BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0036.0069.p (308 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313026|ref|YP_003777118.1| signal transduction protein con... 624 e-180 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 183 1e-47 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 176 3e-45 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 174 9e-45 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 171 9e-44 >gi|300313026|ref|YP_003777118.1| signal transduction protein containing sensor and EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 544 Score = 624 bits (1608), Expect = e-180, Method: Compositional matrix adjust. Identities = 307/308 (99%), Positives = 307/308 (99%) Query: 1 MTGQQRWFLPVGILLGIGLVMLVLRVSQARLSPLADLQLAVKKKEFIVHYQPIIEMASGR 60 MTGQQRWFLPVGILLGIGLVMLVLRVSQARLSPLADLQLAVKKKEFIVHYQPIIEMASGR Sbjct: 237 MTGQQRWFLPVGILLGIGLVMLVLRVSQARLSPLADLQLAVKKKEFIVHYQPIIEMASGR 296 Query: 61 CIGAEALLRWRRPDGSIVRPDFFIALAEESGLILPLTDLVVDTVVREMRDTLRQDRALHI 120 CIGAEALLRWRRPDGSIVRPDFFIALAEESGLILPLTDLVVDTVVREMRDTLRQDRALHI Sbjct: 297 CIGAEALLRWRRPDGSIVRPDFFIALAEESGLILPLTDLVVDTVVREMRDTLRQDRALHI 356 Query: 121 AINVAAQDMKTGRILEVVSQALAGSDIRPEQLWMEATERGCMDIASASLTLARAREMGHS 180 AINVAAQDMKTGRILEVVSQALAGSDIRPEQLWMEATERGCMDIASASLTLARAREMGHS Sbjct: 357 AINVAAQDMKTGRILEVVSQALAGSDIRPEQLWMEATERGCMDIASASLTLARAREMGHS 416 Query: 181 MALDDFGTGYSNLQYLQGLPMDALKIDKSFVSTIGTGAATSAVIDHIIAMAKSLKLYIVA 240 MALDDFGTGYSNLQYLQGLPMDALKIDKSFVSTIGTGAATSAVIDHIIAMAKSLKLYIVA Sbjct: 417 MALDDFGTGYSNLQYLQGLPMDALKIDKSFVSTIGTGAATSAVIDHIIAMAKSLKLYIVA 476 Query: 241 EGVETLEQAAYLAERGVDFAQGWLFSRPLPVEEFVAFYCATLADYGSGPEIIRGPQPAST 300 EGVETLEQAAYLAERGVDFAQGWLFSRPLPVEEFVAFYCATLADYGSGPEIIRGPQPAST Sbjct: 477 EGVETLEQAAYLAERGVDFAQGWLFSRPLPVEEFVAFYCATLADYGSGPEIIRGPQPAST 536 Query: 301 DCPAALDA 308 D PAALDA Sbjct: 537 DSPAALDA 544 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 183 bits (465), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 102/247 (41%), Positives = 152/247 (61%), Gaps = 2/247 (0%) Query: 30 RLSPLADLQLAVKKKEFIVHYQPIIEMASGRCIGAEALLRWRRPDGSIVRPDFFIALAEE 89 RL DL+ A+ EF++HYQP +++ASGR IG+EAL+RW+ P +V P FI LAEE Sbjct: 691 RLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLAEE 750 Query: 90 SGLILPLTDLVVDTVVREMRD-TLRQDRALHIAINVAAQDMKTGRILEVVSQALAGSDIR 148 +GLILP+ V+ + R L + +++N++ ++ ++ L + + Sbjct: 751 TGLILPIGAWVLRAACTQCRQWQLGGYADISVSVNLSVGQFLQKDLVSSIADVLEDTGLP 810 Query: 149 PEQLWMEATERGCM-DIASASLTLARAREMGHSMALDDFGTGYSNLQYLQGLPMDALKID 207 PE L +E TE M D+ A LA +++G +++DDFGTGYS+L YL+ P+D LKID Sbjct: 811 PESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPIDVLKID 870 Query: 208 KSFVSTIGTGAATSAVIDHIIAMAKSLKLYIVAEGVETLEQAAYLAERGVDFAQGWLFSR 267 +SFV I A +A+ II++A+ LKL ++AEGVET EQ YL G D QG+LFS Sbjct: 871 RSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYLFSP 930 Query: 268 PLPVEEF 274 P+P ++F Sbjct: 931 PVPADKF 937 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 176 bits (445), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 99/249 (39%), Positives = 151/249 (60%), Gaps = 4/249 (1%) Query: 30 RLSPLADLQLAVKKKEFIVHYQPIIEMASGRCIGAEALLRWRRPDGSIVRPDFFIALAEE 89 R++ A+L+ A+++ EF+VHYQP IE+A GR +GAEAL+RW+ P ++ P FI LAEE Sbjct: 842 RIALEAELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGEFITLAEE 901 Query: 90 SGLILPLTDLVVDTVVREMRDTLRQDRAL--HIAINVAAQDMKTGRILEVVSQALAGSDI 147 + LI + LV+D R++ +RQ + I+IN+ R+L+ + + + Sbjct: 902 TRLIDQIGLLVLDVACRDI-GLMRQQQMTCGRISINLTGSQFGDERLLDDIKEVVDRHGT 960 Query: 148 RPEQLWMEATERGCMDIASASLTLARA-REMGHSMALDDFGTGYSNLQYLQGLPMDALKI 206 P L E TE M ++ + R G ++++DDFGTGYS+L YL+ P++ LKI Sbjct: 961 PPSCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRFPVNTLKI 1020 Query: 207 DKSFVSTIGTGAATSAVIDHIIAMAKSLKLYIVAEGVETLEQAAYLAERGVDFAQGWLFS 266 DKSF++ I +SA++ IIAM+ +L L +V EGVET +Q L E G D QG+ FS Sbjct: 1021 DKSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDEYQGYYFS 1080 Query: 267 RPLPVEEFV 275 +P+P +FV Sbjct: 1081 KPVPYAQFV 1089 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 174 bits (441), Expect = 9e-45, Method: Compositional matrix adjust. Identities = 102/257 (39%), Positives = 152/257 (59%), Gaps = 17/257 (6%) Query: 30 RLSPLADLQLAVKKKEFIVHYQPIIEMASGRCIGAEALLRWRRPDGSIVRPDFFIALAEE 89 RL L +L+ A+ + EF ++YQP + + GR IGAEAL+RW P +V PD FI AEE Sbjct: 439 RLEMLINLRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFIPFAEE 498 Query: 90 SGLILPLTDLVVDTVVREMRDTLRQDRALH--------IAINVAAQDMKTGRILEVVSQA 141 +GLI+P+ + V +R RQ++A +A+N++ + + + + V+ Sbjct: 499 AGLIVPIGEWV-------LRTACRQNQAWQQAGLPPIPVAVNLSPRQLNQS-LPDFVAGV 550 Query: 142 LAGSDIRPEQLWMEATERGCMDIASASL-TLARAREMGHSMALDDFGTGYSNLQYLQGLP 200 LA S + L +E TE M A S+ TL + +G +++DDFGTGYS+L YL+ P Sbjct: 551 LAQSGLAASWLELEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFP 610 Query: 201 MDALKIDKSFVSTIGTGAATSAVIDHIIAMAKSLKLYIVAEGVETLEQAAYLAERGVDFA 260 +DALKIDKSFV I A ++A++ II++A L L ++AEGVE EQ A+L E D Sbjct: 611 VDALKIDKSFVRDIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACDEV 670 Query: 261 QGWLFSRPLPVEEFVAF 277 QG+ F +P+ V F A+ Sbjct: 671 QGYYFGKPMEVAVFTAW 687 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 171 bits (432), Expect = 9e-44, Method: Compositional matrix adjust. Identities = 97/240 (40%), Positives = 143/240 (59%), Gaps = 2/240 (0%) Query: 38 QLAVKKKEFIVHYQPIIEMASGRCIGAEALLRWRRPDGSIVRPDFFIALAEESGLILPLT 97 ++A+++ +F + YQP + +G +GAEALLRW P +V PD FI LAE SGLILP+ Sbjct: 445 RVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQFIGLAERSGLILPIG 504 Query: 98 DLVVDTVVREMRDTLRQDRA-LHIAINVAAQDMKTGRILEVVSQALAGSDIRPEQLWMEA 156 V+D R+MR+ +++N++A +LEVV L + P L +E Sbjct: 505 AWVLDEACRQMREWFALGYVDWTMSVNLSALQFADPHLLEVVRTTLERHQLPPPCLTLEV 564 Query: 157 TERGCMDIASASL-TLARAREMGHSMALDDFGTGYSNLQYLQGLPMDALKIDKSFVSTIG 215 TE M ASL TL + ++G +++DDFGTGYS+L YL+ LP + LKID+ FV + Sbjct: 565 TESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLPANELKIDRGFVRELR 624 Query: 216 TGAATSAVIDHIIAMAKSLKLYIVAEGVETLEQAAYLAERGVDFAQGWLFSRPLPVEEFV 275 +A++ I+A+ ++L L IVAEGVET+EQ +L G D QG+L RP+ E F+ Sbjct: 625 QDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDALQGYLMGRPMTPELFL 684 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 862,849 Number of extensions: 33254 Number of successful extensions: 157 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 135 Number of HSP's successfully gapped: 18 Length of query: 308 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 223 Effective length of database: 1,217,395 Effective search space: 271479085 Effective search space used: 271479085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)