BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0126.p (492 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine... 966 0.0 gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine... 407 e-115 gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine... 234 1e-62 gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirill... 89 1e-18 >gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 492 Score = 966 bits (2496), Expect = 0.0, Method: Compositional matrix adjust. Identities = 490/492 (99%), Positives = 491/492 (99%) Query: 1 MTPLKTTLASALLAASVFVAAPVALAAPPVASPAVAALPDFADIVERTGPGVVNIRTTER 60 MTPLKTTLASALLAASVFVAAPVALAAPPVASPAVAALPDFADIVERTGPGVVNIRTTER Sbjct: 1 MTPLKTTLASALLAASVFVAAPVALAAPPVASPAVAALPDFADIVERTGPGVVNIRTTER 60 Query: 61 VRQNAQGQGGVDDPEMQEFFRRFFGIPIPRQQQPGTPRGNGKQGGQGQQEEVPRGVGSGF 120 VRQNAQGQGGVDDPEMQEFFRRFFGIPIPRQQQPGTPRGNGKQGGQGQQEEVPRGVGSGF Sbjct: 61 VRQNAQGQGGVDDPEMQEFFRRFFGIPIPRQQQPGTPRGNGKQGGQGQQEEVPRGVGSGF 120 Query: 121 IISADGFIMTNAHVVEGASEVYVTLTDKREFKAKIVGSDTRTDVAVLKIDGSNLPRLNMG 180 IISADGFIMTNAHVVEGASEVYVTLTDKREFKAKIVGSDTRTDVAVLKIDGSNLPRLNMG Sbjct: 121 IISADGFIMTNAHVVEGASEVYVTLTDKREFKAKIVGSDTRTDVAVLKIDGSNLPRLNMG 180 Query: 181 DSDKIRVGEWVLAIGSPFGLENTVTAGIVSAKARDTGDYLPLIQTDVAVNPGNSGGPLIN 240 DSDKIRVGEWVLAIGSPFGLENTVTAGIVSAKARDTGDYLPLIQTDVAVNPGNSGGPLIN Sbjct: 181 DSDKIRVGEWVLAIGSPFGLENTVTAGIVSAKARDTGDYLPLIQTDVAVNPGNSGGPLIN 240 Query: 241 LKGEVIGINSQIYSRSGGFMGISFAVPIDEALRVADQLKASGRVTRGRIGVQIGEVTKDV 300 LKGEVIGINSQIYSRSGGFMGISFAVPIDEALRVADQLKASGRVTRGRIGVQIGEVTKDV Sbjct: 241 LKGEVIGINSQIYSRSGGFMGISFAVPIDEALRVADQLKASGRVTRGRIGVQIGEVTKDV 300 Query: 301 AESLGLARAQGALVQRVEAGGPAEKAGLEAGDIILKYNGAAIERPSDLPRMVGSTKPGAK 360 AESLGLARAQGALVQRVEAGGPAEKAGLEAGDIILKYNGAAIERPSDLPRMVGSTKPGAK Sbjct: 301 AESLGLARAQGALVQRVEAGGPAEKAGLEAGDIILKYNGAAIERPSDLPRMVGSTKPGAK 360 Query: 361 ATVSIWRKGNARDVSVTVVELEADKPKQAEEKKAKPGNAPNSLGLVVSDLSDAQRKELKV 420 ATVSIWRKG+ARDVSVTVVELEADKPKQAEEKKAKP NAPNSLGLVVSDLSDAQRKELKV Sbjct: 361 ATVSIWRKGSARDVSVTVVELEADKPKQAEEKKAKPDNAPNSLGLVVSDLSDAQRKELKV 420 Query: 421 DNGVLVEAVSGAAAAAGIRPGDVIQRLNEVDVKDAKQFAQLVSKLDAKKRAAVLVRRGDS 480 DNGVLVEAVSGAAAAAGIRPGDVIQRLNEVDVKDAKQFAQLVSKLDAKKRAAVLVRRGDS Sbjct: 421 DNGVLVEAVSGAAAAAGIRPGDVIQRLNEVDVKDAKQFAQLVSKLDAKKRAAVLVRRGDS 480 Query: 481 SQFVPLRPNGGN 492 SQFVPLRPNGGN Sbjct: 481 SQFVPLRPNGGN 492 >gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine endoprotease [Herbaspirillum seropedicae SmR1] Length = 494 Score = 407 bits (1047), Expect = e-115, Method: Compositional matrix adjust. Identities = 223/454 (49%), Positives = 297/454 (65%), Gaps = 27/454 (5%) Query: 40 DFADIVERTGPGVVNIRTTERVRQNAQGQGGVDDPE--MQEFFRRFFGIPIPRQQQPGTP 97 DF+ IV+R GP VVNI T + R+ A DP EFF+RF P+ Q P P Sbjct: 64 DFSGIVQRYGPAVVNISVTGKARKVADDDDNGLDPNDPFSEFFKRFG----PQLQMPQQP 119 Query: 98 RGNGKQGGQGQQEEVPRGVGSGFIISADGFIMTNAHVVEGASEVYVTLTDKREFKAKIVG 157 R + G+GSGFIIS DG I+TNAHVV+GA EV V LTD+REFKAK++G Sbjct: 120 R-------------IMHGLGSGFIISPDGLILTNAHVVDGAQEVVVKLTDRREFKAKVLG 166 Query: 158 SDTRTDVAVLKIDGSNLPRLNMGDSDKIRVGEWVLAIGSPFGLENTVTAGIVSAKARDTG 217 D ++D+AV++ID NLP + +GD +++VG+ VLAIGSP+G +NT TAGI+SAK+R Sbjct: 167 IDKQSDIAVIRIDAKNLPTVQIGDPSRVKVGQPVLAIGSPYGFDNTATAGIISAKSRSLP 226 Query: 218 D--YLPLIQTDVAVNPGNSGGPLINLKGEVIGINSQIYSRSGGFMGISFAVPIDEALRVA 275 D Y+P IQTDVAVNPGNSGGPL +L G+VIGINSQIYS++GGF G+SF++PID A++V Sbjct: 227 DDNYVPFIQTDVAVNPGNSGGPLFDLNGQVIGINSQIYSQTGGFQGLSFSIPIDVAMKVE 286 Query: 276 DQLKASGRVTRGRIGVQIGEVTKDVAESLGLARAQGALVQRVEAGGPAEKAGLEAGDIIL 335 QL G+VTRGR+GV + ++ + +++S GL +A+GALV VE G PA+KAGL+AGD+I+ Sbjct: 287 QQLVTHGKVTRGRLGVSVQDLNQALSDSFGLKKAEGALVSSVEKGSPADKAGLQAGDVII 346 Query: 336 KYNGAAIERPSDLPRMVGSTKPGAKATVSIWRKGNARDVSVTVVELEADKPKQAEEKKAK 395 +NG AI+ DLP +V T PG+ + + R G R ++VTV E++ K + KA Sbjct: 347 SFNGHAIDHSVDLPTLVADTPPGSTKPMEVMRAGKVRTINVTVGEMKQAK----NDAKAS 402 Query: 396 PGNAPNSLGLVVSDLSDAQRKELKVDNGVLVEAVSGAAAAAGIRPGDVIQRLNEVDVKDA 455 + LGL V L ++ +L G+LVE VSG AA AGI+ GDVI +N V Sbjct: 403 KSDDQGRLGLAVRPLDKDEQAQLGGQKGLLVEDVSGPAAVAGIQSGDVILAVNGTPVSSV 462 Query: 456 KQFAQLVSKLDAKKRAAVLVRRGDSSQFVPLRPN 489 Q +L + DA K A+LV RGD FVPL N Sbjct: 463 AQLRKLAT--DAGKNVALLVERGDEKIFVPLALN 494 >gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 393 Score = 234 bits (598), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 139/352 (39%), Positives = 207/352 (58%), Gaps = 27/352 (7%) Query: 31 ASPAVAALPDFADIVERTGPGVVNIRTTERVR-QNAQGQGGVDDPEMQEFFRRFFGIPIP 89 A+P AL + + P VVNI TT R Q + Q +DP FFR+FFG Sbjct: 50 ATPNAPALDSYRHAARQAMPSVVNIFTTTEARPQKSPFQ---NDP----FFRKFFG---- 98 Query: 90 RQQQPGTPRGNGKQGGQGQQEEVPRGVGSGFIISADGFIMTNAHVVEGASEVYVTLTDKR 149 Q + QQ++ +GSG I+S G+I+TN HVVE A ++ V L D R Sbjct: 99 ------------DQFDEQQQDDKQSSLGSGVIVSPQGYILTNNHVVEAADKIEVALADGR 146 Query: 150 EFKAKIVGSDTRTDVAVLKIDGSNLPRLNMGDSDKIRVGEWVLAIGSPFGLENTVTAGIV 209 + AK+VG D TD+AV+KID NLP + +G + VG+ VLAIG+PFG+ TVT GIV Sbjct: 147 KASAKVVGIDPETDLAVIKIDLPNLPAITLGHPENSSVGDVVLAIGNPFGVGQTVTMGIV 206 Query: 210 SAKARD---TGDYLPLIQTDVAVNPGNSGGPLINLKGEVIGINSQIYSRSGGFMGISFAV 266 SA R+ + IQTD A+NPGNSGG L++ G ++GIN+ IYSR+GG +GI FA+ Sbjct: 207 SALGRNHLGINTFENFIQTDAAINPGNSGGALVDTNGNLLGINTAIYSRTGGNLGIGFAI 266 Query: 267 PIDEALRVADQLKASGRVTRGRIGVQIGEVTKDVAESLGLARAQGALVQRVEAGGPAEKA 326 P+ A V + + + G+V RG IGV+ ++T ++AES GL + GA++ V GGPA++A Sbjct: 267 PMSTAKTVMEAIISHGQVVRGWIGVEPQDITPELAESFGLGKKTGAIIAGVLKGGPADRA 326 Query: 327 GLEAGDIILKYNGAAIERPSDLPRMVGSTKPGAKATVSIWRKGNARDVSVTV 378 G+ GDI++ + + +++ ++ PG + +++ RK +++TV Sbjct: 327 GMRPGDILVSIDDKPVADTTEMLNVIAQLTPGQQIAMTVLRKSQETKLNITV 378 >gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirillum seropedicae SmR1] Length = 438 Score = 88.6 bits (218), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 62/167 (37%), Positives = 97/167 (58%), Gaps = 9/167 (5%) Query: 117 GSGFIISADGFIMTNAHVVEGASEVYVTLTDKREFKAKIVGSDTRTDVAVLKIDGSNLPR 176 G+GF I G++++N HVV A V V L RE A+++ D DVA+LK + + Sbjct: 271 GTGFYIGQSGWLLSNQHVVGTARSVRVRLPGGRELPAEVLRVDAARDVALLKTEPPGVRP 330 Query: 177 LNMGDSDKIRVGEWVLAIGSPFG--LENTVTAGIVSAKARDTGDYLPLIQTDVAVNPGNS 234 L + D+ +GE V A+GSP G T+T GI+S G +Q+DVA+ PG+S Sbjct: 331 LPL-RLDEPGLGEDVYALGSPLGDTFNTTLTRGILSGVRLLNGQE--FLQSDVAILPGSS 387 Query: 235 GGPLINLKGEVIGIN-SQIYSRSGGFMGISFAVPIDEAL-RVADQLK 279 GGPL++ G+V+GI + + +R G G++F +P+ A+ R+ QL+ Sbjct: 388 GGPLLDRSGQVVGITVAGLGAR--GMAGMNFFIPLASAVERLNLQLQ 432 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.134 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,288,441 Number of extensions: 50928 Number of successful extensions: 165 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 155 Number of HSP's successfully gapped: 4 Length of query: 492 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 403 Effective length of database: 1,198,455 Effective search space: 482977365 Effective search space used: 482977365 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)