BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0031.p (437 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 285 6e-78 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 178 7e-46 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 167 1e-42 gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 160 1e-40 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 150 1e-37 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 285 bits (728), Expect = 6e-78, Method: Compositional matrix adjust. Identities = 138/138 (100%), Positives = 138/138 (100%) Query: 1 MSYSPSAAATPAAHASAAAPLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 MSYSPSAAATPAAHASAAAPLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD Sbjct: 1 MSYSPSAAATPAAHASAAAPLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQD 60 Query: 61 LQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPT 120 LQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPT Sbjct: 61 LQFSEAVYGLGAGIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPT 120 Query: 121 SFYILRFLLGIFEAGFFP 138 SFYILRFLLGIFEAGFFP Sbjct: 121 SFYILRFLLGIFEAGFFP 138 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 178 bits (452), Expect = 7e-46, Method: Compositional matrix adjust. Identities = 80/117 (68%), Positives = 98/117 (83%) Query: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 + TY K+ WRL+P L +V +++DRVNVGFAKLQMLQDL+FS+ VYGLGAGIFF+GYF+ Sbjct: 18 DATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFV 77 Query: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFP 138 FEVPSN++L K+GAR +ARI I WG+ S AMAYVTTP FYI+RFLLG+ EAGFFP Sbjct: 78 FEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFP 134 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 167 bits (424), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 74/114 (64%), Positives = 94/114 (82%) Query: 25 YRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFIFEV 84 Y+K+ WR++PFL+ +V+A++DRVNVGFAKLQM QDL FSE V+GLGAG+FFIGYF+FEV Sbjct: 22 YKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFLFEV 81 Query: 85 PSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFP 138 PSN+L+ K+GAR +ARI I WG+ S A +V T FYILRFLLG+ EAGF+P Sbjct: 82 PSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYP 135 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 160 bits (406), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 70/117 (59%), Positives = 93/117 (79%) Query: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 + YRK+ R+IPFL +V++++DR+N+GFA+LQM QDL FS+A+YGLGA IF++GY + Sbjct: 22 DAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDLGFSDAMYGLGAAIFYVGYVL 81 Query: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFP 138 FEVPSN+ L + GAR+T +RI +LWGLAS+ M VT FY LRFLLG+FEAGFFP Sbjct: 82 FEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAAHFYTLRFLLGVFEAGFFP 138 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 150 bits (380), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 74/135 (54%), Positives = 96/135 (71%), Gaps = 9/135 (6%) Query: 13 AHASAAAPLEVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGA 72 A +A + Y+K+ W+++P+L+ +V+A++DRVNVGFAKL ML DL SEA YGLGA Sbjct: 4 ASQGSAVQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGA 63 Query: 73 GIFFIGYFIFEVPSNLLLEKIGARKTLARITILWGLASMAMAY---------VTTPTSFY 123 GIFF+GYFIFEVPSNL+L ++GA+ +ARI I WG+ S MA+ V+ T FY Sbjct: 64 GIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVFY 123 Query: 124 ILRFLLGIFEAGFFP 138 LRFLLG EAGFFP Sbjct: 124 TLRFLLGASEAGFFP 138 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.333 0.139 0.522 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 971,566 Number of extensions: 32976 Number of successful extensions: 229 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 230 Number of HSP's successfully gapped: 11 Length of query: 437 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 349 Effective length of database: 1,203,190 Effective search space: 419913310 Effective search space used: 419913310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 132 (55.5 bits)