BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0021.p (483 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 832 0.0 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 394 e-111 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 356 2e-99 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 351 9e-98 gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipo... 267 2e-72 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 832 bits (2150), Expect = 0.0, Method: Compositional matrix adjust. Identities = 449/458 (98%), Positives = 449/458 (98%), Gaps = 8/458 (1%) Query: 23 TACQSLAPHYQRPAAPVAAVYPDGPVNEAP--------SPPLPWRDYFTDPPLQALITQA 74 TACQSLAPHYQRPAAPVAAVYPDGPVNEA SPPLPWRDYFTDPPLQALITQA Sbjct: 23 TACQSLAPHYQRPAAPVAAVYPDGPVNEAAHQVANEAASPPLPWRDYFTDPPLQALITQA 82 Query: 75 LENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQ 134 LENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQ Sbjct: 83 LENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQ 142 Query: 135 AGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLE 194 AGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLE Sbjct: 143 AGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLE 202 Query: 195 IANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTL 254 IANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTL Sbjct: 203 IANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTL 262 Query: 255 LVGSPVVLPVGQARAGLDAVFAPLQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAAF 314 LVGSPVVLPVGQARAGLDAVFAPLQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAAF Sbjct: 263 LVGSPVVLPVGQARAGLDAVFAPLQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAAF 322 Query: 315 FPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVA 374 FPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVA Sbjct: 323 FPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVA 382 Query: 375 AYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLD 434 AYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLD Sbjct: 383 AYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLD 442 Query: 435 AQRDLLSAQQQLVQIRRALLSSSVALYAALGGEGVAAV 472 AQRDLLSAQQQLVQIRRALLSSSVALYAALGGEGVAAV Sbjct: 443 AQRDLLSAQQQLVQIRRALLSSSVALYAALGGEGVAAV 480 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 394 bits (1012), Expect = e-111, Method: Compositional matrix adjust. Identities = 218/453 (48%), Positives = 302/453 (66%), Gaps = 5/453 (1%) Query: 17 VLPLCLTACQSLAPHYQRPAAPVAAVYPDGPVNEAP--SPPLPWRDYFTDPPLQALITQA 74 V+ L C SLAP Y+RP APV + YP A + L WR +F D LQ LI A Sbjct: 20 VMAGVLGGC-SLAPTYERPQAPVESSYPADAAGRAELQAVNLGWRQFFPDQRLQQLIAAA 78 Query: 75 LENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSAYQ 134 LEN+RDLRTA L ++EA+A Y I ADL P + R+RT A +LT +P + ++YQ Sbjct: 79 LENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQPTISNSYQ 138 Query: 135 AGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDERLE 194 GL + ++E+D +GR+R+L DAAL S +++ + + +SL+AQVA A+L E+L+ Sbjct: 139 VGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAERSYAEQLK 198 Query: 195 IANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNALTL 254 +A++T+ SRQ + + +R++VGA++ L+ +TL+ A+ QL++QRAQ NALTL Sbjct: 199 LADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESARVAKTQLERQRAQAENALTL 258 Query: 255 LVGSPV-VLPVGQARAGLDAVFAPLQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAA 313 LVG + LP Q + + + + GLP+ LLT RPD+ +AE L+AANANIGAARAA Sbjct: 259 LVGKKIGELPPAQLLSD-EKIVTDIPAGLPSDLLTNRPDIRSAEQSLLAANANIGAARAA 317 Query: 314 FFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAV 373 FFP ISLTGN G+AS+ L GLF GS AW+F PS++LPIF GR NL L+EVR++ AV Sbjct: 318 FFPRISLTGNFGSASSTLGGLFESGSGAWSFGPSLSLPIFDFGRNSANLDLAEVRKNKAV 377 Query: 374 AAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVL 433 YEK +QTAFREVADAL AR L +Q++ ++ + Q+ER +L+ LR+ NG A LD L Sbjct: 378 VTYEKAIQTAFREVADALVARGSLDEQVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQL 437 Query: 434 DAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 466 DAQRDL SAQQ L+Q R+ L++++ LY ALGG Sbjct: 438 DAQRDLFSAQQALIQARQLRLNNAIDLYKALGG 470 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 356 bits (914), Expect = 2e-99, Method: Compositional matrix adjust. Identities = 218/489 (44%), Positives = 297/489 (60%), Gaps = 25/489 (5%) Query: 7 LLTRTRLLLAVLP----LCLTACQSLAPHYQRPAAPVAAVYPDGPVNEA---PSPP---- 55 L TR+ +L +LP + L C +L P Y RP APVAA + A S P Sbjct: 5 LSTRSPVLPMMLPVMLAMLLGGC-TLMPDYHRPDAPVAAHFAGADSKNAAGASSAPVVAD 63 Query: 56 LPWRDYFTDPPLQALITQALENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRS 115 + WRD FTDP LQ +I ALEN+RDLR A L +++A+A YG+ A LFP+I A S Sbjct: 64 IGWRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETAS 123 Query: 116 RTPADLNLTRRPLVGSAYQAGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSL 175 RTP DL+ T L +Y A LG S++E+DL+GR+RSL + AL+S ++ R + +SL Sbjct: 124 RTPGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISL 183 Query: 176 MAQVADAWLELAELDERLEIANQTIASRQESFRIFSRRVEVGATSRLNL----TQIETLL 231 +A+VA AWL LA +RL +A T+ S+ S+ + R E+G++S L L T +E+ Sbjct: 184 IAEVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESAR 243 Query: 232 TQAQALGAQLQQQRAQRLNALTLLVGSPV---VLPVG--QARAGLDAVFAPLQPGLPALL 286 ++ AQ+ Q R NAL LLVG + +LP G R + A + PGLP+ L Sbjct: 244 VDVESYTAQVDQDR----NALVLLVGKDLPTALLPQGLPDTRLAAASPLATIPPGLPSDL 299 Query: 287 LTRRPDVAAAEHRLMAANANIGAARAAFFPSISLTGNVGTASAELSGLFRDGSHAWTFSP 346 L RR D+ AE L AANANIGAARAAF+PSI+LT N GTASA L+GLF+ GS +W+F P Sbjct: 300 LQRRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLAGLFKGGSGSWSFVP 359 Query: 347 SITLPIFTAGRLRNNLALSEVRRDLAVAAYEKTVQTAFREVADALAARQWLTQQLEIAQS 406 I+LPIF G + NL ++ V RD++VA YEK +QTAFREV+DALA R L +QL+ ++ Sbjct: 360 QISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSDALAQRNTLGRQLQAQEA 419 Query: 407 AVRVQRERARLSALRYDNGAAPFLDVLDAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 466 V+ E RLS RY +G +L VLD+QR L SAQ+ L+ + + ++ V Y LGG Sbjct: 420 LVQASDEAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKNLIGTQLSRWTNLVTFYKTLGG 479 Query: 467 EGVAAVPSP 475 V +P Sbjct: 480 GWVERTGTP 488 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 351 bits (900), Expect = 9e-98, Method: Compositional matrix adjust. Identities = 213/458 (46%), Positives = 280/458 (61%), Gaps = 19/458 (4%) Query: 25 CQSLAPHYQRPAAPVAAVYPD-----------GPV-NEAPSPPLPWRDYFTDPPLQALIT 72 C +LAP Y +PAAPV A + GP E + L WR++F DP LQ +I Sbjct: 28 CANLAPDYTQPAAPVPASWTSQAAAQPAVGSAGPAAGEIDADHLGWREFFLDPRLQQVIA 87 Query: 73 QALENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADLNLTRRPLVGSA 132 AL+N+RDLR AAL +++A+A Y I A+ +P+I A SRTPA+L+ T V Sbjct: 88 AALDNNRDLRVAALNIEKARAQYRITDANRYPSISATGTSSASRTPAELSTTGSAAVSHQ 147 Query: 133 YQAGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVADAWLELAELDER 192 +G+S++E+D +GRL++L DAALES + TR + +SL+A+VA AWL LA + Sbjct: 148 QSVTVGVSAYELDFFGRLKNLSDAALESYQYQVETRRSTHISLVAEVATAWLTLASDRDL 207 Query: 193 LEIANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQLQQQRAQRLNAL 252 L++A T AS+ +F + R ++G S ++L E + A+A A+ Q Q LNAL Sbjct: 208 LKLAQDTYASQSRTFALSQRSHDIGTVSGVDLASQEATVESARADVARYASQVEQDLNAL 267 Query: 253 TLLVGSPV---VLPVGQARAGLDAVFAPLQP-GLPALLLTRRPDVAAAEHRLMAANANIG 308 LLVG+ + +LP G A P P GLP+ +L RRPDV AAEH L +ANA+IG Sbjct: 268 RLLVGADIDAALLPAGLPDT---ASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIG 324 Query: 309 AARAAFFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVR 368 AARAAFFPSISLT N GTAS L GLF+ GS +WTFSPSI LPIF AG LR +L +++ Sbjct: 325 AARAAFFPSISLTANGGTASRTLGGLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKIT 384 Query: 369 RDLAVAAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAP 428 RD+ VA YEK +QTAFREVADALA R L Q+L+ A + RLS RY NG Sbjct: 385 RDIDVANYEKAIQTAFREVADALAVRATLDQRLDAQGKATAASEKAFRLSDARYRNGVDS 444 Query: 429 FLDVLDAQRDLLSAQQQLVQIRRALLSSSVALYAALGG 466 +L L AQR L SAQQ L+ +R S+ + LY LGG Sbjct: 445 YLTTLVAQRTLYSAQQTLISLRLTEQSNRITLYKVLGG 482 >gi|300311271|ref|YP_003775363.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 481 Score = 267 bits (682), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 195/482 (40%), Positives = 263/482 (54%), Gaps = 20/482 (4%) Query: 8 LTRTRLLLAVLPLCLTACQSLAPHYQRPAAPVAAVYP-----DGPVNEAPSPP-LPWRDY 61 ++R LL L LC AC L A + YP D P N AP+ + W+DY Sbjct: 5 MSRHLRLLPTLALC--ACAPLTAPLPPSALDLPTHYPRTLAEDAPANAAPTASAIGWQDY 62 Query: 62 FTDPPLQALITQALENSRDLRTAALRVQEAQALYGIGHADLFPTIGAQAGLDRSRTPADL 121 F PLQALI +AL ++ DLR LR+Q+ QA + I + PT+ +A RSR P + Sbjct: 63 FPQAPLQALIAEALRHNPDLRDIRLRLQQVQAQWRIQRSTGLPTVNLEAQARRSRGPLEP 122 Query: 122 NLTRRPLVGSAYQAGLGLSSWEIDLWGRLRSLDDAALESARASQATRSAVALSLMAQVAD 181 + Y+ GLG SSWE+D WGR+ SL++AAL+ AS+A A+ +SL+ QVA Sbjct: 123 PESGPAASTREYRVGLGASSWELDFWGRIASLNEAALQEWMASRAAEQALVISLIGQVAA 182 Query: 182 AWLELAELDERLEIANQTIASRQESFRIFSRRVEVGATSRLNLTQIETLLTQAQALGAQL 241 AW L ELDERL + A+ ES + R + G L Q + L Q +A A+L Sbjct: 183 AWTSLRELDERLHLLETLQAATAESLALMQARHDAGRADAAALAQQRSELAQMRAEQAEL 242 Query: 242 QQQRAQRLNALTLLVGSPVVLPVGQARAGLD----AVFAP--LQPGLPALLLTRRPDVAA 295 QQ RA + + L +L G + Q A L+ + P L PGLP+ LL RPD+ A Sbjct: 243 QQMRASQRSTLEMLAG------LTQLPAALEPAPTTILLPFALAPGLPSTLLAARPDIIA 296 Query: 296 AEHRLMAANANIGAARAAFFPSISLTGNVGTASAELSGLFRDGSHAWTFSPSITLPIFTA 355 AEHRL A+ A + AARAAFFP ISLT G AS L LF+ S AW F P I+LP+ Sbjct: 297 AEHRLQASAAQVAAARAAFFPRISLTAFGGVASTALQDLFKGPSRAWNFVPEISLPLLDG 356 Query: 356 GRLRNNLALSEVRRDLAVAAYEKTVQTAFREVADALAARQWLTQQLEIAQSAVRVQRERA 415 G ++ ++ + + A+ YE+TVQ AFREVA L WL +QL + + V+ Q +R Sbjct: 357 GLRQSRYDIAALDQQRALLDYERTVQNAFREVAQRLEEAHWLKEQLRLRREVVQQQAQRQ 416 Query: 416 RLSALRYDNGAAPFLDVLDAQRDLLSAQQQLVQIRRALLSSSVALYAALGGEGVAAVPSP 475 +R G A LDVL+++RD L AQQ+ + ALL + +ALY ALGG A S Sbjct: 417 AQMHMRLRYGRASRLDVLESERDHLRAQQRQHMLEHALLRNQIALYVALGGGTQALSASS 476 Query: 476 PS 477 PS Sbjct: 477 PS 478 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,127,629 Number of extensions: 42352 Number of successful extensions: 268 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 205 Number of HSP's successfully gapped: 18 Length of query: 483 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 394 Effective length of database: 1,198,455 Effective search space: 472191270 Effective search space used: 472191270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)