BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0002.0099.p (354 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value yraQ [R] COG0701 Predicted permeases Eco Escherichia coli K12 411 e-114 ECs4032 [R] COG0701 Predicted permeases Ecs Escherichia coli O15... 411 e-114 ZyraQ [R] COG0701 Predicted permeases EcZ Escherichia coli O157:... 404 e-112 TM0997 [R] COG0701 Predicted permeases Tma Thermotoga maritima 79 1e-14 MTH894 [R] COG0701 Predicted permeases Mth Methanothermobacter t... 77 5e-14 MA3937 [R] COG0701 Predicted permeases Mac Methanosarcina acetiv... 74 4e-13 MJ0584 [R] COG0701 Predicted permeases Mja Methanococcus jannaschii 69 1e-11 MK1407 [R] COG0701 Predicted permeases Mka Methanopyrus kandleri... 68 3e-11 >yraQ [R] COG0701 Predicted permeases Eco Escherichia coli K12 Length = 346 Score = 411 bits (1056), Expect = e-114, Method: Compositional matrix adjust. Identities = 210/331 (63%), Positives = 250/331 (75%), Gaps = 10/331 (3%) Query: 2 SSFSSPTPTNKTSAWLKPLLFVLVAAVGLYYVKWSPYYAKSFVAADKHSIGASILANAPA 61 SS + TP W KP LF LV GL+YVKW PYY K+F AA+ HSIG SILA A A Sbjct: 5 SSSQAATPIQ----WWKPALFFLVVIAGLWYVKWEPYYGKAFTAAETHSIGKSILAQADA 60 Query: 62 APWSAALEYAEIYFLAIWKAAVLGIVLGSLVQVLIPRDWLLRMFGRAGLGATLRGGLFAL 121 PW AAL+YA IYFLA+WKAAVLG++LGSL+QVLIPRDWLLR G++ TL G LF+L Sbjct: 61 NPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLRTLGQSRFRGTLLGTLFSL 120 Query: 122 PGMMCSCCAAPVVAGMRRQQVSVGAALAFWIANPVLNPATLVFMGFVLGWHFAALRLVAG 181 PGMMC+CCAAPV AGMRRQQVS+G ALAFW+ NPVLNPATLVFMGFVLGW FAA+RLVAG Sbjct: 121 PGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPATLVFMGFVLGWGFAAIRLVAG 180 Query: 182 ILLVLGVALIAQRVAQSETLPQAAMDALAATEYEPLEDGRHFLVRWGSKLWQLFWTTAPV 241 +++VL +A + Q+ + QA + E + E F RWG LW LFW+T PV Sbjct: 181 LVMVLLIATLVQKWVRETPQTQAPV------EIDIPEAQGGFFSRWGRALWTLFWSTIPV 234 Query: 242 YIVAVLALGAARVWLFPQVDAAMGNSLLWIVALAVVGTLFVIPTAAEIPIVQSMLSLGMG 301 YI+AVL LGAARVWLFP D A+ NSL+W+VA+AV G LFVIPTAAEIPIVQ+M+ GMG Sbjct: 235 YILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVIPTAAEIPIVQTMMLAGMG 294 Query: 302 VGPAVALLMTLPSISLPSLLMLRHSFTSRVL 332 PA+ALLMTLP++SLPSL+MLR +F ++ L Sbjct: 295 TAPALALLMTLPAVSLPSLIMLRKAFPAKAL 325 >ECs4032 [R] COG0701 Predicted permeases Ecs Escherichia coli O157:H7 Length = 346 Score = 411 bits (1056), Expect = e-114, Method: Compositional matrix adjust. Identities = 210/331 (63%), Positives = 250/331 (75%), Gaps = 10/331 (3%) Query: 2 SSFSSPTPTNKTSAWLKPLLFVLVAAVGLYYVKWSPYYAKSFVAADKHSIGASILANAPA 61 SS + TP W KP LF LV GL+YVKW PYY K+F AA+ HSIG SILA A A Sbjct: 5 SSSQAATPIQ----WWKPALFFLVVIAGLWYVKWQPYYGKAFTAAETHSIGKSILAQADA 60 Query: 62 APWSAALEYAEIYFLAIWKAAVLGIVLGSLVQVLIPRDWLLRMFGRAGLGATLRGGLFAL 121 PW AAL+YA IYFLA+WKAAVLG++LGSL+QVLIPRDWLLR G++ TL G LF+L Sbjct: 61 NPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLRTLGQSRFRGTLLGTLFSL 120 Query: 122 PGMMCSCCAAPVVAGMRRQQVSVGAALAFWIANPVLNPATLVFMGFVLGWHFAALRLVAG 181 PGMMC+CCAAPV AGMRRQQVS+G ALAFW+ NPVLNPATLVFMGFVLGW FAA+RLVAG Sbjct: 121 PGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPATLVFMGFVLGWGFAAIRLVAG 180 Query: 182 ILLVLGVALIAQRVAQSETLPQAAMDALAATEYEPLEDGRHFLVRWGSKLWQLFWTTAPV 241 +++VL +A + Q+ + QA + E + E F RWG LW LFW+T PV Sbjct: 181 LVMVLLIATLVQKWVRETPQTQAPV------EIDIPEAQGGFFSRWGRALWTLFWSTIPV 234 Query: 242 YIVAVLALGAARVWLFPQVDAAMGNSLLWIVALAVVGTLFVIPTAAEIPIVQSMLSLGMG 301 YI+AVL LGAARVWLFP D A+ NSL+W+VA+AV G LFVIPTAAEIPIVQ+M+ GMG Sbjct: 235 YILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVIPTAAEIPIVQTMMLAGMG 294 Query: 302 VGPAVALLMTLPSISLPSLLMLRHSFTSRVL 332 PA+ALLMTLP++SLPSL+MLR +F ++ L Sbjct: 295 TAPALALLMTLPAVSLPSLIMLRKAFPAKAL 325 >ZyraQ [R] COG0701 Predicted permeases EcZ Escherichia coli O157:H7 EDL933 Length = 346 Score = 404 bits (1039), Expect = e-112, Method: Compositional matrix adjust. Identities = 208/331 (62%), Positives = 248/331 (74%), Gaps = 10/331 (3%) Query: 2 SSFSSPTPTNKTSAWLKPLLFVLVAAVGLYYVKWSPYYAKSFVAADKHSIGASILANAPA 61 SS + TP W KP LF LV GL+YVKW PYY K+F AA+ HSIG SILA A A Sbjct: 5 SSSQAATPIQ----WWKPALFFLVVIAGLWYVKWQPYYGKAFTAAETHSIGKSILAQADA 60 Query: 62 APWSAALEYAEIYFLAIWKAAVLGIVLGSLVQVLIPRDWLLRMFGRAGLGATLRGGLFAL 121 PW AAL+YA IYFLA+WKAAVLG++LGSL+QVLIPRDWLLR G++ TL G LF+L Sbjct: 61 NPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLRTLGQSRFRGTLLGTLFSL 120 Query: 122 PGMMCSCCAAPVVAGMRRQQVSVGAALAFWIANPVLNPATLVFMGFVLGWHFAALRLVAG 181 PGMMC+CCAAPV AGMRRQQVS+G ALAFW+ PVLNPATLV MGFVLGW FAA+RLVAG Sbjct: 121 PGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGXPVLNPATLVXMGFVLGWGFAAIRLVAG 180 Query: 182 ILLVLGVALIAQRVAQSETLPQAAMDALAATEYEPLEDGRHFLVRWGSKLWQLFWTTAPV 241 +++VL +A + Q+ + QA + E + E F RWG LW LFW+T PV Sbjct: 181 LVMVLLIATLVQKWVRETPQTQAPV------EIDIPEAQGGFFSRWGRALWTLFWSTIPV 234 Query: 242 YIVAVLALGAARVWLFPQVDAAMGNSLLWIVALAVVGTLFVIPTAAEIPIVQSMLSLGMG 301 YI+AVL LGAARVWLFP D A+ NSL+W+VA+AV G LFVIPTAAEIPIVQ+M+ GMG Sbjct: 235 YILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVIPTAAEIPIVQTMMLAGMG 294 Query: 302 VGPAVALLMTLPSISLPSLLMLRHSFTSRVL 332 PA+ALLMTLP++SLPSL+MLR +F ++ L Sbjct: 295 TAPALALLMTLPAVSLPSLIMLRKAFPAKAL 325 >TM0997 [R] COG0701 Predicted permeases Tma Thermotoga maritima Length = 365 Score = 79.3 bits (194), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 81/308 (26%), Positives = 126/308 (40%), Gaps = 60/308 (19%) Query: 92 VQVLIPRDWLLRMFGRAGLG------ATLRGGLFALPGMMCSCCAAPVVAGMRRQQVSVG 145 + V++ +D +L++ G A + GG+ A +CSC P+ G+ ++ +G Sbjct: 60 ISVMLKKDAVLKLLGPNAKRIISYPVAAISGGILA----VCSCTILPLFGGIYKKGAGIG 115 Query: 146 AALAFWIANPVLNPATLVFMGFVLGWHFAALRLVAGILLVLGVALIAQRVAQ-------- 197 A F A P +N A + VLGW RL+A I + + LI + + Q Sbjct: 116 PATTFLFAGPAINIAAIFLTARVLGWDLGLARLIATITAAVLIGLIMEMIYQERGEGGLA 175 Query: 198 ------------------------------SETLPQAAMDALAATEYEPLEDG--RHFLV 225 ++TL + M L + G R + Sbjct: 176 FTSDDDQYGVRGIIFFLIQLGFLVTSSLGINQTLKYSLMTLLGISALFMALFGFKRDTVE 235 Query: 226 RWGSKLWQLFWTTAPVYIVAVLALGAARVWLFPQVDAAM--GNSLLWIVALAVVGTLFVI 283 W + W P + V G L QV A+ NS L + +V+GTL Sbjct: 236 NWLYETWDFAKKILPYLFIGVFFAGVLTRLLPQQVVTALLGSNSFLSNLVASVIGTLMYF 295 Query: 284 PTAAEIPIVQSMLSLGMGVGPAVALLMTLPSISLPSLLML------RHSFTSRVLTTV-- 335 T E+PIVQ++ LGM GP +ALLM S+SLPS++++ + +FT L V Sbjct: 296 ATLTEVPIVQALRELGMAKGPTLALLMAGNSLSLPSMIVITKLLGKKKAFTYFGLVVVFS 355 Query: 336 TLLTMLIG 343 TL M+ G Sbjct: 356 TLFGMIYG 363 >MTH894 [R] COG0701 Predicted permeases Mth Methanothermobacter thermautotrophicus Length = 327 Score = 77.0 bits (188), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 59/221 (26%), Positives = 110/221 (49%), Gaps = 15/221 (6%) Query: 126 CSCCAAPVVAGMRRQQVSVGAALAFWIANPVLNPATLVFMGFVLGWHFAALRLVAG-ILL 184 CSC A P+ G V +GA +F I++P++N ++ + + GW A +++G I+ Sbjct: 90 CSCSAVPLFIGFVEAGVPLGATFSFLISSPMINEIAIILLLGLFGWQITAFYILSGFIIA 149 Query: 185 VLGVALIAQRVAQS-------ETLPQAAMDALAATEYE-PLEDGRHFLVRWGSKLWQLFW 236 VLG LI + ++ ETL + M AL + E P R V +++ + Sbjct: 150 VLGGILIGKLKMETELEDYVYETLEK--MRALGVADVELPKPTLRERYVIAKNEMKDILR 207 Query: 237 TTAPVYIVAVLALGAARVWLFPQ---VDAAMGNSLLWIVALAVVGTLFVIPTAAEIPIVQ 293 +P YIV +A+G P+ + A +++ + ++G A IP++ Sbjct: 208 RVSP-YIVIAIAIGGWIHGYLPEDFLLQYAGADNIFAVPMAVIIGVPLYSNAAGTIPLIS 266 Query: 294 SMLSLGMGVGPAVALLMTLPSISLPSLLMLRHSFTSRVLTT 334 +++ GM G A+AL+M++ ++SLP +++LR ++L T Sbjct: 267 ALIEKGMAAGTALALMMSITALSLPEMIILRKVMKPKLLAT 307 >MA3937 [R] COG0701 Predicted permeases Mac Methanosarcina acetivorans str.C2A Length = 350 Score = 74.3 bits (181), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 66/269 (24%), Positives = 112/269 (41%), Gaps = 46/269 (17%) Query: 125 MCSCCAAPVVAGMRRQQVSVGAALAFWIANPVLNPATLVFMGFVLGWHFAALRLVAGILL 184 +CSC P+ AG+ ++ +G A F + P +N +V+ +LG+ A R A + L Sbjct: 74 VCSCTVLPLFAGIYKRGAGIGPATTFLFSAPAINILAIVYTAKILGYDLGAARAFAAVTL 133 Query: 185 VLGVALIAQRVAQSETLPQAAMDALAATEYE----------------------------- 215 + V +I + + + + E++ Sbjct: 134 SVLVGIIMSLAYERKDTERKPIKTFGEAEHKHSVQLFVLLIVVLIAPELMSSWGWKYTFQ 193 Query: 216 -----PLEDGRHFL-VRWGSK---------LWQLFWTTAPVYIVAVLALGAARVWLFPQV 260 PL +L RW SK W L P+ ++ V G A V L + Sbjct: 194 ILAWAPLIGLTAYLSYRWFSKEEINSWMGETWFLIKQITPLLLLGVFFAGIAVVVLPKEY 253 Query: 261 DAAM--GNSLLWIVALAVVGTLFVIPTAAEIPIVQSMLSLGMGVGPAVALLMTLPSISLP 318 AA GNSL ++ G L T E+PI++++ LGMG GP++A+L+ P++SLP Sbjct: 254 VAAFVGGNSLTSNFISSIAGALMYFSTLTEVPIIKALTLLGMGAGPSLAMLLAGPALSLP 313 Query: 319 SLLMLRHSFTSRVLTTVTLLTMLIGAASG 347 +++++ + T L +LI SG Sbjct: 314 NMIVINRIMGVKKGMTYICLVVLIATFSG 342 >MJ0584 [R] COG0701 Predicted permeases Mja Methanococcus jannaschii Length = 365 Score = 68.9 bits (167), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 78/311 (25%), Positives = 131/311 (42%), Gaps = 73/311 (23%) Query: 95 LIPRDWLLRMFGR------AGLGATLRGGLFALPGMMCSCCAAPVVAGMRRQQVSVGAAL 148 +I ++++++ FG + A + G L A+ CSC P+ A + ++ +G A Sbjct: 45 MINKNFIIKYFGSNTPKYISYTVAAVSGSLLAV----CSCTILPLFASIYKRGAGIGPAT 100 Query: 149 AFWIANPVLNPATLVFMGFVLGWHFAALR----LVAGILLVLGVALI--------AQRVA 196 F + P +N + + +LGW LR +V IL+ L + +I A RV Sbjct: 101 TFLFSGPAINVLAIFYSAALLGWDIGFLRAVFAVVVSILIGLSMEIIFKSHEKKRALRVP 160 Query: 197 QSETLPQ--------------------AAMDALAATEYEPLEDG---RHF---------- 223 +++ + A L T PL DG +H Sbjct: 161 KADKISDRPLYQTITFFALQFIMLLVITASPKLFPTLSMPLYDGFLLKHLLFIILGIILA 220 Query: 224 -----------LVRWGSKLWQLFWTTAPVYIVAVLALGAARVWLFPQVDAAM--GNSLLW 270 + W + + L P+ I+ V GA + + P A GNS+ Sbjct: 221 VTTKIWFKDEEIKNWLRESFTLLKIVFPLLIIGVAIAGAIKAIIPPSYIATYVGGNSITA 280 Query: 271 IVALAVVGTLFVIPTAAEIPIVQSMLSLGMGVGPAVALLMTLPSISLPSLLMLRHSFTSR 330 + +G L T E+PI+++++ LGMGVGPA+ALL+ PS+S+P++L + S+ Sbjct: 281 NFIASFIGALMYFATLTEVPIIKALMELGMGVGPAMALLLAGPSLSIPTVLTI-----SK 335 Query: 331 VLTTVTLLTML 341 VL LT L Sbjct: 336 VLGKTKALTYL 346 >MK1407 [R] COG0701 Predicted permeases Mka Methanopyrus kandleri AV19 Length = 333 Score = 67.8 bits (164), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 65/273 (23%), Positives = 124/273 (45%), Gaps = 15/273 (5%) Query: 83 VLGIVLGSLVQVLIPRDWLLRMFGRAGLGATLRGGLFALPGMMCSCCAAPVVAGMRRQQV 142 ++G+VLG+ +Q ++P +L R R GL A + L +P +CSC P+ + + Sbjct: 25 LIGLVLGAALQSMVPERYLARHL-RGGLKACIIATLLGVPLPLCSCSVLPLAIAAKNRGA 83 Query: 143 SVGAALAFWIANPVLNPATLVFMGFVLGWHFAALRLVAGILLVLGVALIAQ-----RVAQ 197 VG +AF +A P + +L+ +LG +F A +++ ++ + V +A+ R + Sbjct: 84 GVGPVIAFIVATPQTDVNSLLLAYALLGPYFTAAKVLTAVVAAVIVGYLAELTLKDRSEE 143 Query: 198 SETLPQAAMDALAATEYEPLEDGRHFLVRWGSKLWQLFWTTAPVYIVAVLALGAARVWLF 257 +E LP E+ P GR F + +L +L T P ++ + A +W+ Sbjct: 144 TEELPSCG-------EHGPCYRGRSF-KDFPRELLELSGTVGPWLLIGLAASVLLELWV- 194 Query: 258 PQVDAAMGNSLLWIVALAVVGTLFVIPTAAEIPIVQSMLSLGMGVGPAVALLMTLPSISL 317 P L V A++ + + A IPI ++++ G VG + + P ++ Sbjct: 195 PSPAVRYLEGFLGPVLAALISLPLYVCSVAAIPIAAALVAKGASVGSMLTFTVAGPGTNV 254 Query: 318 PSLLMLRHSFTSRVLTTVTLLTMLIGAASGLAA 350 +L ++ R++ + L A+G AA Sbjct: 255 ATLSVILRFLGGRIVAAYLVGITLCAVAAGYAA 287