +P+LL D + R S+ F K + YE Y Sbjct: 184 FILQSLEAGKWALENADRLRKPILLIHGTADQITSYRASQEFAKRAGELCKFVS-YEGFY 242 Query: 254 HEIFNEEASLRAQAMQDLRNWLAQL 278 HE NE R A D+ W+ ++ Sbjct: 243 HEPHNEPEKERVLA--DMLKWIEEV 265 >TM1350 [I] COG2267 Lysophospholipase Tma Thermotoga maritima Length = 259 Score = 124 bits (310), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 89/255 (34%), Positives = 130/255 (50%), Gaps = 18/255 (7%) Query: 24 DWLLPGATAAVQMVHGLGEHGGRYFSLARLFNEAGLSVRICDQRGHGNSGGVQGSLIRPD 83 DW V +VHGLGEH GRY L R F G+ V D GHG S G +G L R D Sbjct: 11 DW-----KGTVVIVHGLGEHSGRYRRLVREFVSEGVQVVTFDLPGHGKSPGRRGHL-RFD 64 Query: 84 DLLRDLKLSFDDFSRRTECVPVLFGHSMGGLVAARFATGGYSPV--RALALSSPALAL-- 139 D+ + L D R VLFGHS+GGL+A RF T + P + L +S+PA+ L Sbjct: 65 DVFKILNEITKDLERF-----VLFGHSLGGLIAIRF-TQIFQPENQKGLVVSAPAILLPD 118 Query: 140 DMKGWQKLLLAVSTAIAPGLALPTALPASRISHDPHEVRAYRKDPLNHGKIAPRMLHFML 199 + ++ + P L + + S +S + V AY +DPL H +I+ ++ ML Sbjct: 119 THSPVLEFMVRFLSFFVPFLTMSNGINPSDLSRNREAVEAYIRDPLVHDRISFKLASDML 178 Query: 200 DAMRQVEREAGQFTRPVLLQVAGDDAFVAPRGSRAFFDALPSGRKTLHWYEHAYHEIFNE 259 M++V ++A + PVL+ DD V+ GS+ FF+AL S K L + YHE+F E Sbjct: 179 SHMKKVLKDAERIKVPVLIFHGTDDRVVSFEGSKKFFEAL-STEKKLVSFPGGYHELF-E 236 Query: 260 EASLRAQAMQDLRNW 274 + + + + + W Sbjct: 237 DPEHQKEFFKTIVEW 251 >PAB1050 [I] COG2267 Lysophospholipase Pab Pyrococcus abyssi Length = 259 Score = 116 bits (290), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 72/246 (29%), Positives = 117/246 (47%), Gaps = 8/246 (3%) Query: 34 VQMVHGLGEHGGRYFSLARLFNEAGLSVRICDQRGHGNSGGVQGSLIRPDDLLRDLKLSF 93 V +VHGLGEH GRY + + G V D GHG S G +G + +K+ Sbjct: 17 VVLVHGLGEHIGRYEKFIQELVKNGFCVVGFDWPGHGKSKGKRGH----TSVEEAMKI-I 71 Query: 94 DDFSRRTECVPVLFGHSMGGLVAARFATGGYSPVRALALSSPALALDMK--GWQKLLLAV 151 D+ P LFGHS+GGL R+A ++ + SSPALA + G+ + + Sbjct: 72 DEIISEIGEKPFLFGHSLGGLTVIRYAEERGEKIKGVVASSPALAKSPRTPGFMVAIAKI 131 Query: 152 STAIAPGLALPTALPASRISHDPHEVRAYRKDPLNHGKIAPRMLHFMLDAMRQVEREAGQ 211 I P ++ + +S +P V+ Y +DPL H +++ ++ + M + R+A + Sbjct: 132 LGRILPSVSFSNGIDPELLSRNPERVKRYVEDPLVHDRVSAKLGMSIFKNMEEAHRKAEK 191 Query: 212 FTRPVLLQVAGDDAFVAPRGSRAFFDALPSGRKTLHWYEHAYHEIFNEEASLRAQAMQDL 271 P+L+ V D P G++ F++ L K L + AYHEIF E+ + + + Sbjct: 192 IKVPILILVGSGDVITPPEGAKRFYERLKVEDKKLVEFPGAYHEIF-EDPEFGKEFEETI 250 Query: 272 RNWLAQ 277 WL + Sbjct: 251 IKWLVE 256 >BH3288 [I] COG2267 Lysophospholipase Bha Bacillus halodurans Length = 260 Score = 112 bits (280), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 80/242 (33%), Positives = 123/242 (50%), Gaps = 9/242 (3%) Query: 24 DWLLPGATAAVQMVHGLGEHGGRYFSLARLFNEAGLSVRICDQRGHGNSGGVQGSLIRPD 83 W + V ++HG GEH GRY LA+ FN GLSV + D G G + G +G + Sbjct: 3 KWEVAEPRGVVVVIHGAGEHHGRYQWLAKKFNSIGLSVVMGDLPGQGRTRGKRGHIQSFQ 62 Query: 84 DLLRDLKLSFDDFSRRTECVPV-LFGHSMGGLVAAR-FATGGYSPVRALALSSPALALDM 141 + D+ L + + + + E VP+ LFGHSMGGLVA R GG PVRA+ LSSP L Sbjct: 63 QYI-DVVLEWVE-AAKLEHVPIFLFGHSMGGLVAVRTMIEGGTLPVRAVILSSPCFDLYQ 120 Query: 142 ---KGWQKLLLAVSTAIAPGLALPTALPASRISHDPHEVRAYRKDPLNHGKIAPRMLHFM 198 KG ++L + + P + + + + ++ + AY KD L K++ + + + Sbjct: 121 SPGKG-KELASKMLHRVTPTFSHHSGIRSDLVTRNEEIREAYLKDELRVTKVSTKWYYEL 179 Query: 199 LDAMRQVEREAGQF-TRPVLLQVAGDDAFVAPRGSRAFFDALPSGRKTLHWYEHAYHEIF 257 AMR R +F P+L+ AG+D + + +F+++ K + YHEIF Sbjct: 180 SKAMRDTRRYPEKFPNVPLLVMQAGEDYITDRKAAWEWFNSVQVTEKAYKEWNGLYHEIF 239 Query: 258 NE 259 NE Sbjct: 240 NE 241 >Rv0183 [I] COG2267 Lysophospholipase Mtu Mycobacterium tuberculosis H37Rv Length = 323 Score = 107 bits (268), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 86/261 (32%), Positives = 121/261 (46%), Gaps = 29/261 (11%) Query: 32 AAVQMVHGLGEHGGRYFSLARLFNEAGLSVRICDQRGHGNSGGVQGSLIRPDDLLRDLKL 91 A V + HGLGEH RY +A+ AGL D RGHG SGG + L+RD+ Sbjct: 75 AVVVLAHGLGEHARRYDHVAQRLGAAGLVTYALDHRGHGRSGGKR-------VLVRDISE 127 Query: 92 SFDDFSRRT--------ECVPVLFGHSMGGLVAARFATGGYSP--VRALALSSPALALDM 141 DF C ++ GHSMGG + FA G P + LS+PA+A Sbjct: 128 YTADFDTLVGIATREYPGCKRIVLGHSMGGGIV--FAYGVERPDNYDLMVLSAPAVA--A 183 Query: 142 KGWQKLLLAVST----AIAPGLALPTALPASRISHDPHEVRAYRKDPL-NHGKIAPRMLH 196 + ++AV+ + PGL + L + IS DP V+AY DPL +HG++ + Sbjct: 184 QDLVSPVVAVAAKLLGVVVPGLPV-QELDFTAISRDPEVVQAYNTDPLVHHGRVPAGIGR 242 Query: 197 FMLDAMRQVEREAGQFTRPVLLQVAGDDAFVAPRGSRAFFDALPSGRKTLHWYEHAYHEI 256 +L + R A T P+L+ DD + GSR + + S L Y YHE+ Sbjct: 243 ALLQVGETMPRRAPALTAPLLVLHGTDDRLIPIEGSRRLVECVGSADVQLKEYPGLYHEV 302 Query: 257 FNEEASLRAQAMQDLRNWLAQ 277 FNE R Q + D+ WL + Sbjct: 303 FNEPE--RNQVLDDVVAWLTE 321 >MT0192 [I] COG2267 Lysophospholipase MtC Mycobacterium tuberculosis CDC1551 Length = 323 Score = 107 bits (268), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 86/261 (32%), Positives = 121/261 (46%), Gaps = 29/261 (11%) Query: 32 AAVQMVHGLGEHGGRYFSLARLFNEAGLSVRICDQRGHGNSGGVQGSLIRPDDLLRDLKL 91 A V + HGLGEH RY +A+ AGL D RGHG SGG + L+RD+ Sbjct: 75 AVVVLAHGLGEHARRYDHVAQRLGAAGLVTYALDHRGHGRSGGKR-------VLVRDISE 127 Query: 92 SFDDFSRRT--------ECVPVLFGHSMGGLVAARFATGGYSP--VRALALSSPALALDM 141 DF C ++ GHSMGG + FA G P + LS+PA+A Sbjct: 128 YTADFDTLVGIATREYPGCKRIVLGHSMGGGIV--FAYGVERPDNYDLMVLSAPAVA--A 183 Query: 142 KGWQKLLLAVST----AIAPGLALPTALPASRISHDPHEVRAYRKDPL-NHGKIAPRMLH 196 + ++AV+ + PGL + L + IS DP V+AY DPL +HG++ + Sbjct: 184 QDLVSPVVAVAAKLLGVVVPGLPV-QELDFTAISRDPEVVQAYNTDPLVHHGRVPAGIGR 242 Query: 197 FMLDAMRQVEREAGQFTRPVLLQVAGDDAFVAPRGSRAFFDALPSGRKTLHWYEHAYHEI 256 +L + R A T P+L+ DD + GSR + + S L Y YHE+ Sbjct: 243 ALLQVGETMPRRAPALTAPLLVLHGTDDRLIPIEGSRRLVECVGSADVQLKEYPGLYHEV 302 Query: 257 FNEEASLRAQAMQDLRNWLAQ 277 FNE R Q + D+ WL + Sbjct: 303 FNEPE--RNQVLDDVVAWLTE 321 >DR1537 [I] COG2267 Lysophospholipase Dra Deinococcus radiodurans Length = 282 Score = 106 bits (265), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 92/272 (33%), Positives = 137/272 (50%), Gaps = 22/272 (8%) Query: 17 GTRLFCRDWLLPGATAAVQMVHGLGEHGGRYF----SLARLFNEAGLSVRICDQRGHGNS 72 GT + W AAV + HGLGE+ RY +L +AG +V DQRGHGNS Sbjct: 16 GTPVTGYVWAAESPRAAVLLTHGLGEYARRYVDHFGALIPHLVQAGYTVYAYDQRGHGNS 75 Query: 73 GGVQGSLIRPDDLLRDLKLSFDDFSRRTECVPVL-FGHSMGGLVAARFATGGYSPVRALA 131 G +G L+ LL D + + R++ +PV FGHS+GGL+ A A + + Sbjct: 76 PGERG-LVDTAPLLEDHFRAREAL--RSQPLPVYTFGHSLGGLITAASAARDPRGLSGVI 132 Query: 132 LSSPALALDMKGWQKLLLAVSTAIAPGLA-LPTALPASRISHD-----PHEVRAYRKDP- 184 LSSPAL + +G +L + A+AP LA + LP S + D EVRAY+ D Sbjct: 133 LSSPALLIG-EGQPQL----TKALAPLLARVAPRLPVSELGTDALSRRSDEVRAYQDDEN 187 Query: 185 LNHGKIAPRMLHFMLDAMRQVEREAGQFTRPVLLQVAGDDAFVAPRGSRAFFDALPSGRK 244 + HGK+ + ML ++ + ++ P L+ D +GS+ F + +P+ K Sbjct: 188 IYHGKVTAQTAWTMLRLSGELWPDYVRWQLPTLVVHGDQDQLADVKGSQRFIETIPAADK 247 Query: 245 TLHWYEHAYHEIFNEEASLRAQAMQDLRNWLA 276 TL +E YHE+ N+E S + Q + +WLA Sbjct: 248 TLRVFEGGYHELLNDEPS--DEVRQIILDWLA 277 >BS_ytpA [I] COG2267 Lysophospholipase Bsu Bacillus subtilis Length = 259 Score = 94.7 bits (234), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 71/240 (29%), Positives = 110/240 (45%), Gaps = 7/240 (2%) Query: 25 WLLPGATAAVQMVHGLGEHGGRYFSLARLFNEAGLSVRICDQRGHGNSGGVQGSLIRPDD 84 W A + ++HG E+ GRY L ++ +G V + D G G + +G + + Sbjct: 4 WKADRPVAVIVIIHGASEYHGRYKWLIEMWRSSGYHVVMGDLPGQGTTTRARGHIRSFQE 63 Query: 85 LLRDLKLSFDDFSRRTECVPV-LFGHSMGGLVAARFATGGYSP-VRALALSSPALALDMK 142 + ++ D RT +PV L GHSMGGLVA + +P + + LSSP L L +K Sbjct: 64 YIDEVDAWID--KARTFDLPVFLLGHSMGGLVAIEWVKQQRNPRITGIILSSPCLGLQIK 121 Query: 143 GWQKLLLAVS--TAIAPGLALPTALPASRISHDPHEVRAYRKDPLNHGKIAPRMLHFMLD 200 + L LA IAP L + + L + + + A + D L K++ R +L Sbjct: 122 VNKALDLASKGLNVIAPSLKVDSGLSIDMATRNEDVIEADQNDSLYVRKVSVRWYRELLK 181 Query: 201 AMRQVEREAGQFTR-PVLLQVAGDDAFVAPRGSRAFFDALPSGRKTLHWYEHAYHEIFNE 259 + F + P+L+ AGDD V +F+ + S K +E YHEIFNE Sbjct: 182 TIESAMVPTEAFLKVPLLVMQAGDDKLVDKTMVIKWFNGVASHNKAYREWEGLYHEIFNE 241 >ML2603 [I] COG2267 Lysophospholipase Mle Mycobacterium leprae Length = 279 Score = 94.0 bits (232), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 83/259 (32%), Positives = 115/259 (44%), Gaps = 25/259 (9%) Query: 32 AAVQMVHGLGEHGGRYFSLARLFNEAGLSVRICDQRGHGNSGGVQGSLIRPDDLLRDLKL 91 A + + HG GEH RY +A F AGL+ D RGHG S G + L+RDL Sbjct: 31 AVIILAHGFGEHARRYDHVAHYFAAAGLATYALDLRGHGRSAGKR-------VLVRDLSE 83 Query: 92 SFDDFSRRTECVP--------VLFGHSMGGLVAARFATGGYSP--VRALALSSPALAL-D 140 DF ++ GHSMGG + FA G P + LS PA+A D Sbjct: 84 YNADFDILVGIATRDHPGLKRIVAGHSMGGAIV--FAYGVERPDNYDLMVLSGPAVAAQD 141 Query: 141 MKGWQKLLLAVSTA-IAPGLALPTALPASRISHDPHEVRAYRKDPL-NHGKIAPRMLHFM 198 M + ++ +APGL + L IS + V AY+ DPL HGK+ + M Sbjct: 142 MVSPLRAVVGKGLGLVAPGLPV-HQLEVDAISRNRAVVAAYKDDPLVYHGKVPAGVGRVM 200 Query: 199 LDAMRQVEREAGQFTRPVLLQVAGDDAFVAPRGSRAFFDALPSGRKTLHWYEHAYHEIFN 258 L + R A T P+L+ +D V GS + + S L Y YHE+FN Sbjct: 201 LQVGETMTRRAPVLTTPLLVVHGSEDRLVLVDGSHRLVECVGSTDVELKVYPGLYHEVFN 260 Query: 259 EEASLRAQAMQDLRNWLAQ 277 E R Q ++D+ W+ + Sbjct: 261 EPE--RDQVLEDVVCWILK 277