BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0080.p (296 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxy... 620 e-179 gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herba... 136 3e-33 gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herba... 125 4e-30 gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase... 68 9e-13 >gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 296 Score = 620 bits (1599), Expect = e-179, Method: Compositional matrix adjust. Identities = 296/296 (100%), Positives = 296/296 (100%) Query: 1 MHPAKEETMLNLNEDTITQAVLSRHAWLTDQRLREIMTSLVQHLHAFAREVKLTEKEWFA 60 MHPAKEETMLNLNEDTITQAVLSRHAWLTDQRLREIMTSLVQHLHAFAREVKLTEKEWFA Sbjct: 1 MHPAKEETMLNLNEDTITQAVLSRHAWLTDQRLREIMTSLVQHLHAFAREVKLTEKEWFA 60 Query: 61 GIDFLTETGQICSDQRQEFILLSDTLGLSTLVMAQQNHKPAGCTEATVFGPFHVEDAPQY 120 GIDFLTETGQICSDQRQEFILLSDTLGLSTLVMAQQNHKPAGCTEATVFGPFHVEDAPQY Sbjct: 61 GIDFLTETGQICSDQRQEFILLSDTLGLSTLVMAQQNHKPAGCTEATVFGPFHVEDAPQY 120 Query: 121 PLGADIANGAKGEPCFVSGSVRGRNGRPLAHARMEVWQADENGFYDVQYADLDQYRARGT 180 PLGADIANGAKGEPCFVSGSVRGRNGRPLAHARMEVWQADENGFYDVQYADLDQYRARGT Sbjct: 121 PLGADIANGAKGEPCFVSGSVRGRNGRPLAHARMEVWQADENGFYDVQYADLDQYRARGT 180 Query: 181 LFTDAEGRYHFRSILANPYPIPHDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHV 240 LFTDAEGRYHFRSILANPYPIPHDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHV Sbjct: 181 LFTDAEGRYHFRSILANPYPIPHDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHV 240 Query: 241 FRRDDVYLDSDAVFGVRSTLIADWPCHPAGTAPDGTQCEQPFYTLDYDFVLNPLRK 296 FRRDDVYLDSDAVFGVRSTLIADWPCHPAGTAPDGTQCEQPFYTLDYDFVLNPLRK Sbjct: 241 FRRDDVYLDSDAVFGVRSTLIADWPCHPAGTAPDGTQCEQPFYTLDYDFVLNPLRK 296 >gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 303 Score = 136 bits (342), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 87/285 (30%), Positives = 139/285 (48%), Gaps = 11/285 (3%) Query: 15 DTITQAVLSRHAWLTDQRLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTETGQICSD 74 D + Q + S +QR++ ++ LV+ + E+ + +E++A +D+LT G+ Sbjct: 7 DAVLQKIESTELAEGNQRVKTVVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSAGK---- 62 Query: 75 QRQEFILLSDTLGLSTLVMAQQNHKPA-----GCTEATVFGPFHVEDAPQYPLGADIANG 129 EF L++ LG + + + A G T T+ GP +V AP+ A + Sbjct: 63 -SGEFGLIAAGLGFEHFLDLRMDEAEARRGVQGGTPRTIEGPLYVSGAPEQKGFARMDKD 121 Query: 130 AK-GEPCFVSGSVRGRNGRPLAHARMEVWQADENGFYDVQYADLDQYRARGTLFTDAEGR 188 + G+ F+ G V +G+PLA+A +EVW A+ G Y D + R ++ TD +GR Sbjct: 122 PQPGDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQSPFNLRCSIRTDEQGR 181 Query: 189 YHFRSILANPYPIPHDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHVFRRDDVYL 248 Y FRS + Y +P G R+L KLGRH RPAH+HF + GY L T + D YL Sbjct: 182 YRFRSRVPVGYSVPPGGATDRLLTKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAGDPYL 241 Query: 249 DSDAVFGVRSTLIADWPCHPAGTAPDGTQCEQPFYTLDYDFVLNP 293 D F R L+ + + + PFY ++++F L P Sbjct: 242 WDDFAFATREGLVPELKHVTDPAEIRKHEVDAPFYAIEFNFNLLP 286 >gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 311 Score = 125 bits (314), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 82/272 (30%), Positives = 130/272 (47%), Gaps = 14/272 (5%) Query: 32 RLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTETGQICSDQRQEFILLSDTLGLSTL 91 R ++I+ ++ L ++++T E++AG+ ++ G E LLS LGL Sbjct: 29 RTKQIVHRILSDLFKTIEDLEITSDEYWAGVAYINRLGTA-----HEAGLLSPGLGLDRF 83 Query: 92 VMAQQNHKPA-----GCTEATVFGPFHVEDAPQYPLGADIANGA--KGEPCFVSGSVRGR 144 + + + + A G T T+ GP +V AP A + +G+ G+ + G+V G Sbjct: 84 LDMRMDAEDAALGIAGGTPRTIEGPLYVAGAPVEQGFARLDDGSDKSGQTIIMHGTVYGA 143 Query: 145 NGRPLAHARMEVWQADENGFYD--VQYADLDQYRARGTLFTDAEGRYHFRSILANPYPIP 202 +G+P+ A++EVW A+ GFY + + R T+ TDA+GRY FRSI+ Y P Sbjct: 144 DGKPMPGAQVEVWHANTKGFYSHFDPTGEQKPFNMRRTIITDAQGRYKFRSIMPTAYGCP 203 Query: 203 HDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHVFRRDDVYLDSDAVFGVRSTLIA 262 GP +L LGRH RPAH+HF + AEG+ L T + D + D + R L+ Sbjct: 204 PAGPTQGLLNALGRHGNRPAHIHFFVTAEGHRKLTTQINIEGDPLIYDDFAYATREGLVP 263 Query: 263 DWPCHPAGTAPDGTQCEQPFYTLDYDFVLNPL 294 + E PF + +D L L Sbjct: 264 PLVERTDAASIKAEGLEGPFAEIVFDIKLTSL 295 >gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase beta chain protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 68.2 bits (165), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 48/146 (32%), Positives = 67/146 (45%), Gaps = 25/146 (17%) Query: 121 PLGADIANGAKGEP----CFVSGSVRGRNGRPLAHARMEVWQADENGFY----DVQYADL 172 P D+ KGEP V+G V +G+P+ ++ +EVWQ + G Y D A L Sbjct: 53 PHDMDLTKQGKGEPLGEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRYFHKKDQHDAPL 112 Query: 173 D-QYRARGTLFTDAEGRYHFRSILANPYPIPHDGPVGRMLEKLGRH--PWRPAHLHFRIQ 229 D + G + TD GRY F SI PYP G H WRPAH+HF + Sbjct: 113 DPNFTGFGKMLTDDNGRYRFVSIKPGPYP-------------WGNHHKAWRPAHIHFSLF 159 Query: 230 AEGY-ETLITHVFRRDDVYLDSDAVF 254 Y + L+T ++ +D D +F Sbjct: 160 GNVYAQRLVTQMYFPNDPLFAYDPIF 185 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,012,578 Number of extensions: 45106 Number of successful extensions: 103 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 98 Number of HSP's successfully gapped: 4 Length of query: 296 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 211 Effective length of database: 1,217,395 Effective search space: 256870345 Effective search space used: 256870345 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)