BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0094.p (526 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313003|ref|YP_003777095.1| histidine ammonia-lyase [Herbas... 1067 0.0 gi|300313821|ref|YP_003777913.1| histidine ammonia-lyase [Herbas... 180 2e-46 >gi|300313003|ref|YP_003777095.1| histidine ammonia-lyase [Herbaspirillum seropedicae SmR1] Length = 526 Score = 1067 bits (2760), Expect = 0.0, Method: Compositional matrix adjust. Identities = 522/526 (99%), Positives = 525/526 (99%) Query: 1 MPHTELTSSASSAPIRFGAQPLAIEDVVALARRERSAQLHDAPDFRARIARGADFLDRLL 60 MPHT+LTSSASS PIRFGAQPLAIEDVVALARRERSAQLHDAPDFRARIARGADFLDRLL Sbjct: 1 MPHTDLTSSASSTPIRFGAQPLAIEDVVALARRERSAQLHDAPDFRARIARGADFLDRLL 60 Query: 61 REEGTIYGVTTGYGDSCTVTVPPELVPELPHHLYTYHGCGLGALFTPEQARAILAARLNS 120 RE+GTIYGVTTGYGDSCTVTVPPELVPELPHHLYTYHGCGLGALFTPEQARAILAARLNS Sbjct: 61 REDGTIYGVTTGYGDSCTVTVPPELVPELPHHLYTYHGCGLGALFTPEQARAILAARLNS 120 Query: 121 LCQGFSGVSVALLEQITGLLQHDLLPQIPCEGSVGASGDLTPLSYLAAVLCGERDVWREG 180 LCQGFSGVSVALLEQITGLLQHDLLPQIPCEGSVGASGDLTPLSYLAAVLCGERDVWREG Sbjct: 121 LCQGFSGVSVALLEQITGLLQHDLLPQIPCEGSVGASGDLTPLSYLAAVLCGERDVWREG 180 Query: 181 ATVPAAQALAAAGMTPLRLRPKEGLAIMNGTAVMTALACLAFDRARYLCQLATRITAMSS 240 ATVPAAQALAAAGMTPLRLRPKEGLAIMNGTAVMTALACLAFDRARYLCQLATRITAMSS Sbjct: 181 ATVPAAQALAAAGMTPLRLRPKEGLAIMNGTAVMTALACLAFDRARYLCQLATRITAMSS 240 Query: 241 FTLDGNAHHFDATLFSAKPHPGQQQVAAWLQRDLPCGQAHRNEKRLQDRYSVRCAPHVIG 300 FTLDGNAHHFDATLFSAKPHPGQQQVAAWLQRDLPCGQAHRNEKRLQDRYSVRCAPHVIG Sbjct: 241 FTLDGNAHHFDATLFSAKPHPGQQQVAAWLQRDLPCGQAHRNEKRLQDRYSVRCAPHVIG 300 Query: 301 VLNDALPSLRQFIENELNSANDNPLIDPDGERVLHGGHFYGGHIAFAMDSMKTAVANIAD 360 VLNDALPSLRQFIENELNSANDNPLIDPDGERVLHGGHFYGGHIAFAMDSMKTAVAN+AD Sbjct: 301 VLNDALPSLRQFIENELNSANDNPLIDPDGERVLHGGHFYGGHIAFAMDSMKTAVANVAD 360 Query: 361 LLDRQMALMVDQRYNNGLPANLSGAQGARAPINHGLKALQISASAWTAEALKLTMPASVF 420 LLDRQMALMVDQRYNNGLPANLSGAQGARAPINHGLKALQISASAWTAEALKLTMPASVF Sbjct: 361 LLDRQMALMVDQRYNNGLPANLSGAQGARAPINHGLKALQISASAWTAEALKLTMPASVF 420 Query: 421 SRSTECHNQDKVSMGTIAARDCLRVLELTEQVAAALLITVRQGVWLRSQRQQVGDTPLQD 480 SRSTECHNQDKVSMGTIAARDCLRVLELTEQVAAALLITVRQGVWLRSQRQQVGDTPLQD Sbjct: 421 SRSTECHNQDKVSMGTIAARDCLRVLELTEQVAAALLITVRQGVWLRSQRQQVGDTPLQD 480 Query: 481 LPAGRLAAMQAALSEDIAVVAEDRMLEPDLRLLLQRIRAQAWSLYD 526 LPAGRLAAMQAALSEDIAVVAEDRMLEPDLRLLLQRIRAQAWSLYD Sbjct: 481 LPAGRLAAMQAALSEDIAVVAEDRMLEPDLRLLLQRIRAQAWSLYD 526 >gi|300313821|ref|YP_003777913.1| histidine ammonia-lyase [Herbaspirillum seropedicae SmR1] Length = 542 Score = 180 bits (457), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 138/450 (30%), Positives = 227/450 (50%), Gaps = 33/450 (7%) Query: 46 RARIARGADFLDR--LLREEGTIYGVTTGYGDSCTVTVPPELVPELPHHLYTYHGCGLGA 103 RA + + D+++ + E +Y TG G + E + L H G+G Sbjct: 34 RAALKQSRDYIESTWMHDEAPMMYSFNTGVGLLKDTRIKVEHIELFQTQLIRAHSAGIGE 93 Query: 104 LFTPEQARAILAARLNSLCQGFSGVSVALLEQITGLLQHDLLPQIPCEGSVGASGDLTPL 163 F+ E +RA + R N+ +S V +++++ + + P +P +GSVGASGDL PL Sbjct: 94 AFSEEVSRATMLLRANAFASNYSAPRVEVVDRLLAFINAGIHPIMPQKGSVGASGDLAPL 153 Query: 164 SYLAAVLCG--ERDVWREGATVPAAQALAAAGMTPLR--LRPKEGLAIMNGTAVMTALAC 219 +YLAA + G E +V +G + A +A+ AG+ P++ L+ K+ A++NG A+A Sbjct: 154 AYLAAAIAGFDEAEVMYQGQRMRATEAIEQAGIGPVKFDLKAKDASALINGCTASLAVAI 213 Query: 220 LAFDRARYLCQLATRITAMSSFTLDGNAHHFDATLFSAKPHPGQQQVA----AWLQRDLP 275 LA AR+L A ++ + FD + A+PH GQ + A LQ Sbjct: 214 LAAHDARHLLTDACLSLGLTLEAMRAEMSAFDPRIQQARPHAGQIKTAEVIRTLLQGSTR 273 Query: 276 CGQAHRNEK--------------RLQDRYSVRCAPHVIGVLNDALPSLRQFIENELNSAN 321 A R + R+QD YS+RCAP V G + DAL + IE E NSA Sbjct: 274 TTHAARAVQLPDELRRTDIAYTARIQDVYSLRCAPQVYGPVFDALDYIDTIIEKETNSAT 333 Query: 322 DNPLI----DPDGERVLHGGHFYGGHIAFAMDSMKTAVANIADLLDRQMALMVDQRYNNG 377 DNPLI + +G ++ GG+F+G ++A AMD + A+ ++ + +R++A ++D + G Sbjct: 334 DNPLIFHKEEGEGFEIISGGNFHGQYLAQAMDLLAMAITDLGSICERRIARLIDPTLSWG 393 Query: 378 LPANL-SGAQGARAPINHGLKALQISASAWTAEALKLTMPASVFSRSTECHNQDKVSMGT 436 LP NL SG +G +N G +Q S S+ E L MP SV S ++ +++D +S T Sbjct: 394 LPRNLMSGVRG----VNTGYPVVQCSMSSLVMENRTLCMPGSVDSIPSKGNSEDHISNST 449 Query: 437 IAARDCLRVLELTEQVAAALLITVRQGVWL 466 AR + V++ T+ + ++ Q + + Sbjct: 450 WCARKAMTVVQNTQYIVGVEMLLAAQALTM 479 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,569,783 Number of extensions: 63582 Number of successful extensions: 205 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 202 Number of HSP's successfully gapped: 2 Length of query: 526 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 436 Effective length of database: 1,193,720 Effective search space: 520461920 Effective search space used: 520461920 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)