BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0074.p (556 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313022|ref|YP_003777114.1| two component sensor histidine ... 1125 0.0 gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729... 173 3e-44 gi|300311948|ref|YP_003776040.1| sensor histidine kinase/respons... 152 7e-38 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 145 8e-36 gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kin... 131 1e-31 >gi|300313022|ref|YP_003777114.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 1125 bits (2909), Expect = 0.0, Method: Compositional matrix adjust. Identities = 553/556 (99%), Positives = 554/556 (99%) Query: 1 MDDRKQQSLPLRFARIDLRVALSLFSLLLICGLWLGAFKELQNSRNNYLDDARRDAQSLS 60 MDDRKQQS PLRFARIDLRVALSLFSLLLICGLWLGAFKELQNSRNNYLDDARRDAQSLS Sbjct: 1 MDDRKQQSFPLRFARIDLRVALSLFSLLLICGLWLGAFKELQNSRNNYLDDARRDAQSLS 60 Query: 61 RLFLEHAYRTIEAADQAALYLRYRYAERGNALNLATEIDNGLVARNVYNLFSIVDAQGDV 120 RLFLEHAYRTIEAADQAALYLRYRYAERGNALNLATEIDNGLVARNVYNLFSIVDAQGDV Sbjct: 61 RLFLEHAYRTIEAADQAALYLRYRYAERGNALNLATEIDNGLVARNVYNLFSIVDAQGDV 120 Query: 121 VLSSKPFTPVNLADREHVQVHMQGGQDQLFISKPVLGRVSKKWSLQITRRINRPDGGFGG 180 VLSSKPFTPVNLADREHVQVHMQGGQDQLFISKPVLGRVSKKWSLQITRRINRPDGGFGG Sbjct: 121 VLSSKPFTPVNLADREHVQVHMQGGQDQLFISKPVLGRVSKKWSLQITRRINRPDGGFGG 180 Query: 181 VVVVSMDPQYFTRLYHQIDVGHHGVITLVGADGVARVRRTGDMDAMGEQVAGGKVYAAML 240 VVVVSMDPQYFTRLYHQIDVGHHGVITLVGADGVARVRRTGDMDAMGEQVAGGKVYAAML Sbjct: 181 VVVVSMDPQYFTRLYHQIDVGHHGVITLVGADGVARVRRTGDMDAMGEQVAGGKVYAAML 240 Query: 241 AQGSGTIEAVSKIDGRERLYAFQKLRDYPLFAAVGIDLEERLAPYYRERERTLMLAALLS 300 AQGSGTIEAVSKIDGRERLYAFQKLRDYPLFAAVGIDL+ERLAPYYRERERTLMLAALLS Sbjct: 241 AQGSGTIEAVSKIDGRERLYAFQKLRDYPLFAAVGIDLKERLAPYYRERERTLMLAALLS 300 Query: 301 VAVLVFNAVLLWLAGSLVRSRREALQANQAKSRFLSNMSHEFRTPLNGVLGYSDALREEL 360 VAVLVFNAVLLWLAGSLVRSRREALQANQAKSRFLSNMSHEFRTPLNGVLGYSDALREEL Sbjct: 301 VAVLVFNAVLLWLAGSLVRSRREALQANQAKSRFLSNMSHEFRTPLNGVLGYSDALREEL 360 Query: 361 GESPLAQYAVAIHDSGNRLLDLVNAILEVTELEDRRVAVHLDPANIRELVGQAIARHYPQ 420 GESPLAQYAVAIHDSGNRLLDLVNAILEVTELEDRRVAVHLDPANIRELVGQAIARHYPQ Sbjct: 361 GESPLAQYAVAIHDSGNRLLDLVNAILEVTELEDRRVAVHLDPANIRELVGQAIARHYPQ 420 Query: 421 AQAKGLPLECRIGEDVPQILVCDERKIQRVLDNLISNAVRYTDHGRVMVEVERSGAQQLT 480 AQAKGLPLECRIGEDVPQILVCDERKIQRVLDNLISNAVRYTDHGRVMVEVERSG QQLT Sbjct: 421 AQAKGLPLECRIGEDVPQILVCDERKIQRVLDNLISNAVRYTDHGRVMVEVERSGPQQLT 480 Query: 481 MRIKDTGIGIAASQQNDIFEKFTQADDSPRRSQEGAGLGLTIAQRLVMLMGGQLVLQSEQ 540 MRIKDTGIGIAASQQNDIFEKFTQADDSPRRSQEGAGLGLTIAQRLVMLMGGQLVLQSEQ Sbjct: 481 MRIKDTGIGIAASQQNDIFEKFTQADDSPRRSQEGAGLGLTIAQRLVMLMGGQLVLQSEQ 540 Query: 541 HQGSTFSFTLPLQEPK 556 HQGSTFSFTLPLQEPK Sbjct: 541 HQGSTFSFTLPLQEPK 556 >gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729 [Herbaspirillum seropedicae SmR1] Length = 765 Score = 173 bits (439), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 147/539 (27%), Positives = 251/539 (46%), Gaps = 48/539 (8%) Query: 49 LDDARRDAQSLSRLFLEHAYRTIEAA-----------DQAALYLRYRYAERGNALNLATE 97 L+D R D +LS + E+ +T+ +A +QA + + ++ + Sbjct: 46 LNDWRNDLSTLSTVLAENTAQTMSSARLVIDSIQADIEQANPTDQQQLQRHVGSVEFSQI 105 Query: 98 IDNGLVARNVYNLFSIVDAQGDVVLSSK--PFTPVNLADREHVQVHMQGGQDQLFISKPV 155 + + + + +A+G ++ S+ P P++L DR++ H F+ + V Sbjct: 106 MREKIRGLPFISGIGVANAEGRILALSRVYPAPPIDLTDRDYYLHHRSSDSTADFVGETV 165 Query: 156 LGRVSKKWSLQITRRINRPDGGFGGVVVVSMDPQYFTRLYHQIDVGHHGVITLVGADGVA 215 L R + ++RR+N G ++V+ + +F + + I L DG Sbjct: 166 LSRAGGVPTFYVSRRVNDRQGRLLAIIVIGLPCAFFETFFDNVTRDKPFSIVLARKDG-- 223 Query: 216 RVRRTGDMDAMGEQVAGGKVYAAMLAQGSGTIEAVSKIDGRERLYAFQK--------LRD 267 + D + EQ G + LAQ ++ A +GR+ Q +R+ Sbjct: 224 --KTLTSTDLLPEQ--GQRRSITPLAQLHPSMPADD--EGRQSHVFMQSHWLGIHRPVRN 277 Query: 268 YPLFAAVGIDLEERLAPYYRERERTLMLAALLSVAVLVFNAVLLWL--------AGSLVR 319 +P++ +G+ E + E LML A +S+ L+ VL W A R Sbjct: 278 WPMYLEIGVTDEVYFGEWM-ESMYPLMLVAAISLVGLIGGFVLTWRQVRRREEDALLATR 336 Query: 320 SRREALQANQAKSRFLSNMSHEFRTPLNGVLGYSDALREELGESPLAQYAVAIHDSGNRL 379 + EA AN+AKS FL+ +SHE RTP+NG+LG S+ L E S YA +IH + L Sbjct: 337 LKEEADHANEAKSHFLAMVSHEIRTPMNGILGLSELLMESGLSSKQRDYADSIHGATGGL 396 Query: 380 LDLVNAILEVTELEDRRVAVHLDPANIRELVGQAIARHYPQAQAKGLPLECRIGEDVPQI 439 + ++N IL+ +++E ++ + + + LV + + P Q K + + + D P+ Sbjct: 397 VRIINDILDFSKIESGKLDLEMVAFHPVRLVQELSDLYRPSLQRKDVQFDLHL--DCPEN 454 Query: 440 L--VCDERKIQRVLDNLISNAVRYTDHGRVMVEV----ERSGAQ--QLTMRIKDTGIGIA 491 L V D ++++VL NL+SNA+++T G V + + + SG +L I DTGIGI+ Sbjct: 455 LSVVGDPARLKQVLGNLLSNAIKFTQAGHVQLSMSARQDESGPSRWRLFFAITDTGIGIS 514 Query: 492 ASQQNDIFEKFTQADDSPRRSQEGAGLGLTIAQRLVMLMGGQLVLQSEQHQGSTFSFTL 550 +F F QAD S R G GLGL I++ LV LMGG + QS + + FSF + Sbjct: 515 PEALQQLFRPFAQADSSISRRFGGTGLGLAISKNLVELMGGTIACQSIPGESTRFSFEI 573 >gi|300311948|ref|YP_003776040.1| sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1145 Score = 152 bits (384), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 88/239 (36%), Positives = 139/239 (58%), Gaps = 2/239 (0%) Query: 317 LVRSRREALQANQAKSRFLSNMSHEFRTPLNGVLGYSDALREELGESPLAQYAVA-IHDS 375 L R++ A AN AKSRF++ MSHE RTPLN +LGY+ L + P + A+ I S Sbjct: 674 LQRAKEAAESANLAKSRFVAGMSHELRTPLNSILGYAQILHVDATIPPARKDAIGVIRRS 733 Query: 376 GNRLLDLVNAILEVTELEDRRVAVHLDPANIRELVGQAIARHYPQAQAKGLPLECRIGED 435 G LL L++ +L++ ++E ++ + D + + Q + PQA+ KGL Sbjct: 734 GEHLLSLIDGLLDIAKIEAGKMRLASDELALEAFLEQIVGMFAPQAEQKGLHFRFDKSGR 793 Query: 436 VPQILVCDERKIQRVLDNLISNAVRYTDHGRVMVEVERSGAQQLTMRIKDTGIGIAASQQ 495 +PQI+ D+++++++L NL+ NAV++TDHG V + + R + + DTGIGIA + Sbjct: 794 LPQIVHADQKRLRQILINLLGNAVKFTDHGGVTLRL-RYAREIAYFDVIDTGIGIADDDR 852 Query: 496 NDIFEKFTQADDSPRRSQEGAGLGLTIAQRLVMLMGGQLVLQSEQHQGSTFSFTLPLQE 554 IF F ++ + R G GLGL I++ LV +MGG+L +QS+ QGS+F + L E Sbjct: 853 ERIFLPFERSSAANLREDIGTGLGLAISRMLVHIMGGELSVQSQVGQGSSFHLKIYLPE 911 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 145 bits (366), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 86/239 (35%), Positives = 136/239 (56%), Gaps = 5/239 (2%) Query: 317 LVRSRREALQANQAKSRFLSNMSHEFRTPLNGVLGYSDALREE----LGESPLAQYAVAI 372 LV E +ANQ KS FL+NMSHE RTPLN L + L + L + + ++A I Sbjct: 461 LVEKATELERANQYKSEFLANMSHELRTPLNSTLILAKLLADNKQGNLSDEQV-RFAQTI 519 Query: 373 HDSGNRLLDLVNAILEVTELEDRRVAVHLDPANIRELVGQAIARHYPQAQAKGLPLECRI 432 +GN LL L+N IL+++++E ++ + + + +L + A AQ KGL + + Sbjct: 520 SSAGNDLLALINDILDLSKMEAGKLDIVPESFPLTKLADELSASFAITAQQKGLGFKVEV 579 Query: 433 GEDVPQILVCDERKIQRVLDNLISNAVRYTDHGRVMVEVERSGAQQLTMRIKDTGIGIAA 492 VPQ + D +++ ++L NL+SNA+++T+ G V + + ++ ++DTGIGI Sbjct: 580 EPGVPQRMETDIQRLGQILKNLLSNAIKFTEKGEVSLRIYPDAGGRIAFAVRDTGIGIGE 639 Query: 493 SQQNDIFEKFTQADDSPRRSQEGAGLGLTIAQRLVMLMGGQLVLQSEQHQGSTFSFTLP 551 Q IFE F QAD S R G GLGL+I++ L L+GG + + S +GS F+ +LP Sbjct: 640 DQHQFIFEAFRQADGSTHRKYGGTGLGLSISRDLAHLLGGDIRVTSRLGEGSIFTLSLP 698 >gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 723 Score = 131 bits (329), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 91/268 (33%), Positives = 147/268 (54%), Gaps = 13/268 (4%) Query: 293 LMLAALLSVAVLVFNAVL---LWLAGSLVRSRREALQANQAKSRFLSNMSHEFRTPLNGV 349 L+LAA+ + L +L A LV+++ A A + FLS MSHE RTP+N V Sbjct: 46 LVLAAIWGIHALTLLRLLRENARTAEKLVQAKEAADHAMLTRGAFLSTMSHEIRTPMNAV 105 Query: 350 LGYSDALREELGESPLAQYAVAIHDSGNRLLDLVNAILEVTELEDRRV---AVHLDPANI 406 LG + LR ++ A++A AI DS + LL +VN +L+ ++++ ++ V D A + Sbjct: 106 LGMAGLLRSTRLDTQQAEFAKAIEDSAHALLGIVNDVLDFSKIDVGKMDIAVVEFDLAKV 165 Query: 407 RELVGQAIARHYPQAQAKGLPLECRIGEDVPQILVCDERKIQRVLDNLISNAVRYTDHGR 466 E +A++ +A+ K + L + +P LV D +I+++L NL+ NA+++T G Sbjct: 166 VEASAEALSF---KAREKRVRLLAHLDSRLPPRLVGDPGRIRQILLNLLGNALKFTHAGE 222 Query: 467 VMVEVE----RSGAQQLTMRIKDTGIGIAASQQNDIFEKFTQADDSPRRSQEGAGLGLTI 522 V++ V+ S ++ ++D+GIG+ +F F QAD S R G GLGL+I Sbjct: 223 VLLRVQALQVTSDHCRVRFEVRDSGIGMDEDTLRRLFSPFAQADSSVTRQYGGTGLGLSI 282 Query: 523 AQRLVMLMGGQLVLQSEQHQGSTFSFTL 550 +RLV LMGG + ++S GS F F L Sbjct: 283 CKRLVELMGGSIGVESALGTGSCFWFEL 310 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,610,325 Number of extensions: 68516 Number of successful extensions: 356 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 310 Number of HSP's successfully gapped: 45 Length of query: 556 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 466 Effective length of database: 1,193,720 Effective search space: 556273520 Effective search space used: 556273520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)