BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0130.p (408 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313991|ref|YP_003778083.1| major facilitator superfamily p... 104 1e-23 gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance... 80 3e-16 gi|300311040|ref|YP_003775132.1| major facilitator superfamily p... 60 4e-10 gi|300313188|ref|YP_003777280.1| major facilitator superfamily p... 57 2e-09 >gi|300313991|ref|YP_003778083.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 406 Score = 104 bits (259), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 107/373 (28%), Positives = 173/373 (46%), Gaps = 24/373 (6%) Query: 18 PAWRMMMLCCLIVFMAQMATTLYLPSLPIVMRELAMSQSLVEMSISGFVVGAALPVLFWG 77 PAW + + + M +A +YLPS + ++L + + V+++++ F++G A+ +F+G Sbjct: 14 PAW--LAVLAGLTAMGALAIDMYLPSFTAIAQDLGVGTNQVQLTLATFLIGVAVGQMFYG 71 Query: 78 TAADRWGRRGPLLASLALFVLCSALLAAVGSAVELLFLRVLQGIAAGGAAIIARIIVRDN 137 +DR+GR+ PL +AL+ +CS L + L+ LR+ QG+ +I R +VRD Sbjct: 72 PLSDRFGRKPPLFFGIALYFVCSLLCIFAQNIETLIVLRLFQGLGGSAGMVIPRAMVRDR 131 Query: 138 WSGDELARRLSVLSIAFITALGAGQFIGGLIGQYSHWQIGFVLMAVTGALAAL------- 190 + A+ S+L + F A FIGGL+ ++ W+ FV++ + G L L Sbjct: 132 MGAEGSAKAFSMLMLVFGLAPILAPFIGGLMLVFASWRGIFVVLTLFGLLCILGTRKYLT 191 Query: 191 ----LSMTLPLQGGRQAAARQGGNPYWQILRRPGFVRPACAGGLGFATTITLQEASPFVF 246 +S PL GR RQ YW +LR F+ GGL A PFV Sbjct: 192 ETVDVSRAEPLHLGR--TLRQ----YWGLLRHRQFMSYVLCGGLVQAGMFAYIAGGPFVV 245 Query: 247 QEHFGLAVASFGNVGLLIGVAYFLGAWTVNRTVSKLGGKKLMCAGALLIAAATASMLLCW 306 E G+ FG V + + R V K ++++ G +L A S+ + Sbjct: 246 IELHGVKPQYFGFVFASNAIGLIAASQVNARLVVKRSAERVL--GKVLWVPAGLSLTVSL 303 Query: 307 HLGLLEARSGLLLFAGLYCLTIFGQAVLFPNSMAIAVSGTKNHGAHAMALCGFLQQGLAG 366 + + LLL LT +G PN+ AIA+ A AL G LQ G+ G Sbjct: 304 LVLVGLETLPLLLLGFFGFLTAYG--FTGPNATAIALKHQGRQAGTAAALMGSLQFGM-G 360 Query: 367 IAATVSVLLHHHG 379 + + V++ L H G Sbjct: 361 VLSGVAMSLWHDG 373 >gi|300311754|ref|YP_003775846.1| Bcr/CflA family drug resistance transporter protein [Herbaspirillum seropedicae SmR1] Length = 417 Score = 80.1 bits (196), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 78/297 (26%), Positives = 133/297 (44%), Gaps = 12/297 (4%) Query: 22 MMMLCCLIVFMAQMATTLYLPSLPIVMRELAMSQSLVEMSISGFVVGAALPVLFWGTAAD 81 ++ L C Q+A LY+P+LP + + A S ++ S++G+++ A L +G AD Sbjct: 22 LLFLICAFASAGQLAIDLYVPALPEMAIDYATSAQAIQSSVTGYLIAYAFGQLVFGPLAD 81 Query: 82 RWGRRGPLLASLALFVLCSALLAAVGSAVELLFLRVLQGIAAGGAAIIARIIVRDNWSGD 141 +GR+ L L LF L L A + + RVLQG ++A+ I+ D+++G Sbjct: 82 AYGRKRILALGLGLFSLGCVLSLAAPNLETFVAARVLQGFGIAATNLLAKAIITDSFAGQ 141 Query: 142 ELARRLSVLSIAFITALGAGQFIGGLIGQYSHWQ--IGFVLMAVTGALAAL----LSMTL 195 L + ++IA+ A IG + + W+ + F+L+ G A L ++ Sbjct: 142 ALVHAYTYMAIAWGLAPIIAPVIGAHLQEAFGWRSCLVFLLVYSLGMWAVLWRYRETLAQ 201 Query: 196 PLQGGRQAAARQGGNPYWQILRRPGFVRPACAGGLGFATTITLQEASPFVFQEHFGLAVA 255 P++ AR +L P F A GL ++ + PF+ Q Sbjct: 202 PVRLHPATLARNAR----MVLASPVFQSCFLAQGLCYSILLVFNIVGPFMVQNTLHQPPT 257 Query: 256 SFGNVGLLIGVAYFLGAWTVNRTVSKL--GGKKLMCAGALLIAAATASMLLCWHLGL 310 FG + L IG+ YFLG + T +L ++L ++ AA +LL +GL Sbjct: 258 FFGYLALAIGMMYFLGGISNRITHRRLPTAEQRLRLGARVMTGAAFVMLLLALSVGL 314 >gi|300311040|ref|YP_003775132.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 59.7 bits (143), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 41/143 (28%), Positives = 71/143 (49%), Gaps = 4/143 (2%) Query: 35 MATTLYLPSLPIVMRELAMSQSLVEMSISGFV--VGAALPVLFWGTAADRWGRRGPLLAS 92 M T+ SLP + R+L ++++++ +V +G +P+ W AD+ G R A+ Sbjct: 1 MDATVLATSLPALARDLHQDPITLKLAMTAYVAALGVFIPISGW--MADKLGARKVFSAA 58 Query: 93 LALFVLCSALLAAVGSAVELLFLRVLQGIAAGGAAIIARIIVRDNWSGDELARRLSVLSI 152 + +F+ S L A GS V + R LQGI + R+I+ + EL + +S L++ Sbjct: 59 MIVFMTGSILCAVSGSLVTFVAARFLQGIGGAMMVPVGRVIIARSVDKSELVKAVSYLTL 118 Query: 153 AFITALGAGQFIGGLIGQYSHWQ 175 + G +GG I Y HW+ Sbjct: 119 PALLGPVVGPLLGGFITTYFHWR 141 >gi|300313188|ref|YP_003777280.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 498 Score = 57.4 bits (137), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 49/189 (25%), Positives = 89/189 (47%), Gaps = 4/189 (2%) Query: 21 RMMMLCCLIV---FMAQMATTLYLPSLPIVMRELAMSQSLVEMSISGFVVGAALPVLFWG 77 R+ ML ++ FM + TT+ +LP + + + + + + ++ +++ A+ + G Sbjct: 28 RVRMLPYIVAATFFMEYLDTTIIATALPQMAQSFGVGPNELSLGMTAYMLTLAVFIPISG 87 Query: 78 TAADRWGRRGPLLASLALFVLCSALLAAVGSAVELLFLRVLQGIAAGGAAIIARIIVRDN 137 ADR+G R ++A+F L S L + E RVLQG+ + R+IV + Sbjct: 88 WVADRFGSRTVFGMAIAIFTLASILCGFSQNVWEFTAARVLQGLGGAMMVPVGRMIVVRS 147 Query: 138 WSGDELARRLSVLSIAFITALGAGQFIGGLIGQYSHWQIGFVLMAVTGALAALLSMTLPL 197 D L + ++ ++ I A G IGG I Y+ W F LM V LAA ++ + + Sbjct: 148 TGKDRLMKAIATITWPAIVAPVVGPSIGGFITTYASWHWIF-LMNVPFGLAAFTAVMMVV 206 Query: 198 QGGRQAAAR 206 + + R Sbjct: 207 RNEHGVSKR 215 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.139 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,101,825 Number of extensions: 39817 Number of successful extensions: 303 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 307 Number of HSP's successfully gapped: 12 Length of query: 408 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 321 Effective length of database: 1,207,925 Effective search space: 387743925 Effective search space used: 387743925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)