BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0072.p (765 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729... 1551 0.0 gi|300313029|ref|YP_003777121.1| two component hybrid sensor his... 244 2e-65 gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kin... 214 1e-56 gi|300311948|ref|YP_003776040.1| sensor histidine kinase/respons... 182 1e-46 gi|300312677|ref|YP_003776769.1| two component hybrid sensor his... 176 5e-45 >gi|300313023|ref|YP_003777115.1| hypothetical protein Hsero_3729 [Herbaspirillum seropedicae SmR1] Length = 765 Score = 1551 bits (4016), Expect = 0.0, Method: Compositional matrix adjust. Identities = 758/765 (99%), Positives = 761/765 (99%) Query: 1 MNEPLPTDQRKTPRMRISALTVGALLLILLTVTVIVSGTLLYRASLNDWRNDLSTLSTVL 60 MNEPLP DQRKTPRMRISALTVGALLLILLTVTVIVSGTLLYRASLNDWRNDLSTLSTVL Sbjct: 1 MNEPLPNDQRKTPRMRISALTVGALLLILLTVTVIVSGTLLYRASLNDWRNDLSTLSTVL 60 Query: 61 AENTAQTMSSARLVIDSIQADIEQANPADQQQLQRHVGSVEFSQIMREKIRGLPFISGIG 120 AENTAQTMSSARLVIDSIQADIEQANP DQQQLQRHVGSVEFSQIMREKIRGLPFISGIG Sbjct: 61 AENTAQTMSSARLVIDSIQADIEQANPTDQQQLQRHVGSVEFSQIMREKIRGLPFISGIG 120 Query: 121 VANAEGRILALSRVYPAPPIDLTDRDYYLYHRNSDSTADFVGETVLSRAGGVPTFYVSRR 180 VANAEGRILALSRVYPAPPIDLTDRDYYL+HR+SDSTADFVGETVLSRAGGVPTFYVSRR Sbjct: 121 VANAEGRILALSRVYPAPPIDLTDRDYYLHHRSSDSTADFVGETVLSRAGGVPTFYVSRR 180 Query: 181 VNDRQGRLLAIIVIGLPCAFFETFFDNVTRDKPFSIVLARKDGKTLTSTDLLPEQGQRRS 240 VNDRQGRLLAIIVIGLPCAFFETFFDNVTRDKPFSIVLARKDGKTLTSTDLLPEQGQRRS Sbjct: 181 VNDRQGRLLAIIVIGLPCAFFETFFDNVTRDKPFSIVLARKDGKTLTSTDLLPEQGQRRS 240 Query: 241 ITPLAQLHPSMPADDEGRQSHVFMQSHWLGIHRPVRNWPMYLEIGVTDEVYFGEWMESMY 300 ITPLAQLHPSMPADDEGRQSHVFMQSHWLGIHRPVRNWPMYLEIGVTDEVYFGEWMESMY Sbjct: 241 ITPLAQLHPSMPADDEGRQSHVFMQSHWLGIHRPVRNWPMYLEIGVTDEVYFGEWMESMY 300 Query: 301 PLMLVAAISLVGLIGGFVLTWRQVRRREEDALLATRLKEEADHANEAKSHFLAMVSHEIR 360 PLMLVAAISLVGLIGGFVLTWRQVRRREEDALLATRLKEEADHANEAKSHFLAMVSHEIR Sbjct: 301 PLMLVAAISLVGLIGGFVLTWRQVRRREEDALLATRLKEEADHANEAKSHFLAMVSHEIR 360 Query: 361 TPMNGILGLSELLMESGLSSKQRDYADSIHGATGGLVRIINDILDFSKIESGKLDLEMVA 420 TPMNGILGLSELLMESGLSSKQRDYADSIHGATGGLVRIINDILDFSKIESGKLDLEMVA Sbjct: 361 TPMNGILGLSELLMESGLSSKQRDYADSIHGATGGLVRIINDILDFSKIESGKLDLEMVA 420 Query: 421 FHPVRLVQELSDLYRPSLQRKDVQFDLHLDCPENLSVVGDPARLKQVLGNLLSNAIKFTQ 480 FHPVRLVQELSDLYRPSLQRKDVQFDLHLDCPENLSVVGDPARLKQVLGNLLSNAIKFTQ Sbjct: 421 FHPVRLVQELSDLYRPSLQRKDVQFDLHLDCPENLSVVGDPARLKQVLGNLLSNAIKFTQ 480 Query: 481 AGHVQLSMSARQDESGPSRWRLFFAITDTGIGISPEALQQLFRPFAQADSSISRRFGGTG 540 AGHVQLSMSARQDESGPSRWRLFFAITDTGIGISPEALQQLFRPFAQADSSISRRFGGTG Sbjct: 481 AGHVQLSMSARQDESGPSRWRLFFAITDTGIGISPEALQQLFRPFAQADSSISRRFGGTG 540 Query: 541 LGLAISKNLVELMGGTIACQSIPGESTRFSFELGCAEASTPAPEPVSEAAAPAPATALAT 600 LGLAISKNLVELMGGTIACQSIPGESTRFSFE+ C EASTPAPEPVSEAAAPAPATALAT Sbjct: 541 LGLAISKNLVELMGGTIACQSIPGESTRFSFEILCPEASTPAPEPVSEAAAPAPATALAT 600 Query: 601 TAPALPPAAEAPGTLMPPDNAPQPPSNVDASATVLPSLLAGARILMAEDTEINRQLLRIL 660 TAPALPPAAEAPGTLMPPDNAPQPPSNVDASATVLPSLLAGARILMAEDTEINRQLLRIL Sbjct: 601 TAPALPPAAEAPGTLMPPDNAPQPPSNVDASATVLPSLLAGARILMAEDTEINRQLLRIL 660 Query: 661 LARKGCILQEVENGLQAVEAIRTGHYDLVLMDCMMPIMDGYQATAEIRAHEAARGLPRTP 720 LARKGCILQEVENGLQAVEAIRTGHYDLVLMDCMMPIMDGYQATAEIRAHEAARGLPRTP Sbjct: 661 LARKGCILQEVENGLQAVEAIRTGHYDLVLMDCMMPIMDGYQATAEIRAHEAARGLPRTP 720 Query: 721 IIALTASAIEGDRQRCLDAGMDDYLSKPFTQVGLMQTVEKWLQKS 765 IIALTASAIEGDRQRCLDAGMDDYLSKPFTQVGLMQTVEKWLQKS Sbjct: 721 IIALTASAIEGDRQRCLDAGMDDYLSKPFTQVGLMQTVEKWLQKS 765 >gi|300313029|ref|YP_003777121.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1524 Score = 244 bits (622), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 172/425 (40%), Positives = 238/425 (56%), Gaps = 30/425 (7%) Query: 329 EDALLATRLKEEADHANEAKSHFLAMVSHEIRTPMNGILGLSELLMESGLSSKQRDYADS 388 E+ L+ RLK A+ AN AKS FLA VSHEIRTPMNG+LG+ +LL + Q+DY D Sbjct: 880 ENNLVDARLK--AEQANSAKSLFLASVSHEIRTPMNGVLGMIQLLQRTQPLPAQQDYLDK 937 Query: 389 IHGATGGLVRIINDILDFSKIESGKLDLEMVAFHPVRLVQELSDLYRPSLQRKDVQFDLH 448 + L+ ++ND+LDFSK+++ +L L++ AF R L+ + S + V + Sbjct: 938 AEISAKSLLNLLNDLLDFSKMDAKQLQLDLQAFELERFGHNLAAVLAGSPPQAGVSLHIA 997 Query: 449 LDCPENLSVVGDPARLKQVLGNLLSNAIKFTQAGHVQLSMSARQDESGPSRWRLFFAITD 508 LD +VGD RL+QVL NL NA+KFT GH+ + + R D L ++D Sbjct: 998 LDPQLPRRLVGDAHRLQQVLVNLTGNALKFTHQGHIDVHFT-RLDPLPGRTVALRVEVSD 1056 Query: 509 TGIGISPEALQQLFRPFAQADSSISRRFGGTGLGLAISKNLVELMGGTIACQSIPGESTR 568 TGIGI+ + L +F F QA++S +RR+GGTGLGLAISK+LV LMGG + S PG +R Sbjct: 1057 TGIGIAADRLDDIFDAFTQAEASTTRRYGGTGLGLAISKHLVGLMGGQLQVSSEPGRGSR 1116 Query: 569 FSFELGCA--EASTPAPEPVSEAAAPAPATALATTAPALPPAAEAPGTLMPPDNAPQPPS 626 F F+L A + S P +AA P P A + TA + AP T +A Sbjct: 1117 FWFDLQLAVDDDSVLGPAAADDAARPQPLQAASHTA-----GSRAPETARSAPDA----- 1166 Query: 627 NVDASATVLPSLLAGARILMAEDTEINRQLLRILLARKGCILQEVENGLQ--AVEAIRTG 684 L G +IL+ ED +NRQ+ LLA G + ++G A+ Sbjct: 1167 ----------QCLQGVQILLVEDNPVNRQVASELLALSGAQVTCADSGSMGVAMACDPAR 1216 Query: 685 HYDLVLMDCMMPIMDGYQATAEIRAHEAARGLPRTPIIALTASAIEGDRQRCLDAGMDDY 744 H+DLVLMD MP MDG +AT IR RGL PI+A+TA+A D+QRC++AGM + Sbjct: 1217 HFDLVLMDLQMPGMDGIEATRRIRGQYELRGL---PILAMTANASPEDKQRCIEAGMAGH 1273 Query: 745 LSKPF 749 ++KP Sbjct: 1274 IAKPI 1278 >gi|300310566|ref|YP_003774658.1| Hpt sensor hybrid histidine kinase [Herbaspirillum seropedicae SmR1] Length = 723 Score = 214 bits (546), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 121/277 (43%), Positives = 175/277 (63%), Gaps = 7/277 (2%) Query: 308 ISLVGLIGGFVLTWRQVRRREEDALLATRL---KEEADHANEAKSHFLAMVSHEIRTPMN 364 + L + G LT ++ R E+A A +L KE ADHA + FL+ +SHEIRTPMN Sbjct: 46 LVLAAIWGIHALTLLRLLR--ENARTAEKLVQAKEAADHAMLTRGAFLSTMSHEIRTPMN 103 Query: 365 GILGLSELLMESGLSSKQRDYADSIHGATGGLVRIINDILDFSKIESGKLDLEMVAFHPV 424 +LG++ LL + L ++Q ++A +I + L+ I+ND+LDFSKI+ GK+D+ +V F Sbjct: 104 AVLGMAGLLRSTRLDTQQAEFAKAIEDSAHALLGIVNDVLDFSKIDVGKMDIAVVEFDLA 163 Query: 425 RLVQELSDLYRPSLQRKDVQFDLHLDCPENLSVVGDPARLKQVLGNLLSNAIKFTQAGHV 484 ++V+ ++ + K V+ HLD +VGDP R++Q+L NLL NA+KFT AG V Sbjct: 164 KVVEASAEALSFKAREKRVRLLAHLDSRLPPRLVGDPGRIRQILLNLLGNALKFTHAGEV 223 Query: 485 QLSMSARQDESGPSRWRLFFAITDTGIGISPEALQQLFRPFAQADSSISRRFGGTGLGLA 544 L + A Q S R R F + D+GIG+ + L++LF PFAQADSS++R++GGTGLGL+ Sbjct: 224 LLRVQALQVTSDHCRVR--FEVRDSGIGMDEDTLRRLFSPFAQADSSVTRQYGGTGLGLS 281 Query: 545 ISKNLVELMGGTIACQSIPGESTRFSFELGCAEASTP 581 I K LVELMGG+I +S G + F FELG + P Sbjct: 282 ICKRLVELMGGSIGVESALGTGSCFWFELGIPVTTGP 318 Score = 98.6 bits (244), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 51/119 (42%), Positives = 73/119 (61%), Gaps = 2/119 (1%) Query: 644 ILMAEDTEINRQLLRILLARKGCILQEVENGLQAVEAIRTGHYDLVLMDCMMPIMDGYQA 703 IL+ ED +N+++ L G NGL+A+E + Y LVLMDC MP++DGY+ Sbjct: 478 ILLVEDNVMNQKVAVYQLNAMGYAADIAANGLEALERLARHRYALVLMDCQMPLLDGYET 537 Query: 704 TAEIRAHEAARGLPRTPIIALTASAIEGDRQRCLDAGMDDYLSKPFTQVGLMQTVEKWL 762 T IR + L T I+A+TA+A+ GDR+RCL+AGMDDYL+KP + L + +L Sbjct: 538 TRRIRRMQGE--LAHTTIVAMTANAMAGDRERCLEAGMDDYLTKPLLRSQLSAMLAHYL 594 >gi|300311948|ref|YP_003776040.1| sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1145 Score = 182 bits (461), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 133/439 (30%), Positives = 218/439 (49%), Gaps = 56/439 (12%) Query: 326 RREEDALLATRLKEEADHANEAKSHFLAMVSHEIRTPMNGILGLSELL-MESGLSSKQRD 384 ++ DA L R KE A+ AN AKS F+A +SHE+RTP+N ILG +++L +++ + ++D Sbjct: 667 HKQTDAEL-QRAKEAAESANLAKSRFVAGMSHELRTPLNSILGYAQILHVDATIPPARKD 725 Query: 385 YADSIHGATGGLVRIINDILDFSKIESGKLDLEMVAFHPVRLVQELSDLYRPSLQRKDVQ 444 I + L+ +I+ +LD +KIE+GK+ L ++++ ++ P ++K + Sbjct: 726 AIGVIRRSGEHLLSLIDGLLDIAKIEAGKMRLASDELALEAFLEQIVGMFAPQAEQKGLH 785 Query: 445 FDLHLDCPENLSVVGDPARLKQVLGNLLSNAIKFTQAGHVQLSMSARQDESGPSRWRLFF 504 F V D RL+Q+L NLL NA+KFT G V L + ++ + +F Sbjct: 786 FRFDKSGRLPQIVHADQKRLRQILINLLGNAVKFTDHGGVTLRLRYAREIA-------YF 838 Query: 505 AITDTGIGISPEALQQLFRPFAQADSSISRRFGGTGLGLAISKNLVELMGGTIACQSIPG 564 + DTGIGI+ + +++F PF ++ ++ R GTGLGLAIS+ LV +MGG ++ QS G Sbjct: 839 DVIDTGIGIADDDRERIFLPFERSSAANLREDIGTGLGLAISRMLVHIMGGELSVQSQVG 898 Query: 565 ESTRFSFELGCAEASTPAPEPVSEAAAPAPATALATTAPALPPAAEAPGTLMPPDNAPQP 624 + + F ++ E P P E A Sbjct: 899 QGSSFHLKIYLPEVHVPRPRIKLEDQMSGYAG---------------------------- 930 Query: 625 PSNVDASATVLPSLLAGARILMAEDTEINRQLLRILLARKGCILQEVENGLQAVEAIRTG 684 PS IL+ +D R+LL+ +L G + E ++GL +E + Sbjct: 931 PSR---------------HILVVDDQASQRRLLKDMLMPLGFTVSEADSGLACMETLGHA 975 Query: 685 HYDLVLMDCMMPIMDGYQATAEIRAHEAARGLPRTPIIALTASAIEGDRQRCLDAGMDDY 744 DLVL+D MP MDG+ IR ARGL + PI+ ++A+A E +R ++D+ Sbjct: 976 LPDLVLLDIAMPQMDGWSVARAIR----ARGLAQLPILLVSANAFENLHERNEPPLVNDF 1031 Query: 745 LSKPFTQVGLMQTVEKWLQ 763 + KP + L+ + + LQ Sbjct: 1032 VVKPVSYHELLGKIRQHLQ 1050 >gi|300312677|ref|YP_003776769.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1160 Score = 176 bits (447), Expect = 5e-45, Method: Compositional matrix adjust. Identities = 152/447 (34%), Positives = 225/447 (50%), Gaps = 50/447 (11%) Query: 340 EADHANEAKSHFLAMVSHEIRTPMNGILGLSELLMES---GLSSKQRDYADSIHGATGGL 396 E + AN+ KS FLA +SHE+RTP+N L L++LL ++ LS +Q +A +I A L Sbjct: 467 ELERANQYKSEFLANMSHELRTPLNSTLILAKLLADNKQGNLSDEQVRFAQTISSAGNDL 526 Query: 397 VRIINDILDFSKIESGKLDLEMVAFHPVRLVQELSDLYRPSLQRKDVQFDLHLD--CPEN 454 + +INDILD SK+E+GKLD+ +F +L ELS + + Q+K + F + ++ P+ Sbjct: 527 LALINDILDLSKMEAGKLDIVPESFPLTKLADELSASFAITAQQKGLGFKVEVEPGVPQR 586 Query: 455 LSVVGDPARLKQVLGNLLSNAIKFTQAGHVQLSMSARQDESGPSRWRLFFAITDTGIGIS 514 + D RL Q+L NLLSNAIKFT+ G V L + D G R+ FA+ DTGIGI Sbjct: 587 MET--DIQRLGQILKNLLSNAIKFTEKGEVSLRIYP--DAGG----RIAFAVRDTGIGIG 638 Query: 515 PEALQQLFRPFAQADSSISRRFGGTGLGLAISKNLVELMGGTIACQSIPGESTRFSFELG 574 + Q +F F QAD S R++GGTGLGL+IS++L L+GG I S GE + F+ L Sbjct: 639 EDQHQFIFEAFRQADGSTHRKYGGTGLGLSISRDLAHLLGGDIRVTSRLGEGSIFTLSLP 698 Query: 575 CAEASTPAPEPVSEAAAPAPATAL-------ATTAPAL----PPAAEAPGTLMPPDNAPQ 623 +S A + PA A A TAP + PAA AP L + Sbjct: 699 PLYSSEQAVSNTAPEPTPAAAAAASTLQAAHVATAPDVRINRTPAAAAPQRLQEI-SIDD 757 Query: 624 PPSNVDASATVLPSLLAGARILMAEDTEINRQLLRILLARKG--CILQEVEN-GLQAVEA 680 ++ S+ + IL+ ED +LR L G C++ N G+ A E Sbjct: 758 DRDRIETSSRL---------ILVIEDDAAFAMILRDLAREMGFQCVVTHSANEGVAAAEM 808 Query: 681 IRTGHYDLVLMDCMMPIMDGYQATAEIRAHEAARGLPRTPIIALTASAIEGDRQRCLDAG 740 R +LMD +P G +I+ + P+T I + ++ ++ G Sbjct: 809 YRPSA---ILMDMNLPDFSGLGVLDQIKRN------PKTRHIPVHVVSVADYSHEAMERG 859 Query: 741 MDDYLSKPFTQ---VGLMQTVE-KWLQ 763 Y KP + +G ++ +E K+LQ Sbjct: 860 AIGYALKPVQREELIGAIRKLEAKFLQ 886 Score = 87.8 bits (216), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 53/134 (39%), Positives = 75/134 (55%), Gaps = 9/134 (6%) Query: 637 SLLAGARILMAEDTEINRQLLRILLARKGCILQEVENGLQAVEAIRTGH------YDLVL 690 ++ G RIL+ ED N L +L KG ++ NG +A++A++ DLVL Sbjct: 1030 TVFEGRRILVVEDDVRNIFALSSVLEPKGMKVEIARNGREALQALQRSQDKPDAPIDLVL 1089 Query: 691 MDCMMPIMDGYQATAEIRAHEAARGLPRTPIIALTASAIEGDRQRCLDAGMDDYLSKPFT 750 MD MMP MDG A EIR + R PIIALTA A++ D+++CL AG +DY++KP Sbjct: 1090 MDIMMPEMDGLTAMREIRKQAVWK---RLPIIALTAKAMKDDQEKCLAAGANDYIAKPLD 1146 Query: 751 QVGLMQTVEKWLQK 764 L+ V W+ K Sbjct: 1147 VEKLLSLVRVWMPK 1160 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,146,939 Number of extensions: 85733 Number of successful extensions: 543 Number of sequences better than 1.0e-08: 64 Number of HSP's gapped: 482 Number of HSP's successfully gapped: 67 Length of query: 765 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 672 Effective length of database: 1,179,515 Effective search space: 792634080 Effective search space used: 792634080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)