BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0120.p (662 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspiri... 1338 0.0 gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspiri... 422 e-119 gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum... 417 e-117 gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspiri... 406 e-114 >gi|300311314|ref|YP_003775406.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 662 Score = 1338 bits (3463), Expect = 0.0, Method: Compositional matrix adjust. Identities = 659/662 (99%), Positives = 659/662 (99%) Query: 1 MAGEHALSAGAAPSLHPAYRPDIDGLRAVAVGSVVIFHAFPEWFAGGFIGVDIFFVISGF 60 MAGEHALSAGAAPSLHPAYRPDIDGLRAVAVGSVVIFHAFPEWFAGGFIGVDIFFVISGF Sbjct: 1 MAGEHALSAGAAPSLHPAYRPDIDGLRAVAVGSVVIFHAFPEWFAGGFIGVDIFFVISGF 60 Query: 61 LISLILFKNLEHGRFSIVDFYVRRVRRIFPALMTVMSLCLVTGWFVLFADEYKQLGKHLF 120 LISLILFKNLEHGRFSIVDFYVRRVRRIFPALMTVMSLCLVTGWFVLFADEYKQLGKHLF Sbjct: 61 LISLILFKNLEHGRFSIVDFYVRRVRRIFPALMTVMSLCLVTGWFVLFADEYKQLGKHLF 120 Query: 121 GGSTFISNFLFWRESGYFDNSAETKPLLHLWSLAIEEQFYLFWPLLLAFVWKRRWGFLGI 180 GGSTFISNFLFWRESGYFDNSAETKPLLHLWSLAIEEQFYLFWPLLLAFVWKRRWGFLGI Sbjct: 121 GGSTFISNFLFWRESGYFDNSAETKPLLHLWSLAIEEQFYLFWPLLLAFVWKRRWGFLGI 180 Query: 181 MGVIGLLSFGVNLYFIGHDQSAAFYSPLARFWELMIGGALAYINLHRQDLTRGYKNLQAW 240 MGVIGLLSFGVNLYFIGHDQSAAFYSPLARFWELMIGGALAYINLHRQDLTRGYKNLQAW Sbjct: 181 MGVIGLLSFGVNLYFIGHDQSAAFYSPLARFWELMIGGALAYINLHRQDLTRGYKNLQAW 240 Query: 241 LGAALLVAGFALLDGARAFPGWWALLPSLGAFLLLSAGPGAWFNRRVLASKGMVALGLVS 300 LGAALLVAGFALLDGARAFPGWWALLPSLGAFLLLSAGPGAWFNRRVLASKGMVALGLVS Sbjct: 241 LGAALLVAGFALLDGARAFPGWWALLPSLGAFLLLSAGPGAWFNRRVLASKGMVALGLVS 300 Query: 301 YPLYLWHWPLLAFATIIEGESASAEWRAAAVLGALLLSWLTYRYIERPLRGSTRSRTVLV 360 YPLYLWHWPLLAFATIIEGESA AEWRAAAVLGALLLSWLTYRYIERPLRGSTRSRTVLV Sbjct: 301 YPLYLWHWPLLAFATIIEGESAPAEWRAAAVLGALLLSWLTYRYIERPLRGSTRSRTVLV 360 Query: 361 LLAINVAVGLGGLAIYLKGGFDERAINGKLDYLTQSDVFEGSRNSDGSCGRFNGMEGVDE 420 LLAINVAVGLGGLAIYLKGGFDERAINGKLDYLTQSDVFEGSRNSDGSC RFNGMEGVDE Sbjct: 361 LLAINVAVGLGGLAIYLKGGFDERAINGKLDYLTQSDVFEGSRNSDGSCARFNGMEGVDE 420 Query: 421 EVCLSNSAAPRTLFVGDSHVMALHSAIFSGDVKMPAMMVAGHACRVYPNLPYTPTHLLRW 480 EVCLSNSAAPRTLFVGDSHVMALHSAIFSGDVKMPAMMVAGHACRVYPNLPYTPTHLLRW Sbjct: 421 EVCLSNSAAPRTLFVGDSHVMALHSAIFSGDVKMPAMMVAGHACRVYPNLPYTPTHLLRW 480 Query: 481 ANNCTAIANEAIAVAGKLPSIDTVVISGAYPRGDLSQPSEYQQDGRTLSKREAFLSGYGA 540 ANNCTAIANEAIAVAGKLPSIDTVVISGAYPRGDLSQPSEYQQDGR LSKREAFLSGYGA Sbjct: 481 ANNCTAIANEAIAVAGKLPSIDTVVISGAYPRGDLSQPSEYQQDGRALSKREAFLSGYGA 540 Query: 541 LIEAFRKQGKKVVFMPDVPYLKLDPHACVNRFSDGQARDCAYTEAEHQALRRDYDAAVAA 600 LIEAFRKQGKKVVFMPDVPYLKLDPHACVNRFSDGQARDCAYTEAEHQALRRDYDAAVAA Sbjct: 541 LIEAFRKQGKKVVFMPDVPYLKLDPHACVNRFSDGQARDCAYTEAEHQALRRDYDAAVAA 600 Query: 601 LQRAHPDAVFFDPQGLFCPDGKCQAKLGEAYLYNDNQHISLAASRLLLAEMQRRGLLPAA 660 LQRAHPDAVFFDPQGLFCPDGKCQAKLGEAYLYNDNQHISLAASRLLLAEMQRRGLLPAA Sbjct: 601 LQRAHPDAVFFDPQGLFCPDGKCQAKLGEAYLYNDNQHISLAASRLLLAEMQRRGLLPAA 660 Query: 661 QP 662 QP Sbjct: 661 QP 662 >gi|300311522|ref|YP_003775614.1| O-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 680 Score = 422 bits (1084), Expect = e-119, Method: Compositional matrix adjust. Identities = 260/681 (38%), Positives = 355/681 (52%), Gaps = 80/681 (11%) Query: 13 PSL-HPAYRPDIDGLRAVAVGSVVIFHAFPEWFAGGFIGVDIFFVISGFLISLILFKNLE 71 P+L H YR DIDGLRA+AV +VV FH FP + GGFIGVD+FFVISG+LIS I+F+NL+ Sbjct: 7 PALSHSKYRSDIDGLRAIAVLAVVSFHGFPNFVRGGFIGVDVFFVISGYLISTIIFENLD 66 Query: 72 HGRFSIVDFYVRRVRRIFPALMTVMSLCLVTGWFVLFADEYKQLGKHLFGGSTFISNFLF 131 G F+ +FY RRVRRIFPAL+ V+S C V GWFVL DEYKQLGKH+ GG+ F+SNFL Sbjct: 67 RGTFTFTEFYARRVRRIFPALLLVLSTCFVVGWFVLLTDEYKQLGKHIAGGAGFVSNFLL 126 Query: 132 WRESGYFDNSAETKPLLHLWSLAIEEQFYLFWPLLLAFVWKRRWGFLGIMGVIGLLSFGV 191 ESGYFDNSAETKPLLHLWSL IEEQFY+ WP+LL F W+R++ L I ++ L SF Sbjct: 127 RDESGYFDNSAETKPLLHLWSLGIEEQFYILWPMLLFFSWRRKFNLLTITIIVALASFAA 186 Query: 192 NLYFIGHDQSAAFYSPLARFWELMIGGALAYINLHRQDLTRGYK-NLQAWL--------- 241 N+ I D +AAFY P +RFWEL+ G LA+ L++Q T + L W+ Sbjct: 187 NIKGINKDATAAFYLPQSRFWELLSGTLLAWFTLYKQHTTIELRPRLGTWILQLIYSEKR 246 Query: 242 --------------GAALLVAGFALLDGARAFPGWWALLPSLGAFLLLSAGPGAWFNRRV 287 G +++ GF ++ AFPG WAL+P LG L+++AGP AW NR + Sbjct: 247 KSDGKFLIDVSSITGLCIVLYGFWRINKELAFPGVWALVPILGTILIIAAGPAAWVNRTL 306 Query: 288 LASKGMVALGLVSYPLYLWHWPLLAFATIIEGESASAEWRAAAVLGALLLSWLTYRYIER 347 L++K +V GL+S+PLYLWHWP+L+FA I+E E S R A+ A++L+WLTY +ER Sbjct: 307 LSNKILVWFGLISFPLYLWHWPVLSFARIVENELPSYPLRVGAIALAIILAWLTYEIVER 366 Query: 348 PLRGSTRSR---TVLVLLAINVAVGLGGLAIYLKGGFDERAINGKLDYLTQSDVFEGSRN 404 P+R + TV L+ + VG G + G++ R D+ SR Sbjct: 367 PIRTGNNANTKITVTALIGLMALVGAVGYRTFAANGWESR---------MSPDITRISRA 417 Query: 405 SD--GSCGRFNGMEGVDEEVCLSNSAAPR-TLFVGDSHVMALHSAIFSGDVKMP-----A 456 + G+ + NS TLF+GDS++ + P Sbjct: 418 AGEWQYPGKLTAFNFENRTFYRQNSNMDEITLFIGDSNIEQYYVRTDELISTKPNNSNSV 477 Query: 457 MMVAGHACRVYPNLPYTPTHLLRWANNCTAIANEAIAVAGKLPSIDTVVISGAYPRGDLS 516 + G C P PY H+ +C + A+ A S+ +VVI + Sbjct: 478 VFATGGGCLAIPGSPYDEAHM-----SCANLMETALNYAKTNASVASVVIG--------A 524 Query: 517 QPSEYQQDGRTL--------SKREAFLSGYGALIEAFRKQGKKVVFMPDVPY-LKLDPHA 567 Q + Y DG L A L+ I + GKKV F+ ++P +LDP Sbjct: 525 QWNSYLSDGYGLRGSFGTAGKDYSAALNRLARYIRELKYLGKKVYFILNIPIGDELDPKF 584 Query: 568 CVNRFSDGQARDCAYTEAEHQALRRDYDAAVAALQR--------AHPDAVFFDPQGLFCP 619 + R + + E R+D D +Q+ A D V +P C Sbjct: 585 MITR--NLKHFPHVINLKERGLNRKDIDERYGKIQKDLEQIARAAGADVV--NPMDFLCN 640 Query: 620 DGKCQAKLGEAY-LYNDNQHI 639 KC + E +Y D H+ Sbjct: 641 ATKCSSLDDEGEPIYKDRSHL 661 >gi|300310314|ref|YP_003774406.1| acyltransferase [Herbaspirillum seropedicae SmR1] Length = 656 Score = 417 bits (1073), Expect = e-117, Method: Compositional matrix adjust. Identities = 282/671 (42%), Positives = 369/671 (54%), Gaps = 58/671 (8%) Query: 1 MAGEHALSAGAAP--SLHPAYRPDIDGLRAVAVGSVVIFHAFPEWFAGGFIGVDIFFVIS 58 +A H AGAA + HP YRPDIDGLRA+AV SVV+FHAFPEW GGF+GVDIFFVIS Sbjct: 2 LASNHKTPAGAASPATAHPGYRPDIDGLRAIAVLSVVVFHAFPEWLPGGFVGVDIFFVIS 61 Query: 59 GFLISLILFKNLEHGRFSIVDFYVRRVRRIFPALMTVMSLCLVTGWFVLFADEYKQLGKH 118 GFLIS ILF L+ RFSIVDFY+RR+RRIFPAL+ VM CL+ GWF+L +EY+QLGKH Sbjct: 62 GFLISGILFDALQRDRFSIVDFYIRRIRRIFPALLLVMLACLLAGWFILLPEEYRQLGKH 121 Query: 119 LFGGSTFISNFLFWRESGYFDNSAETKPLLHLWSLAIEEQFYLFWPLLLAFVWKRRWGFL 178 L GG+ F+SN + W E+GYFD SAETKPLLHLWSL +EEQFYLFWPL+L W+RR G L Sbjct: 122 LLGGAGFVSNIVLWNEAGYFDTSAETKPLLHLWSLGVEEQFYLFWPLMLWAAWRRRTGML 181 Query: 179 GIMGVIGLLSFGVNLYFIGHDQSAAFYSPLARFWELMIGGALAYINLHRQDLTRGYKNLQ 238 ++ + LSFG N+ ++ D AAFY P++RFWEL G LA+ + T + Sbjct: 182 AVLLGVMALSFGWNIVWLHRDAVAAFYLPMSRFWELGAGALLAWRHRQGAVPTSVQAHRL 241 Query: 239 AWLGAALLVAGFALLDGARAFPGWWALLPSLGAFLLLSAGPGAWFNRRVLASKGMVALGL 298 + G L+V A + AFPGWWALLP GA LLL+AGP A NR +L+ + MV +GL Sbjct: 242 SVAGLLLIVVAIATITKDDAFPGWWALLPVTGAVLLLAAGPQARINRSLLSHRTMVWVGL 301 Query: 299 VSYPLYLWHWPLLAFATIIEGESASAEWRAAAVLGALLLSWLTYRYIERPLR-GSTRSRT 357 +SYPLYLWHWPLLAFA ++ G + S + R AAV ALLL+WLTYR +E P+R S R+ Sbjct: 302 ISYPLYLWHWPLLAFARLVVGATPSVQLRLAAVALALLLAWLTYRLVELPVRLRSAGHRS 361 Query: 358 VLVLLAINVAVGLGGLAIYLKGGFDERAINGKL----DYLTQSDVFEGSRNSDGSCGRFN 413 + L + + G + + G D R + D + ++ V SC Sbjct: 362 AVTLALLMALMAALGGYVVKRNGMDFRQLGAVAMLFNDQVKETAVLNHFELPHPSCAALM 421 Query: 414 GMEGVDEEVCLSNSA-APRTLFVGDSHVMALHSAIFSGDVKMPAMMVAGHACRVYPNLPY 472 G E + SA P+ L +GDS+ A A M+A R+Y P Sbjct: 422 GQEHSRDWCAAPVSAEPPQVLLIGDSYSGAY------------APMLA----RLYQASPQ 465 Query: 473 TPTHLLRWANN------------CTAIANEAIAVAGKLPSIDTVVISGAYPRGDLSQPSE 520 ++A C I+ A A + PSI TVV++ +P Sbjct: 466 PQLVFQQFARGGCASLLDGGGGYCGEISERVAAYARQTPSIKTVVLAANWP--------- 516 Query: 521 YQQDGRTLSKREAFLSGYGALIEAFRKQGKKVVFMPDVPYLKLDPHACV---NRFSDGQA 577 G + + L I +R+ GK+VV + P +P +CV R SD A Sbjct: 517 ----GHIAANPKGVLKALEHTILYYRQLGKRVVVLLSPPN-GANPKSCVLRGIRLSD--A 569 Query: 578 RDCAYTEAEHQALRRDY-DAAVAALQRAHPDAVFFDPQGLFCPDGKCQAKLGEAYLYNDN 636 C A+ + + Y D + LQR D FDP C + C+ G LY D Sbjct: 570 DFCNLPRAKAEQMDDHYRDLMLPLLQRL--DVPVFDPYPTLCDEHGCKVIDGPRILYFDP 627 Query: 637 QHISLAASRLL 647 H+S ++ L Sbjct: 628 GHLSAYGAQYL 638 >gi|300310014|ref|YP_003774106.1| o-antigen acetylase [Herbaspirillum seropedicae SmR1] Length = 681 Score = 406 bits (1043), Expect = e-114, Method: Compositional matrix adjust. Identities = 258/652 (39%), Positives = 355/652 (54%), Gaps = 53/652 (8%) Query: 18 AYRPDIDGLRAVAVGSVVIFHAFPEWFAGGFIGVDIFFVISGFLISLILFKNLEHGRFSI 77 +YRPDIDGLRA+A+ SVV+FHAFP+ GGFIGVDIFFVISGFLIS I+F+ L+ GRFS Sbjct: 31 SYRPDIDGLRAIAIVSVVVFHAFPDAMPGGFIGVDIFFVISGFLISSIIFRQLDQGRFSF 90 Query: 78 VDFYVRRVRRIFPALMTVMSLCLVTGWFVLFADEYKQLGKHLFGGSTFISNFLFWRESGY 137 DFYV R+RRIFPAL+ V++ C V GWF L +EY+QLGKH+ G+ FI N + W+ESGY Sbjct: 91 TDFYVHRIRRIFPALILVLASCFVLGWFSLLPEEYRQLGKHMAAGAGFIQNLVLWKESGY 150 Query: 138 FDNSAETKPLLHLWSLAIEEQFYLFWPLLLAFVWKRRWGFLGIMGVIGLLSFGVNLYFIG 197 FD ++ KPLLHLWSL++EEQFYL +PLL+ +W+ R + ++ L SF +NL+++ Sbjct: 151 FDVESDMKPLLHLWSLSVEEQFYLVYPLLVCGLWRARMPLRNSLVLLMLASFALNLWWVA 210 Query: 198 HDQSAAFYSPLARFWELMIGGALAYINLHRQDL---TRGYK-------NLQAWLGAALLV 247 D FY P R WEL+ GAL + R+ + T ++ +L++ LG ALL+ Sbjct: 211 QDPVKTFYLPFTRVWELL-AGALCAFGVMRKGVAFSTSSFRLPHLPSIHLRSVLGLALLL 269 Query: 248 AGFALLDGARAFPGWWALLPSLGAFLLLSAGPGAWFNRRVLASKGMVALGLVSYPLYLWH 307 G L+D R FPG WALLP GA LL+S+GP AW NR +L+ +V +GLVSY LYLWH Sbjct: 270 CGLVLIDRHRYFPGAWALLPVTGAVLLVSSGPQAWVNRHLLSRPLLVWIGLVSYLLYLWH 329 Query: 308 WPLLAFATIIEGESASAEWRAAAVLGALLLSWLTYRYIERPLRGSTRSRTVLVLLAINVA 367 WP+L+F I+ A AAV ++LL+WLTYR +ERP+R +S V LLA+ ++ Sbjct: 330 WPILSFMHILTPTRPGAPLPLAAVALSVLLAWLTYRLLERPVRFGAKSWMVPALLALLLS 389 Query: 368 V-GLGGLAIYLKGGFDERAINGKLDYLTQSDVFEGSRNSDGSCGRFNGMEGVDEEVC-LS 425 V G + + G R + + +F G+ +D +C G E C S Sbjct: 390 VTATAGYNDFQRNGLSFRLAT----HYAEQPLFVGT--TDATC---TADGGAGLEFCRQS 440 Query: 426 NSAAPRTLFVGDSHVMALHSAIFS-----GDVKMPAMMVAGHACRVYPNLPYTPTHLLRW 480 AP + +GDSH L+ + S GD+ M A AC + + Sbjct: 441 RPGAPTIVVIGDSHAWHLYDGLASYAAQNGDI---IGMRAVGACLGFSGVSADTKR---- 493 Query: 481 ANNCTAIANEAIAVAGKLPSIDTVVISGAYPRGDLSQPSEYQQDGRTLSKREAFLSGYGA 540 ++C A A ++IA A + S+ TVV+S D P E F Sbjct: 494 -HDC-APARDSIASAARQASVRTVVLSARLDIYDRELPG------------EVFEGALRD 539 Query: 541 LIEAFRKQGKKVVFMPDVPYLKLDPHACVN-----RFSDGQARDCAYTEAEHQALRRDYD 595 ++ GKKV+F+ D+P L P C++ R +CA +A + Y Sbjct: 540 MLATLTAAGKKVIFVMDIPILDFHPKYCLDISMPLRPKTTSLPNCAIQQAVFAQHTQHYR 599 Query: 596 AAVAALQRAHPDAVFFDPQGLFCPDGKCQAKLGEAYLYNDNQHISLAASRLL 647 V AL R P FD C +C A LY D H+S S L+ Sbjct: 600 ETVNALLRQFPQVQAFDAAAPLCDHQQCWAMKDGQLLYRDYDHLSTIGSTLV 651 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.141 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,086,456 Number of extensions: 89158 Number of successful extensions: 382 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 367 Number of HSP's successfully gapped: 6 Length of query: 662 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 571 Effective length of database: 1,188,985 Effective search space: 678910435 Effective search space used: 678910435 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 134 (56.2 bits)