BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0032.p (311 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311165|ref|YP_003775257.1| transcription regulator protein... 627 0.0 gi|300312614|ref|YP_003776706.1| LysR family transcription regul... 163 2e-41 gi|300311542|ref|YP_003775634.1| LysR family transcription regul... 85 9e-18 gi|300313217|ref|YP_003777309.1| LysR family transcription regul... 79 5e-16 gi|300310792|ref|YP_003774884.1| LysR family transcription regul... 76 4e-15 >gi|300311165|ref|YP_003775257.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 311 Score = 627 bits (1618), Expect = 0.0, Method: Compositional matrix adjust. Identities = 310/311 (99%), Positives = 310/311 (99%) Query: 1 MNIRFLETFVWLARLRNFRLTAERLHTTQAAVSSRIASLEQDFGVRLFDRSAREVALTAD 60 MNIRFLETFVWLARLRNFRLTAERLHTTQAAVSSRIASLEQDFGVRLFDRSAREVALTAD Sbjct: 1 MNIRFLETFVWLARLRNFRLTAERLHTTQAAVSSRIASLEQDFGVRLFDRSAREVALTAD 60 Query: 61 GSKALVHAERMVKLMREMKEDMSDREVYAGVIRIGVIESIVHSWFPDFLGRLHKTFPRLQ 120 GSKALVHAERMVKLMREMKEDMSDREVYAGVIRIGVIESIVHSWFPDFLGRLHKTFPRLQ Sbjct: 61 GSKALVHAERMVKLMREMKEDMSDREVYAGVIRIGVIESIVHSWFPDFLGRLHKTFPRLQ 120 Query: 121 IEIVSDTTIHLHEQFSKGNLDLVLQAEAVIGPQVSNLALCEFPMRWVGSPKLDIGSETLS 180 IEIVSDTTIHLHEQFSKGNLDLVLQAEAVIGPQVSNLALCEFPMRWVGSPKLDIGSETLS Sbjct: 121 IEIVSDTTIHLHEQFSKGNLDLVLQAEAVIGPQVSNLALCEFPMRWVGSPKLDIGSETLS 180 Query: 181 LADLAAFPIISFARNSGPHAVIERLFSDSQRSERTLHINCIASVATMIRLATDGFGITAV 240 LADLAAFPIISFARNSGPHAVIERLFSDSQRSERTLHINCIASVATMIRLATDGFGITAV Sbjct: 181 LADLAAFPIISFARNSGPHAVIERLFSDSQRSERTLHINCIASVATMIRLATDGFGITAV 240 Query: 241 PPAIIQRELNEESLHLLRVDTEFPALVLIASFRSDHENPLTETVARIAQETAADFALNWG 300 PPAIIQRELNEESLHLLRVDTEFPALVLIASFRSDHENPLTETVARIAQETAADFALNWG Sbjct: 241 PPAIIQRELNEESLHLLRVDTEFPALVLIASFRSDHENPLTETVARIAQETAADFALNWG 300 Query: 301 HEIARLPAPLN 311 HEIARLPA LN Sbjct: 301 HEIARLPASLN 311 >gi|300312614|ref|YP_003776706.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 301 Score = 163 bits (412), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 97/292 (33%), Positives = 160/292 (54%), Gaps = 3/292 (1%) Query: 1 MNIRFLETFVWLARLRNFRLTAERLHTTQAAVSSRIASLEQDFGVRLFDRSAREVALTAD 60 MN+RF+E F W A L++ AE+LH TQ+ +SSRIASLE++ GV L DR ++ LT Sbjct: 1 MNLRFVEAFHWAASLKSITRAAEKLHITQSTMSSRIASLEEELGVVLLDRREKQFRLTVA 60 Query: 61 GSKALVHAERMVKLMREMKEDMSDREVYAGVIRIGVIESIVHSWFPDFLGRLHKTFPRLQ 120 G + A +++ + R++++++ +RIG IES+VHSW P +L + +P Sbjct: 61 GQRFQRLAVKLLDMQRQIRQELGGHSAGPLALRIGSIESVVHSWLPAWLQEIRCRYPDFA 120 Query: 121 IEIVSDTTIHLHEQFSKGNLDLVLQAEAVIGPQVSNLALCEFPMRWVGSPKLDIGSETLS 180 +E+ +T+ L EQ +G DLV + V + + M +VG + G+ + + Sbjct: 121 LELTVETSPVLTEQIRRGAQDLVFTSTVGSDTAVRSRLMTPMEMVFVGH-RERFGTRSHT 179 Query: 181 LADLAAFPIISFARNSGPHAVIERLFSDSQRSERTLHINCIASVATMIRLATDGFGITAV 240 L +LAA +I+F R S PH + +L + + +H I+S++ M++L GFG+ + Sbjct: 180 LEELAAHDLITFQRGSQPHQGLLQLCQEWGVANPRIH--AISSISAMVQLVEGGFGVATL 237 Query: 241 PPAIIQRELNEESLHLLRVDTEFPALVLIASFRSDHENPLTETVARIAQETA 292 P A +++ L +LR + PAL + AS+R D + L E V A E A Sbjct: 238 PRASVRQLTRRLPLRILRCQAQLPALQIHASYREDPSSSLAELVLDSALEHA 289 >gi|300311542|ref|YP_003775634.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 335 Score = 84.7 bits (208), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 62/243 (25%), Positives = 107/243 (44%), Gaps = 5/243 (2%) Query: 1 MNIRFLETFVWLARLRNFRLTAERLHTTQAAVSSRIASLEQDFGVRLFDRSAREVALTAD 60 ++ R L+ F+ LA R+F A+R H +Q+A S+ I +E G RLF+R R V LT + Sbjct: 5 ISTRLLQAFLALADCRHFGHAAQRCHVSQSAFSAMIQKIEAAAGARLFERDTRNVTLTPE 64 Query: 61 GSKALVHAERMVKLMREMKEDMSDREV-YAGVIRIGVIESIVHSWFPDFLGRLHKTFPRL 119 G + A ++V + DMSD G + I + S+ W P L + +P + Sbjct: 65 GELFVQVARQLVSDIEAAFSDMSDYVARRKGRVAIAALPSLAAGWLPPVLADYRQRYPGV 124 Query: 120 QIEIVSDTTIHLHEQFSKGNLDLVLQAEAVIGPQVSNLALCEFPMRWVGSPKLDIGSE-T 178 +E+ + + G +D+ L A + LC P V + S+ Sbjct: 125 TVELFDAISDQCLDLLRHGKVDIALTAPGPNLLEFDTRPLCSDPFYLVCRKDHKLASKRR 184 Query: 179 LSLADLAAFPIISFARNSGPHAVIERLFSDSQRSERTLHINCIASVATMIRLATDGFGIT 238 + + LA +I AR++ ++ + L + +A+VA +I G G++ Sbjct: 185 IKVPQLAGCEMIHLARSTSVRQHLDAVLRPGGVIHTGLEVEHLATVAALIE---SGLGVS 241 Query: 239 AVP 241 VP Sbjct: 242 VVP 244 >gi|300313217|ref|YP_003777309.1| LysR family transcription regulator [Herbaspirillum seropedicae SmR1] Length = 296 Score = 79.0 bits (193), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 57/161 (35%), Positives = 81/161 (50%), Gaps = 10/161 (6%) Query: 1 MNIRFLETFVWLARLRNFRLT---------AERLHTTQAAVSSRIASLEQDFGVRLFDRS 51 MN LE + LA L FR AERL+ Q+ V++RI LEQ GV LF R Sbjct: 1 MNPSLLEIDMELAELEIFRTVVAEGGVTRAAERLNRVQSNVTTRIKQLEQSMGVPLFVRD 60 Query: 52 AREVALTADGSKALVHAERMVKLMREMKEDMSDREVYAGVIRIGVIESIVHSWFPDFLGR 111 R + LTA G L +AER++ L +E ++ ++ + G +RIG +ES S P L Sbjct: 61 RRRMVLTAAGEALLDYAERILDLAQEARQAVAPQSPR-GRLRIGSMESAAASRLPGPLAE 119 Query: 112 LHKTFPRLQIEIVSDTTIHLHEQFSKGNLDLVLQAEAVIGP 152 H+ +P +++E+ + T L Q LD L A V P Sbjct: 120 FHQRWPEVRLELSTAPTDVLMAQVRACKLDAALVAGPVSDP 160 >gi|300310792|ref|YP_003774884.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 302 Score = 75.9 bits (185), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 67/245 (27%), Positives = 117/245 (47%), Gaps = 18/245 (7%) Query: 6 LETFVWLARLRNFRLTAERLHTTQAAVSSRIASLEQDFGVRLFDRSAREVALTADGSKAL 65 L+ F+ +AR +FR AE L TQ A+S RI +LEQ RLFDR+ R V+LT G L Sbjct: 10 LQAFIAVARQSSFRAAAEGLFITQPALSRRIENLEQALQERLFDRTTRRVSLTPAGELFL 69 Query: 66 VHAERMVK----LMREMKEDMSDREVYAGVIRIGVIESIVHSWFPDFLGRLHKTFPRLQI 121 VHA+ +++ ++ +++ M+ R + + + + S+ ++ P L R ++ +++ Sbjct: 70 VHAQAVMEELELAVKSVEQRMAQRREH---VTVACVPSVANNLLPQVLKRYARSHANVRV 126 Query: 122 EIVSDTTIHLHEQFSKGNLDLVLQAEAVIGPQVSNLALCEFPM-RWVGSPKLD---IGSE 177 I+ ++ + E KG D + IG Q + + E R++ Sbjct: 127 RIIDESAADVLESVRKGEADFGVN---FIGAQEAEIDFAELTRERYLAVMPTHHPLAAHA 183 Query: 178 TLSLADLAAFPIISFARNSGPHAVIERLFSD-SQRSERTLHINCIASVATMIRLATDGFG 236 +L+ LA ++S + +SG ++I+ FS QR N VA I L G G Sbjct: 184 SLNWKSLAGEKLVSVSSSSGNRSLIDNAFSRVPQRPVIQYETN---HVAGAISLVAAGLG 240 Query: 237 ITAVP 241 + +P Sbjct: 241 VALLP 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 891,291 Number of extensions: 33943 Number of successful extensions: 215 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 208 Number of HSP's successfully gapped: 39 Length of query: 311 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 226 Effective length of database: 1,217,395 Effective search space: 275131270 Effective search space used: 275131270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)