BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0107.p (585 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311237|ref|YP_003775329.1| GGDEF domain-containing protein... 1160 0.0 gi|300310067|ref|YP_003774159.1| diguanylate cyclase GGDEF famil... 127 2e-30 gi|300313424|ref|YP_003777516.1| GGDEF domain-containing protein... 125 1e-29 gi|300310347|ref|YP_003774439.1| GGDEF domain transmembrane prot... 123 4e-29 gi|300312078|ref|YP_003776170.1| hypothetical protein Hsero_2767... 122 8e-29 >gi|300311237|ref|YP_003775329.1| GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 585 Score = 1160 bits (3002), Expect = 0.0, Method: Compositional matrix adjust. Identities = 579/583 (99%), Positives = 581/583 (99%) Query: 1 MSLNRLFRLISIALLLLFLAFAVSLVWTEWNSYRNGANSVPALRTFRLALVALEKLSAER 60 MSLNRLFRLISIALLLLFLAFAVSLVWTEWNSYRNGANSVPALRTFRLALVALEKLSAER Sbjct: 1 MSLNRLFRLISIALLLLFLAFAVSLVWTEWNSYRNGANSVPALRTFRLALVALEKLSAER 60 Query: 61 GPANAYMGASASQREAVADKLKNARAETDAALAALRQALQSHARLRQSWSLDKVKTVQQA 120 GPANA+MGASASQREAVADKLKNARAETDAALAALRQALQSH RLRQSWSLDKVKTVQQA Sbjct: 61 GPANAFMGASASQREAVADKLKNARAETDAALAALRQALQSHPRLRQSWSLDKVKTVQQA 120 Query: 121 LEPARAAIDRIGRQPEAERNNVYVLSAVGGMVDMIPLMIRVASELSSEVDHADPELREGL 180 LEPARAAIDRIGRQPEAERNNVYVLSAVGGMVDMIPLMIRVASELSSEVDHADPELREGL Sbjct: 121 LEPARAAIDRIGRQPEAERNNVYVLSAVGGMVDMIPLMIRVASELSSEVDHADPELREGL 180 Query: 181 AAIRAAASLREYAGQVGSRFTPALIAHRKLTQDEVIEIGRLYGRIELLRGLLNTQMVSYV 240 AAIRAAASLREYAGQVGSRFTPALIAHRKLTQDEVIEIGRLYGRIELLRGLLNTQM SYV Sbjct: 181 AAIRAAASLREYAGQVGSRFTPALIAHRKLTQDEVIEIGRLYGRIELLRGLLNTQMASYV 240 Query: 241 QRPEFARALELVDKQYFDNGFQFLDFLLQTGVASGDYGLTSVDLFRFYVPQMKSITDLRD 300 QRPEFARALELVDKQYFDNGFQFLDFLLQTGVASGDYGLTSVDLFRFYVPQMKSITDLRD Sbjct: 241 QRPEFARALELVDKQYFDNGFQFLDFLLQTGVASGDYGLTSVDLFRFYVPQMKSITDLRD 300 Query: 301 LLLDDMMEQAERHNRRARNYLLLVIAVTVVACLFFQVLMVLIRRRVVQPLVLATDLVSGL 360 LLLDDMMEQAERHNRRARNYLLLVIAVTVVACLFFQVLMVLIRRRVVQPLVLATDLVSGL Sbjct: 301 LLLDDMMEQAERHNRRARNYLLLVIAVTVVACLFFQVLMVLIRRRVVQPLVLATDLVSGL 360 Query: 361 VEGRLDQEIPQPRYEDEISAMMRALKVLRQRMRERNSLAREREALITQLQTSSNTDFLTG 420 VEGRLDQEIPQPRYEDEISAMMRALKVLRQRMRERNSLAREREALITQLQTSSNTDFLTG Sbjct: 361 VEGRLDQEIPQPRYEDEISAMMRALKVLRQRMRERNSLAREREALITQLQTSSNTDFLTG 420 Query: 421 VLNRRAFFTHGQQQMTVAKRYGRELAVVLFDIDHFKQINDSYGHQAGDAILRGVAQSVSQ 480 VLNRRAFFTHGQQQMTVAKRYGRELAVVLFDIDHFKQINDSYGHQAGDAILRGVAQSVSQ Sbjct: 421 VLNRRAFFTHGQQQMTVAKRYGRELAVVLFDIDHFKQINDSYGHQAGDAILRGVAQSVSQ 480 Query: 481 LLRKVDVLARYGGEEFIILLPESSLLQAAAASEKLRAALAAGEFEIDGGRRIRVTASFGV 540 LLRKVDVLARYGGEEFIILLPESSLLQAAAASEKLRAALAAGEFEIDGGRRIRVTASFGV Sbjct: 481 LLRKVDVLARYGGEEFIILLPESSLLQAAAASEKLRAALAAGEFEIDGGRRIRVTASFGV 540 Query: 541 AAENGGPGLEHLIKQADIALYRAKKLGRNRVKLAGEEEGVEGA 583 AAENGGPGLEHLIKQADIALYRAKKLGRNRV+LAGEEEGVEGA Sbjct: 541 AAENGGPGLEHLIKQADIALYRAKKLGRNRVELAGEEEGVEGA 583 >gi|300310067|ref|YP_003774159.1| diguanylate cyclase GGDEF family protein [Herbaspirillum seropedicae SmR1] Length = 369 Score = 127 bits (319), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 102/296 (34%), Positives = 150/296 (50%), Gaps = 36/296 (12%) Query: 317 ARNYLLLVIAVTVVACLFFQVLMVLIRRRVVQPLVLATDLVSGLVEGRLD--QEIPQP-- 372 A + L++I + + + + + + RR + QP+ D V L +++ + I P Sbjct: 69 AYGFTLILINSVIKTLVLWFIFLYVFRRWLGQPITRLADFVRELDLNQMETPRSIHLPGR 128 Query: 373 -RYE-----DEISAMMRAL-----------KVLRQRMRERNSLAREREALIT-------- 407 R+E D I+ M+ L K L Q + ++L R E I Sbjct: 129 RRHELHFLADAINLMLAKLHRHTIHNHALYKELEQEKQSLSALNRSLELRIAERTRDLAQ 188 Query: 408 ---QLQTSSNTDFLTGVLNRRAFFTHGQQQMTVAKRYGRELAVVLFDIDHFKQINDSYGH 464 QL++ S TD LTG+ NRR+F +Q+ + R+G L + D+D FK ND YGH Sbjct: 189 ANEQLRSLSLTDGLTGIANRRSFDETLEQEWRRSMRHGTPLMLAFIDVDWFKPYNDHYGH 248 Query: 465 QAGDAILRGVAQSVSQLLRKV-DVLARYGGEEFIILLPESSLLQAAAASEKLRAALAAGE 523 AGDA+LR VAQ++ Q + +V D +ARYGGEEF I+ ++ A ++ A+ A Sbjct: 249 LAGDAVLRQVAQTLRQSIARVGDTVARYGGEEFAIIAADTDGQAGADMLRRMCDAVHALA 308 Query: 524 FEIDGGRRIRVTASFGVAAEN---GGPGLEHLIKQADIALYRAKKLGRNRVKLAGE 576 G R+T S GVA+ N G GL+ L++ AD ALYRAK GRNRV LA E Sbjct: 309 IAHAGSAMGRITISAGVASCNPGQPGAGLDGLLQAADAALYRAKMEGRNRVVLATE 364 >gi|300313424|ref|YP_003777516.1| GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 372 Score = 125 bits (314), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 72/169 (42%), Positives = 105/169 (62%), Gaps = 8/169 (4%) Query: 415 TDFLTGVLNRRAFFTHGQQQMTVAKRYGRELAVVLFDIDHFKQINDSYGHQAGDAILRGV 474 TD LTGV NRR Q+++ ++R L+ + DIDHFK++ND +GHQ+GD +LR V Sbjct: 202 TDPLTGVNNRRYIEQRMQEEVARSQRDRSALSCLFIDIDHFKRVNDQFGHQSGDDVLREV 261 Query: 475 AQSVSQLLRKVDVLARYGGEEFIILLPESSLLQAAAASEKLRAALAAGEFEIDGGRR-IR 533 A + + LR D L R+GGEEF++LL ++ AA +E++RA + + I+G R ++ Sbjct: 262 AARIKKELRLSDALGRFGGEEFVVLLTHATREDAARIAERIRAGVCSRGVMIEGSRDPLK 321 Query: 534 VTASFGVAA----ENGGPGLE---HLIKQADIALYRAKKLGRNRVKLAG 575 +TAS G AA + P E ++K AD ALY+AK+ GRNRV LA Sbjct: 322 ITASIGAAALTPPDRSRPAAEVKQAMLKAADQALYQAKEAGRNRVMLAA 370 >gi|300310347|ref|YP_003774439.1| GGDEF domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 334 Score = 123 bits (308), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 79/182 (43%), Positives = 111/182 (60%), Gaps = 5/182 (2%) Query: 398 LARER-EALITQLQTSSNTDFLTGVLNRRAFFTHGQQQMTVAKRYGRELAVVLFDIDHFK 456 LA +R E L +L + TD LTG+ NR ++ A RYG+ L+++L DIDHFK Sbjct: 147 LAEQRVERLNEKLALLAVTDKLTGLANRMKLDEVLDNEINRADRYGKNLSLILLDIDHFK 206 Query: 457 QINDSYGHQAGDAILRGVAQSVSQLLRKVDVLARYGGEEFIILLPESSLLQAAAASEKLR 516 ++ND++GH GDA+L +A+ + +R D+ R+GGEEF+++LPE + AA +EKLR Sbjct: 207 EVNDNFGHSTGDAVLVRIAEILRANVRSSDLAGRWGGEEFLLILPECDMEDAARMAEKLR 266 Query: 517 AALAAGEFEIDGGRRIRVTASFGVAAENGGPGLEHLIKQADIALYRAKKLGRNRVKLAGE 576 + F + GRR T SFGVAA G L+ AD+ALYRAK GRNRV++A Sbjct: 267 LLIENYNF-VPVGRR---TCSFGVAAYQAGHDRTTLLTLADLALYRAKNTGRNRVEIAAN 322 Query: 577 EE 578 E Sbjct: 323 LE 324 >gi|300312078|ref|YP_003776170.1| hypothetical protein Hsero_2767 [Herbaspirillum seropedicae SmR1] Length = 534 Score = 122 bits (306), Expect = 8e-29, Method: Compositional matrix adjust. Identities = 65/159 (40%), Positives = 98/159 (61%), Gaps = 1/159 (0%) Query: 416 DFLTGVLNRRAFFTHGQQQMTVAKRYGRELAVVLFDIDHFKQINDSYGHQAGDAILRGVA 475 D LTG LNRR +++T A R L V L D+D FK+IND++GH GD +L + Sbjct: 367 DQLTGSLNRRGMDESLDREITNALRRDTPLCVALLDLDDFKRINDTHGHATGDEVLVHMV 426 Query: 476 QSVSQLLRKVDVLARYGGEEFIILLPESSLLQAAAASEKLRAALAAGEFEIDGGRRIRVT 535 Q + + LRK+DV+AR+GGEEF++L+PE++ A +++ L F + +R+ +T Sbjct: 427 QVIRETLRKLDVIARFGGEEFLVLMPETAPQDAVQIITRVQRELTKRIF-MHESQRLLIT 485 Query: 536 ASFGVAAENGGPGLEHLIKQADIALYRAKKLGRNRVKLA 574 S GVA + G L+K+AD+ALY+AK G+NR+ A Sbjct: 486 FSAGVAVYHPGESQADLLKRADVALYKAKNAGKNRIVFA 524 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,501,687 Number of extensions: 56923 Number of successful extensions: 321 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 268 Number of HSP's successfully gapped: 39 Length of query: 585 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 495 Effective length of database: 1,193,720 Effective search space: 590891400 Effective search space used: 590891400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)