BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0164.p (299 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311290|ref|YP_003775382.1| hydrolase or acyltransferase [H... 598 e-172 gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamil... 107 1e-24 gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum serop... 59 4e-10 >gi|300311290|ref|YP_003775382.1| hydrolase or acyltransferase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 598 bits (1541), Expect = e-172, Method: Compositional matrix adjust. Identities = 288/288 (100%), Positives = 288/288 (100%) Query: 12 MTEAVVKTVQCISPAGLHTMAYKEWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTYRV 71 MTEAVVKTVQCISPAGLHTMAYKEWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTYRV Sbjct: 1 MTEAVVKTVQCISPAGLHTMAYKEWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTYRV 60 Query: 72 ICPDVVGRGRSGRLANPQFYTVPQYVSDMVTLLARLDAQIVDWFGTSMGGLIGMALASLP 131 ICPDVVGRGRSGRLANPQFYTVPQYVSDMVTLLARLDAQIVDWFGTSMGGLIGMALASLP Sbjct: 61 ICPDVVGRGRSGRLANPQFYTVPQYVSDMVTLLARLDAQIVDWFGTSMGGLIGMALASLP 120 Query: 132 GNPIRRLVLNDIGPSINGAALARIGDYIGQDVRFDSFEEAAQYIRTISASFGQHSDEEWH 191 GNPIRRLVLNDIGPSINGAALARIGDYIGQDVRFDSFEEAAQYIRTISASFGQHSDEEWH Sbjct: 121 GNPIRRLVLNDIGPSINGAALARIGDYIGQDVRFDSFEEAAQYIRTISASFGQHSDEEWH 180 Query: 192 KLAKDVLRQNEQGQWVRHYDLALALPFKQTTPEAASAAEQMLWAAYDAIRCPTLLVRGAQ 251 KLAKDVLRQNEQGQWVRHYDLALALPFKQTTPEAASAAEQMLWAAYDAIRCPTLLVRGAQ Sbjct: 181 KLAKDVLRQNEQGQWVRHYDLALALPFKQTTPEAASAAEQMLWAAYDAIRCPTLLVRGAQ 240 Query: 252 SDLLLPEVAQEMTRRGPKAKLVELPDVGHAPTFMHAPQIEVARQFLLG 299 SDLLLPEVAQEMTRRGPKAKLVELPDVGHAPTFMHAPQIEVARQFLLG Sbjct: 241 SDLLLPEVAQEMTRRGPKAKLVELPDVGHAPTFMHAPQIEVARQFLLG 288 >gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 289 Score = 107 bits (266), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 84/270 (31%), Positives = 135/270 (50%), Gaps = 18/270 (6%) Query: 35 EWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTYRVICPDVVGRGRSGRLANPQFYTVP 94 EWGD + +L+ +HG+ + F +A+ L YR I D GRG+S A +YT Sbjct: 19 EWGDPQGFPLLL-LHGIRGYGETFGDLAQRLPPGYRCIALDQRGRGQSDWDAGANYYT-D 76 Query: 95 QYVSDMVTLLARLDAQIVDWFGTSMGGLIGMALASLPGNPIRRLVLNDIGPSI--NGAAL 152 YV+D+ L+ARL+ + D G SMGG+ + A+ +RRL++ D GP N Sbjct: 77 AYVADVEALVARLNLRQFDLLGHSMGGINAIVYAARHPAKVRRLIIEDAGPGAFENSPGA 136 Query: 153 ARIGDYIGQD-VRFDSFEEAAQYIRTISASFGQHSDEEWHKLAKDVLRQNEQGQWVRHYD 211 RI + F S++EA+ ++R + S ++E + ++L+ QG + YD Sbjct: 137 TRIRRELATTPSHFASWDEASTFMRALRPSV---TEEARQQRLHNMLKPAAQGGYTWRYD 193 Query: 212 LALALPFKQTTPEAASAAEQMLWAAYDAIRCPTLLVRGAQSDLLLPEVAQEMTRRGPKAK 271 + + + +P+ + + L A+ CPTL+VRG +SD L E+A EM + + Sbjct: 194 -HVGISSTRLSPDLSRVVD--LRPHIMALMCPTLVVRGERSDYLSAEMAAEMRQLNARIL 250 Query: 272 LVELPDVGHAPTFMHAPQ----IEVARQFL 297 + D GH ++H Q EV RQFL Sbjct: 251 TSTIADAGH---YIHDDQPEAFAEVVRQFL 277 >gi|300312579|ref|YP_003776671.1| hydrolase [Herbaspirillum seropedicae SmR1] Length = 300 Score = 59.3 bits (142), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 75/283 (26%), Positives = 122/283 (43%), Gaps = 34/283 (12%) Query: 34 KEWGDARNPNVLVCVHGVTRVSDDFDRMARELCDTYRVICPDVVGRGRSGRLANPQFYTV 93 + WGD P + + +HG +S F M L + VI PD G G + R + Y Sbjct: 25 RHWGDPEAPKIFM-LHGWMDMSASFQFMVDALQGQWHVIAPDWRGFGLTER-SQADSYWF 82 Query: 94 PQYVSDMVTLLARL-DAQIVDWFGTSMGGLIGMALASLPGNPIRRLV-LNDIG-PSIN-G 149 P Y++D+ ++ ++ ++ G SMGG + A + I+RL+ L +G P+ Sbjct: 83 PDYLADLDAIIDHYAGSEAINLLGHSMGGNVVTLYAGVRPQRIKRLINLEGLGLPATQPE 142 Query: 150 AALARIGDYIGQDVR-------FDSFEEAAQYIRTISASFGQHSDEEWHKLAKDVLRQNE 202 A R ++ +VR + + EE AQ ++ +A SDE LA+ +Q++ Sbjct: 143 QAPGRFAQWM-DEVRNRPVLRGYATLEEVAQRLQRNNARL---SDERAAFLAQHWAQQDD 198 Query: 203 QGQWVRHYDLALALPFKQTTPEAASAAEQMLWAAYDAIRCPTLLVRGAQSDLL-----LP 257 G W D A KQ +P A E M A + I P L + SD+ Sbjct: 199 SGLWQILGDPA----HKQVSPILYRADEMM--ACWRRITAPVLWIEAIDSDIWRFFGPAS 252 Query: 258 EVAQEMTRR---GPKAKLVELPDVGHAPTFMHAPQIEVARQFL 297 +E+ RR P+ ++ + D GH +H Q E Q + Sbjct: 253 LKREEIDRRIACIPRVQVEMIADAGH---MLHHDQPEQLAQLI 292 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 992,360 Number of extensions: 42203 Number of successful extensions: 164 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 3 Length of query: 299 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 214 Effective length of database: 1,217,395 Effective search space: 260522530 Effective search space used: 260522530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)