BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0044.p (340 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedi... 691 0.0 gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum... 129 3e-31 gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily pro... 102 5e-23 >gi|300311178|ref|YP_003775270.1| lipase [Herbaspirillum seropedicae SmR1] Length = 340 Score = 691 bits (1783), Expect = 0.0, Method: Compositional matrix adjust. Identities = 340/340 (100%), Positives = 340/340 (100%) Query: 1 MTSFKQLAAVAAIGLVFGNAYAAGDAGVEPTTQAFLQALEAGGGKPLEQLSPADARAVLV 60 MTSFKQLAAVAAIGLVFGNAYAAGDAGVEPTTQAFLQALEAGGGKPLEQLSPADARAVLV Sbjct: 1 MTSFKQLAAVAAIGLVFGNAYAAGDAGVEPTTQAFLQALEAGGGKPLEQLSPADARAVLV 60 Query: 61 GAQASVKLQLPKADVSEKTISADGQQIKLTIVRPAGSAKKTLPAFMFFHGGGWVLGDFPT 120 GAQASVKLQLPKADVSEKTISADGQQIKLTIVRPAGSAKKTLPAFMFFHGGGWVLGDFPT Sbjct: 61 GAQASVKLQLPKADVSEKTISADGQQIKLTIVRPAGSAKKTLPAFMFFHGGGWVLGDFPT 120 Query: 121 HERLVRDLVANSGAVAVFVNYTPSPEAHYGVAINQAYAATKWVAEHGKEINVDGKRLAVA 180 HERLVRDLVANSGAVAVFVNYTPSPEAHYGVAINQAYAATKWVAEHGKEINVDGKRLAVA Sbjct: 121 HERLVRDLVANSGAVAVFVNYTPSPEAHYGVAINQAYAATKWVAEHGKEINVDGKRLAVA 180 Query: 181 GNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFETASYNQFANGYFLTKNMMKWF 240 GNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFETASYNQFANGYFLTKNMMKWF Sbjct: 181 GNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFETASYNQFANGYFLTKNMMKWF 240 Query: 241 WDSYTTDAAKRKEITASPLNATPAQLSGLPPTLIQTAEKDVLRDEGEEYGRKLAAAGVAV 300 WDSYTTDAAKRKEITASPLNATPAQLSGLPPTLIQTAEKDVLRDEGEEYGRKLAAAGVAV Sbjct: 241 WDSYTTDAAKRKEITASPLNATPAQLSGLPPTLIQTAEKDVLRDEGEEYGRKLAAAGVAV 300 Query: 301 TSVRYNGMIHDFGLLNVLAKVPAVQTAMRQAGEELKFRLK 340 TSVRYNGMIHDFGLLNVLAKVPAVQTAMRQAGEELKFRLK Sbjct: 301 TSVRYNGMIHDFGLLNVLAKVPAVQTAMRQAGEELKFRLK 340 >gi|300311659|ref|YP_003775751.1| esterase/lipase [Herbaspirillum seropedicae SmR1] Length = 317 Score = 129 bits (325), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 94/295 (31%), Positives = 142/295 (48%), Gaps = 16/295 (5%) Query: 28 VEPTTQAFLQALEAG----GGKPLEQLSPADARAVLVGAQASVKLQLPKADVSEKTI--- 80 + P AFL +EAG +P+ + S ARA A+ L LP D++E+ Sbjct: 3 LHPDLDAFLALVEAGIQSGRQQPMHRKSVHQARADY--EAAAPMLDLPPDDLAERRALEI 60 Query: 81 -SADGQQIKLTIVRPAGSAKKTLPA-FMFFHGGGWVLGDFPTHERLVRDLVANSGAVAVF 138 + DG +I + PA ++FHGGG+ +G +H+ L L A + + Sbjct: 61 PTRDGARIGARLYAPAPLDAAAPQPVLLYFHGGGYCVGSLDSHDTLCSALAARTPCCVLA 120 Query: 139 VNYTPSPEAHYGVAINQAYAATKWVAEHGKEINVDGKRLAVAGNSVGGN-MAAVVALMAK 197 V+Y +PE + A A A W+ +HG + + RLAV G+S GG AA+ + Sbjct: 121 VDYRLAPEHRFPTAFEDAADAYDWLLQHGPALGLATNRLAVGGDSAGGTLAAALALRARQ 180 Query: 198 DKGGPALRSEVLLWPVTDANFETASYNQFANGYFLTKNMMKWFWDSYTTDAAKRKEITAS 257 P L +VLL+P T A+ + S+ +FA GY L ++W + +R++ + Sbjct: 181 AARAPCL--QVLLYPCTSAHQDGDSHRRFATGYLLEGETLQWMFGHTLNGDHERQDWRFA 238 Query: 258 PLNATPAQLSGLPPTLIQTAEKDVLRDEGEEYGRKLAAAGVAVTSVRYNGMIHDF 312 PL A A +SG P L+ + D L DEG Y KL AGV V + GM+HDF Sbjct: 239 PLQA--ADVSGAAPALVVIGDHDPLLDEGMAYAEKLRQAGVPVQLDIHPGMVHDF 291 >gi|300309659|ref|YP_003773751.1| esterase-lipase superfamily protein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 102 bits (254), Expect = 5e-23, Method: Compositional matrix adjust. Identities = 71/209 (33%), Positives = 103/209 (49%), Gaps = 5/209 (2%) Query: 103 PAFMFFHGGGWVLGDFPTHERLVRDLVANSGAVAVF-VNYTPSPEAHYGVAINQAYAATK 161 P M+FHGGG+V G + R +A G +AV V Y +P + A A+AA Sbjct: 84 PLVMYFHGGGFVSGSLEDADTPAR-FIAEHGQLAVLSVGYALAPAKPFPAAPEDAFAAAT 142 Query: 162 WVAEHGKEINVDGKRLAVAGNSVGGNMAAVVALMAKDKGGPALRSEVLLWPVTDANFETA 221 W ++ E+ VD R+ VAG+ GG +AA + LM +D+ G L ++VL+ P+ D + Sbjct: 143 WACKNATELKVDLDRMVVAGDDAGGCLAASLTLMTRDRCGKPLAAQVLISPMLDPSMRLL 202 Query: 222 SYNQFANGYFLTKNMMKWFWDSYTTDAAKRKEITASPLNATPAQLSGLPPTLIQTAEKDV 281 LT + Y +R A+PL + +L+GLPP I TAE+DV Sbjct: 203 GDAARLQSD-LTAETCAASYRQYLPQTMQRLHPYAAPLEVS--RLAGLPPAFIATAERDV 259 Query: 282 LRDEGEEYGRKLAAAGVAVTSVRYNGMIH 310 L E E+Y L AGV R+ G+ H Sbjct: 260 LCPEAEKYASSLIRAGVPTQVSRFAGITH 288 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,138,020 Number of extensions: 50630 Number of successful extensions: 211 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 207 Number of HSP's successfully gapped: 4 Length of query: 340 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 254 Effective length of database: 1,212,660 Effective search space: 308015640 Effective search space used: 308015640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)