BLASTP 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0001.0062.p (252 letters) Database: COGmodified 192,987 sequences; 59,019,183 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0621 [H] COG2243 Precorrin-2 methylase Rso Ralstonia solanace... 323 2e-88 FN0959 [H] COG2243 Precorrin-2 methylase Fnu Fusobacterium nucle... 100 4e-21 MTH1348 [H] COG2243 Precorrin-2 methylase Mth Methanothermobacte... 97 3e-20 CAC1379 [H] COG2243 Precorrin-2 methylase Cac Clostridium acetob... 95 2e-19 STM2024 [H] COG2243 Precorrin-2 methylase Sty Salmonella typhimu... 89 8e-18 lin1166 [H] COG2243 Precorrin-2 methylase Lin Listeria innocua 79 1e-14 MJ0771 [H] COG2243 Precorrin-2 methylase Mja Methanococcus janna... 77 4e-14 PAE0348 [H] COG2243 Precorrin-2 methylase Pya Pyrobaculum aeroph... 72 9e-13 slr1879 [H] COG2243 Precorrin-2 methylase Syn Synechocystis 72 1e-12 all0455 [H] COG2243 Precorrin-2 methylase Nos Nostoc sp. PCC 7120 69 1e-11 >RSp0621 [H] COG2243 Precorrin-2 methylase Rso Ralstonia solanacearum Length = 243 Score = 323 bits (828), Expect = 2e-88, Method: Compositional matrix adjust. Identities = 161/239 (67%), Positives = 192/239 (80%), Gaps = 1/239 (0%) Query: 3 GRLTGIGVGPGPAGYLPLAAIAALRSADLIYIPRARGADLSVALQCLDGVEFDRSRLCEI 62 GR TGIGVGPGPAG +P+AA+ AL +ADLIY+PRAR ++ SVA QCL G+ ++L EI Sbjct: 5 GRFTGIGVGPGPAGLIPVAALEALNAADLIYLPRARDSEASVARQCLAGLAVPDTKLREI 64 Query: 63 EFNMDPDRQVLQEHYAQLGQQLAAQLRQGLQVVYLTIGDALTYSTYGYVLAAVRDLYPAC 122 F MDPDR VL +HYA+L +AA+LR G V YLT+GD+LTYSTYGY+LAA+RD PA Sbjct: 65 AFQMDPDRSVLSQHYAELAGTVAAELRAGRNVAYLTLGDSLTYSTYGYLLAALRDALPAL 124 Query: 123 DCVTFPGITSYAAAAAALEWPLGVGKERTLILPCPEDAASLRAEIESHDIVVLMKVGARL 182 + VTFPGITS+AA A+AL WPLG GKER LILPCP+D +LRA+I SHDIVVLMK+GARL Sbjct: 125 EHVTFPGITSFAAVASALSWPLGEGKERILILPCPDDMEALRADIASHDIVVLMKIGARL 184 Query: 183 PWVLALLREMGIAQHCGFARRIGLEGEVLASDLTALDAADASGYLAVLLIRKTAQAQRH 241 P VL LL MGIAQHC FARRIGL GEVL D+ AL +ADASGYLA +LIR+TA+ +RH Sbjct: 185 PAVLGLLNAMGIAQHCAFARRIGLPGEVLCDDVRAL-SADASGYLATMLIRRTAREKRH 242 >FN0959 [H] COG2243 Precorrin-2 methylase Fnu Fusobacterium nucleatum Length = 248 Score = 100 bits (248), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 68/237 (28%), Positives = 113/237 (47%), Gaps = 11/237 (4%) Query: 1 MSGRLTGIGVGPGPAGYLPLAAIAALRSADLIYIPRARGADLSVALQCLDGVEFDRSRLC 60 M+ + GIGVG G + + AI L+ D++ +P A+ D SVA + + Sbjct: 9 MNNKFYGIGVGVGDPEEITIKAINTLKKLDVVILPEAKKDDGSVAYEIAKQYMKEDVEKI 68 Query: 61 EIEFNMDP---DRQVLQEHYAQLGQQLAAQLRQGLQVVYLTIGDALTYSTYGYVLAAVRD 117 +EF M DR+ ++ ++ Q+L L +G V +LTIGD +TYSTY Y+L + + Sbjct: 69 FVEFPMLKSLEDRENARKENTKIVQKL---LDEGKNVGFLTIGDTMTYSTYVYILEHLPE 125 Query: 118 LYPACDCVTFPGITSYAAAAAALEWPLGVGKERTLILPCPEDAASLRAEIESHDIVVLMK 177 Y T PG++S+ A+ +PL +G E TL + ++ E+E++D +V MK Sbjct: 126 KYLVE---TVPGVSSFVDMASRFNFPLMIGDE-TLKVVSLNKKTNIEFELENNDNIVFMK 181 Query: 178 VGARLPWVLALLREMGIAQHCGFARRIGLEGEVLASDLTALDAADASGYLAVLLIRK 234 V + L + G G E + + D+ L D Y L+++K Sbjct: 182 VSRNFENLKQALIKTGNIDKIIMVSNCGKENQKVYYDIKDLTEDDIP-YFTTLIVKK 237 >MTH1348 [H] COG2243 Precorrin-2 methylase Mth Methanothermobacter thermautotrophicus Length = 232 Score = 97.1 bits (240), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 71/241 (29%), Positives = 111/241 (46%), Gaps = 12/241 (4%) Query: 1 MSGRLTGIGVGPGPAGYLPLAAIAALRSADLIYIPRARGADLSVALQCLDGVEFDRSRLC 60 M G+L G+GVGPG + L L A+ LRS +I PR+ S+AL ++ + +R C Sbjct: 1 MHGKLIGVGVGPGDSELLTLRAVNVLRSVPVICAPRSSSERESIALSIVEDILTERRDGC 60 Query: 61 EI---EFNMDPDRQVLQEHYAQLGQQLAAQLRQGLQVVYLTIGDALTYSTYGYVLAAVRD 117 I F M DR L+ H+ + +AA+L G V ++T+GD YST+ Y+ + D Sbjct: 61 RILDPVFPMTDDRDELESHWDSAARMVAAELEDGRDVAFITLGDPSIYSTFSYLQQRIED 120 Query: 118 LYPACDCVTFPGITSYAAAAAALEWPLGVGKERTLILPCPEDAASLRAEIESHDIVVLMK 177 + + V PG+TS+ A AA L G E L++P +D + D V+MK Sbjct: 121 MGFKTEMV--PGVTSFTACAATAGRTLVEGDEILLVVPRVDD--RFERVLRDVDACVIMK 176 Query: 178 VGARLPWVLALLREMGIAQHCGFARRIGLEGEVLASDLTALDAADASGYLAVLLIRKTAQ 237 + ++ + ++ EV+ A GYLA L+R Q Sbjct: 177 TSRHGRRAMEVVESDPRGKDVVSVANCSMDDEVVERGF-----ASGGGYLATTLVRFREQ 231 Query: 238 A 238 + Sbjct: 232 S 232 >CAC1379 [H] COG2243 Precorrin-2 methylase Cac Clostridium acetobutylicum Length = 221 Score = 94.7 bits (234), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 67/206 (32%), Positives = 100/206 (48%), Gaps = 11/206 (5%) Query: 4 RLTGIGVGPGPAGYLPLAAIAALRSADLIYIPRARGADLSVALQCLDGVEFDRSRLCEIE 63 ++ GIGVGPG + L + A+ A++ ++I P A S+AL + + + Sbjct: 3 KIYGIGVGPGDSELLTIKAVKAIKKCEVIVAPSAMKGKRSIALNIAKEYINKDAEIMVLH 62 Query: 64 FNMDPDRQVLQEHYAQLGQQLAAQLRQGLQVVYLTIGDALTYSTYGYVLAAVRDLYPACD 123 F M + Q +E + + +L +G V +LTIGD YSTY Y+L + D Sbjct: 63 FPMGGEEQ--EEKIYDAFKAVEEKLLEGKNVGFLTIGDPFVYSTYIYLLKHIED--KGYQ 118 Query: 124 CVTFPGITSYAAAAAALEWPLGVGKERTLILPCPEDAASLRAEIESHDIVVLMKVGARLP 183 T PGITS++A A+ PL +G E LI+P L A I+ VV+MKV R Sbjct: 119 TETIPGITSFSACASLANEPLVIGDEPLLIIP----GNRLEA-IKDEKNVVIMKVYKREE 173 Query: 184 WVLALLREMGIAQHCGFARRIGLEGE 209 +L L E G C ++ G EG+ Sbjct: 174 EILNTLEEKGFNYVC--VKKAGREGQ 197 >STM2024 [H] COG2243 Precorrin-2 methylase Sty Salmonella typhimurium LT2 Length = 237 Score = 89.4 bits (220), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 63/238 (26%), Positives = 113/238 (47%), Gaps = 11/238 (4%) Query: 1 MSGRLTGIGVGPGPAGYLPLAAIAALRSADLIYIPRARGADLSVALQCLDGVEFDRSRLC 60 M+G+L + GPG + + A L S D++Y P R S+AL + +++ + Sbjct: 1 MNGKLYALSTGPGAPDLITVRAARILGSLDILYAPAGRKGGDSLALSIVRDYLGEQTEVR 60 Query: 61 EIEFNMDPDRQVLQEHYAQLGQQLAAQLRQGLQVVYLTIGDALTYSTYGYVLAAVRDLYP 120 F M D + + ++ L A++ G QV ++T+GDA+ +ST+ ++L + Sbjct: 61 CCHFPMSADGAEKEAVWNEVAAALTAEVEAGKQVGFITLGDAMLFSTWIFLLQRI----- 115 Query: 121 ACDCVTF----PGITSYAAAAAALEWPLGVGKERTLILPCPEDAASLRAEIESHDIVVLM 176 C + PG+TS+AA AA + PL + ++ ++ C A + ++ HD +VLM Sbjct: 116 --GCPEWLEIVPGVTSFAAIAARAKMPLAIERQSLAVISCTAPEAEIAQALQQHDSLVLM 173 Query: 177 KVGARLPWVLALLREMGIAQHCGFARRIGLEGEVLASDLTALDAADASGYLAVLLIRK 234 KV R + ALL + G+ + L GE L ++ Y + +L+ K Sbjct: 174 KVYGRFARIKALLAQAGLLECALMMSEATLPGEQCWRHLHEVNDDRPLPYFSTILVNK 231 >lin1166 [H] COG2243 Precorrin-2 methylase Lin Listeria innocua Length = 236 Score = 78.6 bits (192), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 43/174 (24%), Positives = 89/174 (51%), Gaps = 3/174 (1%) Query: 4 RLTGIGVGPGPAGYLPLAAIAALRSADLIYIPRARGADLSVALQCLDGVEFDRSRLCEIE 63 + GIG GPG + + + A L ++Y P+++ S+A + ++ + + E Sbjct: 3 KFYGIGTGPGDSKLVTMEAADRLGKLAILYTPQSKKGGESLAKKIVNPYLKNTLIIKERH 62 Query: 64 FNMDPDRQVLQEHYAQLGQQLAAQLRQGLQVVYLTIGDALTYSTYGYVLAAVRDLYPACD 123 F M ++ + + ++ +++ ++ G V ++T+GD + YSTY Y+L ++ + Sbjct: 63 FPMSYNKTEKMQAWKEIAEEIKTDVQNGHDVGFITLGDPMVYSTYSYLLELLKG---EIE 119 Query: 124 CVTFPGITSYAAAAAALEWPLGVGKERTLILPCPEDAASLRAEIESHDIVVLMK 177 VT GI+S++ A+ LE PL + ++ ++P A + + D +VLMK Sbjct: 120 TVTLAGISSFSNIASKLELPLVMDEDSFAVIPVTAGAEKIEQALTLFDTIVLMK 173 >MJ0771 [H] COG2243 Precorrin-2 methylase Mja Methanococcus jannaschii Length = 230 Score = 77.0 bits (188), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 60/218 (27%), Positives = 109/218 (50%), Gaps = 12/218 (5%) Query: 18 LPLAAIAALRSADLIYIPRARGADLSVALQCLDGVEFDRSRLCEIEFNMDPDRQVLQEHY 77 L L A+ L+ D I++P ++ S+A + + D + E+ F M D++ L++++ Sbjct: 21 LTLKALEVLKKVDKIFVPVSKKGKKSIAYEIIKDY-VDGKNIEELLFPMIKDKERLKKYW 79 Query: 78 AQLGQQLAAQLRQGLQVVYLTIGDALTYSTYGYVLAAVRDLYPACDCVTFPGITSYAAAA 137 L L++ +V +TIGD YST+ YV +++ + V GI+S A+A Sbjct: 80 E---NALEKVLKEDGEVAIITIGDPTLYSTFSYVWKLLKERGVEVEIVN--GISSIFASA 134 Query: 138 AALEWPLGVGKERTLILPCPEDAASLRAEIESHDIVVLMKVGARLPWVLALLREMGIAQH 197 AAL PL G E+ ILP +D L I+ D +++MK L L++++ Sbjct: 135 AALNIPLVEGDEKLCILPQGKD---LEKYIDEFDTIIIMKT-KNLNEKLSVIKNRD-DYI 189 Query: 198 CGFARRIGLEGE-VLASDLTALDAADASGYLAVLLIRK 234 G +R E E V+ L ++ + + YL++ +I++ Sbjct: 190 IGLVKRATFEDEKVVIGKLDEINFDEFNDYLSLAIIKR 227 >PAE0348 [H] COG2243 Precorrin-2 methylase Pya Pyrobaculum aerophilum Length = 208 Score = 72.4 bits (176), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 62/213 (29%), Positives = 105/213 (49%), Gaps = 21/213 (9%) Query: 8 IGVGPGPAGYLPLAAIAALRSADLIYIPRARGADLSVALQCLDGVEFDRSRLCEIEFNMD 67 +G+GPG L + AI L AD+IY+PR+ ++ S+A + +++ R R+ E+ FNM Sbjct: 5 VGLGPGDPELLTIKAIRLLSKADVIYVPRSSRSEASLAKNIV--LKYARGRVVELPFNMG 62 Query: 68 PDRQVLQEHYAQLGQQLAAQLRQGLQVVYLTIGDALTYSTYGYVLAAVRDLYPACDCVTF 127 R+ QL +++A + G VY +GD YST+G + Y Sbjct: 63 GVRE------EQL-REIAKIISSGENAVYAVLGDPSLYSTFGKIRK-----YVDRPVEYV 110 Query: 128 PGITSYAAAAAALEWPLGVGKERTLILPCPEDAASLRAEIESHDIVVLMKVGARLPWVLA 187 PG+++ + + L VG I+P A L+ + D+VV+ K + Sbjct: 111 PGVSALISCSLRAGVELAVGDMAVAIVPASR-ADLLKEALRLFDVVVVAKANRNIE---- 165 Query: 188 LLREMGIAQHCGFA-RRIGLEGEVLASDLTALD 219 LL E+ ++ H G+A RR +EGE +++ +T D Sbjct: 166 LLNEL-LSSHGGYAVRRCYMEGEAVSNRITWSD 197 >slr1879 [H] COG2243 Precorrin-2 methylase Syn Synechocystis Length = 242 Score = 71.6 bits (174), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 57/236 (24%), Positives = 104/236 (44%), Gaps = 7/236 (2%) Query: 3 GRLTGIGVGPGPAGYLPLAAIAALRSADLIYIPRARGADLSVALQCLDGVEFDRSRLCEI 62 G L G+ VGPG + L + L+S ++ P VA Q ++ + Sbjct: 4 GILHGVSVGPGDPELITLKGLKILQSCPVVAFPAGLQGRSGVAEQIINSYLKPEQIRLSL 63 Query: 63 EFNMDPDRQVLQEHYAQLGQQLAAQLRQGLQVVYLTIGDALTYSTYGYVLAAVRDLYPAC 122 +F ++L++ + + ++ L +G+ V + GD YST+ Y+ ++ +P Sbjct: 64 KFPYVQKEEILEQAWQEAAAEVWPYLEKGMDVAFACEGDISFYSTFTYLAQTLKSNHPQL 123 Query: 123 DCVTFPGITSYAAAAAALEWPLGVGKERTLILP---CPEDAASLRAEIESHDIVVLMKVG 179 G++S A A+ L PL +E+ ILP CPE+ L ++ ++VVLMKV Sbjct: 124 GINYIAGVSSPFAGASTLGLPLTCQEEKLAILPALYCPEE---LEKALDWAEVVVLMKVR 180 Query: 180 ARLPWVLALLREMGIAQHCGFARRIGLEGEVLASDLTALDAADASGYLAVLLIRKT 235 V +L + G+ +I E + L+ S Y +++LI++ Sbjct: 181 LVYGQVWKILAKRGLLDQAWLLEKISTAEEKIYHPLSHYPQLSLS-YFSLMLIKQN 235 >all0455 [H] COG2243 Precorrin-2 methylase Nos Nostoc sp. PCC 7120 Length = 234 Score = 68.9 bits (167), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 62/236 (26%), Positives = 104/236 (44%), Gaps = 16/236 (6%) Query: 2 SGRLTGIGVGPGPAGYLPLAAIAALRSADLIYIPRARGAD---LSVALQCLDGVEFDRSR 58 GRL G+GVGPG L + A+ LR+A +I A + ++ Q L G + + + Sbjct: 4 KGRLYGVGVGPGDPELLTIKALRLLRAAPVIAYQSATDKESIARAIVSQYLTGEQIEVA- 62 Query: 59 LCEIEFNMDPDRQVLQEHYAQLGQQLAAQLRQGLQVVYLTIGDALTYSTYGYVLAAVRDL 118 + ++P++ QE Y Q +A L G VV L GD Y ++ YV + + Sbjct: 63 -YHLPRALEPEKA--QEIYDQEVAPIAEHLAAGRDVVVLCEGDPFFYGSFMYVFTRLSEK 119 Query: 119 YPACDCVTFPGITSYAAAAAALEWPLGVGKERTLILPCPEDAASLRAEIESHDIVVLMKV 178 Y PG++S A +L P + +LP P A L +++ + D +MK+ Sbjct: 120 Y---QTEVVPGVSSLMACPVSLGVPFTYYNDILTVLPAPLPAEELTSKLLTTDAAAIMKL 176 Query: 179 GARLPWVLALLREMGIAQHCGFARRIGLEGEVLASDLTALDAADAS--GYLAVLLI 232 G V +L ++G+A + R + LD D + Y A+++I Sbjct: 177 GRHFTKVRDVLHQLGLASRALYIER----ATTTQQRIIPLDEVDPAEVPYFAMIVI 228