BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0051.p (548 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 1052 0.0 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 139 5e-34 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 133 3e-32 gi|300312566|ref|YP_003776658.1| multidrug resistance protein pr... 114 3e-26 gi|300310856|ref|YP_003774948.1| major facilitator superfamily p... 114 3e-26 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 1052 bits (2720), Expect = 0.0, Method: Compositional matrix adjust. Identities = 531/532 (99%), Positives = 531/532 (99%) Query: 17 MALHTDSAHSSGQVLPFRESFLATLGICFVIMLVALDQTIVGTALPTIVAELKGFELYAW 76 MALHTDSAHSSGQVLPFRES LATLGICFVIMLVALDQTIVGTALPTIVAELKGFELYAW Sbjct: 1 MALHTDSAHSSGQVLPFRESLLATLGICFVIMLVALDQTIVGTALPTIVAELKGFELYAW 60 Query: 77 VATAYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQG 136 VATAYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQG Sbjct: 61 VATAYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQG 120 Query: 137 IGGGMLVGTCFASVADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFY 196 IGGGMLVGTCFASVADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFY Sbjct: 121 IGGGMLVGTCFASVADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFY 180 Query: 197 CNLPIGVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTS 256 CNLPIGVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTS Sbjct: 181 CNLPIGVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTS 240 Query: 257 GMLVLLAVSVAAFYALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAP 316 GMLVLLAVSVAAFYALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAP Sbjct: 241 GMLVLLAVSVAAFYALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAP 300 Query: 317 LLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGI 376 LLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGI Sbjct: 301 LLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGI 360 Query: 377 VLCDRVTGKGFLLAVMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGML 436 VLCDRVTGKGFLLAVMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGML Sbjct: 361 VLCDRVTGKGFLLAVMLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGML 420 Query: 437 GTAITGTLVSQMYGAGVEKALEGDHAAQWLKDFSDPEVLVNHDIQSVLLAQLMQAGHDGS 496 GTAITGTLVSQMYGAGVEKALEGDHAAQWLKDFSDPEVLVNHDIQSVLLAQLMQAGHDGS Sbjct: 421 GTAITGTLVSQMYGAGVEKALEGDHAAQWLKDFSDPEVLVNHDIQSVLLAQLMQAGHDGS 480 Query: 497 ALLEAAREALVGAIHMGIAIAIVAALVGLWMVRRVPPIRFDQDKKVEPVMSE 548 ALLEAAREALVGAIHMGIAIAIVAALVGLWMVRRVPPIRFDQDKKVEPVMSE Sbjct: 481 ALLEAAREALVGAIHMGIAIAIVAALVGLWMVRRVPPIRFDQDKKVEPVMSE 532 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 139 bits (351), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 128/439 (29%), Positives = 202/439 (46%), Gaps = 19/439 (4%) Query: 38 LATLGICFVIMLVALDQTIVGTALPTIVAELKG-FELYAWVATAYLLTSVITVPIFGRLG 96 L L I FV+ + LD T+V L I L W+ Y LT + + G L Sbjct: 32 LVALAIGFVMAM--LDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVGGALA 89 Query: 97 DYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASVADLFPD 156 D FG + + + +FV AS LCG A + FL++AR LQG+G + + + + ++ D Sbjct: 90 DRFGARRLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTHVYED 149 Query: 157 PRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFYCNLPIGVISLFFVWRFVPHI 216 RVR R + S + G+A AVGP +GG L LGWR VF N+P+G++ L + +P + Sbjct: 150 DRVRARMLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKLIPQV 209 Query: 217 RHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPKRGVTSG-MLVLLAVSVAAFYALWKW 275 A + G ++ L L ++ P G TS +L LAV+++A AL + Sbjct: 210 GGHARALNLSSHLAGVVM----LAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAALIRH 265 Query: 276 EQRAAQPILPFEMLRDRALASLFLLSVLAGFAMFSMLMYAPLLFQGGFGLSPREAGLLIT 335 E R P+LP + A+ + L F ++ L Y L Q G G + GL + Sbjct: 266 ELRGIAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLV 325 Query: 336 PLVACITVASIVNGRIITRI-PNPNLMMNLGFALI-AASCLGIVLCDRVTGKGFLLAVML 393 P++A I ++++GR+ P L++ L + A +G T L V Sbjct: 326 PMMAVIFFGNLMSGRMSAHWGPRVPLLLGLSVGTVFTALAMGF---SPATPYWLLALVCA 382 Query: 394 AGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGMLGTAITGTLVSQMYGAGV 453 + G+ +P +T Q A + H A A L + R IG ++G A+ G ++ + G + Sbjct: 383 SANLGISTAIPAMTTVVMQVAGKAHANSAAAALNANRQIGALVGVALMGAILHSVTGWNL 442 Query: 454 EKAL------EGDHAAQWL 466 L G +A WL Sbjct: 443 RNPLAFGVMAAGYASALWL 461 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 133 bits (335), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 127/448 (28%), Positives = 201/448 (44%), Gaps = 17/448 (3%) Query: 18 ALHTDSAHSSGQVLPFRESFLATLGICFVIMLVALDQTIVGTALPTIVAELK-GFELYAW 76 A+ S H + + LP ++ A L + + D TI+ ALP I L+ + W Sbjct: 9 AMELFSDHPNDEGLPGKQRRRAMLAVMTATTMAVFDGTIINVALPQISQALQTSAAVSIW 68 Query: 77 VATAYLLTSVITVPIFGRLGDYFGRKPFVIASIIVFVGASALCGMANSMLFLVLARGLQG 136 V+ AYLL + +T+ F L G + A + VF AS C ++ S+ L+L R LQG Sbjct: 69 VSNAYLLATAMTLAAFAALSSRIGFRAQFSAGLAVFTLASLGCALSGSVTLLILMRILQG 128 Query: 137 IGGGMLVGTCFASVADLFPDPRVRLRWQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFY 196 +GG ++ A +FP+ R+ R + +V +TAVGP LGG L LGW W+F Sbjct: 129 LGGAAMLSIAPAIHRTIFPN-RLLGRILGLNAVLVAASTAVGPVLGGTLLAMLGWEWIFA 187 Query: 197 CNLPIGVISLFFVWRFVPHIRHMQHAGKMRLDWPGALLISLSLGSLQLLVELLPK----- 251 N+P+GV++++ W VP R A D GAL +L++G+L + + + Sbjct: 188 INVPLGVLAVWLAWNAVPDTRKPAQAA---FDLAGALWSALAMGALIMAADTCARFAEPG 244 Query: 252 --RGVTSGMLVLLAVSVAAFYALWKWEQRAAQPILPFEMLRDRALASLFLLSVLAGFAMF 309 G ++ A A + ++RAA P+LP ++ + SL L+ LA F Sbjct: 245 QGSGAAFTAAAYAVTALLAGLAFVRGQRRAADPLLPLDIFANPRF-SLAALTSLASFVGQ 303 Query: 310 SMLMYA-PLLFQGGFGLSPREAGLLITPLVACITVASIVNGRIITRIPNPNLMMNLGFAL 368 + A P LFQ +G + E+ L TP I + + GR+ R L Sbjct: 304 GIAFVALPFLFQNAYGYTAFESAALFTPWPLGIVLVAPHAGRLADRHSPALLSSLGLALF 363 Query: 369 IAASCLGIVLCDRVTGKGFLLAVMLAG-GFGLGLVLPNLTVFAQQAAAREHLGIATALLQ 427 L +L D+ ++ G GFG N + +RE G A+ +L Sbjct: 364 ALGLVLLALLPDQAQPWDIGWRALVCGMGFGFFQSPNNREMLGN--VSRERSGNASGVLA 421 Query: 428 SLRMIGGMLGTAITGTLVSQMYGAGVEK 455 +R G LG A+ G +++ A V + Sbjct: 422 IMRTFGQCLGAALLGVILAVRTAAAVAQ 449 >gi|300312566|ref|YP_003776658.1| multidrug resistance protein protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 114 bits (284), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 117/413 (28%), Positives = 181/413 (43%), Gaps = 43/413 (10%) Query: 52 LDQTIVGTALPTIVAELKGFELY-AWVATAYLLTSVITVPIFGRLGDYFGRKPFVIASII 110 LD TI ++ TI L +V T+Y + ITVP+ G L + FG I S++ Sbjct: 47 LDTTIANVSVSTIAGALGASTSQGTYVITSYAVAEAITVPLTGWLANRFGTVRVFILSMV 106 Query: 111 VFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASVADLFPDPRVRLR---WQIIL 167 +F SALCGMANS+ L+ R LQG+ GG L+ + +FP + W + Sbjct: 107 MFGLFSALCGMANSLGMLIAFRVLQGLAGGPLMPLSQTLLLRIFPKEKAPAAVGLWAMTT 166 Query: 168 SVSFGIATAVGPSLGGFLTQGLGWRWVFYCNLPIGVISLFFVWRFVPHIRHMQHAGKMRL 227 V A GP LGG L W ++F+ N+P+ +I + WR + R+ K+ + Sbjct: 167 LV----APIAGPILGGVLCDQYSWPYIFWINVPVALICGYIGWRMLK--RYETKMSKLPI 220 Query: 228 DWPGALLISLSLGSLQLLV-ELLPKRGVTSGMLVLLAVSVAAFY-ALWKWEQRAAQPILP 285 D G +L+ + + +LQL++ E K S +VLLAV+ F+ A WE PI+ Sbjct: 221 DRVGLVLMVVWVAALQLMLDEGKEKDWFASTEIVLLAVTAVVFFIAFLIWELTERNPIVD 280 Query: 286 FEMLRDRALASLFLLSVLAGFAMFSMLMYAPLLFQGGFGLSPREAGLL--ITPLVACITV 343 + R R + LA A F ++ PL QG G + +G + +T ++A +T Sbjct: 281 LRVFRHRGFTASVTTICLAFGAFFGSVVLTPLWLQGYMGYTATWSGCISALTGILAVMTA 340 Query: 344 ASIVNGRIITRIPNPNLMMNLGFALIAASCLGIVLCDRVTGKGFLLAV------------ 391 + ++ T I L+ C+G+ +T FL A Sbjct: 341 PMV--AKMSTTIDARKLV-----------CIGVTWLGFIT---FLRAFNTTDMSFFQIGW 384 Query: 392 -MLAGGFGLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGMLGTAITGT 443 + G GL L LT A + E A L+ R + G + T+I T Sbjct: 385 PLFLQGIGLPLFFVPLTGLALSSVNEEETASAAGLMSFCRTMAGAIATSIVNT 437 >gi|300310856|ref|YP_003774948.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 446 Score = 114 bits (284), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 119/450 (26%), Positives = 204/450 (45%), Gaps = 19/450 (4%) Query: 52 LDQTIVGTALPTIVAELKGFELYAWVA-TAYLLTSVITVPIFGRLGDYFGRKPFVIASII 110 +D T++ T+LP + ++ + +A T+YL++ + +PI G + D FG ++I Sbjct: 1 MDSTVISTSLPALALDMNVDPISLKLALTSYLVSLAVFIPISGWMADRFGSLTIFRSAIC 60 Query: 111 VFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASVADLFPDPRVRL-RWQIILSV 169 VF+ S LCG+++++ V AR LQGIGG M+V + L +V L + L++ Sbjct: 61 VFMLGSILCGVSSTLPQFVAARFLQGIGGAMMVPV--GRLVILRSVEKVNLVKALSYLTI 118 Query: 170 SFGIATAVGPSLGGFLTQGLGWRWVFYCNLPIGVISLFFVWRFVPHIRHMQHAGKMRLDW 229 +GP LGGF+T WRW+F+ N+PI ++ L+ RF+P ++ Q A LD Sbjct: 119 PALFGPVLGPPLGGFITTYFHWRWIFFINIPISILGLYLATRFIPDLKAEQVA---PLDL 175 Query: 230 PGALLISLSLGSLQLLVELLPKRGVTSGMLVLLAVSVA-AFYALWKWE-QRAAQPILPFE 287 G LL +L SL ++ R + SG + L V F A + W +RA+ P++ Sbjct: 176 KGFLLSALGC-SLLMMGLATAGRHLVSGEVALTCVLAGLVFLAAYVWHARRASAPLIDLA 234 Query: 288 MLRDRALASLFLLSVLAGFAMFSMLMYAPLLFQGGFGLSPREAGLLITPLVACITVASIV 347 +L+ L + + ++ PL+ Q GFG +P +G+L A + Sbjct: 235 LLKIPTLRAGVFGGSFYRVGIGAIPFLLPLMLQLGFGYTPFHSGMLTCASAAGAMFMKTI 294 Query: 348 NGRIITRIPNPNLMMNLGFALIAASCLGIVLCDRVTGKGFLLAVMLAGGFGLGLVLPNLT 407 R + R +++ G AL I T ++ ++L GG L +L Sbjct: 295 AARFLRRYGFRRVLLVNG-ALGVLGLWAIATFTPQTPYLLMVVILLIGGCFRSLQFTSLN 353 Query: 408 VFAQQAAAREHLGIATALLQSLRMIGGMLGTAITGTLVSQMYGAGVEKALEGDHAAQWLK 467 + + L AT++ + + +G + G + + ++G HA + Sbjct: 354 AISYADVDKLRLSQATSMASVAQQLSIGMGVVLAG------FALQMSNEIQG-HATLVAQ 406 Query: 468 DFSDPEVLVNHDIQSVLLAQLMQAGHDGSA 497 DF P LV + + L L Q HD A Sbjct: 407 DFW-PAFLVVGAVAACALLMLRQLPHDAGA 435 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.142 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,569,645 Number of extensions: 61847 Number of successful extensions: 447 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 416 Number of HSP's successfully gapped: 17 Length of query: 548 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 458 Effective length of database: 1,193,720 Effective search space: 546723760 Effective search space used: 546723760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)