BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0029.p (450 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 890 0.0 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 145 7e-36 gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 121 9e-29 gi|300310266|ref|YP_003774358.1| amidase family protein [Herbasp... 106 5e-24 gi|300310674|ref|YP_003774766.1| amidase family protein [Herbasp... 99 7e-22 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 890 bits (2301), Expect = 0.0, Method: Compositional matrix adjust. Identities = 448/450 (99%), Positives = 448/450 (99%) Query: 1 MPSTLSRLAADLAAGHTTSLQLTEQALARIADPAGEGAHVFTRVYAEQARAAARASDTLR 60 MPSTLSRLAADLAAGHTTSLQLTEQALARIADPAGEGA VFTRVYAEQARAAARASDTLR Sbjct: 1 MPSTLSRLAADLAAGHTTSLQLTEQALARIADPAGEGARVFTRVYAEQARAAARASDTLR 60 Query: 61 AAGLCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGK 120 AA LCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGK Sbjct: 61 AARLCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGK 120 Query: 121 TNMTEFAYSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGG 180 TNMTEFAYSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGG Sbjct: 121 TNMTEFAYSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGG 180 Query: 181 SVRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGEDYVA 240 SVRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGEDYVA Sbjct: 181 SVRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGEDYVA 240 Query: 241 VLAHPLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVPAFNALAAINA 300 VLAHPLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVPAFNALAAINA Sbjct: 241 VLAHPLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVPAFNALAAINA 300 Query: 301 KGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAQVEAQ 360 KGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAQVEAQ Sbjct: 301 KGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARPRWIAQVEAQ 360 Query: 361 LAGYDALVMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLDGCALSLPCHAPGS 420 LAGYDALVMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLDGCALSLPCHAPGS Sbjct: 361 LAGYDALVMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLDGCALSLPCHAPGS 420 Query: 421 APVGLSLAACGGQDKRLLSIGLAVEALLAA 450 APVGLSLAACGGQDKRLLSIGLAVEALLAA Sbjct: 421 APVGLSLAACGGQDKRLLSIGLAVEALLAA 450 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 145 bits (366), Expect = 7e-36, Method: Compositional matrix adjust. Identities = 143/432 (33%), Positives = 208/432 (48%), Gaps = 52/432 (12%) Query: 47 EQARAAARASDTLRAAGLCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAV 106 EQ R+ AR D G PL G+P+++KD DVAG + AG+ LR V HA V Sbjct: 48 EQLRSQARMLDEQGGHG----PLSGIPIAIKDNIDVAGIASTAGTAALRNN-VPQRHATV 102 Query: 107 VQNLLKAGAILIGKTNMTEFAYSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVT 166 V+ L AGA+ GK NM E A+ N +G +N ++ + I GGSS G + Sbjct: 103 VEYLFGAGALHAGKANMHELAFGITNNNGVFGPCRNPYDAEL----IAGGSSGGCGALLA 158 Query: 167 DGMAFAAVGSDTGGSVRIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCC 226 +A AAVG+DTGGSVRIP+AL G+ G +PT+ R S GV+P+S D+ GP+A SV Sbjct: 159 ARLAPAAVGTDTGGSVRIPAALCGVVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADV 218 Query: 227 AIMDAVLAG-EDYVAVLAHPLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIV 285 A++D ++AG D +LA P LRL N G+++ V AL +L AG +V Sbjct: 219 ALLDQIMAGSRDSAPLLALPPSQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELV 278 Query: 286 EAEVPAFNALAAINAKGGFTAAEAYAWHRALI---AENAAGYDQRVVSRILRGKDMSAAD 342 + ++P LA ++ GF A H LI A A + V+ R+L ++ + D Sbjct: 279 DVDLP---TLAPLSHAAGF----AIVLHEFLIDLPAYLQASDARDVIKRVL--AEVGSPD 329 Query: 343 LLDVL----------HARPRWIAQVEAQLA---------GYDALVMPTVPIVAPRIAELQ 383 + ++ HA + Q A G DAL+ PT + A + E Sbjct: 330 VARIIQATLDQPVPEHAYRLALEQRRTMQALYRDCFRSQGVDALIFPTTALTARPLGE-- 387 Query: 384 ASDDAYYGANGKMLRNPTLINFLDGC------ALSLPCH-APGSAPVGLSLAACGGQDKR 436 DD ++ P DG ++S+P + PVGL L G D+R Sbjct: 388 --DDTVALRGERVPTFPAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRR 445 Query: 437 LLSIGLAVEALL 448 LL + ++E +L Sbjct: 446 LLQVAASLETVL 457 >gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 488 Score = 121 bits (304), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 140/481 (29%), Positives = 213/481 (44%), Gaps = 57/481 (11%) Query: 4 TLSRLAADLAAGHTTSLQLTEQALARIADPAGEGAHVFTRVYAEQARAAARASDTLRAAG 63 TL L+A L A ++ +L L RI + F V E A A+ +D+ R + Sbjct: 5 TLKELSAQLQARKISATELATLFLDRID---ASDLNAFLHVDRELTLAQAKVADS-RLSD 60 Query: 64 LCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNM 123 L GVP++ KD+F G T AGS +L V+ A VV+ AG + +GK N Sbjct: 61 NNAGQLTGVPIAHKDIFVTRGWRTTAGSRMLDNY-VSPFDATVVEQFNAAGMVTLGKLNC 119 Query: 124 TEFAYSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVR 183 EFA N ++G N W++ G SS G+A +V +A AA +DTGGS+R Sbjct: 120 DEFAMGSGNENSYFGPTLNPWDKRAVPGG----SSGGSAAAVAARLAPAATATDTGGSIR 175 Query: 184 IPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAG-------- 235 P++L G+TG KPT VS G++ ++ LD GP+A + + C ++ + G Sbjct: 176 QPASLCGVTGIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTS 235 Query: 236 -----EDYVAVLAHPLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEA--- 287 ED+ L L+GLR+ P G+ V A R AL + GA +VE Sbjct: 236 LQRPKEDFTRSLNDSLQGLRIGVPKEFFGAGLAADVEAAVRAALSEFEKLGAQLVEISLP 295 Query: 288 ----EVPAFNALAAINAKGGFTAAEAYAW-HRALIAEN---------AAGYDQRVVSRIL 333 +P + +A A + + + HRA N A G+ + V RIL Sbjct: 296 KTELSIPVYYVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAEGFGEEVKRRIL 355 Query: 334 RGKDMSAADLLDVLHARPRWIAQVEAQ-----LAG----YDALVMPTVPIVAPRIAELQA 384 G + + D + + + I ++ AQ LAG D ++ P P VA + + Sbjct: 356 VGSYVLSHGYYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPVSPTVAWDLGDKTN 415 Query: 385 SDDAYYGANGKMLRNPTLINFLDGCALSLPC-----HAPGSAPVGLSLAACGGQDKRLLS 439 A Y A+ + T + L G +S+PC G PVGL + + RLL+ Sbjct: 416 DPVANYLAD--IFTLSTSLAGLPG--MSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLN 471 Query: 440 I 440 + Sbjct: 472 V 472 >gi|300310266|ref|YP_003774358.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 106 bits (264), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 125/421 (29%), Positives = 170/421 (40%), Gaps = 42/421 (9%) Query: 63 GLCRSPLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTN 122 G L G V +KD +AG + GS L G V S A +V LL AGA ++GK Sbjct: 82 GAAEGKLAGRTVVLKDNVALAGVPMMNGSSTLEGF-VPSYDATIVTRLLDAGATILGKAT 140 Query: 123 MTEFAYSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSV 182 F SG H P G G SS AA+ V G A A+G D GGS+ Sbjct: 141 CEHFCLSG---GSHTSDPAPVHNPRRHGYGSGGSSSGSAAL-VAAGEADMAIGGDQGGSI 196 Query: 183 RIPSALNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQCCAIMDAVLAGED----- 237 RIPSA G G K T V GV+P+ A +D +GP+ +V+ A+M V+AG D Sbjct: 197 RIPSAFCGTYGMKATHGLVPYTGVMPIEATVDHVGPITANVRDNALMLEVIAGADGLDPR 256 Query: 238 --------YVAVLAHPLRGLRL-LAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAE 288 Y A L +RGL++ + L MD V + A+ +L GA + Sbjct: 257 QYAPEVQPYTAALDRGVRGLKIGILTEGFALGNMDKRVADKVQEAIAQLEKLGASVATVS 316 Query: 289 VPAFNALAAINAKGGFTAAEAYAWHRALIAENAAG-YD----QRVVSRILRGKDMSAADL 343 V A+ G H + N G YD Q D+SA Sbjct: 317 VEEHALAGALWQPIGCEGLTMQMMHGNGMGFNWKGLYDLGLLQHHAHWREHADDLSATLK 376 Query: 344 LDV-------------LHARPRWIAQ-----VEAQLAGYDALVMPTVPIVAPRIAELQAS 385 L + +A+ + IA+ + LA +D LVMPTVPI+A + Sbjct: 377 LCMFVGQYGLTRYQGRFYAKAQNIARRARAGYDKALADFDLLVMPTVPIIAQPLPAAGCP 436 Query: 386 DDAYYGANGKMLRNPTLINFLDGCALSLPCHAPGSAPVGLSLAACGGQDKRLLSIGLAVE 445 Y +M+ N ++ A+S+PC PVGL L + L A E Sbjct: 437 LAEYVARAFEMIGNTAPLDITGHPAMSIPCGTVDGLPVGLMLVGKHYAEATLYQAAAAFE 496 Query: 446 A 446 A Sbjct: 497 A 497 >gi|300310674|ref|YP_003774766.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 99.0 bits (245), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 64/153 (41%), Positives = 85/153 (55%), Gaps = 4/153 (2%) Query: 71 GVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNMTEFAYSG 130 G V++KD D+AG+ T GS L P ASAHA VV LL+AG + + NM E AY Sbjct: 26 GPTVAIKDCIDIAGQPTCGGSAALADAPPASAHADVVARLLEAGWQITARANMHELAYGM 85 Query: 131 LGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSALNG 190 GIN GT N + G GS+S + D ++GSDTGGS+R+P+A G Sbjct: 86 TGINEWNGTAVNPQDPACMTGGSSSGSASAVGAGLVD----VSIGSDTGGSIRLPAACCG 141 Query: 191 LTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSV 223 + GFKP+ RVS G P + LD +GP + S+ Sbjct: 142 VIGFKPSFGRVSRAGAYPALSSLDCLGPFSRSM 174 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,411,490 Number of extensions: 59276 Number of successful extensions: 278 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 253 Number of HSP's successfully gapped: 11 Length of query: 450 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 362 Effective length of database: 1,203,190 Effective search space: 435554780 Effective search space used: 435554780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)