BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0091.p (506 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde deh... 1046 0.0 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 303 3e-83 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 294 9e-81 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 286 4e-78 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 270 2e-73 >gi|300311221|ref|YP_003775313.1| NAD+ dependent acetaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 506 Score = 1046 bits (2705), Expect = 0.0, Method: Compositional matrix adjust. Identities = 505/506 (99%), Positives = 506/506 (100%) Query: 1 MNLADISKLGVRDPFKQRYDNFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVEL 60 MNLADISKLGVRDPFKQRYDNFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVEL Sbjct: 1 MNLADISKLGVRDPFKQRYDNFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVEL 60 Query: 61 ALDAAHAAKKSWGKTSPSERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPL 120 ALDAAHAAKKSWGKTSP+ERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPL Sbjct: 61 ALDAAHAAKKSWGKTSPTERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPL 120 Query: 121 AIDHFRYFAAAVRTQEGSICPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALA 180 AIDHFRYFAAAVRTQEGSICPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALA Sbjct: 121 AIDHFRYFAAAVRTQEGSICPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALA 180 Query: 181 AGNCVVLKPAEQTPASIMVLIELIADLIPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTG 240 AGNCVVLKPAEQTPASIMVLIELIADLIPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTG Sbjct: 181 AGNCVVLKPAEQTPASIMVLIELIADLIPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTG 240 Query: 241 ETTTGRLIMQYASQNLIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFALNQGEV 300 ETTTGRLIMQYASQNLIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFALNQGEV Sbjct: 241 ETTTGRLIMQYASQNLIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFALNQGEV 300 Query: 301 CTCPSRVLVQESIYERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGK 360 CTCPSRVLVQESIYERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGK Sbjct: 301 CTCPSRVLVQESIYERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGK 360 Query: 361 QEGAKVLAGGGREELGGDLASGYYVKPTVFQGNNKMRIFQEEIFGPVVSVTTFKDEEEAL 420 QEGAKVLAGGGREELGGDLASGYYVKPTVFQGNNKMRIFQEEIFGPVVSVTTFKDEEEAL Sbjct: 361 QEGAKVLAGGGREELGGDLASGYYVKPTVFQGNNKMRIFQEEIFGPVVSVTTFKDEEEAL 420 Query: 421 AIANDTLYGLGAGLWTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGREN 480 AIANDTLYGLGAGLWTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGREN Sbjct: 421 AIANDTLYGLGAGLWTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGREN 480 Query: 481 HKMMLDHYQQTKNLLVSYSPKALGFF 506 HKMMLDHYQQTKNLLVSYSPKALGFF Sbjct: 481 HKMMLDHYQQTKNLLVSYSPKALGFF 506 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 303 bits (775), Expect = 3e-83, Method: Compositional matrix adjust. Identities = 188/486 (38%), Positives = 266/486 (54%), Gaps = 17/486 (3%) Query: 18 RYDNF-IGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKSWGKTS 76 R D+F I GK+ PP E ++P CE+A +A DV+ A+ AA A +W TS Sbjct: 14 RLDHFYIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATS 73 Query: 77 PSERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQE 136 P ERA +L ++ + EL A A TL+ G PI A +PLA +H R R Sbjct: 74 PLERAALLGRVHALLLERSELFAQALTLEMGAPISYARTAHVPLAAEHLRV----ARDNL 129 Query: 137 GSICPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPAS 196 I EP+GV I PWN+PI K+ PALAAG VVLKP+E +P S Sbjct: 130 ADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLS 189 Query: 197 IMVLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQN 255 ++ E++ + +P GV N+V G G E G L+++ ++ I+ TG T G L+ Q A+ Sbjct: 190 ALLFAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVT 249 Query: 256 LIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFALNQGEVCTCPSRVLVQESIYE 315 + V ELGGKSPN+ D D G A N G+ C+ P+R++V + Sbjct: 250 VKRVAQELGGKSPNLVLPDA-----DLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALA 304 Query: 316 RFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGG-GREE 374 R E AL +A +K G+P D +T G A++ Q ++ I+ G Q+GA++LAGG GR + Sbjct: 305 RVHELALATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPD 364 Query: 375 LGGDLASGYYVKPTVF-QGNNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAG 433 LA GYY +PT+F + + M I QEEIFGPV+++ + EEA+AIANDT+YGLGA Sbjct: 365 ---GLAQGYYARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAH 421 Query: 434 LWTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQTKN 493 + RD + + IQ+G+V N Y + A FGGYKQSG GRE ++ Y + K Sbjct: 422 VQGRDKDQVRAVAARIQSGQVHLN-YPAWDPQAPFGGYKQSGNGREYGVEGMEEYMEVKA 480 Query: 494 LLVSYS 499 +L Y+ Sbjct: 481 ILGYYA 486 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 294 bits (753), Expect = 9e-81, Method: Compositional matrix adjust. Identities = 186/486 (38%), Positives = 266/486 (54%), Gaps = 22/486 (4%) Query: 21 NFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKK--SWGKTSPS 78 +FI G+ G+ ++P+ + A+ +A DV A+ AA A + +W K S Sbjct: 7 HFIAGEATEASDGQRMNLVNPINEEIYASAAQGTAADVARAVAAAKAQLEGGAWSKLSGL 66 Query: 79 ERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGS 138 +R +L K+A +E + +LLA + G+ E D+P AI H R A EG Sbjct: 67 QRGQLLHKLAALVERDTDLLADMDARAIGRSPMEPRMMDVPNAISHLRAAAGWANQMEGR 126 Query: 139 ICP----IDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTP 194 P + T +Y EP+GVVG I+PWN P+++ WK+A LAAG VV+KPAE+TP Sbjct: 127 TIPTAGYMGKPTLSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEETP 186 Query: 195 ASIMVLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYAS 253 S + L L + P GV+N+V G+G G+ L + +AKI+FTG GR I + A Sbjct: 187 QSALHLARLAQEAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAG 246 Query: 254 QNLIPVTLELGGKSPNIFFADVLDKDDDFFDKALEGFAM-FALNQGEVCTCPSRVLVQES 312 + VTLELGGKSP I F DD F+ AL G + +NQG+VC SR+LVQ S Sbjct: 247 EQFKRVTLELGGKSPQIVF------DDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRS 300 Query: 313 IYERFIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGG-G 371 + +RF + + A I G+P +G A + Q E++ YI G EGA +LAGG Sbjct: 301 LAQRFAKALAEAAAGITVGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGVS 360 Query: 372 REELGGDLASGYYVKPTVF-QGNNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGL 430 R + G++V+PT+F NN M I +EEIFGPV ++ +F EEEA+A+AND+ YGL Sbjct: 361 RPD------KGWFVQPTIFADANNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGL 414 Query: 431 GAGLWTRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQSGIGRENHKMMLDHYQQ 490 A +WT + RA R+ ++ G V NC+ A+ +GG K SG+GRE Y + Sbjct: 415 AATVWTTNLARAHRVAAGVKVGAVGVNCWSPLDANLPWGGIKDSGMGREGGLAGALAYTE 474 Query: 491 TKNLLV 496 K + V Sbjct: 475 EKTVTV 480 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 286 bits (731), Expect = 4e-78, Method: Compositional matrix adjust. Identities = 180/459 (39%), Positives = 259/459 (56%), Gaps = 27/459 (5%) Query: 19 YDNFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKSWGKTSPS 78 + +FI G+F P K + ++I P G+ +++ SA DV+ A+ AA AA W Sbjct: 33 FGHFIAGRFTQP-KAD-LDDIDPASGKLLAHLSQGSAADVDAAVQAAQAALPGWQALGGH 90 Query: 79 ERANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGS 138 RA L +A ++ + LLA ETLDNGKP+RE+ D+PL HF ++A + QE Sbjct: 91 GRARHLYALARTVQRHARLLAVLETLDNGKPLRESRDVDVPLVARHFYHYAGWAQLQESE 150 Query: 139 ICPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIM 198 + H P+GVVGQI+PWNFP+LM WK+APALA GN VVLKPAE T + + Sbjct: 151 ----------FPDHVPVGVVGQIVPWNFPLLMLAWKIAPALALGNTVVLKPAENTSLTAL 200 Query: 199 VLIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLI 257 + EL +P GV+NIV G G G + ++ I KIAFTG T GRLI + + + Sbjct: 201 LFAELAQQAGLPAGVLNIVTGDGA-TGAAVVAHPGIQKIAFTGSTEVGRLIREQTAGSGK 259 Query: 258 PVTLELGGKSPNIFFADVLDKDDDFFDKALEGFA-MFALNQGEVCTCPSRVLVQESIYER 316 +TLELGGKSP I F +D D A+EG NQG+VC SR+LVQE I++ Sbjct: 260 SLTLELGGKSPFIVF------EDADIDAAIEGVVDAIWFNQGQVCCAGSRLLVQEGIHDL 313 Query: 317 FIERALKRVAAIKQGNPLDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGREELG 376 FI R R+ +K G PLDK + +GA S QLE++ S ++ G +EGA+ + + Sbjct: 314 FIARLKTRMQKLKVGAPLDKCSDMGALISPVQLERVRSLVEQGVREGAQC-----HQVVL 368 Query: 377 GDLASGYYVKPTVFQG-NNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGAGLW 435 G + PT+ G + + EEIFGPV+ +F+ +EA+ +AN++ YGL A +W Sbjct: 369 DTPPGGCFYPPTLLTGVHPAATVASEEIFGPVLVAMSFRTPDEAVQLANNSRYGLAASIW 428 Query: 436 TRDGTRAFRMGREIQAGRVWTNCYHLYPAHAAFGGYKQS 474 + A + +++AG VW N + + A FGG ++S Sbjct: 429 SETIGLALGVAPQLKAGVVWINSTNQFDAAVGFGGVRES 467 Score = 65.1 bits (157), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 65/221 (29%), Positives = 94/221 (42%), Gaps = 6/221 (2%) Query: 22 FIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAAKKSWGKTSPSERA 81 +IGGK V P GE G +V + +D+ AA W SP RA Sbjct: 521 YIGGKQVRP-DGEVSMACHSTQGERLEDVGLGNRKDIR-NAVAAAVKAGGWTSASPHRRA 578 Query: 82 NMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGSICP 141 L IA+ + A E A G E A ++ +I + A EG++ Sbjct: 579 QGLYYIAENLSARTEEFARRIASTTGVTADEARA-EVDASIKRLFTYGAWADKFEGAVHQ 637 Query: 142 IDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIMVLI 201 A E +GVVG + P P+L + +AP +A GN VV+ P+EQ P L Sbjct: 638 PPMRGVALAMPEAIGVVGVVCPEEKPLLALISLVAPLIAMGNRVVVVPSEQAPLVATDLY 697 Query: 202 ELI--ADLIPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTG 240 +++ +DL P GV+NIV G E LA + + + G Sbjct: 698 QVLDTSDL-PAGVINIVTGKSAELLPVLAEHDEVEALWVRG 737 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 270 bits (690), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 168/469 (35%), Positives = 261/469 (55%), Gaps = 23/469 (4%) Query: 21 NFIGGKFVPPVKGEYFENISPVIGRAFCEVARSSAEDVELALDAAHAA-KKSWGKTSPSE 79 +FI ++V P GE + P G+ F +AR +A D++ A+ AA A + +WG+ S +E Sbjct: 4 HFIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDIDQAVRAARLAYEGAWGRLSAAE 63 Query: 80 RANMLLKIADRMEANLELLATAETLDNGKPIRETMAADIPLAIDHFRYFAAAVRTQEGSI 139 R+ +L+K+A +++ + E LA E D GKP+++ A D + +F ++A A G Sbjct: 64 RSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARA-DAAAIVRYFEFYAGAADKLHGDT 122 Query: 140 CPIDNDTYAYHFHEPLGVVGQIIPWNFPILMAVWKLAPALAAGNCVVLKPAEQTPASIMV 199 P EP GV G I+PWN+P+ + + ALAAGN V+KPAE SI+ Sbjct: 123 IPYQAGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSILR 182 Query: 200 LIELIADL-IPPGVVNIVQGFGVEAGKPLASNKRIAKIAFTGETTTGRLIMQYASQNLIP 258 + EL A++ P G +NIV G+G EAG L + + I+FTG G+L+++ A+++ P Sbjct: 183 VAELAAEVGFPEGALNIVTGYGHEAGDLLVKHPDVNHISFTGSPNVGKLVVRAAAEHHTP 242 Query: 259 VTLELGGKSPNIFFADVLDKDDDFFDKALEGFAMFAL--NQGEVCTCPSRVLVQESIYER 316 VTLELGGKSP I F +D D +A+ + A+ N G+ C+ SR+LVQ YE+ Sbjct: 243 VTLELGGKSPQIVF-----EDADM--EAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQ 295 Query: 317 FIERALKRVAAIKQGNP---LDKSTMIGAQASQEQLEKILSYIDIGKQEGAKVLAGGGRE 373 +ER + ++ G+ LD +I + Q E++ ++ +++G + A G + Sbjct: 296 VLERLSGLFSQLQVGSSQMDLDCGPLI----RKTQQERVAGFLSDAQRDGIALAAQG--K 349 Query: 374 ELGGDLASGYYVKPTVFQG-NNKMRIFQEEIFGPVVSVTTFKDEEEALAIANDTLYGLGA 432 G G+Y P + + + R+ QEE+FGPV++ F+DE A+A+AN T YGL A Sbjct: 350 IAAGVPTGGFYQAPALLRDVPPRHRLAQEEVFGPVLAAMPFEDEAHAIALANGTDYGLVA 409 Query: 433 GLWTRDGTRAFRMGREIQAGRVWTNCYHLYPA-HAAFGGYKQSGIGREN 480 +WTRDG R R+ R I++G+V+ N Y FGG K SG GRE Sbjct: 410 SVWTRDGGRQMRLARAIRSGQVFINNYGAGGGVELPFGGVKASGHGREK 458 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,528,477 Number of extensions: 63799 Number of successful extensions: 266 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 22 Length of query: 506 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 417 Effective length of database: 1,198,455 Effective search space: 499755735 Effective search space used: 499755735 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)