BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0065.p (519 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0618_1 [H] COG1010 Precorrin-3B methylase Rso Ralstonia solan... 342 1e-93 RSp0618_2 [H] COG2099 Precorrin-6x reductase Rso Ralstonia solan... 322 9e-88 TVN0921 [H] COG1010 Precorrin-3B methylase Tvo Thermoplasma volc... 189 2e-47 lin1162 [H] COG1010 Precorrin-3B methylase Lin Listeria innocua 179 2e-44 STM2027 [H] COG1010 Precorrin-3B methylase Sty Salmonella typhim... 172 2e-42 slr0969_2 [H] COG1010 Precorrin-3B methylase Syn Synechocystis 171 3e-42 AF0724_1 [H] COG1010 Precorrin-3B methylase Afu Archaeoglobus fu... 168 3e-41 CAC1382 [H] COG1010 Precorrin-3B methylase Cac Clostridium aceto... 165 2e-40 FN0951 [H] COG1010 Precorrin-3B methylase Fnu Fusobacterium nucl... 161 4e-39 lin1163 [H] COG2099 Precorrin-6x reductase Lin Listeria innocua 159 1e-38 >RSp0618_1 [H] COG1010 Precorrin-3B methylase Rso Ralstonia solanacearum Length = 243 Score = 342 bits (877), Expect = 1e-93, Method: Compositional matrix adjust. Identities = 172/242 (71%), Positives = 192/242 (79%) Query: 7 KGVLNLVSVGPGFEEMIIPRAIEALRESEVIVAYELYLRWIAAHIEGKEIHTPPLTQERE 66 G L LVSVGPG E+I P AL ++VIVAY+LYL WIA I K+I T PLTQER Sbjct: 2 SGKLYLVSVGPGTRELIPPMVKTALDAADVIVAYDLYLTWIAPWITAKDIRTLPLTQERG 61 Query: 67 RALLAIEQARAGRRVALISSGDIGIYAMAALAFDDMREDDTYEVNVIPGVTSANACASLL 126 RA LAI +ARAGR VALISSGDIGIYAMAALAF+ M E DT++V VIPG+TSANACASLL Sbjct: 62 RAELAIAEARAGRTVALISSGDIGIYAMAALAFEAMAEHDTFDVQVIPGITSANACASLL 121 Query: 127 GSPLSHDFATLSLSNLLCPWTWIEQRASHIAQADLACVLYNVQSAGRQEGVYRILQLMLQ 186 GSPLSHDFATLSLS+LLCPW WIE RA HIAQADLA VLYNVQS RQEGVYRIL ++L+ Sbjct: 122 GSPLSHDFATLSLSDLLCPWAWIETRARHIAQADLAVVLYNVQSRQRQEGVYRILDILLE 181 Query: 187 HKAPHTLCGVVRNAYRPGQKVSIHRLDELPGLQFDMLTSIVIGNRFTQRKRDWIFTPRGY 246 HK TLCG+V NAYR GQ I L EL +FDMLTS+VIGNRFT+RKR WI+TPRGY Sbjct: 182 HKRAETLCGIVHNAYRDGQAAEIVTLAELRTRRFDMLTSLVIGNRFTRRKRGWIYTPRGY 241 Query: 247 ND 248 + Sbjct: 242 GN 243 >RSp0618_2 [H] COG2099 Precorrin-6x reductase Rso Ralstonia solanacearum Length = 276 Score = 322 bits (826), Expect = 9e-88, Method: Compositional matrix adjust. Identities = 168/268 (62%), Positives = 195/268 (72%) Query: 249 WSEESQAEEVAQLPSQAVWVFAGTSDGNALAAELAGQGRPVVVSTATGYGGELARSDCPG 308 W + A LP+ AVWVF+GTSDGNALA ++A GR VVVS+A+ YG LA +CPG Sbjct: 1 WDDTPDTVTAAGLPAHAVWVFSGTSDGNALARDIAATGRAVVVSSASDYGAALAGQNCPG 60 Query: 309 AFVWSGRQGLEARRQALRDSRARVLVDATHPFAVQMSQQLMTLSQELGIPYLRYERPGEL 368 V +GR G+E RR+ LR+S A +VDATHP+A +MSQQL+ L+QEL +PYLRYERPG+ Sbjct: 61 VAVVAGRLGIERRRELLRESAAHAIVDATHPYAAEMSQQLIRLAQELALPYLRYERPGDA 120 Query: 369 AADDPHACADMEDAAHKAMAMGQRIFLATGSKDLATFLQAPGAQERQWFARVTADPDFIS 428 A A MEDAA AM G+RIFLATGSKDLATF+ A A WF RV DP + Sbjct: 121 QAAPAQRAASMEDAARLAMQHGRRIFLATGSKDLATFVNADAAGRHAWFVRVAPDPAHLQ 180 Query: 429 RALELGLPRAHLCAMQGPFSQAFNEALWRDWGIDCVVTKDSGEAGGYHAKAEAARALGIP 488 RA++ G+PRA L AMQGPFSQA NEALWRDWGIDCVVTKDSGEAGGYHAKA AA ALGIP Sbjct: 181 RAIDAGIPRARLVAMQGPFSQAANEALWRDWGIDCVVTKDSGEAGGYHAKAAAAAALGIP 240 Query: 489 LLTVRRPPLDYPQVAHDFGSVHAHLAAL 516 L+ V RP +DYP VAHDF V LA L Sbjct: 241 LIVVDRPAIDYPAVAHDFAGVLMQLADL 268 >TVN0921 [H] COG1010 Precorrin-3B methylase Tvo Thermoplasma volcanium Length = 251 Score = 189 bits (479), Expect = 2e-47, Method: Compositional matrix adjust. Identities = 97/240 (40%), Positives = 149/240 (62%), Gaps = 2/240 (0%) Query: 10 LNLVSVGPGFEEMIIPRAIEALRESEVIVAYELYLRWIAAHIEGKEIHTPPLTQERERAL 69 L +V +GPG +++ P+A A+ SE ++ + LY+ +I K + + E ERA Sbjct: 5 LFVVGIGPGSNDLMTPQASLAIERSEYVIGFSLYVSFIKEKFPNKIYISNGMQGEIERAK 64 Query: 70 LAIEQARAGRRVALISSGDIGIYAMAALAFDDMR--EDDTYEVNVIPGVTSANACASLLG 127 AIE AR+G V++ISSGD GIY +A F+ + ED+ EV+V+PG+++ ++CASLLG Sbjct: 65 KAIELARSGHNVSIISSGDAGIYGIAGAVFEILSTLEDNMPEVSVVPGISALSSCASLLG 124 Query: 128 SPLSHDFATLSLSNLLCPWTWIEQRASHIAQADLACVLYNVQSAGRQEGVYRILQLMLQH 187 SPLS+DF LSLS+LL PW I +RA A DL V+YN + + + ++++ L++ Sbjct: 125 SPLSNDFMVLSLSDLLTPWEVIRKRAEAAAILDLVTVIYNPRGSRFPNNLEKVMEYFLKY 184 Query: 188 KAPHTLCGVVRNAYRPGQKVSIHRLDELPGLQFDMLTSIVIGNRFTQRKRDWIFTPRGYN 247 ++ T G+VRNAYRP Q I +L + + DMLT+++IGN T W+ T RGY+ Sbjct: 185 RSGDTPVGIVRNAYRPDQSAKIVQLKDFSCSEIDMLTTVIIGNSETYVSGRWMATRRGYS 244 >lin1162 [H] COG1010 Precorrin-3B methylase Lin Listeria innocua Length = 241 Score = 179 bits (453), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 86/240 (35%), Positives = 147/240 (61%), Gaps = 1/240 (0%) Query: 9 VLNLVSVGPGFEEMIIPRAIEALRESEVIVAYELYLRWIAAHIEGKEIHTPPLTQERERA 68 ++ ++ +GPG + ++ A+EA+ +++VIV Y Y++ I I+ KE+ + +E +R Sbjct: 1 MIYVIGIGPGDKRLMTGEALEAIADADVIVGYVTYIKLIKELIKDKEVVKTGMRREIDRC 60 Query: 69 LLAIEQARAGRRVALISSGDIGIYAMAALAFD-DMREDDTYEVNVIPGVTSANACASLLG 127 A+ A G++VA++SSGD GIY MA L + + + EV VIPG+T++ A++LG Sbjct: 61 QEAVNIALTGKKVAVVSSGDAGIYGMAGLVLELAEKSNPELEVKVIPGITASIGAAAVLG 120 Query: 128 SPLSHDFATLSLSNLLCPWTWIEQRASHIAQADLACVLYNVQSAGRQEGVYRILQLMLQH 187 +P+ HDF +SLS+L+ PW IE+R +H A AD YN +S GR + Q M++H Sbjct: 121 APIMHDFCHISLSDLMTPWEVIEKRLTHAAMADFVVCFYNPRSKGRANHLANAFQKMMEH 180 Query: 188 KAPHTLCGVVRNAYRPGQKVSIHRLDELPGLQFDMLTSIVIGNRFTQRKRDWIFTPRGYN 247 K+P T+ G+V++ R ++ I ++++ DM T +++GN+ T K + TPRGY+ Sbjct: 181 KSPKTVVGIVKDVGRKEERKIITTMEDIDYELVDMTTMVIVGNKETYVKNGKMITPRGYS 240 >STM2027 [H] COG1010 Precorrin-3B methylase Sty Salmonella typhimurium LT2 Length = 241 Score = 172 bits (435), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 93/240 (38%), Positives = 143/240 (59%), Gaps = 1/240 (0%) Query: 9 VLNLVSVGPGFEEMIIPRAIEALRESEVIVAYELYLRWIAAHIEGKEIHTPPLTQERERA 68 +L+++ +GPG + M+ AIEAL+ +E++V Y+ Y + A K++ + +E ER Sbjct: 1 MLSVIGIGPGSQAMMTMEAIEALQAAEIVVGYKTYTHLVKAFTGDKQVIKTGMCREIERC 60 Query: 69 LLAIEQARAGRRVALISSGDIGIYAMAALAFDDM-REDDTYEVNVIPGVTSANACASLLG 127 AIE A+AG VALISSGD GIY MA L + + ++ EV +IPG+T++ A ASLLG Sbjct: 61 QAAIELAQAGHNVALISSGDAGIYGMAGLVLELVSKQKLDVEVRLIPGMTASIAAASLLG 120 Query: 128 SPLSHDFATLSLSNLLCPWTWIEQRASHIAQADLACVLYNVQSAGRQEGVYRILQLMLQH 187 +PL HDF +SLS+LL PW IE+R +AD YN +S GR+ + R L+ Sbjct: 121 APLMHDFCHISLSDLLTPWPVIEKRIVAAGEADFVICFYNPRSRGREGHLARAFDLLAAS 180 Query: 188 KAPHTLCGVVRNAYRPGQKVSIHRLDELPGLQFDMLTSIVIGNRFTQRKRDWIFTPRGYN 247 K+ T GVV++A R ++ + L ++ DM + +++GN+ T + + TPRGY Sbjct: 181 KSAQTPVGVVKSAGRKKEEKWLTTLGDMDFEPVDMTSLVIVGNKTTYVQDGLMITPRGYT 240 >slr0969_2 [H] COG1010 Precorrin-3B methylase Syn Synechocystis Length = 257 Score = 171 bits (434), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 102/250 (40%), Positives = 142/250 (56%), Gaps = 5/250 (2%) Query: 2 EAVKKKGVLNLVSVGPGFEEMIIPRAIEALRESEVIVAYELYLRWI-AAHIEGKEIHTPP 60 E + K G L LV GPG I P A A+RE++V++ Y LYL I G+ T P Sbjct: 4 EYIGKPGQLWLVGTGPGELSQISPAARTAIREADVLIGYGLYLDLIDPLRRPGQICETFP 63 Query: 61 LTQERERALLAIEQARAGRRVALISSGDIGIYAMAALAFDDMR----EDDTYEVNVIPGV 116 LTQER+R AIE A+ G VA++SSGD GIY MA L +++ + T V V PG+ Sbjct: 64 LTQERQRCQRAIELAQWGLTVAVLSSGDCGIYGMAGLVLEELEKLNWDGQTPAVEVFPGI 123 Query: 117 TSANACASLLGSPLSHDFATLSLSNLLCPWTWIEQRASHIAQADLACVLYNVQSAGRQEG 176 ++ A A+ +G+PL HDF +SLS+LL P I QR + AQAD LYN +S R E Sbjct: 124 SAFQAAAARVGAPLMHDFCAISLSDLLTPKEKILQRLTAAAQADFVTALYNPKSQKRTEL 183 Query: 177 VYRILQLMLQHKAPHTLCGVVRNAYRPGQKVSIHRLDELPGLQFDMLTSIVIGNRFTQRK 236 + + + LQ ++P T +VR+ YR + + L + DMLT ++IGN T Sbjct: 184 IEQAQAIFLQWRSPTTPVALVRSVYRQDETIVRTTLAAMLNFPIDMLTVVLIGNSNTYNY 243 Query: 237 RDWIFTPRGY 246 +D++ TPRGY Sbjct: 244 QDYLITPRGY 253 >AF0724_1 [H] COG1010 Precorrin-3B methylase Afu Archaeoglobus fulgidus Length = 250 Score = 168 bits (425), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 95/243 (39%), Positives = 148/243 (60%), Gaps = 5/243 (2%) Query: 7 KGVLNLVSVGPGFEEMIIPRAIEALRESEVIVAYELYLRWIAAHIEGKEIHTPPLTQERE 66 +G L +V +GPG EE++ +A A+ E++ IV Y+ Y+ I+ IEGK++ T P+ +E + Sbjct: 7 RGKLYVVGIGPGKEELMTLKAKRAIEEADYIVGYQTYVDRISHLIEGKKVVTTPMRKELD 66 Query: 67 RALLAIEQARAGRRVALISSGDIGIYAMAALAFDDMREDDT-YEVNVIPGVTSANACASL 125 R +A+E A+ VALIS GD IY + L + E E+ IPGVT+A+A +SL Sbjct: 67 RVKIALELAKE-HVVALISGGDPSIYGILPLVIEYAVEKKVDVEIEAIPGVTAASAASSL 125 Query: 126 LGSPLSHDFATLSLSNLLCPWTWIEQRASHIAQADLACVLYNVQSAGRQEGVYRILQLML 185 LGS +S DFA +SLS+LL PW+ +E+R + D +YN S R+E + ++++ Sbjct: 126 LGSAISGDFAVVSLSDLLVPWSVVEKRLLYALSGDFVVAIYNPSSRRRKENFRKAMEIVR 185 Query: 186 QHKAPHTLCGVVRNAYRPGQKVSIHRLDELPGLQFDMLTSIVIGNRFTQRKRDWIFTPRG 245 + + GVVRNA R GQ+V I R+ E+ + DM T +++GN T+ +FTPRG Sbjct: 186 RFRG-DAWVGVVRNAGREGQQVEIRRVSEVD--EVDMNTILIVGNSETKVVDGKMFTPRG 242 Query: 246 YND 248 Y++ Sbjct: 243 YSN 245 >CAC1382 [H] COG1010 Precorrin-3B methylase Cac Clostridium acetobutylicum Length = 238 Score = 165 bits (418), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 88/240 (36%), Positives = 147/240 (61%), Gaps = 4/240 (1%) Query: 8 GVLNLVSVGPGFEEMIIPRAIEALRESEVIVAYELYLRWIAAHIEGKEIHTPPLTQERER 67 G L +V +GPG + + +A AL ESEVIV Y Y++ + ++GKE+ + +E +R Sbjct: 2 GKLYVVGIGPGGLQDMTLKARRALEESEVIVGYTKYIKMVEELLKGKEVFETGMKKEVDR 61 Query: 68 ALLAIEQARAGRRVALISSGDIGIYAMAALAFDDMREDDTYEVNVIPGVTSANACASLLG 127 A+E ++ + V++IS+GD GIY MA L + M++++ E+ IPGVTS+ A A+LLG Sbjct: 62 CKRALELSK-DKVVSIISTGDSGIYGMAGLILE-MKKEEIVEI--IPGVTSSIAAAALLG 117 Query: 128 SPLSHDFATLSLSNLLCPWTWIEQRASHIAQADLACVLYNVQSAGRQEGVYRILQLMLQH 187 SPL HD ++SLS+L+ P+ I++R A+ D YN +S GR + + ++ ++ Sbjct: 118 SPLMHDNCSISLSDLMTPYDLIKKRVLLAAEGDFVISFYNPRSKGRPYYLRECINIIRKY 177 Query: 188 KAPHTLCGVVRNAYRPGQKVSIHRLDELPGLQFDMLTSIVIGNRFTQRKRDWIFTPRGYN 247 + +T G+V+NA R G+++ I L + + DM++ ++IGN T K + + TPRGY Sbjct: 178 RKNNTPVGIVKNAMREGEEIIISTLKDFDETKVDMMSMVIIGNSNTFMKDNIMITPRGYK 237 >FN0951 [H] COG1010 Precorrin-3B methylase Fnu Fusobacterium nucleatum Length = 249 Score = 161 bits (407), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 90/246 (36%), Positives = 134/246 (54%), Gaps = 1/246 (0%) Query: 6 KKGVLNLVSVGPGFEEMIIPRAIEALRESEVIVAYELYLRWIAAHIEGKEIHTPPLTQER 65 G + +V +GPG E I RA L+ VI Y Y+ + KE + +E Sbjct: 2 NNGKIYVVGIGPGNMEDISIRAYNILKNVNVIAGYTTYVDLVRDEFSDKEFLVSGMKREI 61 Query: 66 ERALLAIEQARAGRRVALISSGDIGIYAMAALAFDDMREDDTYEVNVIPGVTSANACASL 125 ER +E A+ G+ VALISSGD GIY MA + + + EV V+PG+TS A A+L Sbjct: 62 ERCKEVLEVAKIGKNVALISSGDAGIYGMAGIMLE-VAMGSGIEVQVVPGITSTIAGAAL 120 Query: 126 LGSPLSHDFATLSLSNLLCPWTWIEQRASHIAQADLACVLYNVQSAGRQEGVYRILQLML 185 +G+PL HD A +SLS+LL W I++R +Q D A LYN +S GR E + ++ML Sbjct: 121 VGAPLMHDQAIISLSDLLTEWEVIKKRIECASQGDFAISLYNPKSKGRTEQIVEAREIML 180 Query: 186 QHKAPHTLCGVVRNAYRPGQKVSIHRLDELPGLQFDMLTSIVIGNRFTQRKRDWIFTPRG 245 ++K P T ++R+ R + ++ L++ + DM T +++GN T K + TPRG Sbjct: 181 KYKLPTTPVALLRHIGRKEENYTLTTLEDFLNYEIDMFTIVLVGNSNTYVKDGKMITPRG 240 Query: 246 YNDWSE 251 Y S+ Sbjct: 241 YEKKSK 246 >lin1163 [H] COG2099 Precorrin-6x reductase Lin Listeria innocua Length = 250 Score = 159 bits (402), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 87/235 (37%), Positives = 129/235 (54%), Gaps = 5/235 (2%) Query: 266 VWVFAGTSDGNALAAELAGQGRPVVVSTATGYGGELARSDCPGAFVWSGRQGLEARRQAL 325 ++V GTSD ++ L RP ++S AT YG LA+ F GR EA Sbjct: 2 IFVLGGTSDSLLISDWLTETKRPFILSVATDYGETLAKQHAEKVF--CGRLAKEAMLAKW 59 Query: 326 RDSRARVLVDATHPFAVQMSQQLMTLSQELGIPYLRYERPGELAADDPHACADMEDAAHK 385 + + ++++DATHPFA +S+ M +E GIPY+R+ER E D+ + D+ +A Sbjct: 60 QLEQIKLVIDATHPFATIVSETAMEACKEAGIPYIRFERTSE-QTDNTYLVDDINEACKV 118 Query: 386 AMAMGQRIFLATGSKDLATFLQAPGAQERQWFARVTADPDFISRALELGLPRAHLCAMQG 445 A +GQRIFL TGSK+L F+ G +++ AR+ D I A ELGL + M+G Sbjct: 119 AAKLGQRIFLTTGSKNLPEFV--AGLKQQHIIARILPVSDVIRSAEELGLVADQIIGMKG 176 Query: 446 PFSQAFNEALWRDWGIDCVVTKDSGEAGGYHAKAEAARALGIPLLTVRRPPLDYP 500 PF++ N D ++TK+SG+ GG+ K AA L IP++ +RR L+YP Sbjct: 177 PFTKEANRTQLEMTQADVLITKESGKQGGFQEKLTAAAELNIPVIVIRRKKLNYP 231