BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0037.0003.p (355 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspi... 627 0.0 gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspi... 126 3e-30 gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspi... 119 3e-28 gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspi... 108 7e-25 >gi|300311135|ref|YP_003775227.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 351 Score = 627 bits (1616), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/355 (98%), Positives = 348/355 (98%), Gaps = 4/355 (1%) Query: 1 MSHAARTLLPSLADIEQAAGIVYAGMAPTAQLCWPLLNQALGAEVWVKHENHAPTGAFKV 60 MSHAARTLLPSL DIEQAAGIVYAGMAPTAQLCWPLLNQALGAEVWVKHENHAPTGAFKV Sbjct: 1 MSHAARTLLPSLTDIEQAAGIVYAGMAPTAQLCWPLLNQALGAEVWVKHENHAPTGAFKV 60 Query: 61 RGGMVYLHHLARQQPQLSGVVSATRGNHGQSVGLAAALAARRLGISATIVVPQGNSREKN 120 RGGMVYLHHLARQQPQLSGVVSATRGNHGQSVGLAA RRLGISATIVVPQGNSREKN Sbjct: 61 RGGMVYLHHLARQQPQLSGVVSATRGNHGQSVGLAA----RRLGISATIVVPQGNSREKN 116 Query: 121 AAMRALGVELIEHGSEFQESREYAQQLAAQQQLHMIPSLHRDLVAGVASYWMELFAAQPG 180 AAMRALGVELIEHGSEFQESREYAQQLAAQQQLHMIPSLHRDLVAGVASYWMELFAAQPG Sbjct: 117 AAMRALGVELIEHGSEFQESREYAQQLAAQQQLHMIPSLHRDLVAGVASYWMELFAAQPG 176 Query: 181 LDVVLVPIGQGSGMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPVSTCIA 240 LDVVLVPIGQGSGMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPVSTCIA Sbjct: 177 LDVVLVPIGQGSGMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPVSTCIA 236 Query: 241 DGVACRVPDQASLEVLFSEVDEVVAVTDDEVMDAMKLAYIATHNVAEGAGACALAAAWQL 300 DGVACRVPDQASLEVLFSEVDEVVAVTDDEVMDAMKLAYIATHNVAEGAGACALAAAWQL Sbjct: 237 DGVACRVPDQASLEVLFSEVDEVVAVTDDEVMDAMKLAYIATHNVAEGAGACALAAAWQL 296 Query: 301 RARLRGLKVGVTLSGGNVDHDVLARVLARAAAPADMLLAPAQVLPLASSPQRRSA 355 RARLRGLKVGVTLSGGNVDHDVLARVLARAAAPADMLLAPAQVLPL S PQRRSA Sbjct: 297 RARLRGLKVGVTLSGGNVDHDVLARVLARAAAPADMLLAPAQVLPLTSLPQRRSA 351 >gi|300309629|ref|YP_003773721.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 126 bits (316), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 111/324 (34%), Positives = 161/324 (49%), Gaps = 8/324 (2%) Query: 7 TLLPSLADIEQAAGIVYAGMAPTAQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVY 66 T LPS AD+ AA + T L + LGA+++ K EN GAFK RG M Sbjct: 8 TELPSFADVLLAAERIKPWAHRTPVLRSRTADTELGAQLFFKCENFQRMGAFKFRGAMNA 67 Query: 67 LHHLARQQPQLSGVVSATRGNHGQSVGLAAALAARRLGISATIVVPQGNSREKNAAMRAL 126 L Q + GV++ + GNH Q LAA L LGI A IV+PQ K AA R Sbjct: 68 LTQFDATQRK-HGVITFSSGNHAQGTALAAQL----LGIPAVIVMPQDAPAAKVAATRGY 122 Query: 127 GVELIEHGSEFQESRE-YAQQLAAQQQLHMIPSL-HRDLVAGVASYWMELFAAQPGLDVV 184 G E++ + + E RE +LAA++++ +IP H ++AG + +EL LD + Sbjct: 123 GAEVVTY-DRYTEDREAIGARLAAERKMTLIPPYDHPHVMAGQGTATLELLQETGPLDAL 181 Query: 185 LVPIGQGSGMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPVSTCIADGVA 244 V +G G + GA A AL + +I GV + S AG+ + IADG Sbjct: 182 FVCLGGGGLLSGAALAARALSPACKIYGVEPQAGNDGQQSLRAGQVVHIDTPKTIADGAQ 241 Query: 245 CRVPDQASLEVLFSEVDEVVAVTDDEVMDAMKLAYIATHNVAEGAGACALAAAWQLRARL 304 + + + V+ VD++ V+D+E++ M+ V E G AAA +L+ +L Sbjct: 242 TQHLGKYTFGVIRELVDDIHTVSDEELVAGMRFFAERMKMVVEPTGCLGFAAARKLKDQL 301 Query: 305 RGLKVGVTLSGGNVDHDVLARVLA 328 +G +VGV +SGGNVD LA LA Sbjct: 302 QGKRVGVIISGGNVDLGKLATFLA 325 >gi|300309398|ref|YP_003773490.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 401 Score = 119 bits (299), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 105/331 (31%), Positives = 167/331 (50%), Gaps = 9/331 (2%) Query: 9 LPSLADIEQAAGIVYAGMAPTAQLCWPLLNQALGAEVWVKHENHAPTGAFKVRGGMVYLH 68 + +L DIE AA + + T + L+Q +GA+V++K EN T +FK RG L Sbjct: 1 MTTLNDIEAAARRLQGQILETPCVESRTLSQIVGAQVFLKFENLQFTASFKERGACNKLV 60 Query: 69 HLARQQPQLSGVVSATRGNHGQSVGLAAALAARRLGISATIVVPQGNSREKNAAMRALGV 128 +A GVV+ + GNH Q V A A+RLG+ ATIV+P+ K R G Sbjct: 61 DVAASGGGARGVVAMSAGNHAQGV----AYHAQRLGLPATIVMPRFTPGVKIERTRGFGA 116 Query: 129 ELIEHGSEFQESREYAQQLAAQQQLHMI-PSLHRDLVAGVASYWMELFAAQPGLDVVLVP 187 ++ HG + +R +A +LA ++ L + P ++AG + +E+ A P LD ++V Sbjct: 117 NVVLHGDTLEAARTHAYELAEKEGLTFVHPYDDEAIIAGQGTVALEMMKAVPDLDCLVVS 176 Query: 188 IGQGSGMCGAVAARNALGLSTRIIGVVSAHALAYKLSFEAGRKIESPV-STCIADGVACR 246 IG G + G A A+ I+GV + + A + E PV ++ IA+G+A Sbjct: 177 IGGGGLLAGMAVAARAIKPEIEIVGVQTRR---FPTMVNAVKGTEHPVGASTIAEGIAVG 233 Query: 247 VPDQASLEVLFSEVDEVVAVTDDEVMDAMKLAYIATHNVAEGAGACALAAAWQLRARLRG 306 P + ++ E DE++ V + ++ A+ + + EGAGA LAA + R RG Sbjct: 234 TPGTLTRAIIAREADELLLVDEGDIEQAVLMLLEIEKTLVEGAGAAGLAALVRYPDRFRG 293 Query: 307 LKVGVTLSGGNVDHDVLARVLARAAAPADML 337 +VG+ LSGGN+D +L ++ R A L Sbjct: 294 KRVGLVLSGGNIDPLLLTAIIERGMVRAGRL 324 >gi|300313197|ref|YP_003777289.1| threonine dehydratase [Herbaspirillum seropedicae SmR1] Length = 508 Score = 108 bits (270), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 90/296 (30%), Positives = 148/296 (50%), Gaps = 14/296 (4%) Query: 37 LNQALGAEVWVKHENHAPTGAFKVRGGMVYLHHLARQQPQLSGVVSATRGNHGQSVGLAA 96 L+Q + ++ K E+ +FK+RG + HL Q + GV+ A+ GNH Q V L+A Sbjct: 29 LSQRIDNRIYFKREDMQSVFSFKLRGAYNKMAHLTPAQLK-RGVICASAGNHAQGVALSA 87 Query: 97 ALAARRLGISATIVVPQGNSREKNAAMRALGVELIEHGSEFQESREYAQQLAAQQQLHMI 156 A RLG A IV+P + K A++A G E++ G F ++ E+A L +Q+L + Sbjct: 88 A----RLGCRAVIVMPTTTPQVKIDAVKARGGEVVLFGDSFTDAYEHALTLEKKQKLTFV 143 Query: 157 -PSLHRDLVAGVASYWMELFAAQPG-LDVVLVPIGQGSGMCGAVAARNALGLSTRIIGVV 214 P ++AG + ME+ P + + V IG G + G + A+ +IIGV Sbjct: 144 HPFDDPYVIAGQGTVGMEILRQHPDPIHAIFVAIGGGGLIAGVASYVKAVRPDIKIIGVQ 203 Query: 215 SAHALAYKLSFEAGRKIESPVSTCIADGVACRVPDQASLEVLFSEVDEVVAVTDDEVMDA 274 + + A S +AGR++ P +DG A ++ + + + VDEV+ V D V A Sbjct: 204 TTDSDAMARSLKAGRRVALPDVGLFSDGTAVKLVGEETFRIAKELVDEVIIVDTDAVCTA 263 Query: 275 MKLAYIATHNVAEGAGACALAA--AWQLRAR-----LRGLKVGVTLSGGNVDHDVL 323 +K + T ++ E AGA A+A A+ RA+ ++G + G N++ D L Sbjct: 264 IKDIFQDTRSIVEPAGALAVAGAKAYVERAKASKKPIKGESLITIACGANMNFDRL 319 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.131 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 908,878 Number of extensions: 35258 Number of successful extensions: 149 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 4 Length of query: 355 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 269 Effective length of database: 1,212,660 Effective search space: 326205540 Effective search space used: 326205540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)