BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0141.p (477 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 846 0.0 gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 263 2e-71 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 262 5e-71 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 252 5e-68 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 236 3e-63 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 846 bits (2186), Expect = 0.0, Method: Compositional matrix adjust. Identities = 465/477 (97%), Positives = 468/477 (98%) Query: 1 MSRHLRLLPTLALCACAPLTAPLPPSALALPTHYPRTGAADAQANAAPSASAIGWQDYFP 60 MSRHLRLLPTLALCACAPLTAPLPPSAL LPTHYPRT A DA ANAAP+ASAIGWQDYFP Sbjct: 5 MSRHLRLLPTLALCACAPLTAPLPPSALDLPTHYPRTLAEDAPANAAPTASAIGWQDYFP 64 Query: 61 QAPLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPPE 120 QAPLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPPE Sbjct: 65 QAPLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPPE 124 Query: 121 SGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAAW 180 SGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAAW Sbjct: 125 SGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAAW 184 Query: 181 TSLRELDERLHLLESLQAATAESLALMQARHDAGRADAGALAQQRSELAQMRAEQAELQQ 240 TSLRELDERLHLLE+LQAATAESLALMQARHDAGRADA ALAQQRSELAQMRAEQAELQQ Sbjct: 185 TSLRELDERLHLLETLQAATAESLALMQARHDAGRADAAALAQQRSELAQMRAEQAELQQ 244 Query: 241 MRASQRSMLEMLAGLMQLPAALEPAPTTLLLPVALAPGLPSTLLVARPDIIAAEHRLQAS 300 MRASQRS LEMLAGL QLPAALEPAPTT+LLP ALAPGLPSTLL ARPDIIAAEHRLQAS Sbjct: 245 MRASQRSTLEMLAGLTQLPAALEPAPTTILLPFALAPGLPSTLLAARPDIIAAEHRLQAS 304 Query: 301 AAQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQSRYD 360 AAQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQSRYD Sbjct: 305 AAQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQSRYD 364 Query: 361 IAALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMHMRLR 420 IAALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMHMRLR Sbjct: 365 IAALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMHMRLR 424 Query: 421 YGRASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGGTQALSASSPSQQE 477 YGRASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGGTQALSASSPSQQE Sbjct: 425 YGRASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGGTQALSASSPSQQE 481 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 263 bits (672), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 184/443 (41%), Positives = 247/443 (55%), Gaps = 4/443 (0%) Query: 22 PLPPSALALPTHYPRTGAADAQANAAPSASAIGWQDYFPQAPLQALIAEALRHNPDLRDI 81 P P A P P AA AN A S + W+DYF PLQALI +AL ++ DLR Sbjct: 35 PAAPVAAVYPDG-PVNEAAHQVANEAASPP-LPWRDYFTDPPLQALITQALENSRDLRTA 92 Query: 82 RLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPPESGPAASTREYRVGLGASSWEL 141 LR+Q+ QA + I + PT+ +A RSR P + + Y+ GLG SSWE+ Sbjct: 93 ALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQAGLGLSSWEI 152 Query: 142 DFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAAWTSLRELDERLHLLESLQAATA 201 D WGR+ SL++AAL+ AS+A A+ +SL+ QVA AW L ELDERL + A+ Sbjct: 153 DLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLEIANQTIASRQ 212 Query: 202 ESLALMQARHDAGRADAGALAQQRSELAQMRAEQAELQQMRASQRSMLEMLAGL-MQLPA 260 ES + R + G L Q + L Q +A A+LQQ RA + + L +L G + LP Sbjct: 213 ESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTLLVGSPVVLPV 272 Query: 261 ALEPAPTTLLLPVALAPGLPSTLLVARPDIIAAEHRLQASAAQVAAARAAFFPRISLTAF 320 A + L PGLP+ LL RPD+ AAEHRL A+ A + AARAAFFP ISLT Sbjct: 273 GQARAGLDAVF-APLQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAAFFPSISLTGN 331 Query: 321 GGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQSRYDIAALDQQRALLDYERTVQNA 380 G AS L LF+ S AW F P I+LP+ G ++ ++ + + A+ YE+TVQ A Sbjct: 332 VGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAAYEKTVQTA 391 Query: 381 FREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMHMRLRYGRASRLDVLESERDHLRAQ 440 FREVA L WL +QL + + V+ Q +R +R G A LDVL+++RD L AQ Sbjct: 392 FREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLDAQRDLLSAQ 451 Query: 441 QRQHMLEHALLRNQIALYVALGG 463 Q+ + ALL + +ALY ALGG Sbjct: 452 QQLVQIRRALLSSSVALYAALGG 474 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 262 bits (669), Expect = 5e-71, Method: Compositional matrix adjust. Identities = 174/483 (36%), Positives = 258/483 (53%), Gaps = 21/483 (4%) Query: 1 MSRHLRLLPTLALCACAPLTA-PLPPSALALPTHYPRTGAADAQANAAPSASAIGWQDYF 59 M L + L C+ AP P P + + YP ADA A A +GW+ +F Sbjct: 15 MCAALVMAGVLGGCSLAPTYERPQAP----VESSYP----ADAAGRAELQAVNLGWRQFF 66 Query: 60 PQAPLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPP 119 P LQ LIA AL +N DLR L +++ +AQ++I R+ LP +NL A R+R Sbjct: 67 PDQRLQQLIAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQS 126 Query: 120 ESGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAA 179 +G + Y+VGL ++ELDF+GR+ +L +AAL ++++ A+++ ISL+ QVA A Sbjct: 127 LTGQPTISNSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQA 186 Query: 180 WTSLRELDERLHLLESLQAATAESLALMQARHDAGRADAGALAQQRSELAQMRAEQAELQ 239 + + R E+L L + + +L L Q R D G + A ++ + R + +L+ Sbjct: 187 YLAERSYAEQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQLE 246 Query: 240 QMRASQRSMLEMLAG--LMQLPAALEPAPTTLL----LPVALAPGLPSTLLVARPDIIAA 293 + RA + L +L G + +LP P LL + + GLPS LL RPDI +A Sbjct: 247 RQRAQAENALTLLVGKKIGELP------PAQLLSDEKIVTDIPAGLPSDLLTNRPDIRSA 300 Query: 294 EHRLQASAAQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGG 353 E L A+ A + AARAAFFPRISLT G AS+ L LF+ S AW+F P +SLP+ D G Sbjct: 301 EQSLLAANANIGAARAAFFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFG 360 Query: 354 LRQSRYDIAALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQA 413 + D+A + + +A++ YE+ +Q AFREVA L L EQ++ + + Q +R Sbjct: 361 RNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLK 420 Query: 414 QMHMRLRYGRASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGGTQALSASSP 473 +R + G AS LD L+++RD AQQ L N I LY ALGGG + S Sbjct: 421 LTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAIDLYKALGGGLNENTVSVA 480 Query: 474 SQQ 476 ++Q Sbjct: 481 AKQ 483 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 252 bits (643), Expect = 5e-68, Method: Compositional matrix adjust. Identities = 161/429 (37%), Positives = 236/429 (55%), Gaps = 7/429 (1%) Query: 42 AQANAAPSASAIGWQDYFPQAPLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLP 101 A A++AP + IGW+D F LQ +IA AL +N DLR L +++ +AQ+ +QR+ P Sbjct: 53 AGASSAPVVADIGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFP 112 Query: 102 TVNLEAQARRSRGPLEPPESGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMAS 161 ++ SR P + +G A ++R Y LG S++ELD +GR+ SL E ALQ ++++ Sbjct: 113 SIKAATSETASRTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLST 172 Query: 162 RAAEQALVISLIGQVAAAWTSLRELDERLHLLESLQAATAESLALMQARHDAGRADAGAL 221 A ++ ISLI +VA AW +L +RL L + + + S AL Q + G + A L Sbjct: 173 AEARRSTQISLIAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTL 232 Query: 222 AQQRSELAQMRAEQAELQQMRASQRSMLEMLAGLMQLPAALEPA--PTTLLL---PVA-L 275 Q ++ + R + R+ L +L G LP AL P P T L P+A + Sbjct: 233 RQAQTSVESARVDVESYTAQVDQDRNALVLLVG-KDLPTALLPQGLPDTRLAAASPLATI 291 Query: 276 APGLPSTLLVARPDIIAAEHRLQASAAQVAAARAAFFPRISLTAFGGVASTALQDLFKGP 335 PGLPS LL R DI+ AE L+A+ A + AARAAF+P I+LT G AS L LFKG Sbjct: 292 PPGLPSDLLQRRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGG 351 Query: 336 SRAWNFVPEISLPLLDGGLRQSRYDIAALDQQRALLDYERTVQNAFREVAQRLEEAHWLK 395 S +W+FVP+ISLP+ DGG Q+ DIA + + ++ YE+ +Q AFREV+ L + + L Sbjct: 352 SGSWSFVPQISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLG 411 Query: 396 EQLRLRREVVQQQAQRQAQMHMRLRYGRASRLDVLESERDHLRAQQRQHMLEHALLRNQI 455 QL+ + +VQ + R G S L VL+S+R AQ+ + + N + Sbjct: 412 RQLQAQEALVQASDEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLV 471 Query: 456 ALYVALGGG 464 Y LGGG Sbjct: 472 TFYKTLGGG 480 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 236 bits (602), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 158/446 (35%), Positives = 235/446 (52%), Gaps = 4/446 (0%) Query: 21 APLPPSALALPTHYPRTGAADAQANAAPSASAIGWQDYFPQAPLQALIAEALRHNPDLRD 80 AP+P S + P G+A A A +GW+++F LQ +IA AL +N DLR Sbjct: 40 APVPASWTSQAAAQPAVGSAGPAAGEI-DADHLGWREFFLDPRLQQVIAAALDNNRDLRV 98 Query: 81 IRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEPPESGPAASTREYRVGLGASSWE 140 L +++ +AQ+RI + P+++ + SR P E +G AA + + V +G S++E Sbjct: 99 AALNIEKARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAVSHQQSVTVGVSAYE 158 Query: 141 LDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAAAWTSLRELDERLHLLESLQAAT 200 LDF+GR+ +L++AAL+ + ++ ISL+ +VA AW +L + L L + A+ Sbjct: 159 LDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRDLLKLAQDTYASQ 218 Query: 201 AESLALMQARHDAGRADAGALAQQRSELAQMRAEQAELQQMRASQRSMLEMLAGLMQLPA 260 + + AL Q HD G LA Q + + RA+ A + L +L G + A Sbjct: 219 SRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNALRLLVG-ADIDA 277 Query: 261 ALEPA--PTTLLLPVALAPGLPSTLLVARPDIIAAEHRLQASAAQVAAARAAFFPRISLT 318 AL PA P T + A GLPS +L+ RPD++AAEH L+++ A + AARAAFFP ISLT Sbjct: 278 ALLPAGLPDTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLT 337 Query: 319 AFGGVASTALQDLFKGPSRAWNFVPEISLPLLDGGLRQSRYDIAALDQQRALLDYERTVQ 378 A GG AS L LFK S +W F P I+LP+ + G ++ D A + + + +YE+ +Q Sbjct: 338 ANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITRDIDVANYEKAIQ 397 Query: 379 NAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQAQMHMRLRYGRASRLDVLESERDHLR 438 AFREVA L L ++L + + + R R G S L L ++R Sbjct: 398 TAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSYLTTLVAQRTLYS 457 Query: 439 AQQRQHMLEHALLRNQIALYVALGGG 464 AQQ L N+I LY LGGG Sbjct: 458 AQQTLISLRLTEQSNRITLYKVLGGG 483 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.129 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,053,727 Number of extensions: 35574 Number of successful extensions: 239 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 193 Number of HSP's successfully gapped: 18 Length of query: 477 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 388 Effective length of database: 1,198,455 Effective search space: 465000540 Effective search space used: 465000540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)