BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0064.p (267 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0619 [H] COG2073 Cobalamin biosynthesis protein CbiG Rso Rals... 307 1e-83 AF0725 [H] COG2073 Cobalamin biosynthesis protein CbiG Afu Archa... 87 4e-17 lin1161 [H] COG2073 Cobalamin biosynthesis protein CbiG Lin List... 86 7e-17 STM2028 [H] COG2073 Cobalamin biosynthesis protein CbiG Sty Salm... 85 1e-16 FN0952 [H] COG2073 Cobalamin biosynthesis protein CbiG Fnu Fusob... 77 3e-14 CAC1370 [H] COG2073 Cobalamin biosynthesis protein CbiG Cac Clos... 74 4e-13 MTH1408 [H] COG2073 Cobalamin biosynthesis protein CbiG Mth Meth... 71 3e-12 slr0969_1 [H] COG2073 Cobalamin biosynthesis protein CbiG Syn Sy... 62 2e-09 >RSp0619 [H] COG2073 Cobalamin biosynthesis protein CbiG Rso Ralstonia solanacearum Length = 254 Score = 307 bits (787), Expect = 1e-83, Method: Compositional matrix adjust. Identities = 160/254 (62%), Positives = 192/254 (75%), Gaps = 5/254 (1%) Query: 12 LWPVRAEAAALAMLLQDSLGGELYQPWLNDQGGLSQRQQFALAYRRHRQWIMIGATGIAV 71 +W VRA+A LA L +L E+Y+PW S R+ F A+ HR+WIM+ A GIAV Sbjct: 6 IWLVRADAQPLARRLARALDAEVYEPWEIPHA--SPRELFRHAFAHHRRWIMVAAAGIAV 63 Query: 72 RFLDGLPQDKHSDPAVVVVDEAGRFAIALLAGHEGGANSLAYRVAQCTGAQPVVTTATEA 131 R+LDGLP++K +DPAVVV+DEA RFAI LL GHEGGAN+LAY VA+ TG P VTTATEA Sbjct: 64 RYLDGLPRNKLTDPAVVVLDEAARFAIPLLGGHEGGANALAYEVARLTGGTPAVTTATEA 123 Query: 132 VKPLVLGIGCRKLASAEQIGRAVAQALAQCPGATLAQVREVATIDLKAEEPGLLAFCATH 191 KPL LGIGCR+ S E IGRAV AL+Q LA+VRE+ATIDLKA+EPGLLAFCA H Sbjct: 124 RKPLTLGIGCRRGKSMEAIGRAVMAALSQ---RALAEVREIATIDLKADEPGLLAFCARH 180 Query: 192 GLPLRVIARAQIAQRAWVGKPSDWVRQNVGVEGVCEPAALIASPRGRLLLGKMALDGVTV 251 GLPLRVIA+A IA R W PS WVR++VGV+GVCEP ALIASPRG+L++ K ALDGVTV Sbjct: 181 GLPLRVIAQADIAARGWTDAPSAWVRKSVGVDGVCEPCALIASPRGQLIVPKTALDGVTV 240 Query: 252 AVVDDAEGWRNVVE 265 AVV+D W++ + Sbjct: 241 AVVEDNPAWKDTTQ 254 >AF0725 [H] COG2073 Cobalamin biosynthesis protein CbiG Afu Archaeoglobus fulgidus Length = 249 Score = 87.0 bits (214), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 64/188 (34%), Positives = 107/188 (56%), Gaps = 8/188 (4%) Query: 66 ATGIAVRFLDGLPQDKHSDPAVVVVDEAGRFAIALLAGHEGGANSLAYRVAQCTGAQPVV 125 A+GI VR + G + K DPAV+V+D+ + A+ +L GH GG N +A ++ + G + V+ Sbjct: 68 ASGIVVRGICGRLRGKWKDPAVIVLDKPLKHAVVMLGGHHGG-NEVAKKLEEA-GLKAVI 125 Query: 126 TTATEAVKPLVLGIGCRKLASAEQIGRAVAQALAQCPGATLAQVREVATIDLKAEEPGLL 185 TTA E + +G+G RK A+A++I A+A AL + G + ++R ++T+D K + ++ Sbjct: 126 TTAMEFTEGYSVGVGFRKNATADEILEAIAMALEEV-GGRMEEIRVISTVDAK-RDSAIV 183 Query: 186 AFCATHGLPLRVIARAQIAQRAWVGKPSDWVRQNVGVEGVCEPAALIASPRGRLLLGKMA 245 PL + RA+ + + + V+ +GV+ V E A+ AS G L+L K Sbjct: 184 EVANRLKRPL-IFVRAEEINSMDI-RETKAVK--IGVKNVAEACAIYASNSGELILPKRV 239 Query: 246 LDGVTVAV 253 GVTVA+ Sbjct: 240 YGGVTVAI 247 >lin1161 [H] COG2073 Cobalamin biosynthesis protein CbiG Lin Listeria innocua Length = 343 Score = 86.3 bits (212), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 53/118 (44%), Positives = 71/118 (60%), Gaps = 13/118 (11%) Query: 22 LAMLLQDSLGGELYQP--WLNDQG-------GLSQRQQFALAYRRHRQWIMIGATGIAVR 72 LA+ L +L G ++ P N+Q G + R+ F H+ I I ATGIAVR Sbjct: 18 LAISLTKTLPGTVFVPEKHQNEQTAVLTPNFGTAMREMFT----THQALICIMATGIAVR 73 Query: 73 FLDGLPQDKHSDPAVVVVDEAGRFAIALLAGHEGGANSLAYRVAQCTGAQPVVTTATE 130 L + QDK SDPAV+V+DE G+F I+LL+GH GGAN L ++A TG V+TTAT+ Sbjct: 74 TLAPVIQDKLSDPAVIVMDEYGQFIISLLSGHVGGANELTEQIAAITGGAAVITTATD 131 >STM2028 [H] COG2073 Cobalamin biosynthesis protein CbiG Sty Salmonella typhimurium LT2 Length = 351 Score = 85.1 bits (209), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 44/77 (57%), Positives = 54/77 (70%) Query: 54 AYRRHRQWIMIGATGIAVRFLDGLPQDKHSDPAVVVVDEAGRFAIALLAGHEGGANSLAY 113 A+ + I IGATGIAVR L L DK SDPAVVV+DE G+ I+LL+GH GGAN+L Sbjct: 58 AFTTYTALIFIGATGIAVRVLAPLVNDKFSDPAVVVIDERGQHVISLLSGHAGGANALTR 117 Query: 114 RVAQCTGAQPVVTTATE 130 +A GA PV+TTAT+ Sbjct: 118 YLAGMLGADPVITTATD 134 >FN0952 [H] COG2073 Cobalamin biosynthesis protein CbiG Fnu Fusobacterium nucleatum Length = 337 Score = 77.4 bits (189), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 43/117 (36%), Positives = 65/117 (55%), Gaps = 2/117 (1%) Query: 133 KPLVLGIGCRKLASAEQIGRAVAQALAQCPGATLAQVREVATIDLKAEEPGLLAFCATHG 192 K L+LGIGC+K AE I A+ L + + V++VAT+D+K E GL+ Sbjct: 207 KNLILGIGCKKDIKAEDILSAIEDCLDK-NNLDIKSVKKVATVDVKENEKGLIDAVKFLN 265 Query: 193 LPLRVIARAQIAQRAWVGKPSDWVRQNVGVEGVCEPAALIASP-RGRLLLGKMALDG 248 L L +I+R +I + SD+V +N+GV V EP AL++S G+ L+ K +G Sbjct: 266 LDLEIISREEIKKVQDQFDGSDFVEKNIGVRAVSEPVALLSSTGNGKFLVMKEKCNG 322 Score = 66.6 bits (161), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 42/147 (28%), Positives = 68/147 (46%), Gaps = 11/147 (7%) Query: 9 ELGLWPVRAEAAALAMLLQDSLGGELYQPWLN-------DQGGLSQRQQFAL----AYRR 57 +L W V A +A ++ L L ++ D Q + F + + Sbjct: 2 KLAFWTVTKGAGNIAREYKEKLKEHLKDYEIDVFTLKKYDVENTIQIKDFTCNINEKFSQ 61 Query: 58 HRQWIMIGATGIAVRFLDGLPQDKHSDPAVVVVDEAGRFAIALLAGHEGGANSLAYRVAQ 117 + I I A+GI +R + L K DPAV+++DE F I+LL+GH G AN L Y +A Sbjct: 62 YDGHIFIMASGIVIRKIASLIDTKDKDPAVLLIDEGKHFVISLLSGHLGRANELTYSLAN 121 Query: 118 CTGAQPVVTTATEAVKPLVLGIGCRKL 144 PV+TT+++ + + +KL Sbjct: 122 ILKLVPVITTSSDVTGKIAVDTISQKL 148 >CAC1370 [H] COG2073 Cobalamin biosynthesis protein CbiG Cac Clostridium acetobutylicum Length = 326 Score = 73.9 bits (180), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 36/109 (33%), Positives = 61/109 (55%), Gaps = 6/109 (5%) Query: 28 DSLGGELYQPWLNDQGGLSQRQQFALA------YRRHRQWIMIGATGIAVRFLDGLPQDK 81 D +G +L Q D + + F L+ R+ I + +TGIAVR + + K Sbjct: 13 DKIGKKLRQAMDLDIYSKREIENFKLSTLTEMLINRYEALIFVASTGIAVRAIAPFIKSK 72 Query: 82 HSDPAVVVVDEAGRFAIALLAGHEGGANSLAYRVAQCTGAQPVVTTATE 130 DPA++VVD ++ I+LL+GH G AN + ++++ G+ P++TTAT+ Sbjct: 73 TEDPAIIVVDVLSKYTISLLSGHIGRANEMTLKISKILGSIPIITTATD 121 Score = 72.4 bits (176), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 36/121 (29%), Positives = 66/121 (54%), Gaps = 3/121 (2%) Query: 133 KPLVLGIGCRKLASAEQIGRAVAQALAQCPGATLAQVREVATIDLKAEEPGLLAFCATHG 192 K ++LGIGCRK S E + V + L + G V+ +++ID+KA E ++ Sbjct: 202 KNVILGIGCRKNYSVEAMENIVMKKLREL-GIDKRAVKTISSIDIKANEKAIIHLSEVLK 260 Query: 193 LPLRVIARAQIAQRAWVGKPSDWVRQNVGVEGVCEPAALIASPRGRLLLGKMALDGVTVA 252 + + +I + + SD+V +NVGV VCEP +++ G+++ KM ++G+T+ Sbjct: 261 ADFKTFTKEEIKTVQEIYEGSDFVEKNVGVRSVCEPCVKLSN--GQIVFSKMKINGMTLC 318 Query: 253 V 253 + Sbjct: 319 I 319 >MTH1408 [H] COG2073 Cobalamin biosynthesis protein CbiG Mth Methanothermobacter thermautotrophicus Length = 329 Score = 70.9 bits (172), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 44/106 (41%), Positives = 59/106 (55%), Gaps = 5/106 (4%) Query: 30 LGGELYQPWLNDQGGLS---QRQQFALAYRRHRQWIMIG--ATGIAVRFLDGLPQDKHSD 84 L GE+ + L D LS + L I++G A GI VR + L +K SD Sbjct: 15 LAGEMREKLLRDPTILSVEVMHRNMDLGNVFAESDIIVGIMAAGIMVRKIAPLINNKLSD 74 Query: 85 PAVVVVDEAGRFAIALLAGHEGGANSLAYRVAQCTGAQPVVTTATE 130 P V+VVDE G I+LL+GH GGAN A ++A GA PV+TT+T+ Sbjct: 75 PGVLVVDEKGSNVISLLSGHAGGANDFAIKLAGLIGANPVITTSTD 120 >slr0969_1 [H] COG2073 Cobalamin biosynthesis protein CbiG Syn Synechocystis Length = 370 Score = 61.6 bits (148), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 42/105 (40%), Positives = 59/105 (56%), Gaps = 2/105 (1%) Query: 24 MLLQDSLGGELY--QPWLNDQGGLSQRQQFALAYRRHRQWIMIGATGIAVRFLDGLPQDK 81 +LL SL EL + L + G S A + H+ +I ATG VR + L Q K Sbjct: 43 LLLSKSLAEELELGKNSLVETYGDSLATAVAERWNNHQAFIFGLATGAVVRLIAPLLQSK 102 Query: 82 HSDPAVVVVDEAGRFAIALLAGHEGGANSLAYRVAQCTGAQPVVT 126 SDPAV+VVD G+ I+LL+GH+GG + LA VA+ A+ ++T Sbjct: 103 QSDPAVLVVDAMGQSVISLLSGHQGGGDRLAQIVAEQLQAKAILT 147