BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0041.p (297 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311176|ref|YP_003775268.1| 2-dehydropantoate 2-reductase [... 593 e-171 gi|300310480|ref|YP_003774572.1| 2-dehydropantoate 2-reductase [... 122 3e-29 gi|300310685|ref|YP_003774777.1| 2-ketopantoate reductase [Herba... 86 4e-18 >gi|300311176|ref|YP_003775268.1| 2-dehydropantoate 2-reductase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 593 bits (1529), Expect = e-171, Method: Compositional matrix adjust. Identities = 294/297 (98%), Positives = 295/297 (99%) Query: 1 MKVAVMGAGAVGCYFAGMLARVGHQVALVGRPQHVEAFNTRGLRLETKAFDETIAVKAST 60 MKVAVMGAGAVGCYFAGMLARVGHQVALVGRPQHVEAFNTRGLRLETKAFDETIAVKAST Sbjct: 1 MKVAVMGAGAVGCYFAGMLARVGHQVALVGRPQHVEAFNTRGLRLETKAFDETIAVKAST 60 Query: 61 DAAIAADAELVLFCVKSPDTETAGAQLAPHLAPDTLVLSLQNGTDNAERLRTVMTQPVAA 120 DAAIAADAELVLFCVKSPDTETAGAQLAPHLAPDTLVLSLQNGTDNAERLRTVM QPVAA Sbjct: 61 DAAIAADAELVLFCVKSPDTETAGAQLAPHLAPDTLVLSLQNGTDNAERLRTVMAQPVAA 120 Query: 121 AVVYVATEMAGPGHVKHHGRGELVIEPAAGSRDAADALVAVGVPTEISDNVRGELWLKLI 180 AVVYVATEMAGPGHVKHHGRGELVIEPAAGSRDAADALVA GVPTEISDNVRGELWLKLI Sbjct: 121 AVVYVATEMAGPGHVKHHGRGELVIEPAAGSRDAADALVAAGVPTEISDNVRGELWLKLI 180 Query: 181 LNCAYNALSAIAQLPYGKLVEGPGIPEVIAQLVTECKAVAAAEGVTLADDVDSAVRKIVQ 240 LNCAYNALSAIAQLPYGKLVEGPGIPEVIAQLVTECKAVAAAEGVTLADDVDSAVRKIVQ Sbjct: 181 LNCAYNALSAIAQLPYGKLVEGPGIPEVIAQLVTECKAVAAAEGVTLADDVDSAVRKIVQ 240 Query: 241 TMPGQFSSTAQDLARGKPSEIDHLNGFIVARAQAHGLSAPANLTVHALVKLIESKAR 297 TMPGQFSSTAQDLARGKPSEIDHLNGFIVARAQAHGLSAPANLTVHALVKLIESKA+ Sbjct: 241 TMPGQFSSTAQDLARGKPSEIDHLNGFIVARAQAHGLSAPANLTVHALVKLIESKAQ 297 >gi|300310480|ref|YP_003774572.1| 2-dehydropantoate 2-reductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 122 bits (307), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 96/305 (31%), Positives = 147/305 (48%), Gaps = 16/305 (5%) Query: 1 MKVAVMGAGAVGCYFAGMLARVGHQVALVG-RPQHVEAFNTRGLRLETKAFDETIAVKAS 59 MK+A++GAGA+G FA LA G V+L+ P +EA ++ GL L + T+ V Sbjct: 1 MKIAIVGAGAMGGLFAARLALAGQDVSLIEVAPASIEAISSHGLSLTDEDGAHTVRVPVG 60 Query: 60 TDAAIAADAELVLFCVKSPDTETAGAQLAPHLAPDTLVLSLQNGTDNAERLRTVM-TQPV 118 + +L++ K T+ A A +A P T LS+QNG NA+ + V+ + + Sbjct: 61 RADSFQDAFDLIILFTKGFHTQAAIASVAHLAGPHTWALSVQNGLGNADLIAEVIPRERI 120 Query: 119 AAAVVYVATEMAGPGHVKHHGRGELVI-----EPAAGSRDAADALVAVGVPTEISDNVRG 173 + + ++ PG V HG G++ I +P+A A A G+P V+ Sbjct: 121 IVGMTNLPADLRAPGVVHSHGEGQVRIWTLSGQPSAQLTQVAADFNAAGLPCSADPLVQQ 180 Query: 174 ELWLKLILNCAYNALSAIAQLPYGKLVEGPGIPEVIAQLVTECKAVAAAEG-----VTLA 228 +W KL N A NA+ A+ L G E+ ++ E AVA A G V + Sbjct: 181 AIWEKLAFNAALNAICAVTGLTVGAAGASASGRELALRIAAETVAVAQAAGIDARLVQVE 240 Query: 229 DDVDSAVRKIVQTMPGQFSSTAQDLARGKPSEIDHLNGFIVARAQAHGLSAPANLTVHAL 288 D ++ A R + P S QD+ G+ +EID +NG +V A+ GL P T+H L Sbjct: 241 DAIEHAFRTHLHHKP----SMLQDIEAGRRTEIDFINGAVVREAERLGLPVPTTQTLHQL 296 Query: 289 VKLIE 293 VKL+E Sbjct: 297 VKLME 301 >gi|300310685|ref|YP_003774777.1| 2-ketopantoate reductase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 85.9 bits (211), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 77/321 (23%), Positives = 146/321 (45%), Gaps = 36/321 (11%) Query: 1 MKVAVMGAGAVGCYFAGMLARVGHQVALVGRPQHVEAFNTRGLRLETKAFDETIAVKAST 60 +++ + GAGA+G A LA GHQV+++ R Q ++A GL L T + + Sbjct: 15 LRICIAGAGAIGGTLAVRLAAAGHQVSVLARGQTLQAIREHGLTLHD--LHGTAHARPAA 72 Query: 61 DAAIAADAELVLF-CVKSPDTETAGAQLAPHLAPDTLVLSLQNGTD-----------NAE 108 D + + V+F C KS D Q+AP + T+V+ NG + + Sbjct: 73 DHRPSFGVQDVIFLCAKSQDMAGLLPQVAPMVGETTVVIPTNNGVPWWYFHREGGRFDGQ 132 Query: 109 RLRTV----------MTQPVAAAVVYVATEMAGPGHVKHHGRGELVIEPAAGSRDAADAL 158 ++ V + +V+++ E+ G ++ +V+ +G + ++ L Sbjct: 133 TVKAVDPEGALAAALPLAQIIGSVLFITAEVESAGVIRSVNPHLMVLGEPSG--EMSERL 190 Query: 159 VAV-------GVPTEISDNVRGELWLKLILNCAYNALSAIAQLPYGKLVEGPGIPEVIAQ 211 + V G+ +D +R +LW K+I N + N LS I Q +L P + EV++Q Sbjct: 191 LRVRAAVESAGIEARATDRIRDKLWTKIIANISTNPLSVITQGTLQQLYGLPELREVVSQ 250 Query: 212 LVTECKAVAAAEGVTLADDVDS-AVRKIVQTMPGQFSSTAQDLARGKPSEIDHLNGFIVA 270 ++ E VA++ G + D+D ++ + M +S QDL RG+P E+ + ++ Sbjct: 251 IMRETLLVASSHGARV--DIDPLTFLQLGEAMGAFRTSMLQDLERGRPLELAAIGDAVLE 308 Query: 271 RAQAHGLSAPANLTVHALVKL 291 A+ + + P + +L + Sbjct: 309 MAERYAIPMPITRAIVSLARF 329 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.131 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 907,599 Number of extensions: 36669 Number of successful extensions: 170 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 164 Number of HSP's successfully gapped: 3 Length of query: 297 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 212 Effective length of database: 1,217,395 Effective search space: 258087740 Effective search space used: 258087740 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)