BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0088.p (685 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthet... 1409 0.0 gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) syntheta... 125 8e-30 >gi|300311219|ref|YP_003775311.1| glutamine-dependent NAD synthetase [Herbaspirillum seropedicae SmR1] Length = 685 Score = 1409 bits (3646), Expect = 0.0, Method: Compositional matrix adjust. Identities = 682/685 (99%), Positives = 684/685 (99%) Query: 1 MQSPFFNLYSHGFARVAVAVPDCKVADPGFNAQRTIALAEQAAARGAVLVSFPELGLSAY 60 MQSPFFNLYSHGFARVAVAVPDCKVADPGFNAQRTIALAEQAAARGAVLVSFPELGLSAY Sbjct: 1 MQSPFFNLYSHGFARVAVAVPDCKVADPGFNAQRTIALAEQAAARGAVLVSFPELGLSAY 60 Query: 61 SCEDLFQQRALLDASLHALQSVLEASMRIPAALIVGLPLKVDHQLYNCAVVLHHGRILGV 120 SCEDLFQQRALLDASL ALQSVLEASMRIPAALIVGLPLKVDHQLYNCAVVLHHGRILGV Sbjct: 61 SCEDLFQQRALLDASLQALQSVLEASMRIPAALIVGLPLKVDHQLYNCAVVLHHGRILGV 120 Query: 121 VPKSYLPNYSEFYEARQFSSADCAVTRSVHLLGQEVGFGSHLLFEIRNIPDFRFHIEICE 180 VPKSYLPNYSEFYEARQFSSADCAVTRSVHLLGQEVGFGSHLLFEIRNIPDFRFHIEICE Sbjct: 121 VPKSYLPNYSEFYEARQFSSADCAVTRSVHLLGQEVGFGSHLLFEIRNIPDFRFHIEICE 180 Query: 181 DVWVPIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGE 240 DVWVPIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGE Sbjct: 181 DVWVPIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGE 240 Query: 241 STTDLAWDGQALICENGELLAESERFAQGGHVIYADVDLERLSRERFHQTTFGQSVRRHA 300 STTDLAWDGQALICENGELLAESERFAQGGHVIYADVDLERLSRERFHQTTFGQSVRRHA Sbjct: 241 STTDLAWDGQALICENGELLAESERFAQGGHVIYADVDLERLSRERFHQTTFGQSVRRHA 300 Query: 301 DEVRRFEVVGFEVQIPQQQALPLQRRVARFPYVPANAEQRELRCREVYSIQVQALVQRLS 360 DEVRRFEVVGFEVQIPQQQALPLQRRVARFPYVPANAEQRELRCREVYSIQVQALVQRLS Sbjct: 301 DEVRRFEVVGFEVQIPQQQALPLQRRVARFPYVPANAEQRELRCREVYSIQVQALVQRLS 360 Query: 361 SAGLKKVVIGVSGGLDSTHALLVCAKAMDKLGLPRSNILAYTMPGFATSSRTLVQAHQLM 420 SAGLKKVVIGVSGGLDSTHALLVCAKAMDKLGLPRSNILAYTMPGFATSSRTLVQAHQLM Sbjct: 361 SAGLKKVVIGVSGGLDSTHALLVCAKAMDKLGLPRSNILAYTMPGFATSSRTLVQAHQLM 420 Query: 421 AQVGCSAQEIDIRPSCEQMLKDLGHPYSRGEPVYDITFENVQAGERTNHLFRLANHHGAI 480 AQVGCSAQEIDIRPSCEQMLKDLGHPYSRGEPVYDITFENVQAGERTNHLFRLANHHGAI Sbjct: 421 AQVGCSAQEIDIRPSCEQMLKDLGHPYSRGEPVYDITFENVQAGERTNHLFRLANHHGAI 480 Query: 481 VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITHLVRWVAESGSLELKQPEVLIH 540 VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITH+VRWVAESGSLELKQPEVLIH Sbjct: 481 VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITHMVRWVAESGSLELKQPEVLIH 540 Query: 541 VLETEISPELVPGASDGEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVAFLAHSAWHD 600 VLETEISPELVPGASDGEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVAFLAHSAWHD Sbjct: 541 VLETEISPELVPGASDGEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVAFLAHSAWHD 600 Query: 601 RGVGHWPDGLHGAHNEYSLPQIKKNLGIFIYRFFKTSQFKRSCVPNAPKVGSGGSLSPRG 660 RGVGHWPDGLHGAHNEYSLPQIKKNLGIF+YRFFKTSQFKRSCVPNAPKVGSGGSLSPRG Sbjct: 601 RGVGHWPDGLHGAHNEYSLPQIKKNLGIFVYRFFKTSQFKRSCVPNAPKVGSGGSLSPRG 660 Query: 661 DWRAPSDGEATVWLQDLERIPDLEG 685 DWRAPSDGEATVWLQDLERIPDLEG Sbjct: 661 DWRAPSDGEATVWLQDLERIPDLEG 685 >gi|300313858|ref|YP_003777950.1| NH(3)-dependent NAD(+) synthetase [Herbaspirillum seropedicae SmR1] Length = 565 Score = 125 bits (315), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 149/590 (25%), Positives = 236/590 (40%), Gaps = 100/590 (16%) Query: 15 RVAVAVPDCKVADPGFNAQRTIALAEQAAARGAVLVSFPELGLSAYSCEDLFQQRALLDA 74 R+A+A + + D N + ++AA GA LV FPEL + Y DL ++ LD Sbjct: 3 RIAIAQCNPTIGDFEGNLALHLDGLQRAAQAGADLVVFPELSICGYYPGDLLEEARFLDR 62 Query: 75 SLHALQSVLEASMRIPA-ALIVGLPLK---VDHQLYNCAVVLHHGRILGVVPKSYLPNYS 130 + LQ V++A+ R+P ++G P + L+N + + GRI+ K LP Y Sbjct: 63 AEATLQDVIDATRRLPGLTAVIGAPRRNPGPGKPLFNALLAVREGRIVAEYHKQLLPVYG 122 Query: 131 EFYEARQFSSADCAVTRSVHLLGQEVGFGSHLLFEIRNIPDFRFHIEICEDVW------V 184 F + R F A + L G VGF ICED W Sbjct: 123 VFDDGRHF-EAGPETACVLPLKGMRVGF------------------LICEDGWNDDLRSY 163 Query: 185 PIPPSSFAALAGATVLVNLSASNIVVGKSGYRHQLVSQQSARCLAAYLYSSAGNGESTTD 244 + P A A A ++V+++AS VGK RH L R A LY + G+ Sbjct: 164 RVNPFEKLAQADAQLVVSINASPSDVGKRSQRHALFQAACLRRQMALLYVNQVGGQD--Q 221 Query: 245 LAWDGQALICENGELLA-ESERFAQGGHVIYADVDLERLSRERFHQTTFGQSVRRHADEV 303 L +DG + + LA E+ R A E F F Sbjct: 222 LVYDGGSFAMSPLQGLAFEAARLA-----------------EDFQVLAF------EGGRF 258 Query: 304 RRFEVVGFEVQIPQQQALPLQRRVARFPYVPANAEQRELRCREVYSIQVQALVQRLSSAG 363 R + VG I Q A P E R++ + L G Sbjct: 259 LRSDGVGAPQCIDQTMATP------------------EFHRRQI----LLGLRDYARRCG 296 Query: 364 LKKVVIGVSGGLDSTHALLVCAKAMDKLGLPRSNILAYTMPGFATSSRTLVQAHQLMAQV 423 KVV+G SGG+DS + + A+A+ N++A TMP +S+ ++ + L + Sbjct: 297 FGKVVVGSSGGIDSAVTIALAAEALGA-----QNVVAVTMPSVFSSAGSVSDSETLCQAL 351 Query: 424 GCSAQEIDIRPSCEQMLKDL--GHPYSRGEPVYDITFENVQAGERTNHLFRLANHHGAIV 481 G + R ++++ G + G P+ + EN+QA R L +N + A++ Sbjct: 352 GVTL----FRHPIADLVREFEDGFATAFGAPLRGLPLENLQARVRGAILMEYSNGYDALL 407 Query: 482 IGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLITHLVRWVAESGSLELKQPEVLIHV 541 + TG+ SE+++G+CT GD + + KT + L R + E+ ++ Sbjct: 408 LTTGNKSEISVGYCTL-YGDTNGGLGLIGDLYKTEVYALARHLNAQAGREIIPAAIIDKA 466 Query: 542 LETEISPELVPGASDGEPGQRTQDFIGPYELQDFNLYYILRYGFTPAKVA 591 E++P+ QR D + PYE+ D L + + PA A Sbjct: 467 PSAELAPD-----------QRDTDSLPPYEVLDEILKWHIEGSRLPADEA 505 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,212,375 Number of extensions: 94465 Number of successful extensions: 262 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 258 Number of HSP's successfully gapped: 3 Length of query: 685 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 593 Effective length of database: 1,184,250 Effective search space: 702260250 Effective search space used: 702260250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)