BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0166.p (749 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311292|ref|YP_003775384.1| (p)ppGpp synthetase I [Herbaspi... 1538 0.0 gi|300311444|ref|YP_003775536.1| bifunctional (P)ppGpp synthetas... 365 e-102 >gi|300311292|ref|YP_003775384.1| (p)ppGpp synthetase I [Herbaspirillum seropedicae SmR1] Length = 749 Score = 1538 bits (3983), Expect = 0.0, Method: Compositional matrix adjust. Identities = 748/749 (99%), Positives = 748/749 (99%) Query: 1 MVSVASTQGEGLAALTAGLGPEDAARVAQALAELEPLYADRQILSGQNALQFSISVATVL 60 MVSVASTQGEGLAALTAGLGPEDAARVAQALAELEPLYADRQILSGQNALQFSISVATVL Sbjct: 1 MVSVASTQGEGLAALTAGLGPEDAARVAQALAELEPLYADRQILSGQNALQFSISVATVL 60 Query: 61 TALNVDAATRIAALAFEVQMLHPELEEKLEERFGKEIADLVGNVRQLMRFHSFSFQPQQE 120 TALNVDAATRIAALAFE QMLHPELEEKLEERFGKEIADLVGNVRQLMRFHSFSFQPQQE Sbjct: 61 TALNVDAATRIAALAFEAQMLHPELEEKLEERFGKEIADLVGNVRQLMRFHSFSFQPQQE 120 Query: 121 VLRGKNAAQQAAAQIETLRKMLLAMATDMRAVLVRLASRVATLRYFAETKQEDEASANYA 180 VLRGKNAAQQAAAQIETLRKMLLAMATDMRAVLVRLASRVATLRYFAETKQEDEASANYA Sbjct: 121 VLRGKNAAQQAAAQIETLRKMLLAMATDMRAVLVRLASRVATLRYFAETKQEDEASANYA 180 Query: 181 RQTFELYAPLANRLGIWQLKWELEDLSFRFLEPVTYKRIAKMLEEKRLEREAFVANAIAR 240 RQTFELYAPLANRLGIWQLKWELEDLSFRFLEPVTYKRIAKMLEEKRLEREAFVANAIAR Sbjct: 181 RQTFELYAPLANRLGIWQLKWELEDLSFRFLEPVTYKRIAKMLEEKRLEREAFVANAIAR 240 Query: 241 LRAEMAAQDIRAEVSGRPKHIYSIWNKMRGKHIDFSELYDVRAFRVIVDDVKTCYAVLGI 300 LRAEMAAQDIRAEVSGRPKHIYSIWNKMRGKHIDFSELYDVRAFRVIVDDVKTCYAVLGI Sbjct: 241 LRAEMAAQDIRAEVSGRPKHIYSIWNKMRGKHIDFSELYDVRAFRVIVDDVKTCYAVLGI 300 Query: 301 VHDIWTPIPEEFDDYISRPKQNGYQSLHTVVTAEDGRALEVQIRTHEMHHFAEYGVAAHW 360 VHDIWTPIPEEFDDYISRPKQNGYQSLHTVVTAEDGRALEVQIRTHEMHHFAEYGVAAHW Sbjct: 301 VHDIWTPIPEEFDDYISRPKQNGYQSLHTVVTAEDGRALEVQIRTHEMHHFAEYGVAAHW 360 Query: 361 RYKESGGSNFSAQKYDEKIAWLRQLLAWKNEVTDAVVDQEEVRRDWVEKLKSATLDERIY 420 RYKESGGSNFSAQKYDEKIAWLRQLLAWKNEVTDAVVDQEEVRRDWVEKLKSATLDERIY Sbjct: 361 RYKESGGSNFSAQKYDEKIAWLRQLLAWKNEVTDAVVDQEEVRRDWVEKLKSATLDERIY 420 Query: 421 VLTPQARVIELPSGATPIDFAYHLHSDVGHRCRGARVDGVMVPLNTALKNGQTVEIITVK 480 VLTPQARVIELPSGATPIDFAYHLHSDVGHRCRGARVDGVMVPLNTALKNGQTVEIITVK Sbjct: 421 VLTPQARVIELPSGATPIDFAYHLHSDVGHRCRGARVDGVMVPLNTALKNGQTVEIITVK 480 Query: 481 SGPGVGPSRDWLSPDYAVSSRTRSKIRAWFNAIEQQETLSVGRGLLEKTLQREGKTAVNL 540 SGPGVGPSRDWLSPDYAVSSRTRSKIRAWFNAIEQQETLSVGRGLLEKTLQREGKTAVNL Sbjct: 481 SGPGVGPSRDWLSPDYAVSSRTRSKIRAWFNAIEQQETLSVGRGLLEKTLQREGKTAVNL 540 Query: 541 EELAQKLGFAKVDDLCLSLAKEEFSLRHVEQALHEGQEKKPEIDDETLITRKSRASSIVQ 600 EELAQKLGFAKVDDLCLSLAKEEFSLRHVEQALHEGQEKKPEIDDETLITRKSRASSIVQ Sbjct: 541 EELAQKLGFAKVDDLCLSLAKEEFSLRHVEQALHEGQEKKPEIDDETLITRKSRASSIVQ 600 Query: 601 GAKSGVLVVGTDGLLTQLARCCKPAPPDAIAGFVTRGKGVSIHRVSCKNFAEMSSHAPER 660 GAKSGVLVVGTDGLLTQLARCCKPAPPDAIAGFVTRGKGVSIHRVSCKNFAEMSSHAPER Sbjct: 601 GAKSGVLVVGTDGLLTQLARCCKPAPPDAIAGFVTRGKGVSIHRVSCKNFAEMSSHAPER 660 Query: 661 VIQTTWGAPARETVYPVDIFVLASDRQGLLRDISDIFLRDKINVIGVSTQSVKGHARMAF 720 VIQTTWGAPARETVYPVDIFVLASDRQGLLRDISDIFLRDKINVIGVSTQSVKGHARMAF Sbjct: 661 VIQTTWGAPARETVYPVDIFVLASDRQGLLRDISDIFLRDKINVIGVSTQSVKGHARMAF 720 Query: 721 TAEIGSTEQLQKALTVIREVKGVIEAKRQ 749 TAEIGSTEQLQKALTVIREVKGVIEAKRQ Sbjct: 721 TAEIGSTEQLQKALTVIREVKGVIEAKRQ 749 >gi|300311444|ref|YP_003775536.1| bifunctional (P)ppGpp synthetase II/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase [Herbaspirillum seropedicae SmR1] Length = 753 Score = 365 bits (937), Expect = e-102, Method: Compositional matrix adjust. Identities = 234/742 (31%), Positives = 364/742 (49%), Gaps = 54/742 (7%) Query: 19 LGPEDAARVAQALAELEPLYADRQILSGQNALQFSISVATVLTALNVDAATRIAALAFEV 78 L P + +V +A + ++ + SG+ + ++VA + +DA +AA +V Sbjct: 53 LTPSELKKVKEAYRFADEMHLGQMRKSGEPYISHPLAVAEICAEWKLDAQAMMAAFLHDV 112 Query: 79 QMLHPELEEKLEERFGKEIADLVGNVRQLMRFHSFSFQPQQEVLRGKNAAQQAAAQIETL 138 +E+L ERFG +A LV + +L + FQ Q E AQ E Sbjct: 113 MEDQGVKKEELIERFGASVASLVDGLSKLDKI---EFQSQVE------------AQAENF 157 Query: 139 RKMLLAMATDMRAVLVRLASRVATLRYFAETKQEDEASANYARQTFELYAPLANRLGIWQ 198 RKMLLAMA D+R +LV+LA R+ +R E + +R+T E+Y P+A+RLG+ Sbjct: 158 RKMLLAMARDVRVILVKLADRLHNMRTLGSMPPEKKR--RISRETMEVYVPIAHRLGLNN 215 Query: 199 LKWELEDLSFRFLEPVTYKRIAKMLEEKRLEREAFVANAIARLRAEMAAQDIRAEVSGRP 258 + EL++LSF L P+ ++ ++K ++ R R V + + + + A I A+V GR Sbjct: 216 IYRELQELSFAHLHPLRHRTLSKAVKAARGNRREVVTKIMESVTSTLIAAGIPAQVDGRE 275 Query: 259 KHIYSIWNKMRGKHIDFSELYDVRAFRVIVDDVKTCYAVLGIVHDIWTPIPEEFDDYISR 318 K +Y I+ KMR KH+ FS++ DV FRV+VD CY LG +H ++ P+P +F DYI+ Sbjct: 276 KTLYGIYRKMRNKHLSFSQVLDVYGFRVVVDSFANCYVALGTLHSLFKPMPGKFKDYIAI 335 Query: 319 PKQNGYQSLHTVVTAEDGRALEVQIRTHEMHHFAEYGVAAHWRYKESGGSNFSAQKYDEK 378 PK NGYQSLHT + G +E QIRT EMH AE GVAAHW YK+ GS Q+ Sbjct: 336 PKLNGYQSLHTTLIGPYGTPVEFQIRTSEMHRVAESGVAAHWLYKDDEGSLTDLQQRTH- 394 Query: 379 IAWLRQLLAWKNEVTDAVVDQEEVRRDWVEKLKSATLDERIYVLTPQARVIELPSGATPI 438 AWL+ LL + + D+ +++E +K + +YV TP++++I LP GAT + Sbjct: 395 -AWLQSLLDIQKQTGDSA--------EFLEHVKVDLFPDSVYVFTPKSKIIALPRGATAL 445 Query: 439 DFAYHLHSDVGHRCRGARVDGVMVPLNTALKNGQTVEIITVKSGPGVGPSRDWLSPDYAV 498 DFAY +H+D+G + A ++ PL L NG VEIIT P PS +WL Y Sbjct: 446 DFAYSIHTDIGDQTTSAIINHEPAPLRAELHNGDIVEIIT---SPTSRPSPNWLG--YVR 500 Query: 499 SSRTRSKIRAWFNAIEQQETLSVGRGLLEKTLQREG----KTAVNLEELAQKLGFAKVDD 554 + + RS IR Q E+ +VGR LL L G + +E L + +DD Sbjct: 501 TGKARSAIRHRLRTANQVESQAVGRRLLGNALHTLGIEPELPSHIVERLLNESSAKTMDD 560 Query: 555 LCLSLA----KEEFSLRHVEQALHEGQEKKPEIDDETLITRKSRASSIVQGAKSGVLVVG 610 L + RH+ + G P++D E ++ V++ G Sbjct: 561 LYAEIGIGTRMAPLVARHIMTMMDTGV-SVPQLDPE---------GHMLPNKPDPVVITG 610 Query: 611 TDGLLTQLARCCKPAPPDAIAGFVTRGKGVSIHRVSCKNFAEMSSHAPERVIQTTWGAPA 670 ++G QL+ CC P P D + G++ + + +H C + P+R I+ WG Sbjct: 611 SEGASAQLSSCCMPIPGDRLTGYLKADQTLIVHTQECDTAKRLHEKEPDRWIELVWGQDL 670 Query: 671 RETVYPVDIFVLASDRQGLLRDISDIFLRDKINVIGVSTQSVKGHARMA---FTAEIGST 727 + I +L + +G L I+ N++ V+ G+A M FT ++ Sbjct: 671 NRR-FDCRITILIHNERGTLARIAAEIGESDANIVSVAMDDEGGNASMKYLRFTIQVEDR 729 Query: 728 EQLQKALTVIREVKGVIEAKRQ 749 L + + IR + V R+ Sbjct: 730 VHLARTMRGIRRIDSVARILRE 751 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,178,088 Number of extensions: 89813 Number of successful extensions: 298 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 294 Number of HSP's successfully gapped: 2 Length of query: 749 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 657 Effective length of database: 1,184,250 Effective search space: 778052250 Effective search space used: 778052250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)