BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0189.p (370 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311313|ref|YP_003775405.1| acyltransferase [Herbaspirillum... 694 0.0 gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspiri... 79 9e-16 gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspiri... 77 3e-15 gi|300312886|ref|YP_003776978.1| acetyltransferase [Herbaspirill... 74 2e-14 gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspiri... 68 1e-12 >gi|300311313|ref|YP_003775405.1| acyltransferase [Herbaspirillum seropedicae SmR1] Length = 350 Score = 694 bits (1792), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/350 (99%), Positives = 350/350 (100%) Query: 21 MVHTQHKEIPSLDGWRALAIAIVFLSHAGLGRIVPGGLGVTIFFFLSGYLITTLLVREFD 80 MVHTQHKEIPSLDGWRALAIAIVFLSHAGLGRIVPGGLGVTIFFFLSGYLITTLLVREFD Sbjct: 1 MVHTQHKEIPSLDGWRALAIAIVFLSHAGLGRIVPGGLGVTIFFFLSGYLITTLLVREFD 60 Query: 81 RSDSVNVRHFYLRRLLRLTPPLLLVLVLTYTLTWLGIFTGDASWSGFLAQLFYFANYYVL 140 RSDSVNVRHFYLRRLLRLTPPLLLVLVLTYTLTWLGIFTGDASWSGFLAQLFYFANYYVL Sbjct: 61 RSDSVNVRHFYLRRLLRLTPPLLLVLVLTYTLTWLGIFTGDASWSGFLAQLFYFANYYVL 120 Query: 141 FFDPGHTLPQGTGVFWSLAVEEHFYFVFPALFIFLMHKADRRKIPLYLGGLCLLVLLWRY 200 FFDPGHTLPQGTGVFWSLAVEEHFYFVFPALFIFLMHKADRRKIPLYLGGLCLLVLLWRY Sbjct: 121 FFDPGHTLPQGTGVFWSLAVEEHFYFVFPALFIFLMHKADRRKIPLYLGGLCLLVLLWRY 180 Query: 201 GLVLLGDASMDRTYYATDTRIDSILFGCILATLKLPPAVYAALHKPAVRVGGVTLGVVLL 260 GLVLLGDASMDRTYYATDTRIDSILFGCILATLKLPPAVYAALHKPAVRVGGVTLGV+LL Sbjct: 181 GLVLLGDASMDRTYYATDTRIDSILFGCILATLKLPPAVYAALHKPAVRVGGVTLGVLLL 240 Query: 261 LLSLAVRDEVFRETLRYSVQGLALMPLFFYSVAHPHSFPFRLLNLRWVQRIGIYSYSIYL 320 LLSLAVRDEVFRETLRYSVQGLALMPLFFYSVAHPHSFPFRLLNLRWVQRIGIYSYSIYL Sbjct: 241 LLSLAVRDEVFRETLRYSVQGLALMPLFFYSVAHPHSFPFRLLNLRWVQRIGIYSYSIYL 300 Query: 321 SHEILLSNVDWVSHNAAVNIAVAAVFSLLFAMAVDRCIDAPLRVLRARFR 370 SHEILLSNVDWVSHNAAVNIAVAAVFSLLFAMAVDRCIDAPLR+LRARFR Sbjct: 301 SHEILLSNVDWVSHNAAVNIAVAAVFSLLFAMAVDRCIDAPLRMLRARFR 350 >gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 681 Score = 78.6 bits (192), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 69/214 (32%), Positives = 110/214 (51%), Gaps = 25/214 (11%) Query: 30 PSLDGWRALAIAIVFLSHAGLGRIVPGG-LGVTIFFFLSGYLITTLLVREFDRSDSVNVR 88 P +DG RA+AI V + HA +PGG +GV IFF +SG+LI++++ R+ D+ + Sbjct: 34 PDIDGLRAIAIVSVVVFHA-FPDAMPGGFIGVDIFFVISGFLISSIIFRQLDQG-RFSFT 91 Query: 89 HFYLRRLLRLTPPLLLVLVLTYTLTWLGIFTGD--------ASWSGFLAQLFYF--ANYY 138 FY+ R+ R+ P L+LVL + L W + + A+ +GF+ L + + Y+ Sbjct: 92 DFYVHRIRRIFPALILVLASCFVLGWFSLLPEEYRQLGKHMAAGAGFIQNLVLWKESGYF 151 Query: 139 VLFFDPGHTLPQGTGVFWSLAVEEHFYFVFPALFIFLMHKADRRKIPLYLGGLCLLVLLW 198 + D L WSL+VEE FY V+P L L R ++PL +L++L Sbjct: 152 DVESDMKPLL-----HLWSLSVEEQFYLVYPLLVCGLW----RARMPLR--NSLVLLMLA 200 Query: 199 RYGLVLLGDASMD-RTYYATDTRIDSILFGCILA 231 + L L A +T+Y TR+ +L G + A Sbjct: 201 SFALNLWWVAQDPVKTFYLPFTRVWELLAGALCA 234 >gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 680 Score = 77.0 bits (188), Expect = 3e-15, Method: Compositional matrix adjust. Identities = 67/221 (30%), Positives = 107/221 (48%), Gaps = 23/221 (10%) Query: 21 MVHTQHKEIPSLDGWRALAIAIVFLSHAGLGRIVPGG-LGVTIFFFLSGYLITTLLVREF 79 + H++++ +DG RA+A+ V H G V GG +GV +FF +SGYLI+T++ Sbjct: 9 LSHSKYRS--DIDGLRAIAVLAVVSFH-GFPNFVRGGFIGVDVFFVISGYLISTIIFENL 65 Query: 80 DRSDSVNVRHFYLRRLLRLTPPLLLVLVLTYTLTWLGIFTGD--------ASWSGFLAQL 131 DR + FY RR+ R+ P LLLVL + + W + T + A +GF++ Sbjct: 66 DRG-TFTFTEFYARRVRRIFPALLLVLSTCFVVGWFVLLTDEYKQLGKHIAGGAGFVSN- 123 Query: 132 FYFANYYVLFFDPGHTLPQGTGVFWSLAVEEHFYFVFPALFIFLMHKADRRKIPLYLGGL 191 F + F + T P WSL +EE FY ++P L F + RRK L + Sbjct: 124 FLLRDESGYFDNSAETKPLLH--LWSLGIEEQFYILWPMLLFF----SWRRKFNLLT--I 175 Query: 192 CLLVLLWRYGLVLLG-DASMDRTYYATDTRIDSILFGCILA 231 ++V L + + G + +Y +R +L G +LA Sbjct: 176 TIIVALASFAANIKGINKDATAAFYLPQSRFWELLSGTLLA 216 >gi|300312886|ref|YP_003776978.1| acetyltransferase [Herbaspirillum seropedicae SmR1] Length = 364 Score = 73.9 bits (180), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 97/328 (29%), Positives = 141/328 (42%), Gaps = 37/328 (11%) Query: 56 GGLGVTIFFFLSGYLITTLLVREFDRSDSVNVRHFYLRRLLRLTPPLLLVLVLTYTLTWL 115 G GVTIFF +SGYL+T +R + R D+++ R FY R R+ P + L+L + L L Sbjct: 53 GNYGVTIFFVISGYLMTANTLRRWGRLDAIDARRFYALRAWRILPCMFLLLAMVSLLAAL 112 Query: 116 G--IFTGDA-------SWSGFLAQLFYFANYYVLFFDPGHTLPQGTGVFWSLAVEEHFYF 166 G +F W LA L ++ N VL G + GV WSL+VE FY Sbjct: 113 GGTLFQNRGFGEPPLPMWMTALASLTFWMN--VLIAQHGW-VNYPLGVLWSLSVEGVFYL 169 Query: 167 VFPALFIFLMHKADRRKIPLYLGGLCLLVLLWR--YGLVLLGDASMDRTYYATDTRIDSI 224 FP + + L RR PL++G CLL ++ Y L GD YA D I Sbjct: 170 AFPVVCLTL-----RR--PLFIGAFCLLFIVAGPLYRLAHQGDE--GGFLYAYLACFDGI 220 Query: 225 LFGCILATLKLPPAVYAALHKPAVRVGGVTLGVVLLLLSLAVRDEVFRETLRYSVQGLAL 284 GC A L V H P + + +L + L + + R + V +AL Sbjct: 221 AIGCCTAIL-----VARGTH-PWISTRWLQCMTILAMALLYLCWPISRSNV-LGVTAMAL 273 Query: 285 MPLFFYSVAHPHSFPFRLLNLRWVQRIGIYSYSIYLSHEILL----SNVDWVSHNAAVNI 340 + A + R ++ G SY IYL H I+L + + ++ ++ V + Sbjct: 274 GTALLLAGASGKTVAARSAGYSFLAFCGGLSYEIYLFHLIVLGLMRTAIPPITASSEVKL 333 Query: 341 AVAA---VFSLLFAMAVDRCIDAPLRVL 365 + A + SL A AV R P L Sbjct: 334 GLLAGYLICSLGVACAVARFFSRPFNPL 361 >gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 662 Score = 68.2 bits (165), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 90/316 (28%), Positives = 143/316 (45%), Gaps = 42/316 (13%) Query: 30 PSLDGWRALAIAIVFLSHAGLGRIVPGGLGVTIFFFLSGYLITTLLVREFDRSDSVNVRH 89 P +DG RA+A+ V + HA G +GV IFF +SG+LI+ +L + + ++ Sbjct: 21 PDIDGLRAVAVGSVVIFHAFPEWFAGGFIGVDIFFVISGFLISLILFKNLEHG-RFSIVD 79 Query: 90 FYLRRLLRLTPPLLLVLVLTYTLTWLGIFTGDASWSG--------FLAQ-LFYFANYYVL 140 FY+RR+ R+ P L+ V+ L W +F + G F++ LF+ + Y Sbjct: 80 FYVRRVRRIFPALMTVMSLCLVTGWFVLFADEYKQLGKHLFGGSTFISNFLFWRESGY-- 137 Query: 141 FFDPGHTLPQGTGVFWSLAVEEHFYFVFPALFIFLMHKADRRKIPLYLGGLCLLVLL-WR 199 F + T P WSLA+EE FY +P L F+ +R+ +LG + ++ LL + Sbjct: 138 FDNSAETKPLLH--LWSLAIEEQFYLFWPLLLAFVW----KRRWG-FLGIMGVIGLLSFG 190 Query: 200 YGLVLLGDASMDRTYYATDTRIDSILFGCILATLKLPPAVYAALHKPAVRVG----GVTL 255 L +G +Y+ R ++ G LA Y LH+ + G L Sbjct: 191 VNLYFIGH-DQSAAFYSPLARFWELMIGGALA--------YINLHRQDLTRGYKNLQAWL 241 Query: 256 GVVLLLLSLAVRDEVFRETLRYSVQGLALMP---LFFYSVAHPHS-FPFRLLNLRWVQRI 311 G LL+ A+ D R AL+P F A P + F R+L + + + Sbjct: 242 GAALLVAGFALLDGA-----RAFPGWWALLPSLGAFLLLSAGPGAWFNRRVLASKGMVAL 296 Query: 312 GIYSYSIYLSHEILLS 327 G+ SY +YL H LL+ Sbjct: 297 GLVSYPLYLWHWPLLA 312 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.332 0.145 0.458 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 965,679 Number of extensions: 34749 Number of successful extensions: 154 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 144 Number of HSP's successfully gapped: 6 Length of query: 370 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 283 Effective length of database: 1,207,925 Effective search space: 341842775 Effective search space used: 341842775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 131 (55.1 bits)