BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0087.p (245 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 495 e-141 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 176 2e-45 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 166 1e-42 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 138 4e-34 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 112 2e-26 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 495 bits (1275), Expect = e-141, Method: Compositional matrix adjust. Identities = 245/245 (100%), Positives = 245/245 (100%) Query: 1 MTTTGAHSIFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRV 60 MTTTGAHSIFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRV Sbjct: 1 MTTTGAHSIFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRV 60 Query: 61 LQFDVANREQAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFY 120 LQFDVANREQAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFY Sbjct: 61 LQFDVANREQAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFY 120 Query: 121 NVLNPLVMPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDI 180 NVLNPLVMPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDI Sbjct: 121 NVLNPLVMPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDI 180 Query: 181 TVNCVAPGLIDTDMTEDAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISV 240 TVNCVAPGLIDTDMTEDAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISV Sbjct: 181 TVNCVAPGLIDTDMTEDAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISV 240 Query: 241 NGGLA 245 NGGLA Sbjct: 241 NGGLA 245 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 176 bits (445), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 111/240 (46%), Positives = 148/240 (61%), Gaps = 8/240 (3%) Query: 10 FVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQV-RVLQFDVANR 68 VTG+SRGIGRAIA LAR+G ++ + AA + +AALG + R +V N Sbjct: 12 LVTGASRGIGRAIATELARQGAKVIGTATSESGAA-AITEYLAALGPEAGRGAVLNV-ND 69 Query: 69 EQAAQVLLADVEQ-HGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNVLNPLV 127 +A+ L+ V++ G +V NAGIT+D M E+WDSV+ TNL + ++ Sbjct: 70 AEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRAVL 129 Query: 128 MPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVNCVAP 187 M++ R GRI+ + SV G GN GQ+NY+AAKAGV G ++ALA E+ R+ITVNCVAP Sbjct: 130 RGMMKART-GRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNCVAP 188 Query: 188 GLIDTDMT---EDAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISVNGGL 244 G IDTDMT + IL+ IP R G EE+A AVAFL SP AAYIT ++VNGG+ Sbjct: 189 GFIDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNVNGGM 248 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 166 bits (420), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 94/238 (39%), Positives = 134/238 (56%), Gaps = 4/238 (1%) Query: 10 FVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRVLQFDVANRE 69 +VTG GIG AI RL ++GY +V C D+ A + G + + +V+N E Sbjct: 7 YVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQGFDIHASEGNVSNWE 66 Query: 70 QAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNVLNPLVMP 129 + G +V NAGITRD F M +DWD+V+ TNL+ +NV ++ Sbjct: 67 STKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVTKQVIEG 126 Query: 130 MVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVNCVAPGL 189 M R GRI+ ++SV+G G GQ NYS AKAG+ G T ALA E+A + +TVN V+PG Sbjct: 127 MCER-GFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGY 185 Query: 190 IDTDMT---EDAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVISVNGGL 244 I TDM +++I+ IP +R G+PEE+A+ VA++ S + Y T S+NGGL Sbjct: 186 IGTDMVRAIRPEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLNGGL 243 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 138 bits (347), Expect = 4e-34, Method: Compositional matrix adjust. Identities = 89/245 (36%), Positives = 136/245 (55%), Gaps = 6/245 (2%) Query: 4 TGAHSIFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRVLQF 63 +GA VTG + GIG +I+ +LA +G+ VL DAA + A + A G + LQ Sbjct: 6 SGARVALVTGGAMGIGASISQQLAAKGH-TVLVADINLDAASKTADALVAAGHRAAPLQM 64 Query: 64 DVANREQAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDGFYNVL 123 D+ + E A + +G +V NAG+ R +F P E+W + N+ G Sbjct: 65 DLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAG 124 Query: 124 NPLVMPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRDITVN 183 MV+++ GRIV ++S+SG+ G+ Y +KA VIG T+ +A+ELA+ ITVN Sbjct: 125 QHAARLMVKQQW-GRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVN 183 Query: 184 CVAPGLIDTDMTE----DAPIDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVIS 239 VAPG +DT MT+ + + +L+P RR G +E+A+AV +L + ++AYIT VI Sbjct: 184 SVAPGPVDTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAEESAYITGHVIP 243 Query: 240 VNGGL 244 V+GG Sbjct: 244 VDGGF 248 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 112 bits (281), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 85/250 (34%), Positives = 130/250 (52%), Gaps = 28/250 (11%) Query: 9 IFVTGSSRGIGRAIALRLAREGYDIVLHCHARRDAADQVAAEIAALGRQVRVL------- 61 + VTG++ GIG A A R + EG ++L A RDAA ALG+ L Sbjct: 17 VIVTGAASGIGEATARRFSDEGARVLL---ADRDAA--------ALGKVFDSLPPERTAA 65 Query: 62 -QFDVANREQAAQVLLADVEQHGCYYGVVCNAGITRDNAFPAMPGEDWDSVLRTNLDG-F 119 + DV++ EQ Q++ +E+ G +V +AG+ + + EDW V TN++G F Sbjct: 66 RETDVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVF 125 Query: 120 YNVLNPLVMPMVRRRAAGRIVTLASVSGLIGNRGQVNYSAAKAGVIGATKALALELAKRD 179 Y L P + + G IV +ASVSGL + Y+A+K V T+A+AL+ ++ Sbjct: 126 YGAREAL--PHLEK-TRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKG 182 Query: 180 ITVNCVAPGLIDT----DMTEDAP-IDEILKLIPARRAGKPEEVAAAVAFLMSPDAAYIT 234 + +N V P L T DM +D P +D+ + I R P E+AA + FL SPDA+++ Sbjct: 183 VRINAVCPSLTHTAMTADMADDPPLLDKFAERIALGRGADPLEIAAVITFLASPDASFVN 242 Query: 235 RQVISVNGGL 244 + V+GGL Sbjct: 243 GVNLPVDGGL 252 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 733,051 Number of extensions: 29134 Number of successful extensions: 263 Number of sequences better than 1.0e-08: 46 Number of HSP's gapped: 189 Number of HSP's successfully gapped: 46 Length of query: 245 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 162 Effective length of database: 1,226,865 Effective search space: 198752130 Effective search space used: 198752130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)